Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 632,109 C→G 5.0% V78V (GTC→GTG proP → Proline/betaine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE632,1090CG 5.0% 473.2 / 14.5 140V78V (GTC→GTGproPProline/betaine transporter
Reads supporting (aligned to +/- strand):  ref base C (62/71);  new base G (3/4);  total (65/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 143 total aligned reads displayed.

TTCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTATTATT  >  USA300TCH1516_ALE/631969‑632249
                                                                                                                                            |                                                                                                                                             
ttCGATTTTGATGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAATTGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTc                                                                                                                                               <  2:102933/141‑1 (MQ=255)
 tCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCATACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCg                                                                                                                                              <  2:58124/141‑1 (MQ=255)
 tCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCg                                                                                                                                              >  1:618191/1‑141 (MQ=255)
  cGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGt                                                                                                                                             >  2:935185/1‑141 (MQ=255)
     ttttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTAtt                                                                                                                                          <  1:861613/141‑1 (MQ=255)
     ttttGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTAtt                                                                                                                                          <  1:387580/141‑1 (MQ=255)
       ttGGTGGCTATGCATATACAACTGCGTACGTTGGCGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGGAGCATTAGCCATTGCGTTTTTATTAAGCCCAATTGGTGGTGTCGTATTTg                                                                                                                                        <  2:640879/141‑1 (MQ=255)
         ggTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGt                                                                                                                                      >  4:364410/1‑141 (MQ=255)
         ggTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGt                                                                                                                                      <  1:894669/141‑1 (MQ=255)
         ggTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGt                                                                                                                                      <  1:534136/141‑1 (MQ=255)
           tgtATATGCATATACAACTGAGTACAGTGGAGCGAACTTCTTCTCTCCAGTAGGGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGtat                                                                                                                                    <  4:181127/141‑1 (MQ=255)
            gtCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCACTGAGTTTCTACTACGACCAATTGGTGGTGTCGTATTTGGtatt                                                                                                                                   <  2:48484/141‑1 (MQ=255)
                 tGCATATACATCTGCGTACAGTGGAGCGAACTTCTCCGCTCCAGTAGAGAATGCAGACACTCGACATATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTgg                                                                                                                              <  2:239263/141‑1 (MQ=255)
                  gCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTGGTATTTGGTATTATTGGt                                                                                                                             <  2:683789/141‑1 (MQ=255)
                     tataCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGAc                                                                                                                          <  4:31628/141‑1 (MQ=255)
                        aCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGGATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACaaa                                                                                                                       >  1:689534/1‑141 (MQ=255)
                        aCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACaaa                                                                                                                       >  4:268439/1‑141 (MQ=255)
                        aCAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACaaa                                                                                                                       >  2:740722/1‑141 (MQ=255)
                           aCTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAatat                                                                                                                    >  1:381324/1‑141 (MQ=255)
                             tGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATgg                                                                                                                  >  2:822114/1‑141 (MQ=255)
                              gCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGa                                                                                                                 <  2:136430/141‑1 (MQ=255)
                              gCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGa                                                                                                                 <  2:963280/141‑1 (MQ=255)
                              gCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGa                                                                                                                 <  1:649084/141‑1 (MQ=255)
                               cGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGAc                                                                                                                >  3:13548/1‑141 (MQ=255)
                                gTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACg                                                                                                               <  2:295732/141‑1 (MQ=255)
                                gTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACg                                                                                                               >  2:998143/1‑141 (MQ=255)
                                   cATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTaa                                                                                                            >  2:226569/1‑141 (MQ=255)
                                      tGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGt                                                                                                         <  3:170651/141‑1 (MQ=255)
                                         aGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGt                                                                                                      <  2:307092/141‑1 (MQ=255)
                                         aGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGt                                                                                                      <  2:250871/141‑1 (MQ=255)
                                          gCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTa                                                                                                     >  2:920787/1‑141 (MQ=255)
                                                 tcttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACAt                                                                                              >  3:357412/1‑141 (MQ=255)
                                                  cttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATc                                                                                             >  2:405787/1‑141 (MQ=255)
                                                  cttctCTCCAGTAGAGAATGCAGACAGTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTGGTATTAAGACCAATTGGTGGTGCCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATGAACATc                                                                                             >  4:206418/1‑141 (MQ=255)
                                                   ttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCt                                                                                            <  2:381324/141‑1 (MQ=255)
                                                   ttctCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAAACCAATTGGTGGTGTCGTATATGGTATTATTGGTGACAAATATGGACGTAAAGTTGTTTTAACATCt                                                                                            >  1:368359/1‑141 (MQ=255)
                                                    tctctcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTa                                                                                           >  1:543041/1‑141 (MQ=255)
                                                      tctcCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACa                                                                                         >  3:369012/1‑141 (MQ=255)
                                                         ccAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTCTTAACATCTACAatt                                                                                      >  2:71803/1‑141 (MQ=255)
                                                         ccAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAatt                                                                                      <  1:439290/141‑1 (MQ=255)
                                                             tAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTAttt                                                                                 >  2:457420/1‑141 (MQ=255)
                                                              agagAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTAtttt                                                                                >  1:197776/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             >  2:754139/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             <  2:1560/141‑1 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             >  2:719169/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             >  4:269169/1‑141 (MQ=255)
                                                                 gaATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAAt                                                                             <  1:117012/141‑1 (MQ=255)
                                                                   aTGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATgg                                                                           <  1:101523/141‑1 (MQ=255)
                                                                   aTGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATgg                                                                           >  4:309521/1‑141 (MQ=255)
                                                                    tGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGc                                                                          <  1:196390/141‑1 (MQ=255)
