Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,483 T→C 5.4% D1195D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,4830TC 5.4% 361.8 / 4.8 134D1195D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (66/60);  new base C (4/4);  total (70/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTC  >  USA300TCH1516_ALE/618345‑618623
                                                                                                                                          |                                                                                                                                            
tAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAg                                                                                                                                            >  1:592091/1‑141 (MQ=255)
  gCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACACAGATTCAGACAGCGACTCAGACTCAGATAGTg                                                                                                                                          >  4:344268/1‑141 (MQ=255)
   cGATTCAGATTCAGACAGCCATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGa                                                                                                                                         <  1:700993/141‑1 (MQ=255)
         agatgcagaCAGAGATTCAGATTAAGATAACGATTCAGATCCAGACAAAGACTCAGATAGAGATGCAGAATCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGa                                                                                                                                   <  2:510207/141‑1 (MQ=18)
            tgctgaCATCGATTCAGAATAAGATAGCGATTCAGAAGCAGACAGAGGCTCAGACAGAGAATAAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTc                                                                                                                                <  2:116226/137‑1 (MQ=18)
             tcagaCAGAGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCa                                                                                                                               <  1:372472/141‑1 (MQ=255)
              cagaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAg                                                                                                                              >  1:155484/1‑141 (MQ=255)
              cagaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAg                                                                                                                              >  1:484549/1‑141 (MQ=255)
              cagaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAg                                                                                                                              >  2:1048931/1‑141 (MQ=255)
               agaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGa                                                                                                                             >  2:493575/1‑141 (MQ=255)
               agaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGa                                                                                                                             <  1:408962/141‑1 (MQ=255)
                gaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGAt                                                                                                                            <  1:672798/141‑1 (MQ=255)
                gaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGAt                                                                                                                            >  2:673135/1‑141 (MQ=255)
                gaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGAt                                                                                                                            >  2:672798/1‑141 (MQ=255)
                  cAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg                                                                                                                          <  1:777987/141‑1 (MQ=14)
                     cGATTCAGAGTCAGACAGCGACTCCGAGTCAGACAGAGGCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGa                                                                                                                       <  2:648720/141‑1 (MQ=17)
                      gattcagattcaGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGAc                                                                                                                      <  1:511685/141‑1 (MQ=255)
                      gaatcagagtcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATGCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGAc                                                                                                                      <  4:296615/138‑1 (MQ=18)
                      aattcagcttcagATAACGATTCAGATTCAGATAATGAATCAGACAGTGATTAAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGAc                                                                                                                      <  4:57797/140‑1 (MQ=17)
                       attcagattcagATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACt                                                                                                                     >  2:232773/1‑141 (MQ=255)
                        ttcagattcagaTAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATCGCGACTc                                                                                                                    >  4:207216/1‑141 (MQ=255)
                          cagattcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAg                                                                                                                  <  1:790740/141‑1 (MQ=255)
                          cagattcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAg                                                                                                                  >  1:1001628/1‑141 (MQ=255)
                           agattcagatAGAGATTCAGATTCAGACTGTGACTCAGACACAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa                                                                                                                 <  2:787504/141‑1 (MQ=17)
                            gattcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACTGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACACAGAt                                                                                                                >  2:1050585/1‑141 (MQ=255)
                             attcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAtt                                                                                                               >  1:923020/1‑141 (MQ=255)
                                cagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcag                                                                                                            >  1:260946/1‑141 (MQ=255)
                                cagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAGTCAGACAGCGACACAGACACAGACAGTGACACCGACTGACACAACAACTCAGAAtcgg                                                                                                            >  2:384546/1‑139 (MQ=17)
                                 agatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGGGATTCAGACTCAGATAGCGACTCAGATtcaga                                                                                                           >  2:14814/1‑141 (MQ=255)
                                  gatacagattcaaattcaGATAGTGACTCAGATGGTGATTGAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGTGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagac                                                                                                          <  2:874614/135‑1 (MQ=17)
                                  gatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagac                                                                                                          <  3:325955/141‑1 (MQ=255)
                                        gattcagattcaGACAGAGATTCAGATAGTGATGCAGATTCAGATAGCGACGCAGATTCAGACAGAGACTAAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgac                                                                                                    <  2:239383/141‑1 (MQ=17)
                                        gattcagattcaGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgac                                                                                                    <  1:832652/141‑1 (MQ=255)
                                          ttcagattcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactc                                                                                                  >  1:693686/1‑141 (MQ=255)
                                          ttcagattcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactc                                                                                                  >  1:1053140/1‑141 (MQ=255)
                                              gattcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagac                                                                                              <  1:966576/141‑1 (MQ=255)
                                                ttcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagactc                                                                                            >  1:229790/1‑141 (MQ=255)
                                                ttcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGAGtcagacagggatcaagactc                                                                                            >  4:427619/1‑141 (MQ=14)
                                                   agacagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagactcaga                                                                                         >  1:949607/1‑141 (MQ=255)
                                                    gacagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcggacagcgattcagactcagat                                                                                        >  2:132713/1‑140 (MQ=255)
                                                    gacagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagaa                                                                                        >  2:89018/1‑140 (MQ=255)
                                                    aacagtGAATCAGAGAGCGATTCAGAATCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGAATCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagactcagac                                                                                        <  4:43532/140‑1 (MQ=14)
                                                      tcgagaCACAGATAGCGATTCAGACTCAGAGAGCGACTCAGATGCAGACACTGACACAGATTCAGACAGTGACTCAGACTCAGATAGTGATTCAGACTCAGATGGCGACTCAGCTtcagacagcgactcagactcagacag                                                                                      <  2:66793/139‑1 (MQ=14)
                                                      cagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagactcagacag                                                                                      <  2:701808/141‑1 (MQ=255)
                                                      cagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagactcagacag                                                                                      >  3:6420/1‑141 (MQ=255)
                                                            cTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCCGACCGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactcagactcagacagcgactc                                                                                <  1:613787/141‑1 (MQ=255)
                                                            cTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATACCGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagagagtgagactcagacagcgactc                                                                                >  2:98832/1‑141 (MQ=14)
                                                                   ttggacacAGACTCAGAGAGCGACTCAGATTCAGAGACTGATGAAGATTCAGACAAGGACTATGACTCAGATAGTGAATCAGACTCAGATAGCGACTCAGATTCAgacagcgactcagactcagacagcgactcagactca                                                                         <  4:122869/134‑1 (MQ=14)
                                                                   cgTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAgacagcgactcagactcagacagcgactcagactca                                                                         <  1:971393/140‑1 (MQ=255)
                                                                     agATTCAGACTCAGATAGCGGCTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAcagcgactcagactcagacagcgactcagactcaga                                                                       <  1:1042987/140‑1 (MQ=255)
                                                                           aGACTCAGATAGCGACTCAGATTCAGACTGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGAGATGCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGa                                                                 <  2:28995/141‑1 (MQ=255)
                                                                            gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGAc                                                                <  1:326099/141‑1 (MQ=255)
                                                                              cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCCGACAGCGACTCAGAATCCGCCAGCGACTAAGACTCAGATAGTGACtc                                                              >  1:864195/1‑141 (MQ=17)
                                                                              cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtc                                                              <  2:746199/141‑1 (MQ=255)
                                                                              cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCagactcagatagcgactcagactcagatagcgactc                                                              >  2:942569/1‑141 (MQ=255)
                                                                                cAGATCGCGACACAGATTCAGACCGCGACGCAGATTCAGAAAGAGACTCAGACTCAGACAATGACGCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcag                                                            <  4:223585/141‑1 (MQ=14)
                                                                                 aGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcaga                                                           >  1:519819/1‑141 (MQ=255)
                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGTCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                        <  1:483336/141‑1 (MQ=255)
                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                        >  1:690434/1‑141 (MQ=255)
                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                        >  3:299687/1‑141 (MQ=255)
                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                        >  3:299553/1‑141 (MQ=255)
                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACACAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGGGACtcagagtc                                                        >  1:457724/1‑141 (MQ=255)
                                                                                    tAGCGACGTAGATTCAGACAACGACTCCGATTCAGACAGAGACACAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                        <  2:184080/141‑1 (MQ=17)
                                                                                     agcgactcagattcagacagcgactcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGa                                                                                                                             <  2:644919/71‑1 (MQ=255)
                                                                                     agcgactcagattcagacagcgactcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGa                                                                                                                             >  1:644919/1‑71 (MQ=255)
                                                                                     agcgactcagattcagacagcgactcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattca                                                       <  2:241889/141‑1 (MQ=255)
                                                                                          ctcagattcagacagcgactcagattcagacagcgaCTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAg                                                  >  1:447309/1‑141 (MQ=255)
                                                                                              gattcagacagtgattcagattcagacggcgacccaGACTCAGACGGTGAGTAAGACTCAGATAGCGACTCAGATTCAGACGGCGACTCAGACTCAGACTGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAc                                              <  2:90517/141‑1 (MQ=17)
                                                                                                 tcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCa                                           >  3:280409/1‑141 (MQ=255)
                                                                                                 tcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACGCAGACTCAGATAGTGACTCAGACTCAGATAGCggcaca                                           >  2:502841/1‑136 (MQ=255)
                                                                                                  cagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAg                                          >  1:342632/1‑141 (MQ=255)
                                                                                                   agacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGa                                         >  3:178706/1‑141 (MQ=255)
                                                                                                   agacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGa                                         <  2:988933/141‑1 (MQ=255)
                                                                                                      cagcgactcagattcagacagcgactcagaCTCAGATAg                                                                                                                                            >  2:788256/1‑39 (MQ=40)
                                                                                                      cagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTc                                      >  1:574076/1‑141 (MQ=255)
                                                                                                         cgactcagattccgacagggactccgaCTCAGATAGTGATTCAGACTCAGATAGCGACGCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGTCTCTGATAGTGACTCAGATTCAGATAGCGACTCAGATTCTGa                                   <  2:792066/141‑1 (MQ=17)
                                                                                                         cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                   <  2:159232/141‑1 (MQ=255)
                                                                                                          gattcagactcagacagcgactcagaTTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                  >  1:923375/4‑141 (MQ=1)
                                                                                                            ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGAc                                                                                                                      >  3:189472/1‑55 (MQ=38)
                                                                                                               agattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa                             <  1:485297/141‑1 (MQ=255)
                                                                                                               agactcagacagcgactcagaTTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGa                             <  1:174937/137‑1 (MQ=11)
                                                                                                                gattcagacagtgattcagaCTCAGACAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                            >  3:74896/1‑141 (MQ=11)
                                                                                                                gattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                            >  3:364280/1‑141 (MQ=255)
                                                                                                                gattcagacagcgactcagaCTCAGATAGTGATTCAGACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGAGAGCGACTCAGACTCAGACAGCGACTCCGACACAGATAGCGACTCAGACTCCGACAGCGag                            >  2:955888/1‑140 (MQ=14)
                                                                                                                gattcagacagagattaagaCTAAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                            <  2:956975/141‑1 (MQ=255)
                                                                                                                 attcagacagagactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAtt                           <  4:427375/141‑1 (MQ=255)
                                                                                                                  ttcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                          >  4:31361/1‑141 (MQ=255)
                                                                                                                    cagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGATTCAGATAGCGACTCAGAGTCCGACAGCGATTCAg                        >  2:28127/1‑141 (MQ=255)
                                                                                                                    cagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGAtacgg                        >  2:178909/1‑137 (MQ=14)
                                                                                                                         agcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGAGACTCAGACCCAGATAGCGACACAGAGTCGGATAGCGAGTCAGACTCa                   >  2:380099/1‑141 (MQ=14)
                                                                                                                                 agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa           <  3:437340/141‑1 (MQ=255)
                                                                                                                                 agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa           >  1:4001/1‑141 (MQ=255)
                                                                                                                                 agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACACAGATAGCGa           >  2:231138/1‑141 (MQ=255)
                                                                                                                                  gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc          <  1:672749/141‑1 (MQ=255)
                                                                                                                                    ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCCGATAGCGACGCGGACTCGGAAACCGATTCATATGCTGATAGCGACtc        >  2:430144/1‑141 (MQ=14)
                                                                                                                                     tcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca       >  1:446687/1‑141 (MQ=255)
                                                                                                                                      cagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACCGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag      <  4:262341/141‑1 (MQ=255)
                                                                                                                                       agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACCGCGACTCAGACTCAGCCAGCGACTCAGACTCAGATAGTGAATCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga     <  3:130460/141‑1 (MQ=255)
                                                                                                                                        gaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat    <  3:60636/141‑1 (MQ=255)
                                                                                                                                          tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattc  <  2:739173/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTC  >  USA300TCH1516_ALE/618345‑618623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: