Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 491,302 G→C 6.4% intergenic (+60/‑272) metQ_1 → / → sle1_1 putative D‑methionine‑binding lipoprotein MetQ/N‑acetylmuramoyl‑L‑alanine amidase sle1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE491,3020GC 6.4% 455.6 / 15.5 139intergenic (+60/‑272)metQ_1/sle1_1putative D‑methionine‑binding lipoprotein MetQ/N‑acetylmuramoyl‑L‑alanine amidase sle1
Reads supporting (aligned to +/- strand):  ref base G (67/61);  new base C (7/2);  total (74/65)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATAC  >  USA300TCH1516_ALE/491169‑491438
                                                                                                                                     |                                                                                                                                        
tCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATaaaa                                                                                                                                   <  1:286100/141‑1 (MQ=255)
 cAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAat                                                                                                                                  <  1:339586/141‑1 (MQ=255)
  aaaGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAatt                                                                                                                                 >  1:226538/1‑141 (MQ=255)
     gATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAattatt                                                                                                                              >  1:496483/1‑141 (MQ=255)
      aTGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTAttt                                                                                                                             <  1:939655/141‑1 (MQ=255)
           gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                        >  1:460150/1‑141 (MQ=255)
           gagaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAAt                                                                                                                        >  1:678601/1‑141 (MQ=255)
             gaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATag                                                                                                                      <  2:1052677/141‑1 (MQ=255)
             gaAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATag                                                                                                                      <  2:404141/141‑1 (MQ=255)
               aaCCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATagag                                                                                                                    <  3:336429/141‑1 (MQ=255)
                 ccTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGaa                                                                                                                  >  2:1042017/1‑141 (MQ=255)
                  cTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGaaa                                                                                                                 >  4:221314/1‑141 (MQ=255)
                   tGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGTCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAg                                                                                                                >  1:102537/1‑141 (MQ=255)
                    gTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAgg                                                                                                               >  1:549361/1‑141 (MQ=255)
                    gTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAgg                                                                                                               >  2:358131/1‑141 (MQ=255)
                    gTTAATTTATCAAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAgg                                                                                                               >  4:131689/1‑141 (MQ=255)
                       aaTTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGAt                                                                                                            <  4:123906/141‑1 (MQ=255)
                             tCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAAt                                                                                                      <  2:940976/141‑1 (MQ=255)
                              cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATc                                                                                                     >  2:342731/1‑141 (MQ=255)
                              cTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATc                                                                                                     <  1:61154/141‑1 (MQ=255)
                                     tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                              <  2:905027/141‑1 (MQ=255)
                                     tGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTtat                                                                                              <  2:905119/141‑1 (MQ=255)
                                        gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                           >  2:280327/1‑141 (MQ=255)
                                        gaTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGt                                                                                           >  2:763428/1‑141 (MQ=255)
                                         aTTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTg                                                                                          >  3:216200/1‑141 (MQ=255)
                                          ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTCGGGCGTTATTGTTGGAGACTAAAATTCTTTCCAATAGAGAAGGGGATTGTAATCATTTTATAGTGa                                                                                         <  1:973687/141‑1 (MQ=255)
                                          ttAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGa                                                                                         >  4:166125/1‑141 (MQ=255)
                                          ttAAAGAAATAGAAACGTCATTAGCAAAATAAATAATATTGCATACAACAAGCAAAGTTCATGCTTATGTTAGTCGGAAGATAATGTTGGAGCCTACAATTATTATCAATCCAGAAAGGGACTTAAATCATTTTATAGTGa                                                                                         <  1:973900/141‑1 (MQ=255)
                                              ggCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTACATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTGTTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAata                                                                                     >  3:16494/1‑141 (MQ=255)
                                               gCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAatat                                                                                    >  2:891724/1‑141 (MQ=255)
                                                cAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAAtatt                                                                                   >  4:108430/1‑141 (MQ=255)
                                                   tAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATg                                                                                >  1:111236/1‑141 (MQ=255)
                                                     gAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGaa                                                                              >  1:1020180/1‑141 (MQ=255)
                                                         cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                          <  1:864893/141‑1 (MQ=255)
                                                         cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                          <  1:325299/141‑1 (MQ=255)
                                                         cGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTg                                                                          <  4:7789/141‑1 (MQ=255)
                                                          gTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGt                                                                         <  3:224920/141‑1 (MQ=255)
                                                           tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                        <  1:2711/141‑1 (MQ=255)
                                                           tCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGta                                                                        <  1:2717/141‑1 (MQ=255)
                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  1:124287/1‑141 (MQ=255)
                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  4:18305/1‑141 (MQ=255)
                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  2:993274/1‑141 (MQ=255)
                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       <  3:32101/141‑1 (MQ=255)
                                                            cATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTACGTTTGTAGGGCGTTATTGTTTGACAATAAAATTATTTCCAATAGAGAAAGGGAATGTAATCATTTTATAGTGAAATATTATGAAATTGtaa                                                                       >  2:84968/1‑141 (MQ=255)
                                                             aTTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGtaat                                                                      >  1:532624/1‑141 (MQ=255)
                                                                aGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTaataat                                                                   <  2:169965/141‑1 (MQ=255)
                                                                   aaaaTAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTa                                                                <  1:423623/141‑1 (MQ=255)
                                                                     aataaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGa                                                              >  2:409367/1‑141 (MQ=255)
                                                                       taaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGata                                                            >  2:1041924/1‑141 (MQ=255)
                                                                        aaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGatat                                                           <  3:752/141‑1 (MQ=255)
                                                                        aaatTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGatat                                                           <  1:768720/141‑1 (MQ=255)
                                                                          atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                         <  1:48734/141‑1 (MQ=255)
                                                                          atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                         <  3:131545/141‑1 (MQ=255)
                                                                          atTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTg                                                         >  2:733269/1‑141 (MQ=255)
                                                                           ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                        <  4:431752/141‑1 (MQ=255)
                                                                           ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                        >  1:573272/1‑141 (MQ=255)
                                                                           ttATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGt                                                        >  1:991631/1‑141 (MQ=255)
                                                                             atatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaa                                                      <  3:131698/141‑1 (MQ=255)
                                                                              tatTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaa                                                     <  1:251540/141‑1 (MQ=255)
                                                                               atTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTaaaa                                                    >  4:136575/1‑141 (MQ=255)
                                                                                 tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                                  >  3:454555/1‑141 (MQ=255)
                                                                                 tGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATc                                                  <  1:269087/141‑1 (MQ=255)
                                                                                    gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                               >  3:358635/1‑141 (MQ=255)
                                                                                    gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                               >  1:66578/1‑141 (MQ=255)
                                                                                    gTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCtaa                                               >  1:862810/1‑141 (MQ=255)
                                                                                         aCAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGt                                          <  1:667591/141‑1 (MQ=255)
                                                                                            aGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGt                                       <  2:233195/141‑1 (MQ=255)
                                                                                              cAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaa                                     <  2:737375/141‑1 (MQ=255)
                                                                                               aaaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaat                                    <  2:728635/141‑1 (MQ=255)
                                                                                               aaaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaat                                    <  1:351535/141‑1 (MQ=255)
                                                                                                tatgtCATACTTATGTATGGATTGGGTAATTGTAGAAAAATAAAATTATTTCAAATAGAGAAAGAGATTGTAATCATTTTATAGTTAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                   <  2:409072/137‑1 (MQ=255)
                                                                                                aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                   >  4:268835/1‑141 (MQ=255)
                                                                                                aaGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                   <  2:1082117/141‑1 (MQ=255)
                                                                                                aaGTTCATGCTTATGTTTGTAGGGCGTCATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATAGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaata                                   >  2:979254/1‑141 (MQ=255)
                                                                                                 aGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGtaataa                                  <  2:469343/141‑1 (MQ=255)
                                                                                                    tCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAttt                               <  2:71694/141‑1 (MQ=255)
                                                                                                     cATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAtttt                              >  1:906408/1‑141 (MQ=255)
                                                                                                     cATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAAtttt                              >  1:638415/1‑141 (MQ=255)
                                                                                                      aTGCTTATGTTTGTAGGGCGTTATTGTTTGACAAAATATTTATTTCCAATAGAGAAAGGGTATGTAATCATGTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                             <  2:300226/141‑1 (MQ=255)
                                                                                                      aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTTGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                             >  2:518177/1‑141 (MQ=255)
                                                                                                      aTGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTa                             >  1:764522/1‑141 (MQ=255)
                                                                                                       tGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATCATTTTaa                            >  2:314314/1‑141 (MQ=255)
                                                                                                             tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                      <  2:331611/141‑1 (MQ=255)
                                                                                                             tGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTa                      >  4:403266/1‑141 (MQ=255)
                                                                                                               tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                    >  1:853895/1‑141 (MQ=255)
                                                                                                               tttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAt                    <  1:1033491/141‑1 (MQ=255)
                                                                                                                ttGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAAtt                   <  2:467546/141‑1 (MQ=255)
                                                                                                                ttGTAGGGCGTTATTGTTGAAGAATAAAATTATTTCCAATAGAGAAAGGGTTTGTAATCATATTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATATTTTTAAGGGGTAAtt                   <  4:53108/141‑1 (MQ=255)
                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  1:30043/141‑1 (MQ=255)
                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  2:518105/141‑1 (MQ=255)
                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  4:373457/141‑1 (MQ=255)
                                                                                                                  gTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtat                 <  1:445895/141‑1 (MQ=255)
                                                                                                                   tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                >  2:1070771/1‑141 (MQ=255)
                                                                                                                   tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                >  2:960458/1‑141 (MQ=255)
                                                                                                                   tAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATtata                >  3:258238/1‑141 (MQ=255)
                                                                                                                     gggCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATaaa              >  1:154024/1‑141 (MQ=255)
                                                                                                                       gCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAAt            >  2:65865/1‑141 (MQ=255)
                                                                                                                         gTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAAttt          <  3:221314/141‑1 (MQ=255)
                                                                                                                            aTTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTtgat       <  2:629270/141‑1 (MQ=255)
                                                                                                                                 tgGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATAc  <  3:308011/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
TCAAAGATGGAGAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAATAAATTATATTGCGTCCTACAAGCAAAGTTCATGCTTATGTTTGTAGGGCGTTATTGTTGGAGAATAAAATTATTTCCAATAGAGAAAGGGATTGTAATCATTTTATAGTGAAATATTATGAAATTGTAATAATTTAGATATTGTAAAATCTAATAAGTTGTAATAATTTTAAGGGGTAATTATAAAATTTGATGATAC  >  USA300TCH1516_ALE/491169‑491438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: