Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 803,539 A→G 6.2% intergenic (‑239/+31) USA300HOU_RS03920 ← / ← dtpT Putative lipid kinase/Di‑/tripeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE803,5390AG 6.2% 444.0 / 13.5 159intergenic (‑239/+31)USA300HOU_RS03920/dtpTPutative lipid kinase/Di‑/tripeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (60/87);  new base G (6/4);  total (67/92)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

Only 100 of 160 total aligned reads displayed.

AATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCTA  >  USA300TCH1516_ALE/803400‑803679
                                                                                                                                           |                                                                                                                                            
aaTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATATATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGAc                                                                                                                                             <  2:1058144/141‑1 (MQ=255)
aaTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTAGAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGAc                                                                                                                                             <  1:853700/141‑1 (MQ=255)
 aTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACt                                                                                                                                            <  3:40406/141‑1 (MQ=255)
 aTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACt                                                                                                                                            <  1:305066/141‑1 (MQ=255)
  tGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACtt                                                                                                                                           <  1:653735/141‑1 (MQ=255)
  tGAAATTGATTTCTATAGAGTGGAACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACtt                                                                                                                                           <  2:959757/141‑1 (MQ=255)
    aaaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTAc                                                                                                                                         <  3:350110/141‑1 (MQ=255)
    aaaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTAc                                                                                                                                         <  2:625082/141‑1 (MQ=255)
      aTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTg                                                                                                                                       >  2:791325/1‑141 (MQ=255)
       ttGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGt                                                                                                                                      <  1:31092/141‑1 (MQ=255)
       ttGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGt                                                                                                                                      <  1:667848/141‑1 (MQ=255)
        ttacttCTAGCGATTGAGACGAGAATTTGTTTTTATAGCGGACTATTATTAGCGTATTAAGTTTTTATTTATACTGACATCTTGAATTTAATTAATAGAAACCTATATAAAAACAGTATGTCATTTAAATGGCTTACTGtt                                                                                                                                     <  2:630346/137‑1 (MQ=255)
          aTTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttt                                                                                                                                   <  1:45500/141‑1 (MQ=255)
          aTTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttt                                                                                                                                   <  1:45464/141‑1 (MQ=255)
           tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                                  <  1:103496/141‑1 (MQ=255)
           tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                                  <  1:103497/141‑1 (MQ=255)
           tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                                  <  3:310383/141‑1 (MQ=255)
           tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                                  <  2:52844/141‑1 (MQ=255)
             tCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTg                                                                                                                                >  1:445372/1‑141 (MQ=255)
              atataGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACAGTTTTTTGa                                                                                                                               <  1:957589/140‑1 (MQ=255)
                ataGAGTGAGACTAGAAAATATAGTTATAGCATTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAAGTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAAt                                                                                                                             <  3:168512/141‑1 (MQ=255)
                   gagTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAg                                                                                                                          <  2:68728/141‑1 (MQ=255)
                   gagTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAg                                                                                                                          >  2:174945/1‑141 (MQ=255)
                    agTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAgg                                                                                                                         >  3:457171/1‑141 (MQ=255)
                       gggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCca                                                                                                                      <  2:709684/141‑1 (MQ=255)
                        ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                                     >  1:505835/1‑141 (MQ=255)
                        ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                                     >  3:22086/1‑141 (MQ=255)
                        ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                                     >  3:182260/1‑141 (MQ=255)
                         gACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                                    <  3:135579/141‑1 (MQ=255)
                          aCGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCAATTTAAATGACTTACTGTTTTTTGAATTAGGCcaaca                                                                                                                   <  2:189681/141‑1 (MQ=255)
                               aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                              >  4:281830/1‑141 (MQ=255)
                                  atatAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAc                                                                                                           >  1:836730/1‑141 (MQ=255)
                                      aGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtat                                                                                                       <  2:83961/141‑1 (MQ=255)
                                       gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                                      >  4:394819/1‑141 (MQ=255)
                                       gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                                      <  1:951369/141‑1 (MQ=255)
                                         tataGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTAAATTTAATTAATAGAAAACTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGACCACAAAAATTCACCTATAcc                                                                                                    >  4:293399/1‑141 (MQ=255)
                                           taGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtt                                                                                                  <  4:192649/141‑1 (MQ=255)
                                            aGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACATTAAGCCATTTAAATGTCTTACTGTTTTTTGAATTAGGCCAACAACATTAACTAATACCttt                                                                                                 >  2:318608/1‑141 (MQ=255)
                                             gCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTCATAGAAAAATAAACAAAAACCGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtttg                                                                                                <  1:1028971/141‑2 (MQ=255)
                                             gCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTc                                                                                                >  1:16208/1‑141 (MQ=255)
                                              cTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCa                                                                                               <  1:736514/141‑1 (MQ=255)
                                                   tataATGATCATATTAACTTTTTATTTATACTGATATCTTGAATTTAATTTATAGAAACCTATAAAAAAACAGTAACCCATTTAAATGCCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATCCCTTTCATCGCt                                                                                          <  4:192937/141‑1 (MQ=255)
                                                   tataATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATcaaa                                                                                          <  1:558300/141‑4 (MQ=255)
                                                       aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                                      <  1:568656/141‑1 (MQ=255)
                                                       aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                                      <  1:569004/141‑1 (MQ=255)
                                                       aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTAATcatcaaag                                                                                      <  2:236766/141‑8 (MQ=255)
                                                        tGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgat                                                                                     >  2:1031349/1‑141 (MQ=255)
                                                         gAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgatg                                                                                    <  2:917539/141‑1 (MQ=255)
                                                            cATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGAtt                                                                                 >  2:421424/1‑141 (MQ=255)
                                                            cATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGAtt                                                                                 >  1:33812/1‑141 (MQ=255)
                                                               atTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTaaa                                                                              >  2:438854/1‑141 (MQ=255)
                                                               atTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTaaa                                                                              >  3:249648/1‑141 (MQ=255)
                                                                ttAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAg                                                                             <  2:162221/141‑1 (MQ=255)
                                                                 tAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                            >  2:737232/1‑141 (MQ=255)
                                                                   aGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTg                                                                          <  1:323840/141‑1 (MQ=255)
                                                                       tttatttaTACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGAt                                                                      <  3:410360/141‑1 (MQ=255)
                                                                        ttatttatACTGATATCTTGAAATTAATTAATAGAAACCGATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAATAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGTTGAGAtt                                                                     >  2:106950/1‑141 (MQ=255)
                                                                         tatttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTg                                                                    >  3:100038/1‑141 (MQ=255)
                                                                           tttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCt                                                                  <  2:717704/141‑1 (MQ=255)
                                                                           tttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCt                                                                  <  1:886260/141‑1 (MQ=255)
                                                                              ataCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGTCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAAt                                                               <  4:219955/141‑1 (MQ=255)
                                                                               taCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATa                                                              >  3:301043/1‑141 (MQ=255)
                                                                                  tGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACaa                                                           <  1:806172/141‑1 (MQ=255)
                                                                                      atCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAttgt                                                       >  4:152517/1‑141 (MQ=255)
                                                                                          tGAATCTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                                   <  1:110131/141‑1 (MQ=255)
                                                                                           gAATTTAATTAATAGGAACCTATAAAAAAACAGTAAGCCATTTAGATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                                  <  2:17221/141‑1 (MQ=255)
                                                                                           gAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAGTGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                                  >  1:1063026/1‑141 (MQ=255)
                                                                                            aaTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                                 >  1:779005/1‑141 (MQ=255)
                                                                                            aaTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                                 <  2:675038/141‑1 (MQ=255)
                                                                                            aaTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                                 <  2:419618/141‑1 (MQ=255)
                                                                                                  attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                                           >  1:276384/1‑141 (MQ=255)
                                                                                                  attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGGTTTTTGAATGAGGCCAACAAGATGAACGTAGACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTTTAACaataat                                           >  1:488366/1‑141 (MQ=255)
                                                                                                    taatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTg                                         <  2:304186/141‑1 (MQ=255)
                                                                                                    taatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTATTGTTACAACAATTg                                         <  3:39811/141‑1 (MQ=255)
                                                                                                       tAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaa                                      <  1:1030250/141‑1 (MQ=255)
                                                                                                        aGAAACCTATAAAAAAACAGTAATCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaac                                     <  2:22835/141‑1 (MQ=255)
                                                                                                          aaaCCTATAAAAAAACGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGAATGCTAATACAATTGTTGTAACAATAATTGCaacaac                                  >  2:168306/1‑141 (MQ=255)
                                                                                                              cTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTATTTAAAAGAAAACAATAATAATAACGAAAGCGTTTCATCGCTTTGATGATTAAGAGAGAGAGTGCAACTAGAATTGTGGTAAAAATAAATACAaaaacagc                               >  2:497249/1‑139 (MQ=255)
                                                                                                                   aaaaaaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGa                          >  2:775826/1‑141 (MQ=255)
                                                                                                                   aaaaaaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGa                          >  1:299195/1‑141 (MQ=255)
                                                                                                                   aaaaaaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGa                          >  2:1049274/1‑141 (MQ=255)
                                                                                                                   aaaaaaCAGTAAGCCATTTAAATGACTTACTGTCTTTAGAATTAGGCCAACAATATTAACGTATACCTTTCATCGATATGATGATAAAAGGTGAGATTGCCAATACAATTGTTGTAACTATAATTGCAACAACCCCTACGa                          >  2:951850/1‑141 (MQ=255)
                                                                                                                       aaCAGTAATCCAGTTAAATGACTTAATGTTTGTGGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt                      <  2:111718/141‑1 (MQ=255)
                                                                                                                       aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt                      <  1:995022/141‑1 (MQ=255)
                                                                                                                       aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt                      <  1:995127/141‑1 (MQ=255)
                                                                                                                         cAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGAATGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATaa                    <  1:120290/141‑1 (MQ=255)
                                                                                                                          aGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATaaa                   <  4:263959/141‑1 (MQ=255)
                                                                                                                           gTAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg                  >  1:1050222/1‑141 (MQ=255)
                                                                                                                           gTAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg                  >  3:135921/1‑141 (MQ=255)
                                                                                                                           gTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg                  <  2:349016/141‑1 (MQ=255)
                                                                                                                             aagtcATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTa                >  1:288764/5‑141 (MQ=255)
                                                                                                                               gCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATTCCTTTCATCGCTTTTTTTTTTTAATGTGAGATTGCTAATACAATCGTTGTAACAATCATTGCAACAACCCCTCGGAAAATACAGTatt              <  4:127036/141‑3 (MQ=255)
                                                                                                                                   tttAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgt          <  3:193026/141‑1 (MQ=255)
                                                                                                                                     tAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAAtttgttt        >  3:81051/1‑141 (MQ=255)
                                                                                                                                       aaTGACTTACTGTTTTTTGAATTAGGTTAACAATATTAACGTATACTTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAAAAACTAGGAAAATAAAGTAATTTGTTTGa      >  2:892098/1‑141 (MQ=255)
                                                                                                                                       aaTGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGa      >  1:151808/1‑141 (MQ=255)
                                                                                                                                        aTGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGAATGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGAc     <  4:299476/141‑1 (MQ=255)
                                                                                                                                         tGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGAcc    <  2:133929/141‑1 (MQ=255)
                                                                                                                                          gACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCt   <  1:143170/141‑1 (MQ=255)
                                                                                                                                           aCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCTa  <  1:208315/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATTTGTTTGACCTA  >  USA300TCH1516_ALE/803400‑803679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: