Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,750 G→A 6.4% S1284S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,7500GA 6.4% 337.7 / 2.7 126S1284S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (58/55);  new base A (5/5);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAAT  >  USA300TCH1516_ALE/618614‑618890
                                                                                                                                        |                                                                                                                                            
ccTCAGATTCTGACACCGTTTCGGACTCGTATACCGACTCAGATTCAGATAGTGACTCAGACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATa                                                                                                                                          <  4:299687/140‑1 (MQ=17)
ccTCAGATTCAGATACCGATTCGGACTCAGATACCCCCTCAGACTCAGATAGTGTCTCAGACTCACATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATa                                                                                                                                          <  4:299553/140‑1 (MQ=17)
aCTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATa                                                                                                                                          <  1:384546/141‑1 (MQ=255)
 cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGATTCAAATACTGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAg                                                                                                                                         <  3:115788/141‑1 (MQ=255)
  tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGTGACTCAGAGTCAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAgg                                                                                                                                        >  2:746485/1‑140 (MQ=14)
    agtttcagatAGCGATTCAGACTCAGTGAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGa                                                                                                                                      <  1:430144/138‑1 (MQ=255)
      attcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGAtt                                                                                                                                    >  2:163426/1‑141 (MQ=255)
       ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGGTTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                                                   >  1:102461/1‑141 (MQ=255)
       ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                                                   >  1:145195/1‑141 (MQ=255)
       ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                                                   <  3:143442/141‑1 (MQ=255)
       ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                                                   >  3:36708/1‑141 (MQ=255)
       ttcagagAGCGAGTCGGACTCATATAGCGACGCAGACTCTGATACTTACTCATACGCATATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                                                   <  1:98832/141‑1 (MQ=18)
        tcagatAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATCGCGACACAGATTCAGACAGCGACTCAGATTCCGATTGCGACTCTGTTCGAGACAGCCACACAGACCCAGATAGGGacaca                                                                                                                                  >  4:234851/1‑137 (MQ=14)
        tcagatAGCGATTCGGAATCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCCGATAGCGACTCAGATTCAGACAGCGACTGAGACTCGGATAGTGATTCa                                                                                                                                  >  4:225351/1‑141 (MQ=255)
         cagatAGCGATTCGGACTCAGATGGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg                                                                                                                                 <  3:368720/141‑1 (MQ=255)
          agatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGa                                                                                                                                <  1:65815/141‑1 (MQ=255)
           gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAc                                                                                                                               <  1:86090/141‑1 (MQ=255)
           gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAc                                                                                                                               >  1:947521/1‑141 (MQ=255)
             tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                                                                             <  1:151996/141‑1 (MQ=255)
             tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                                                                             >  1:227719/1‑141 (MQ=255)
             tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTAGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                                                                             >  3:243026/1‑141 (MQ=255)
              accGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCa                                                                                                                            <  2:229790/139‑1 (MQ=255)
               gCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAg                                                                                                                           >  3:295887/1‑141 (MQ=255)
                cGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGa                                                                                                                          >  1:577893/1‑141 (MQ=255)
                   ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                                                                                                                       >  3:418960/1‑141 (MQ=255)
                      ggACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCga                                                                                                                    <  3:76197/141‑1 (MQ=255)
                       gACTCAGCTATCCACTCAGATTCAGATACTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgat                                                                                                                   <  2:949607/141‑2 (MQ=17)
                        aCTCAGATAGCGACTCAGATTCAGATAGTGGTTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGAGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCcact                                                                                                                  >  4:107203/1‑141 (MQ=255)
                          tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAACgactca                                                                                                                >  4:369124/1‑141 (MQ=255)
                           cAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcag                                                                                                               >  2:490707/1‑141 (MQ=255)
                                   gACTCAGCTTCAGATAGTGATTCAGACTCAGATAGCGACTCAGGTTCAGAGAGCGACGCAGATTCGGGTAGCGACTCAGATTCAGACAGCGACTCAGACGCGGATAGTGACTCAGACTCAGATAGCGACTCGCACTCAGAt                                                                                                       >  4:146772/1‑141 (MQ=17)
                                   gACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGAt                                                                                                       >  3:431927/1‑141 (MQ=255)
                                   gACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGAt                                                                                                       >  3:338229/1‑141 (MQ=255)
                                   gACTCAGATTCAGATAGTGAATCAGACTCATATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGAt                                                                                                       <  2:1027116/141‑1 (MQ=255)
                                      tcagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGc                                                                                                    <  1:116085/141‑1 (MQ=255)
                                      tcagagtcagatAGTGATTCAGACTCAGATAGCGATTCAGACTCAGACAGGGACTCAGACGAGGTTAGTGACAGTGATTGGGGCTGAGACGTAGACTCGGCTGGTGACTCAGACTCAGATAGTGACTCAGACTCAGATAGc                                                                                                    <  3:7201/141‑1 (MQ=12)
                                        gtattcacgtACTGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCga                                                                                                  <  2:282072/139‑1 (MQ=17)
                                        atattcagatAGCGAATCAGACTCGTATGGCTTGGCGGATTCAGACAGCCGCTTGTTTTCGAAGAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCga                                                                                                  <  1:593629/139‑1 (MQ=9)
                                        agattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCga                                                                                                  <  1:1023229/141‑1 (MQ=255)
                                        agattcagatAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCga                                                                                                  <  2:403140/141‑1 (MQ=255)
                                               gatAGTGATTCAGACTCAGATAGCGACTCAGATTCCGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGAgattcagat                                                                                           >  1:503473/1‑141 (MQ=255)
                                                        tCAGCCGCAGATATCGACTCCGCTACATACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGc                                                                                  <  2:833583/141‑1 (MQ=14)
                                                        tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAga                                                                                  >  4:55986/1‑140 (MQ=255)
                                                         cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCg                                                                                 >  1:739405/1‑141 (MQ=255)
                                                          aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGCCTCAGATTCAGACACCGACTCACACTCGGACAGCGATTCAGACTCAGATACCGACTCAGACCAACATAGCAAAACAGATGCAGATAGCga                                                                                >  4:72050/1‑141 (MQ=17)
                                                           gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgac                                                                               >  4:34446/1‑141 (MQ=255)
                                                           gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgac                                                                               <  3:336992/141‑1 (MQ=255)
                                                             cttaGATAGCGACTCAGATTCAGACAGGGACATAGACTCGGATAGTGACTCAGATACAGATAGCGACCCTGACTCGGAGAGTGATTCAGACTCAGATAGCGACTAAGACGCAGATAGCAATTCAGATACAGATAGAtactc                                                                             >  4:280813/4‑141 (MQ=14)
                                                             cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGGCTCAGATAGCGACTCAGTCTCGGATAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGAAAGCGAGTCAGACTCAGGTAGGgacta                                                                             >  2:152574/1‑140 (MQ=11)
                                                             cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGGCTCAGACTCAGATAGCGATTCAGATTCAGATAGCggctc                                                                             >  3:1209/1‑141 (MQ=255)
                                                                aGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcaga                                                                          >  3:319551/1‑141 (MQ=255)
                                                                aGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcaga                                                                          >  1:926999/1‑141 (MQ=255)
                                                                      cGACTCAGATTCAGACAGCGACTCCGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGACAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGActcagactcaga                                                                    <  2:643348/141‑1 (MQ=255)
                                                                      cGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGActcagactcaga                                                                    <  2:758265/141‑1 (MQ=255)
                                                                         cTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAg                                                                 <  1:347091/141‑1 (MQ=14)
                                                                         cTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAg                                                                 <  1:967019/141‑1 (MQ=14)
                                                                              actCAGACACCGTCTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAtt                                                            <  2:574043/139‑1 (MQ=14)
                                                                              actCACACAGCGCCTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAtt                                                            <  2:574076/139‑1 (MQ=255)
                                                                               ttCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtc                                                           >  1:89330/1‑141 (MQ=255)
                                                                                tCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtca                                                          >  1:1002321/1‑141 (MQ=255)
                                                                                  aGACAGCGGCTCAGATGCGGATAGCGACTCAGATTCAGACGGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcaga                                                        <  1:217182/141‑1 (MQ=255)
                                                                                   gACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagac                                                       >  2:199775/1‑141 (MQ=255)
                                                                                   gACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagac                                                       >  2:497423/1‑141 (MQ=255)
                                                                                     cAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactc                                                     >  1:1022387/1‑141 (MQ=255)
                                                                                     cAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactc                                                     <  1:746485/141‑1 (MQ=255)
                                                                                       gtgACTCAGATTCCGATAGTGACTCGGATTCAGCGAGTGATTCAGATTCAGATAGCGATTCAGAATCAGATAGTGACTCAGACTCAGACCGTGATTCAGATTCAGATAGTGACTCAGACTCAGACAGCGATtcagaatcag                                                   >  2:531979/3‑141 (MQ=2)
                                                                                        cGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagactcaga                                                  <  1:325629/141‑1 (MQ=255)
                                                                                          aCTCAGATTCGGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACa                                                <  2:260946/141‑1 (MQ=14)
                                                                                          aCTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTAAGATACAAATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACa                                                >  1:19614/1‑141 (MQ=255)
                                                                                           cTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAg                                               >  1:339885/1‑141 (MQ=255)
                                                                                              aGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCga                                            >  2:780828/1‑141 (MQ=255)
                                                                                               gATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgac                                           <  1:942569/141‑1 (MQ=255)
                                                                                                actcagATAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgact                                          <  4:230637/136‑1 (MQ=255)
                                                                                                  tCGGATAGCGACTCAGATACAGAAAGCGACTCAGACACGGATAGTCATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactca                                        <  2:312415/141‑1 (MQ=255)
                                                                                                       tAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAg                                                                                                                                         <  1:788256/39‑1 (MQ=255)
                                                                                                          cGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGActcagactcaga                                >  3:259521/1‑141 (MQ=255)
                                                                                                          cGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGActcagactcaga                                >  3:143624/1‑141 (MQ=255)
                                                                                                             cTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgac                                                                                                                   <  4:189472/55‑1 (MQ=38)
                                                                                                                  cttCAGACAGCGACTCAGCCTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTa                        <  2:4001/140‑1 (MQ=255)
                                                                                                                  actccgACAACGACTCAGTCTCCGATAGCGAATCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTa                        <  4:280409/136‑1 (MQ=17)
                                                                                                                  aTTCAGACAGCGACTCAGACTCTGATAGTGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTa                        <  1:502841/141‑1 (MQ=255)
                                                                                                                    tCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAg                      >  2:390988/1‑141 (MQ=255)
                                                                                                                    tCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAg                      >  2:736954/1‑141 (MQ=255)
                                                                                                                     cAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGc                     >  3:444776/1‑141 (MQ=255)
                                                                                                                      aGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGca                    <  1:178909/141‑1 (MQ=255)
                                                                                                                       gACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcac                   >  2:794776/1‑141 (MQ=255)
                                                                                                                       gACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGcac                   >  3:347753/1‑141 (MQ=255)
                                                                                                                        aCAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGtgtgtgct                  <  4:50500/141‑8 (MQ=255)
                                                                                                                           gCGACTCAGACGCGGAGAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCacacac               <  2:447309/141‑1 (MQ=17)
                                                                                                                              aCTCAGACTCTGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATACAGATCGCGACTCAGACTCAGACAGCGAGACAGACCCAGACATCGACTCAGACTCAGAGGCAGAAAAGCACACACCTg            >  2:371593/1‑141 (MQ=17)
                                                                                                                               cTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt           <  1:665396/141‑1 (MQ=255)
                                                                                                                               cTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCTGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGt           <  2:519350/141‑1 (MQ=255)
                                                                                                                                tCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGtt          >  1:321227/1‑141 (MQ=255)
                                                                                                                                 cAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTa         <  1:745110/141‑1 (MQ=255)
                                                                                                                                 cAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcagac                                     >  4:218771/1‑113 (MQ=255)
                                                                                                                                 cAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcagac                                     <  3:218771/113‑1 (MQ=255)
                                                                                                                                   gACTCGGATAGTGATTCAGACTCAGATAGCGACTCCGACTCGGATAGCGATTCAGATAAAGATAGCGTCTCAGACTCAGTCATCTATTCTGCCTCAGTCAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTaaa       <  1:44433/141‑1 (MQ=21)
                                                                                                                                     cTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAAcc     >  4:323312/1‑141 (MQ=255)
                                                                                                                                        ggATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAAt  >  1:528231/1‑141 (MQ=255)
                                                                                                                                        ggATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAAt  >  1:95478/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                            
ACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATGCAGGTAAGCACACACCTGTTAAACCAAT  >  USA300TCH1516_ALE/618614‑618890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: