BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	841,492	T→G	5.2%	G575G (GGT→GGG)	uvrA →	UvrABC system protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	841,492	0	T	G	5.2%	430.3 / 10.2	143	G575G (GGT→GGG)	uvrA	UvrABC system protein A
Reads supporting (aligned to +/- strand): ref base T (65/70); new base G (2/6); total (67/76)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ATACACTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGCAACAA  >  USA300TCH1516_ALE/841353‑841629

atACACTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTa                                                                                                                                          <  1:1086292/141‑1 (MQ=255)

atACACTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTa                                                                                                                                          <  1:1086479/141‑1 (MQ=255)

 tACACTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTAc                                                                                                                                         <  1:692042/141‑1 (MQ=255)

   cacTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTActc                                                                                                                                       <  2:592134/141‑1 (MQ=255)

     cTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACATAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTActcct                                                                                                                                     >  2:575813/1‑141 (MQ=255)

       tAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCa                                                                                                                                   <  2:328410/141‑1 (MQ=255)

         aaGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCaaa                                                                                                                                 >  1:695519/1‑141 (MQ=255)

          aGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCaaaa                                                                                                                                <  2:448599/141‑1 (MQ=255)

          aGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCaaaa                                                                                                                                <  3:126288/141‑1 (MQ=255)

          aGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCaaaa                                                                                                                                <  1:591571/141‑1 (MQ=255)

           gAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAg                                                                                                                               <  3:331975/141‑1 (MQ=255)

             aaTGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGt                                                                                                                             >  2:547897/1‑141 (MQ=255)

               tGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGCTTGTGTCTAGTGGTACTCCTCAAAAGGTaa                                                                                                                           >  3:48983/1‑141 (MQ=255)

               tGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTaa                                                                                                                           <  2:135183/141‑1 (MQ=255)

               tGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTaa                                                                                                                           >  3:365033/1‑141 (MQ=255)

               tGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTaa                                                                                                                           >  3:139021/1‑141 (MQ=255)

                gagagaTTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAAt                                                                                                                          >  2:599695/1‑141 (MQ=255)

                   agaTTTAGGAAATACTTTAATTTTACTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCGGGTGAACATGGAGTGCAGATTGTGGCTAGTGGTACTCCTCAAAAGGTAATGaa                                                                                                                       <  2:346721/141‑1 (MQ=255)

                     aTTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAg                                                                                                                     >  2:928096/1‑141 (MQ=255)

                      tttAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGa                                                                                                                    <  3:223234/141‑1 (MQ=255)

                       ttAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGAt                                                                                                                   >  3:319522/1‑141 (MQ=255)

                         aGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATaa                                                                                                                 >  2:300120/1‑141 (MQ=255)

                          ggAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATaaa                                                                                                                >  4:167329/1‑141 (MQ=255)

                            aaaTACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTCCTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATaaaaa                                                                                                              >  1:181818/1‑141 (MQ=255)

                            aaaTACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATaaaaa                                                                                                              >  1:574707/1‑141 (MQ=255)

                             aaTACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATaaaaaa                                                                                                             <  3:256659/141‑1 (MQ=255)

                              aTACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAACGATAAAAAAt                                                                                                            >  3:19566/1‑141 (MQ=255)

                                aCTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCa                                                                                                          <  2:779104/141‑1 (MQ=255)

                                aCTTTAATTGTAGCTGAACACGATGAGGATACCATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAGCATGGAGGGCAGATCGTGTATAGTGGTACGCCTCAAAAGGTATAGAAAGATAAAAAATCa                                                                                                          <  4:273949/141‑1 (MQ=255)

                                          tAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGAc                                                                                                <  1:182286/141‑1 (MQ=255)

                                          tAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGAc                                                                                                <  3:43514/141‑1 (MQ=255)

                                            gTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGGTGGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCAGTAACAGGACaa                                                                                              >  2:822494/1‑141 (MQ=255)

                                             ttGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAAt                                                                                             <  3:51363/141‑1 (MQ=255)

                                             ttGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGGCCTGGTGCTGGTGAACATGGAGGACAAATTGTGTCTAGTGGGACACCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAAt                                                                                             <  2:335791/141‑1 (MQ=255)

                                              tGAACACTATGATGATACAAGGCGTGCGGCGGAGTACTTAGTGGATATAGGGCCTGGTGCTGTTGTACATGGATGTCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATa                                                                                            <  1:593450/141‑1 (MQ=255)

                                               gAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATAc                                                                                           >  3:247914/1‑141 (MQ=255)

                                                    agatgatgatACAATGCGTGCGGCGGATTACATAGAGGATATAGGTCCTGGTGCTGGTGAACATGGAGAGCAGATTGTATCTAGTGGTACTCCTCAAAAGGTAAAAAAAAAAAAAAAATCATTAACAGGACAATACTTGAg                                                                                      <  2:871463/140‑1 (MQ=255)

                                                     gatgatgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGt                                                                                     >  2:347023/1‑141 (MQ=255)

                                                     gatgatgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGt                                                                                     >  2:194224/1‑141 (MQ=255)

                                                      atgatgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTg                                                                                    >  1:846473/1‑141 (MQ=255)

                                                      atgatgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGGACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTg                                                                                    >  1:1053294/1‑141 (MQ=255)

                                                        gatgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGt                                                                                  <  2:999005/141‑1 (MQ=255)

                                                         atgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTa                                                                                 <  2:381285/141‑1 (MQ=255)

                                                         atgatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTa                                                                                 >  4:253422/1‑141 (MQ=255)

                                                         atgagACAAGGCTGGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGGACGCCGCAGTATGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTa                                                                                 <  2:762642/141‑1 (MQ=255)

                                                           gatACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAg                                                                               >  2:77552/1‑141 (MQ=255)

                                                             tacgctgcgtgggGCTGAGTACTAAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGCTACTCCTCACAAAGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTACGaa                                                                             <  2:376422/136‑1 (MQ=255)

                                                             tACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGaa                                                                             <  1:463226/141‑1 (MQ=255)

                                                               cAATGCCTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAAc                                                                           >  4:375533/1‑141 (MQ=255)

                                                                  tgcgtgcgGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTa                                                                        >  2:677967/1‑141 (MQ=255)

                                                                  tgcgtgcgGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTa                                                                        >  1:142057/1‑141 (MQ=255)

                                                                    cgtgcgGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTAtt                                                                      >  1:747030/1‑141 (MQ=255)

                                                                    cgtgcgGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTAtt                                                                      <  1:58103/141‑1 (MQ=255)

                                                                       gcgGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGaa                                                                   >  4:109301/1‑141 (MQ=255)

                                                                            tGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTAcc                                                              >  4:419034/1‑141 (MQ=255)

                                                                                  cTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAata                                                        >  1:225666/1‑141 (MQ=255)

                                                                                  cTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAata                                                        <  3:77288/141‑1 (MQ=255)

                                                                                      gTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGc                                                    <  2:990551/141‑1 (MQ=255)

                                                                                       tGGGTAGAGGTGCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAAAAAAAAAAAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCa                                                   <  4:297497/141‑1 (MQ=255)

                                                                                        ggATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAg                                                  <  4:148400/141‑1 (MQ=255)

                                                                                           tataGGTCCTGGTGCTGGTGAACATTGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGAcc                                               >  2:20235/1‑141 (MQ=255)

                                                                                           tataGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGAcc                                               >  1:698893/1‑141 (MQ=255)

                                                                                            ataGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGaa                                                                             <  1:230046/110‑1 (MQ=255)

                                                                                            ataGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGaa                                                                             >  2:230108/1‑110 (MQ=255)

                                                                                            ataGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGaa                                                                             >  2:230046/1‑110 (MQ=255)

                                                                                            ataGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCg                                              >  1:704613/1‑141 (MQ=255)

                                                                                             taGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCgg                                             <  4:20844/141‑1 (MQ=255)

                                                                                              aGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGc                                            >  1:665321/1‑141 (MQ=255)

                                                                                                 tCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGGGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGAAAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTc                                         <  1:381189/141‑1 (MQ=255)

                                                                                                  ccTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCa                                        >  2:484970/1‑141 (MQ=255)

                                                                                                  ccTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCa                                        >  2:838352/1‑141 (MQ=255)

                                                                                                   ctggtgctggtgAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGAGATGAAAGATAAAAAATCATTAACAGGACAATACTTTAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAg                                       <  1:318523/141‑1 (MQ=255)

                                                                                                    tggtgctggtgAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGa                                      >  3:71493/1‑141 (MQ=255)

                                                                                                      gtgctggtgAACATGGAGGACAAATTGTGTCTAGTGGGACACCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGACGTACCTGAATATCGCAGACCGGCTTCAGATc                                    <  2:547407/141‑1 (MQ=255)

                                                                                                       tgctggtgAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCg                                   <  4:398995/141‑1 (MQ=255)

                                                                                                       tgctggtgAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCg                                   <  4:240342/141‑1 (MQ=255)

                                                                                                       tgctggtgAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCg                                   <  3:185732/141‑1 (MQ=255)

                                                                                                       gggttgtgAACATGGAGGGCAGATTGTGTATAGTGGTGATCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCg                                   <  2:706909/136‑1 (MQ=255)

                                                                                                           ggtgAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTaaa                               >  1:46443/1‑141 (MQ=255)

                                                                                                              gAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAAtt                            <  1:547897/141‑1 (MQ=255)

                                                                                                               aaCATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAAttt                           >  2:1063896/1‑141 (MQ=255)

                                                                                                                aCATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTc                          <  2:861908/141‑1 (MQ=255)

                                                                                                                  aTGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCta                        <  1:534155/141‑1 (MQ=255)

                                                                                                                  aTGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCta                        <  1:841517/141‑1 (MQ=255)

                                                                                                                  aTGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCta                        <  2:3867/141‑1 (MQ=255)

                                                                                                                    ggAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCtata                      >  1:629642/1‑141 (MQ=255)

                                                                                                                     gAGGACAAATTGTGTCTAGTGGGACACCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGACGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATAc                     >  1:202345/1‑141 (MQ=255)

                                                                                                                        ggCAGAGTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTg                  <  2:1066910/141‑1 (MQ=255)

                                                                                                                        gacaaaTTGTGTCTAGTGGGACACCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGACGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTg                  <  1:91980/136‑1 (MQ=255)

                                                                                                                            gATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGc              <  4:319522/141‑1 (MQ=255)

                                                                                                                            gATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCGTCAGATCGTAAAATTTCTATACGTGGAGc              <  2:41064/141‑1 (MQ=255)

                                                                                                                             aTTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCt             >  1:114501/1‑141 (MQ=255)

                                                                                                                             aTTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCt             >  1:66648/1‑141 (MQ=255)

                                                                                                                             aTTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCt             >  2:618434/1‑141 (MQ=255)

                                                                                                                              ttGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTa            >  2:625875/1‑141 (MQ=255)

                                                                                                                                gtgtCTAGTGGGACACCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGACGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGa          <  1:779356/141‑1 (MQ=255)

                                                                                                                                    cTAGTGGTACTCCCCAAAAGGTAATGAAAGATAAAAAACCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGca      <  1:734706/141‑1 (MQ=255)

                                                                                                                                      aGTGGTTCTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGcaac    <  1:571849/141‑1 (MQ=255)

                                                                                                                                      aGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGcaac    >  1:916607/1‑141 (MQ=255)

                                                                                                                                        tGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGcaacaa  <  2:374757/141‑1 (MQ=255)

ATACACTTAAAGAAATGAGAGATTTAGGAAATACTTTAATTGTAGTTGAACACGATGATGATACAATGCGTGCGGCTGATTACTTAGTGGATATAGGTCCTGGTGCTGGTGAACATGGAGGGCAGATTGTGTCTAGTGGTACTCCTCAAAAGGTAATGAAAGATAAAAAATCATTAACAGGACAATACTTGAGTGGTAAGAAACGTATTGAAGTACCTGAATATCGCAGACCGGCTTCAGATCGTAAAATTTCTATACGTGGAGCTAGAAGCAACAA  >  USA300TCH1516_ALE/841353‑841629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`