Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 748,209 T→C 5.2% A68A (GCT→GCC oxyR → Hydrogen peroxide‑inducible genes activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE748,2090TC 5.2% 425.4 / 8.6 146A68A (GCT→GCCoxyRHydrogen peroxide‑inducible genes activator
Reads supporting (aligned to +/- strand):  ref base T (62/76);  new base C (4/4);  total (66/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

CTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGC  >  USA300TCH1516_ALE/748070‑748349
                                                                                                                                           |                                                                                                                                            
cTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTc                                                                                                                                             <  2:27574/141‑1 (MQ=255)
    caTTTTATATGTATCACAACATTCTGTAACACAAAGACTTAAAGCCATTGAAAATGCTTCTCGAGTAGATATGTATAAAAAAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGa                                                                                                                                         <  2:1034159/140‑1 (MQ=255)
     aTTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGAc                                                                                                                                        >  4:353366/1‑141 (MQ=255)
        ttATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTAGTGACATg                                                                                                                                     <  1:230342/141‑1 (MQ=255)
        ttATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTAAGCATGCTCGTGACATg                                                                                                                                     >  2:870158/1‑141 (MQ=255)
            atatatCTCCACCTGATTTTACAAAAAGAATAAAAGCTATTTAAAATGCTTTTGGAGTAGATATTTTTATCAAAAAAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGa                                                                                                                                 <  2:798500/141‑1 (MQ=255)
            atatatCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGa                                                                                                                                 >  2:498971/1‑141 (MQ=255)
            atatatCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTAATAAAAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGa                                                                                                                                 <  2:367225/141‑1 (MQ=255)
                   tcAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAagaga                                                                                                                          <  3:200009/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTTTTATAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      >  2:477030/1‑141 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACATCTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      <  1:1003235/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      <  1:223823/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      <  3:95808/141‑1 (MQ=255)
                       ccTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGa                                                                                                                      >  2:536022/1‑141 (MQ=255)
                        ctgctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGat                                                                                                                     <  4:310627/141‑1 (MQ=255)
                         tgctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGatt                                                                                                                    >  2:327086/1‑141 (MQ=255)
                          gctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGatta                                                                                                                   >  1:354037/1‑141 (MQ=255)
                          gctgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGatta                                                                                                                   >  2:829052/1‑141 (MQ=255)
                            tgTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATTATTATTTAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTTAAAAGAGAGCGattatt                                                                                                                 >  2:1738/1‑141 (MQ=255)
                             gTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTAttt                                                                                                                >  2:303039/1‑141 (MQ=255)
                             gTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTAttt                                                                                                                >  2:838305/1‑141 (MQ=255)
                             gTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATCAttt                                                                                                                <  1:543251/141‑1 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  2:321021/141‑1 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  2:1029848/141‑1 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  1:430912/141‑1 (MQ=255)
                                  acaAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGa                                                                                                           <  2:437785/141‑1 (MQ=255)
                                   caAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGAc                                                                                                          <  2:610200/141‑1 (MQ=255)
                                   caAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGAc                                                                                                          >  4:303753/1‑141 (MQ=255)
                                       gACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACaaaa                                                                                                      >  1:617344/1‑141 (MQ=255)
                                          tAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGc                                                                                                   >  2:311372/1‑141 (MQ=255)
                                           aaaaGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCa                                                                                                  >  1:504348/1‑141 (MQ=255)
                                               gCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGca                                                                                              >  4:146123/1‑141 (MQ=255)
                                                  aTTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACat                                                                                           <  1:643565/141‑1 (MQ=255)
                                                  aTTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACat                                                                                           <  1:871341/141‑1 (MQ=255)
                                                   ttGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACata                                                                                          >  1:625087/1‑141 (MQ=255)
                                                   ttGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACata                                                                                          >  4:179163/1‑141 (MQ=255)
                                                     gAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGAATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATAtt                                                                                        >  1:597602/1‑141 (MQ=255)
                                                     gAAAATGCATTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTCTTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTCTTTGATAAAATGAAGGCCCATAtt                                                                                        >  2:50792/1‑141 (MQ=255)
                                                      aaaaTGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTTACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTg                                                                                       >  1:46029/1‑141 (MQ=255)
                                                       aaaTGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTgg                                                                                      >  3:291368/1‑141 (MQ=255)
                                                       aaaTGCTTTTGGACTAGATATTTTTAACAAAAAAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTgg                                                                                      <  4:380442/141‑1 (MQ=255)
                                                         aTGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtg                                                                                    <  1:1052772/141‑1 (MQ=255)
                                                          tGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtga                                                                                   >  1:733950/1‑141 (MQ=255)
                                                           gCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaa                                                                                  >  1:454606/1‑141 (MQ=255)
                                                            ctattGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAAGTGAAGGATCAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAGAATGCAGGCACATATTGgtgaag                                                                                 >  2:149483/4‑141 (MQ=255)
                                                            cTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaag                                                                                 <  3:147533/141‑1 (MQ=255)
                                                            cTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGAAGACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgttaag                                                                                 <  1:576222/141‑1 (MQ=255)
                                                            cTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTCATTACAAAAACTGAAGAAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaag                                                                                 <  1:712613/141‑1 (MQ=255)
                                                               ttGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGgtgaagtga                                                                              >  1:87950/1‑141 (MQ=255)
                                                                      aGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       >  3:437933/1‑141 (MQ=255)
                                                                      aGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       >  2:331010/1‑141 (MQ=255)
                                                                      aGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       >  1:654090/1‑141 (MQ=255)
                                                                      aGATATTTTTATAAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAAc                                                                       <  2:430493/141‑1 (MQ=255)
                                                                       gATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACa                                                                      >  1:796778/1‑141 (MQ=255)
                                                                        atatTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACaa                                                                     <  3:110648/141‑1 (MQ=255)
                                                                        atatTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTAAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACaa                                                                     <  3:317338/141‑1 (MQ=255)
                                                                         tatTTTTATAAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAat                                                                    <  2:278481/141‑1 (MQ=255)
                                                                          atTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAata                                                                   >  1:679292/1‑141 (MQ=255)
                                                                           tttttATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAatat                                                                  <  2:183245/141‑1 (MQ=255)
                                                                              ttATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCaa                                                               >  1:885699/1‑141 (MQ=255)
                                                                              ttATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCaa                                                               <  2:986769/141‑1 (MQ=255)
                                                                               tATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAAt                                                              <  1:182773/141‑1 (MQ=255)
                                                                                 tCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCg                                                            <  1:132139/141‑1 (MQ=255)
                                                                                   aGAAAAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCggg                                                          <  2:203923/141‑1 (MQ=255)
                                                                                       cAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGtt                                                      <  1:246288/141‑1 (MQ=255)
                                                                                         aaaaaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGttct                                                    <  2:574219/141‑1 (MQ=255)
                                                                                         aaaaaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGttct                                                    <  1:786965/141‑1 (MQ=255)
                                                                                          aaaaaCAATTGATTACAACAACTGAAGGAACCATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACCTATTGGTGAAGTGAATGGAACAATATCAATCGGGTGttctt                                                   <  4:224212/141‑1 (MQ=255)
                                                                                          aaaaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGttctt                                                   <  4:418868/141‑1 (MQ=255)
                                                                                            aaaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTtcttct                                                 <  1:601972/141‑1 (MQ=255)
                                                                                             aaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTcttctt                                                >  1:321618/1‑141 (MQ=255)
                                                                                             aaCAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTcttctt                                                >  1:937679/1‑141 (MQ=255)
                                                                                                aaTTGCTTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGa                                             <  1:809931/141‑1 (MQ=255)
                                                                                                aaTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGa                                             <  1:617635/141‑1 (MQ=255)
                                                                                                aaTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGa                                             <  1:645706/141‑1 (MQ=255)
                                                                                                 agtGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAAGCGGGTGTGCTTCTTTGAt                                            <  3:148796/139‑1 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  3:186670/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            <  1:1019760/141‑1 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  1:125334/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            <  2:315101/141‑1 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            >  1:993415/1‑141 (MQ=255)
                                                                                                 aTTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGAt                                            <  2:838837/141‑1 (MQ=255)
                                                                                                   tGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTg                                          >  2:21391/1‑141 (MQ=255)
                                                                                                    gATTACAACAACTGAAGGAACAATGATTATTGAGCATGCCCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATGGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTgg                                         <  1:875580/141‑1 (MQ=255)
                                                                                                           acaacTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAAcc                                  >  1:566794/1‑141 (MQ=255)
                                                                                                                  aaGGAACAAGGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTc                           <  2:254756/141‑1 (MQ=255)
                                                                                                                   aGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTcc                          <  1:829052/141‑1 (MQ=255)
                                                                                                                    ggAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCt                         >  2:902793/1‑141 (MQ=255)
                                                                                                                      aaCAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGa                       <  4:276358/141‑1 (MQ=255)
                                                                                                                       aCAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGaa                      <  1:645631/141‑1 (MQ=255)
                                                                                                                       aCAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGaa                      <  2:890495/141‑1 (MQ=255)
                                                                                                                         aaTGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCCATCGGGTGCTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGt                    >  2:747736/1‑141 (MQ=255)
                                                                                                                              ttattGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGa               >  1:655430/1‑141 (MQ=255)
                                                                                                                               tattGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACACACCTTACTTCCTGAAGTTTTAAg              >  2:439699/1‑141 (MQ=255)
                                                                                                                                 ttGAGCATGCTCGTGACATGTTGAAAAGAGGGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGcc            <  2:63339/141‑1 (MQ=255)
                                                                                                                                 ttGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGcc            >  1:627926/1‑141 (MQ=255)
                                                                                                                                  tGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCt           <  2:132217/141‑1 (MQ=255)
                                                                                                                                   gAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCta          <  1:738720/141‑1 (MQ=255)
                                                                                                                                    aGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAAACtat         >  4:353503/1‑141 (MQ=255)
                                                                                                                                           tCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGc  <  1:311372/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTGAAATTTTATATATATCTCAACCTGCTGTTACACAAAGACTAAAAGCTATTGAAAATGCTTTTGGAGTAGATATTTTTATCAGAACAAAAAAACAATTGATTACAACAACTGAAGGAACAATGATTATTGAGCATGCTCGTGACATGTTGAAAAGAGAGCGATTATTTTTTGACAAAATGCAGGCACATATTGGTGAAGTGAATGGAACAATATCAATCGGGTGTTCTTCTTTGATTGGACAAACCTTACTTCCTGAAGTTTTGAGCCTATATAATGC  >  USA300TCH1516_ALE/748070‑748349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: