BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	732,682	T→C	5.1%	Y235Y (TAT→TAC)	USA300HOU_RS03560 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	732,682	0	T	C	5.1%	353.9 / 7.2	117	Y235Y (TAT→TAC)	USA300HOU_RS03560	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (57/54); new base C (2/4); total (59/58)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 124 total aligned reads displayed.

GTGAAAACAGAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATTC  >  USA300TCH1516_ALE/732542‑732821

gtgAAAACAGAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAatat                                                                                                                                             >  1:34348/1‑141 (MQ=255)

 tgAAAACAGAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAAtata                                                                                                                                            >  2:566356/1‑141 (MQ=255)

    aaaCAGAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAg                                                                                                                                         <  1:249013/141‑1 (MQ=255)

      aCAGAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGtt                                                                                                                                       <  1:615120/141‑1 (MQ=255)

         gAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAAt                                                                                                                                    <  2:978210/141‑1 (MQ=255)

         gAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAAt                                                                                                                                    <  1:695737/141‑1 (MQ=255)

         aaaaaaTCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAAt                                                                                                                                    <  1:350656/140‑1 (MQ=255)

           aaaaTCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCa                                                                                                                                  >  2:587488/1‑141 (MQ=255)

            aaaTCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATCTACAGTTAATCaa                                                                                                                                 >  1:945106/1‑141 (MQ=255)

               tCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAggg                                                                                                                              <  3:211420/141‑1 (MQ=255)

               tCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAggg                                                                                                                              >  2:82365/1‑141 (MQ=255)

               tCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAggg                                                                                                                              <  2:780337/141‑1 (MQ=255)

               tCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGACGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATACACAGTTAATCAAggg                                                                                                                              <  1:163146/141‑1 (MQ=255)

               tCACATGATCAATGTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAggg                                                                                                                              <  4:238862/141‑1 (MQ=255)

                cacaTGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGa                                                                                                                             >  2:239602/1‑141 (MQ=255)

                 acaTGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGaa                                                                                                                            >  1:691301/1‑141 (MQ=255)

                   aTGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGt                                                                                                                          >  1:128350/1‑141 (MQ=255)

                   aTGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGt                                                                                                                          <  1:496286/141‑1 (MQ=255)

                     gATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTa                                                                                                                        <  1:569119/141‑1 (MQ=255)

                        cAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGaa                                                                                                                     <  4:78660/141‑1 (MQ=255)

                          aTTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAAtt                                                                                                                   >  3:94149/1‑141 (MQ=255)

                           tttAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAAttt                                                                                                                  >  2:114088/1‑141 (MQ=255)

                           tttAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAAttt                                                                                                                  >  2:114121/1‑141 (MQ=255)

                              aaGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGa                                                                                                               <  1:799417/141‑1 (MQ=255)

                              aaGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGa                                                                                                               >  1:456162/1‑141 (MQ=255)

                                gATATATAATAAGAATAAGGTGCGACCGCTCGCAGAATGTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAAATTTAATGAGTGGAAGATCAGTGAAAAAACATACAGTTAATCTAGGGTAGTTAGAATTTAGAtg                                                                                                             <  4:452433/141‑2 (MQ=255)

                                          aaGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAagag                                                                                                   <  2:489832/141‑1 (MQ=255)

                                             aaTAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCg                                                                                                <  1:869114/141‑1 (MQ=255)

                                             aaTAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCg                                                                                                <  1:566356/141‑1 (MQ=255)

                                              aTAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCgg                                                                                               <  2:65554/141‑1 (MQ=255)

                                              aTAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCgg                                                                                               <  2:722855/141‑1 (MQ=255)

                                               tagggTGCGACCGCTCGAAGTACTGAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGCAGTTTAGTGAAAAAATATACAGTTAATCAAGCGAAGTTAGAATGTAGATTAATCAAAGAGCCgtc                                                                                              >  4:411127/4‑139 (MQ=255)

                                                aaGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCa                                                                                             <  1:529608/141‑1 (MQ=255)

                                                 aGGTGCGACCGCTCGCAGTATTTAACATAGAAGAAACAGCTGATGGAATAGTAACTTTAATGTTATGGAGTGGAAGTTTAGTGAAAAAGTATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAg                                                                                            >  2:368718/1‑141 (MQ=255)

                                                 aGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATACCTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCATGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAg                                                                                            <  1:782890/141‑1 (MQ=255)

                                                 aGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAg                                                                                            >  3:133553/1‑141 (MQ=255)

                                                 aGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAg                                                                                            >  1:432662/1‑141 (MQ=255)

                                                 aGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAg                                                                                            >  3:133651/1‑141 (MQ=255)

                                                 aGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAg                                                                                            >  2:12329/1‑141 (MQ=255)

                                                  ggcgcgcCCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGt                                                                                           <  1:501505/138‑1 (MQ=255)

                                                  ggTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGt                                                                                           >  2:576322/1‑141 (MQ=255)

                                                   gTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTc                                                                                          >  1:1072848/1‑141 (MQ=255)

                                                     gCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGt                                                                                        >  1:1017216/1‑141 (MQ=255)

                                                      cGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGtt                                                                                       <  2:988911/141‑1 (MQ=255)

                                                      cGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGtt                                                                                       >  1:662131/1‑141 (MQ=255)

                                                          cGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTGATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTtata                                                                                   <  3:332257/141‑1 (MQ=255)

                                                                aaGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATc                                                                             <  2:682671/140‑1 (MQ=255)

                                                                    aTTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCtata                                                                         <  1:959887/141‑1 (MQ=255)

                                                                         aaaTAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGAtt                                                                    <  1:334255/141‑1 (MQ=255)

                                                                                 gaaACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCt                                                            >  3:243576/1‑141 (MQ=255)

                                                                                  aaaCAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTc                                                           >  2:942138/1‑141 (MQ=255)

                                                                                  aaaCAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTc                                                           >  2:53003/1‑141 (MQ=255)

                                                                                   aaCAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCg                                                          >  2:169555/1‑141 (MQ=255)

                                                                                   aaCAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCg                                                          >  2:170049/1‑141 (MQ=255)

                                                                                    aCAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGa                                                         <  3:312419/141‑1 (MQ=255)

                                                                                    aCAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGa                                                         <  3:301833/141‑1 (MQ=255)

                                                                                     cAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGat                                                        >  2:331552/1‑141 (MQ=255)

                                                                                     cAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGat                                                        >  1:663162/1‑141 (MQ=255)

                                                                                     cAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGat                                                        >  3:119295/1‑141 (MQ=255)

                                                                                       gCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGAtt                                                                                                             >  2:125229/1‑86 (MQ=255)

                                                                                        cTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGattat                                                     <  2:744372/141‑1 (MQ=255)

                                                                                         tGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGattatt                                                    <  2:691301/141‑1 (MQ=255)

                                                                                           aTGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTAtttt                                                  >  2:204617/1‑141 (MQ=255)

                                                                                            tGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTg                                                 <  2:548881/141‑1 (MQ=255)

                                                                                              gAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGc                                               >  1:814187/1‑141 (MQ=255)

                                                                                                  aataaCTTTAATGTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGAt                                           <  3:82115/141‑1 (MQ=255)

                                                                                                    taaCTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGAttt                                         <  1:978427/141‑1 (MQ=255)

                                                                                                     aaCTTTCATTTTACTGAATAGAAGTTTAGTGAAAAAATATACAGATAATCACGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTCTACGATTATATCCCTCGATTATTTTGGCCGATtta                                        <  2:457604/141‑1 (MQ=255)

                                                                                                       cTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATttatt                                      >  1:399740/1‑141 (MQ=255)

                                                                                                         ttAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTAc                                    >  4:334968/1‑141 (MQ=255)

                                                                                                         ttAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTAc                                    <  2:76755/141‑1 (MQ=255)

                                                                                                         ttAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTAc                                    <  3:71714/141‑1 (MQ=255)

                                                                                                            aTTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGACGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTAGTTTGGCCGGTTTATTACttt                                 >  3:340607/1‑141 (MQ=255)

                                                                                                             ttttATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTta                                >  4:386975/1‑141 (MQ=255)

                                                                                                             ttttATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTta                                <  2:804616/141‑1 (MQ=255)

                                                                                                             ttttATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCAGATTTATTACTTta                                >  2:372908/1‑141 (MQ=255)

                                                                                                                 aTTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACa                            >  1:51762/1‑141 (MQ=255)

                                                                                                                 aTTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACa                            <  2:421590/141‑1 (MQ=255)

                                                                                                                 aTTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACa                            <  1:212588/141‑1 (MQ=255)

                                                                                                                  ttGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACaa                           <  2:189426/141‑1 (MQ=255)

                                                                                                                  ttGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATAAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACaa                           >  2:361452/1‑141 (MQ=255)

                                                                                                                      gTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCAc                       <  1:916350/141‑1 (MQ=255)

                                                                                                                      gTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCAc                       >  4:314820/1‑141 (MQ=255)

                                                                                                                      gTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCCTCTACATCAATATCTAAAATCATAAATATCGATTCTTTTGGCCGATTTATAACTTTATACAAGCAc                       >  1:644155/1‑141 (MQ=255)

                                                                                                                         gAAGTTTAGTGAAAAAATACACAGTTAATCAAGGGGAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCaa                    <  1:659217/141‑1 (MQ=255)

                                                                                                                          aaGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAAAAAAAGAGACGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGAGTATTTTGGCCGATTTCTTACTTTATATAAgaaaaaaa                   <  4:207137/141‑8 (MQ=255)

                                                                                                                            gTTTAGTGAAAAAATACACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGcc                                              >  1:1075477/1‑112 (MQ=255)

                                                                                                                            gTTTAGTGAAAAAATACACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGcc                                              <  2:1075477/112‑1 (MQ=255)

                                                                                                                             tttAGTGAAAAAATACACAGTTAATCAAGGGGAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCa                <  2:37538/141‑1 (MQ=255)

                                                                                                                              ttAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCaa               >  1:441533/1‑141 (MQ=255)

                                                                                                                               tAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCaaa              <  1:1010997/141‑1 (MQ=255)

                                                                                                                                cgTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCaaaa             >  4:438640/2‑141 (MQ=255)

                                                                                                                                aGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCaaaa             >  3:32106/1‑141 (MQ=255)

                                                                                                                                  tGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCaaaaaa           >  2:24579/1‑141 (MQ=255)

                                                                                                                                  tGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCaaaaaa           <  3:343689/141‑1 (MQ=255)

                                                                                                                                  aaaaaaaaTATACAGTTAATAAAGAAAAGTTAAAATATAAATAAATAAAAGAGTCGGCAGTCGTATATATACATCTATACGATTATATACCTCGAGTATTTTGGCCGATTTATTACTTTATACAAGAAAAAATaaaaaaaa           <  2:693230/139‑1 (MQ=255)

                                                                                                                                         atatACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATgatgat    <  1:105923/141‑1 (MQ=255)

                                                                                                                                           ataCAGTTAATCAAGGGAAGTTAGAATTTATATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATTc  >  1:744144/1‑141 (MQ=255)

                                                                                                                                           ataCAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCACTGCAAAAAATGATGATTc  >  1:332425/1‑141 (MQ=255)

                                                                                                                                           ataCAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATTc  >  3:289211/1‑141 (MQ=255)

GTGAAAACAGAAAAATCACATGATCAATTTAAGATATATATTAAGAATAAGGTGCGACCGCTCGCAGTATTTAAAATAGAAGAAACAGCTGATGGAATAATAACTTTAATTTTATTGAGTGGAAGTTTAGTGAAAAAATATACAGTTAATCAAGGGAAGTTAGAATTTAGATTAATCAAAGAGTCGGCAGTCGTTTATATACATCTATACGATTATATCCCTCGATTATTTTGGCCGATTTATTACTTTATACAAGCACCAATGCAAAAAATGATGATTC  >  USA300TCH1516_ALE/732542‑732821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`