BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	657,770	G→T	6.3%	V62V (GTG→GTT)	USA300HOU_RS03150 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	657,770	0	G	T	6.3%	376.9 / 8.0	117	V62V (GTG→GTT)	USA300HOU_RS03150	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (52/57); new base T (5/3); total (57/60)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

AAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTA  >  USA300TCH1516_ALE/657636‑657909

aaaTATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCt                                                                                                                                       >  4:242199/1‑141 (MQ=255)

  atatAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGc                                                                                                                                     >  2:711568/1‑141 (MQ=255)

  atatAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATAATTTCCTTTTATTAATTGGTTCATCCCCAACAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGc                                                                                                                                     >  3:214906/1‑141 (MQ=255)

   tataATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCt                                                                                                                                    <  3:164784/141‑1 (MQ=255)

   tataATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAAAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCt                                                                                                                                    <  2:247325/141‑1 (MQ=255)

    ataATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAACTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTa                                                                                                                                   >  3:120365/1‑141 (MQ=255)

       atgatgAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTaaaa                                                                                                                                >  2:389463/1‑141 (MQ=255)

          atgaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGa                                                                                                                             <  1:630180/141‑1 (MQ=255)

          atgaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGa                                                                                                                             <  1:1040079/141‑1 (MQ=255)

          atgaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGa                                                                                                                             >  4:187135/1‑141 (MQ=255)

           tgaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGaa                                                                                                                            <  2:1054330/141‑1 (MQ=255)

            gaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGGGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAAt                                                                                                                           <  2:933150/141‑1 (MQ=255)

            gaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAAt                                                                                                                           <  1:245424/141‑1 (MQ=255)

            gaGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAAt                                                                                                                           >  3:148010/1‑141 (MQ=255)

             aGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTTTGAAACCTGCTAAAAAGAATg                                                                                                                          >  2:920545/1‑141 (MQ=255)

               tACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATGCGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAAtatt                                                                                                                        <  2:245846/141‑4 (MQ=255)

               tACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCCaaaaaacattct                                                                                                                        <  1:930075/141‑8 (MQ=255)

               tACTAAATGAACGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGtt                                                                                                                        <  3:184435/141‑1 (MQ=255)

                   tAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGtttttt                                                                                                                    <  1:601681/141‑1 (MQ=255)

                    aaTGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTg                                                                                                                   <  3:400761/141‑1 (MQ=255)

                     aTGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTgg                                                                                                                  <  4:319253/141‑1 (MQ=255)

                     aTGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTgg                                                                                                                  <  2:681027/141‑1 (MQ=255)

                       gATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGcc                                                                                                                >  4:265994/1‑141 (MQ=255)

                       gATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGcc                                                                                                                <  1:484502/141‑1 (MQ=255)

                           gATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGtt                                                                                                            >  2:118642/1‑141 (MQ=255)

                             tataATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCTATTTgt                                                                                                          >  2:943521/1‑140 (MQ=255)

                               taataaGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAg                                                                                                        <  1:985606/141‑1 (MQ=255)

                                     gCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTAtct                                                                                                  <  3:287074/141‑1 (MQ=255)

                                      cACTTGGATTAGTTTATTTTTTTTTTTTTTTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGTTAtctt                                                                                                 >  4:32727/1‑140 (MQ=255)

                                       aCTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATctct                                                                                                <  3:356393/141‑1 (MQ=255)

                                       aCTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTACTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATctct                                                                                                <  4:327482/141‑1 (MQ=255)

                                         ttGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTTCAGGTATCTCTAc                                                                                              >  4:107580/1‑141 (MQ=255)

                                          tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                             <  4:332296/141‑1 (MQ=255)

                                          tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                             >  3:281570/1‑141 (MQ=255)

                                          tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                             <  1:807792/141‑1 (MQ=255)

                                          tGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCAAAACGATAAGCCAAAATTAGTAGAGAAGCGTTTGCAAAATTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACt                                                                                             <  1:648309/141‑1 (MQ=11)

                                               tAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTAtt                                                                                        >  1:292290/1‑141 (MQ=255)

                                                aGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTc                                                                                       >  4:330267/1‑141 (MQ=255)

                                                aGTTTATTTATTCCTCTTATTAATTGGATCAACCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAAACTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTc                                                                                       <  2:542795/141‑1 (MQ=17)

                                                  tttATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGc                                                                                     <  3:77421/141‑1 (MQ=255)

                                                  tttATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGc                                                                                     >  1:557917/1‑141 (MQ=255)

                                                  tttATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGc                                                                                     >  1:557890/1‑141 (MQ=255)

                                                      ttttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGtt                                                                                 >  1:690786/1‑141 (MQ=255)

                                                       tttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTa                                                                                >  1:1077029/1‑141 (MQ=255)

                                                        ttttCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTAc                                                                               <  2:38626/141‑1 (MQ=255)

                                                           tttttttttTAATTGGTTAATCCCCAAAAGATACGTCAAAATTAGTAGTGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAATTATAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGTAGTTACAtt                                                                            >  2:1072261/4‑141 (MQ=18)

                                                             cTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTaa                                                                          <  1:428869/141‑1 (MQ=255)

                                                             cTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTaa                                                                          <  2:984283/141‑1 (MQ=255)

                                                               tttATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGa                                                                        <  1:305507/141‑1 (MQ=255)

                                                                tttttaATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTTGAGAAGAGTTTGAATATTTAAATATTTTGATTTTTTTTTTTTTTAATGTTTTTTGTCCATTTTCAGTTATCTCTACTTTATTCGCAGTTACATTAAGaa                                                                       >  4:365731/4‑141 (MQ=255)

                                                                 tattaATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTGTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGaaa                                                                      <  3:420528/141‑1 (MQ=255)

                                                                    taATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGta                                                                   >  3:173059/1‑141 (MQ=255)

                                                                      aTTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGtata                                                                 <  4:352817/141‑1 (MQ=255)

                                                                       ttGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGTCTTGTTGCAGGTATCTCTACTTTATTTGCAGTTACATTAAGAAAGTATac                                                                >  2:826652/1‑141 (MQ=255)

                                                                       ttGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATac                                                                >  1:1051422/1‑141 (MQ=255)

                                                                        tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAg                                                                                   <  1:93137/121‑1 (MQ=255)

                                                                        tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATaca                                                               <  2:523797/141‑1 (MQ=255)

                                                                        tGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATaca                                                               >  3:257338/1‑141 (MQ=255)

                                                                         ggTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATacac                                                              >  1:796443/1‑141 (MQ=255)

                                                                          gTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATAcaca                                                             <  3:308607/141‑1 (MQ=255)

                                                                          gTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATAcaca                                                             <  1:993422/141‑1 (MQ=255)

                                                                           ttCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATacgctt                                                            >  1:823826/1‑137 (MQ=255)

                                                                            tCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTTTTTTTTTTCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACAtt                                                           >  2:303024/1‑141 (MQ=21)

                                                                              aTCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTa                                                         >  1:423001/1‑141 (MQ=255)

                                                                               tCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTTAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCTAGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACGCTTTTAc                                                        <  2:823826/141‑1 (MQ=18)

                                                                               tCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTAc                                                        >  2:158859/1‑141 (MQ=255)

                                                                                  ccAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTTTACACATTTACTTg                                                     <  2:222546/141‑1 (MQ=255)

                                                                                  ccAAAAGATACGTCAAAACTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTg                                                     <  4:120365/141‑1 (MQ=255)

                                                                                   cAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGa                                                    >  2:1063434/1‑141 (MQ=255)

                                                                                   cAAAAGATACGTAAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGa                                                    <  2:780642/141‑1 (MQ=255)

                                                                                      aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                 <  1:842400/141‑1 (MQ=255)

                                                                                      aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                 <  2:312410/141‑1 (MQ=255)

                                                                                      aaGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGacac                                                 <  2:312421/141‑1 (MQ=255)

                                                                                       aGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACt                                                <  2:456143/141‑1 (MQ=255)

                                                                                       aGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACt                                                <  1:118107/141‑1 (MQ=255)

                                                                                       aGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACt                                                <  2:615034/141‑1 (MQ=255)

                                                                                        gATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTc                                               <  1:389463/141‑1 (MQ=255)

                                                                                         aTACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCa                                              >  1:1086574/1‑141 (MQ=255)

                                                                                           aCGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAAc                                            >  4:175196/1‑141 (MQ=255)

                                                                                            cGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACt                                           <  1:1067487/141‑1 (MQ=255)

                                                                                               cAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGa                                        <  2:991457/141‑1 (MQ=255)

                                                                                                aaaaTTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGAt                                       <  4:55401/141‑1 (MQ=255)

                                                                                                aaaaTTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGAt                                       <  2:839549/141‑1 (MQ=255)

                                                                                                  aaTTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAg                                     >  3:168105/1‑141 (MQ=255)

                                                                                                      agtagAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGaaaa                                 >  3:376341/1‑141 (MQ=255)

                                                                                                       gtagAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTTTTTTTTTTTTTGTATCTCTACTTTATTCACATTTATATTAAGAAATTATACACATTTACTTGACATTCAACTTGATAGaaatt                                >  2:980313/1‑139 (MQ=17)

                                                                                                         agagAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTa                              >  1:830542/1‑141 (MQ=255)

                                                                                                          gagaAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAg                             <  1:314675/141‑1 (MQ=255)

                                                                                                           agaagaGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGTCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGt                            >  4:233967/1‑141 (MQ=255)

                                                                                                               gagTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTAtt                        <  3:327506/141‑1 (MQ=255)

                                                                                                                 gTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGc                      <  1:394627/141‑1 (MQ=255)

                                                                                                                  tttGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCt                     <  4:376990/141‑1 (MQ=255)

                                                                                                                   ttGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCta                    >  4:271772/1‑141 (MQ=255)

                                                                                                                           ttAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATAtgttgt            >  2:658147/1‑141 (MQ=255)

                                                                                                                           ttAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATAtgttgt            >  4:348829/1‑141 (MQ=255)

                                                                                                                            tAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTa           <  2:557917/141‑1 (MQ=255)

                                                                                                                              aaTATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATc         >  4:258595/1‑141 (MQ=255)

                                                                                                                                tatTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATcac       >  1:280814/1‑141 (MQ=255)

                                                                                                                                   tgtgAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACAtt    <  3:7014/141‑1 (MQ=255)

                                                                                                                                     tgAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTta  <  1:977810/141‑1 (MQ=255)

AAATATAATGATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATTAATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAATATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTCGCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTTATTGCTATATGTTGTATCACATTTA  >  USA300TCH1516_ALE/657636‑657909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`