                                                                          cATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTc                                                                    <  3:85963/141‑1 (MQ=255)
                                                                              cGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACa                                                                >  2:984812/1‑141 (MQ=255)
                                                                              cGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACa                                                                <  1:1019155/141‑1 (MQ=255)
                                                                               gACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACAt                                                               >  1:403425/1‑141 (MQ=255)
                                                                                aCAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACAtt                                                              <  1:318173/141‑1 (MQ=255)
                                                                                 cAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTa                                                             <  4:444487/141‑1 (MQ=255)
                                                                                 cAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTa                                                             <  2:618191/141‑1 (MQ=255)
                                                                                  aaaTGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTaa                                                            >  1:188765/1‑141 (MQ=255)
                                                                                    aTGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAAcc                                                          <  1:993982/141‑1 (MQ=255)
                                                                                     tGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCa                                                         <  3:98214/141‑1 (MQ=255)
                                                                                      tctGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCAt                                                        <  1:320992/139‑1 (MQ=255)
                                                                                      gTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCAt                                                        <  2:790420/141‑1 (MQ=255)
                                                                                       ttGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCAtt                                                       <  1:740722/141‑1 (MQ=255)
                                                                                        tGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTg                                                      >  1:1022231/1‑141 (MQ=255)
                                                                                         gACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTgg                                                     >  1:156040/1‑141 (MQ=255)
                                                                                         gACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTgg                                                     >  1:502457/1‑141 (MQ=255)
                                                                                         gACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTgg                                                     >  1:55699/1‑141 (MQ=255)
                                                                                          aCTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGa                                                    <  1:106934/141‑1 (MQ=255)
                                                                                             ttCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGatta                                                 <  2:382203/141‑1 (MQ=255)
                                                                                                gcagcaTTAGCCATTGCGTTTTTATTAAGACCAATTGGCGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTg                                              >  1:812477/1‑141 (MQ=255)
                                                                                                    caTTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCaa                                          >  1:576885/1‑141 (MQ=255)
                                                                                                    caTTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCaa                                          <  1:803741/141‑1 (MQ=255)
                                                                                                      ttAGCCATTGCGTTTTTNTTAAGACCAATTGGTNGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGc                                        >  1:705533/1‑141 (MQ=255)
                                                                                                      ttAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAg                                         >  2:822470/1‑141 (MQ=255)
                                                                                                         gCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTAt                                     <  2:797083/141‑1 (MQ=255)
                                                                                                         gCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTAt                                     >  3:436522/1‑141 (MQ=255)
                                                                                                           cATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGa                                   <  4:205833/141‑1 (MQ=255)
                                                                                                                   ttttATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTg                           <  3:269169/141‑1 (MQ=255)
                                                                                                                     ttattaAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGa                         <  1:935185/141‑1 (MQ=255)
                                                                                                                      tattaAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGAc                        <  2:528700/141‑1 (MQ=255)
                                                                                                                          aaGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTg                    >  1:167023/1‑141 (MQ=255)
                                                                                                                           aGACCAATTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTgg                   <  1:716092/141‑1 (MQ=255)
                                                                                                                           aGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTgg                   <  3:218189/141‑1 (MQ=255)
                                                                                                                              ccAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCa                <  2:156040/141‑1 (MQ=255)
                                                                                                                                aaTTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCAcc              >  2:939787/1‑141 (MQ=255)
                                                                                                                                aaTTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCAcc              <  1:275485/141‑1 (MQ=255)
                                                                                                                                 aTTGGTGGTGTGGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCa             >  2:213106/1‑141 (MQ=255)
                                                                                                                                 aTTGGTGGTGTCGTATTTGGTGTTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTGTTTTAATGGCATTTTCAACATTAACCATTGTATTATTGCCAAGCGATGATCAAATTGGACTTTGGGAACCa             >  4:131461/1‑141 (MQ=255)
                                                                                                                                   tggtggTGTCGTATTTGGTATTATTGGTGACAAATATGGGCGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAAt           <  2:457616/141‑1 (MQ=255)
                                                                                                                                    ggtggtGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTACTGGCATTTTCAAAATTACACCTTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATa          >  3:41013/1‑141 (MQ=255)
                                                                                                                                    ggtggtGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATa          >  3:378968/1‑141 (MQ=255)
                                                                                                                                      tggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTACCCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACt        <  1:919719/141‑1 (MQ=255)
                                                                                                                                      tggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACt        <  2:849091/141‑1 (MQ=255)
                                                                                                                                       ggtgTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACta       >  1:428519/1‑141 (MQ=255)
                                                                                                                                        gtgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtat      >  2:198742/1‑141 (MQ=255)
                                                                                                                                         tgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtatt     >  1:879824/1‑141 (MQ=255)
                                                                                                                                         tgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtatt     <  1:599787/141‑1 (MQ=255)
                                                                                                                                         tgtCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtatt     >  2:412272/1‑141 (MQ=255)
                                                                                                                                           tCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACtattat   <  1:224336/141‑1 (MQ=255)
                                                                                                                                            cGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTattatt  <  1:455736/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TTCGATTTTGGTGTCTATGCATATACAACTGCGTACATTGGAGCGAACTTCTTCTCTCCAGTAGAGAATGCAGACATTCGACAAATGTTGACTTTCGCAGCATTAGCCATTGCGTTTTTATTAAGACCAATTGGTGGTGTCGTATTTGGTATTATTGGTGACAAATATGGACGTAAAGTTGTATTAACATCTACAATTATTTTAATGGCATTTTCAACATTAACCATTGGATTATTGCCAAGCTATGATCAAATTGGACTTTGGGCACCAATACTATTATT  >  USA300TCH1516_ALE/631969‑632249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: