Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 614,094 T→C 9.4% S802S (AGT→AGC sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE614,0940TC 9.4% 314.5 / 2.4 129S802S (AGT→AGCsdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base T (59/51);  new base C (13/2);  total (75/54)
Fisher's exact test for biased strand distribution p-value = 2.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

CAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGA  >  USA300TCH1516_ALE/613955‑614234
                                                                                                                                           |                                                                                                                                            
cagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                                                                                                                             >  3:375535/1‑141 (MQ=255)
cagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                                                                                                                             <  1:230684/141‑1 (MQ=255)
 agattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTga                                                                                                                                            <  2:1068299/141‑1 (MQ=255)
    ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                                                                                                                         <  3:53743/141‑1 (MQ=255)
    ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                                                                                                                         >  3:194941/1‑141 (MQ=255)
    ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACAGAgattc                                                                                                                                         >  2:81181/1‑141 (MQ=255)
    ttcagaCACCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                                                                                                                         <  2:69052/141‑1 (MQ=255)
     tcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattca                                                                                                                                        >  1:245543/1‑141 (MQ=255)
      cagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcag                                                                                                                                       >  1:206548/1‑141 (MQ=255)
      cagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGAgattcag                                                                                                                                       >  2:934838/1‑141 (MQ=255)
        gaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagat                                                                                                                                     >  1:21833/1‑141 (MQ=255)
          cAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                                                                                                                                   >  3:258053/1‑141 (MQ=255)
            gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGattcagnttcag                                                                                                                                 >  1:305175/1‑141 (MQ=255)
                   agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGa                                                                                                                          <  1:533264/141‑1 (MQ=255)
                   agactcagacAGCGATCCAGGGTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGa                                                                                                                          <  1:826237/141‑1 (MQ=255)
                    gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGAt                                                                                                                         <  1:199903/141‑1 (MQ=255)
                    gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACTCAGAt                                                                                                                         >  2:937328/1‑141 (MQ=255)
                    gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACACAGAt                                                                                                                         >  2:685980/1‑141 (MQ=255)
                       tcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGc                                                                                                                      <  1:192038/141‑1 (MQ=255)
                          gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                                                                                                   <  4:354458/141‑1 (MQ=255)
                          gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                                                                                                   <  1:405584/141‑1 (MQ=255)
                          gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTCAGACACAGATACCGAc                                                                                                                   >  2:96932/1‑141 (MQ=255)
                          gacAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                                                                                                   <  2:592486/141‑1 (MQ=255)
                               cGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATCGCGCTTCGGACTCAGACAGTGATTCAGATTCAGACGCAGCTAGCGACTCAGa                                                                                                              >  1:980228/1‑141 (MQ=255)
                               cGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGa                                                                                                              <  2:995693/141‑1 (MQ=255)
                                     aGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGa                                                                                                        <  3:314051/141‑1 (MQ=255)
                                      gACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGAc                                                                                                       >  1:65276/1‑141 (MQ=255)
                                        cTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAg                                                                                                     >  1:640141/1‑141 (MQ=255)
                                        cTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAg                                                                                                     <  1:927234/141‑1 (MQ=255)
                                         tCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGc                                                                                                    <  3:393489/141‑1 (MQ=255)
                                              tAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtc                                                                                               <  1:840229/141‑1 (MQ=255)
                                              caGCCACCCAGACTCAGACAGCGACGCAGATTCGGATCGCGACTCACGCTCAGCTGGCGATTCAGATGCAGATAGCGATTCGGACTCAGACTGCGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtc                                                                                               <  2:328636/140‑1 (MQ=18)
                                               aGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtca                                                                                              >  1:411571/1‑141 (MQ=255)
                                                  gactcagactcaGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagac                                                                                           >  1:960552/1‑141 (MQ=255)
                                                  gactcagactcaGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagac                                                                                           >  3:455878/1‑141 (MQ=255)
                                                    ctcagactcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTTAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactc                                                                                         <  2:573728/141‑1 (MQ=255)
                                                       agactcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactcaga                                                                                      <  1:489958/141‑1 (MQ=255)
                                                         actcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACa                                                                                    >  1:770033/1‑141 (MQ=255)
                                                          ctcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAg                                                                                   >  1:557585/1‑141 (MQ=255)
                                                          ctcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAg                                                                                   <  1:974324/141‑1 (MQ=255)
                                                             agaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCga                                                                                <  1:611550/141‑1 (MQ=255)
                                                             agaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCga                                                                                <  2:106052/141‑1 (MQ=255)
                                                             agaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACACAGATAGCGATTCAGATTCCGACAACGATTCAGCCTCAGACAgaga                                                                                >  2:514774/1‑141 (MQ=18)
                                                             agaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCGGACTCAGACAGTGATTCAGATTCAGACACAGACAGAGACTCAGATACCGAAAGCGATTCAGACTCAGACAgaga                                                                                >  2:514781/1‑141 (MQ=18)
                                                                 aGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactca                                                                            >  3:205250/1‑141 (MQ=255)
                                                                    gACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACACAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcagac                                                                         >  2:1046156/1‑141 (MQ=255)
                                                                     aCTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATACTGACAGCGATTCAGACACAGACAGCgactcagaca                                                                        >  2:253723/1‑140 (MQ=255)
                                                                        cAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                     <  1:713122/141‑1 (MQ=255)
                                                                        cAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACACAGATACCGACTCAGATACTGACAGCGATTCAGACACCGACAGCGactcagactcag                                                                     >  4:407339/1‑141 (MQ=25)
                                                                         aGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGActcagactcaga                                                                    <  3:105270/141‑1 (MQ=255)
                                                                          gATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACtcagactcagac                                                                   >  1:534390/1‑141 (MQ=255)
                                                                          aatgcGGACACCGACTCACACGCAGAGAGAGATTCAGATTCAGATTGCGATTCGGACTCAGACTGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACtcagactcagac                                                                   <  2:915107/137‑1 (MQ=21)
                                                                            ttCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAg                                                                 >  3:218288/1‑141 (MQ=255)
                                                                             tCGGATAGCGACTCAGACTCAGATAGCGACGCGGGGTCAGATAGCGACTCGGACTCAGACAGCGACTCAGATTCAGACTCAGATACCGACTCAGATCCTGACAGCGATTCAGACTCAGACAGCGACCCAGACTCAGACAGt                                                                >  2:323039/1‑141 (MQ=21)
                                                                              cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAgcg                                                               >  1:176973/1‑139 (MQ=255)
                                                                              cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTg                                                               <  1:737040/141‑1 (MQ=255)
                                                                              cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACACAGACAGCGACTCAGACTCAGACAGTg                                                               >  1:458608/1‑141 (MQ=255)
                                                                              cGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGATTCAGATTCAGACACCGACACAGATTCAGATACTGATTCCGATTCAGACTCAGACACCGACACAGACacagacagcg                                                               >  2:66206/1‑139 (MQ=11)
                                                                               ggATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCCGATAGCGACTCAGACTCAGACAGCGActcagattcagagcgcga                                                              >  2:273426/1‑135 (MQ=18)
                                                                               ggATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACTCAGATAGAGACTCAGATACAGATAGCGACTCAGACTCAGAGAGAGAGTCAGATACAgagagaga                                                              >  2:520922/1‑141 (MQ=17)
                                                                                     cGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACCCACACAGTgattcaga                                                        >  1:875736/1‑141 (MQ=255)
                                                                                      gactcagactcaGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGACAGTgattcagat                                                       <  2:494147/141‑1 (MQ=255)
                                                                                      gactcagactcaGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattcagat                                                       <  1:643700/141‑1 (MQ=255)
                                                                                        ctcagactcagaTAGCGATTCAGATTCAGATTGCGATTCGGACTCAGACAGAGACTCAGATTCAGACTCAGATAGCGACTCAGACTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattcagattc                                                     <  2:226333/141‑1 (MQ=255)
                                                                                                 agaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACCCAGACAGCGACTCAGACACAGACAGTGATTCAGATGCAGACACCGa                                            >  2:1046664/1‑141 (MQ=255)
                                                                                                  gaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGAc                                           >  4:31360/1‑141 (MQ=255)
                                                                                                   aTACCGATTCAGATAAAGATTCCGATTCCGACAAAGACTCTGATTCAGATGCAGATTCAGATAGCGACTCAGACTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACt                                          <  2:818825/141‑1 (MQ=17)
                                                                                                      gagatgcagattcaGATAGCGAGGCTGACACAGACTATGATTCCGATTCAGACTCAGATAGCGACTCAGATTCTGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGattcgg                                       <  4:28313/139‑6 (MQ=14)
                                                                                                        gattcagattcaGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                     >  1:404393/1‑141 (MQ=255)
                                                                                                            cagattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                 >  2:468839/1‑141 (MQ=255)
                                                                                                             cgattcagatAGCGATTCGGACTGAGCCGGCGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcaga                                <  1:1048816/140‑1 (MQ=255)
                                                                                                             agattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGATTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcaga                                <  1:549146/141‑1 (MQ=255)
                                                                                                                ttcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGa                                                                                                        >  4:382919/1‑66 (MQ=255)
                                                                                                                ttcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGa                                                                                                        <  3:382919/66‑1 (MQ=255)
                                                                                                                ttcagatAGCGATTCGGACTCAGACAGTGATACAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAGTGAGACTCAGACAGCGACTCACACTGAGACAGGTATCCATATTCAGACAGCGACTCAGATtcagatag                             >  4:405169/1‑141 (MQ=18)
                                                                                                                  cagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcg                           >  2:608047/1‑141 (MQ=255)
                                                                                                                   agatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcga                          >  1:838028/1‑141 (MQ=255)
                                                                                                                    gatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                         >  2:963320/1‑141 (MQ=255)
                                                                                                                    gatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                         >  1:509480/1‑141 (MQ=255)
                                                                                                                    gatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                         <  3:304014/141‑1 (MQ=255)
                                                                                                                     ataacGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgact                        >  2:391771/5‑141 (MQ=255)
                                                                                                                        gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcag                     >  4:226539/1‑141 (MQ=255)
                                                                                                                        gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcag                     >  2:471893/1‑141 (MQ=255)
                                                                                                                        gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATGCAGACTCAGACAGCGACTCAGGCTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcag                     >  1:919447/1‑141 (MQ=255)
                                                                                                                         cGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcaga                    >  4:32141/1‑141 (MQ=255)
                                                                                                                         cGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcaga                    <  4:355799/141‑1 (MQ=255)
                                                                                                                          gATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagac                   >  2:364597/1‑141 (MQ=255)
                                                                                                                          gATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagac                   >  2:804464/1‑141 (MQ=255)
                                                                                                                            ttCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactc                 <  3:158810/141‑1 (MQ=255)
                                                                                                                             tCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactca                >  2:468511/1‑141 (MQ=255)
                                                                                                                              cGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcag               >  1:143754/1‑141 (MQ=255)
                                                                                                                              cGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcag               >  3:56690/1‑141 (MQ=255)
                                                                                                                              cGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcag               >  3:207687/1‑141 (MQ=255)
                                                                                                                               ggACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcaga              <  3:393992/141‑1 (MQ=255)
                                                                                                                                gACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagat             >  1:315195/1‑141 (MQ=255)
                                                                                                                                  cTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatag           >  1:153754/1‑141 (MQ=255)
                                                                                                                                  cTCAGACAGGGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATGCAGACAGCGACTCAGATacagaaagcgactcagactcagataa           >  4:412985/1‑140 (MQ=18)
                                                                                                                                   tCAGACTCTGATTAAGACTCAGACTCAGATAGAGACTCAGACTCTGACGGAGATTCAGACTCAGACAGCGACTCAGACTCTGACAGTGATTCAGATTCAGATAGCGACTCAGATtcagatagcgactcagactcagatagc          <  2:97803/141‑1 (MQ=14)
                                                                                                                                      gACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgac       <  1:326607/141‑1 (MQ=255)
                                                                                                                                          gTGATTCAGATTCAGACTCAGATAGCGACTCCGATACTAACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcag   >  1:29901/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGA  >  USA300TCH1516_ALE/613955‑614234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: