Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 853,045 A→G 5.1% intergenic (+52/+191) clpP_1 → / ← USA300HOU_RS04170 ATP‑dependent Clp protease proteolytic subunit/Epimerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE853,0450AG 5.1% 456.8 / 9.3 151intergenic (+52/+191)clpP_1/USA300HOU_RS04170ATP‑dependent Clp protease proteolytic subunit/Epimerase family protein
Reads supporting (aligned to +/- strand):  ref base A (69/74);  new base G (4/4);  total (73/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

ACACAGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAG  >  USA300TCH1516_ALE/852908‑853185
                                                                                                                                         |                                                                                                                                            
   caGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCGTTTTGTATGAGTAAACCAAGgtgt                                                                                                                                        <  1:261554/141‑1 (MQ=255)
   caGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCATAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTACAGAGCCAACTGAGCTGTCTGCTATTTTTGTATGAGTAAACGAAGgtgt                                                                                                                                        >  1:10261/1‑141 (MQ=255)
    aGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTc                                                                                                                                       <  2:330764/141‑1 (MQ=255)
    aGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTc                                                                                                                                       >  2:753912/1‑141 (MQ=255)
     gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa                                                                                                                                      >  2:1003195/1‑141 (MQ=255)
     gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa                                                                                                                                      <  1:820871/141‑1 (MQ=255)
     gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa                                                                                                                                      <  3:387141/141‑1 (MQ=255)
     gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa                                                                                                                                      <  4:110569/141‑1 (MQ=255)
     gATCGTGATAACTTCTTAACTGCAGAAGAAACTAAAGAATATGGCTTAATTGACGAAGTGATGGTACTTGAAACAAAATAATTAAAAGTAAAGAGTAGACTACGCTGTCTGCACTTTTTGTATGAGTAAACGAAGGTGTCa                                                                                                                                      >  4:234765/1‑141 (MQ=255)
        cGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCaata                                                                                                                                   >  1:641070/1‑141 (MQ=255)
          tGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCaataat                                                                                                                                 <  3:114274/141‑1 (MQ=255)
           gATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAAtt                                                                                                                                >  3:17290/1‑141 (MQ=255)
                  tcttAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTAc                                                                                                                         >  1:128832/1‑141 (MQ=255)
                   cttAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACt                                                                                                                        <  2:504305/141‑1 (MQ=255)
                   cttAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACt                                                                                                                        >  2:724271/1‑141 (MQ=255)
                   cttAACTGCAGAAGAAGCTAAAGAATATGGCCTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCGTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACt                                                                                                                        >  3:111614/1‑141 (MQ=255)
                          gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt                                                                                                                 >  4:220757/1‑141 (MQ=255)
                          gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt                                                                                                                 >  3:50601/1‑141 (MQ=255)
                          gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt                                                                                                                 >  1:80504/1‑141 (MQ=255)
                          gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt                                                                                                                 >  3:246608/1‑141 (MQ=255)
                           cAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTg                                                                                                                <  4:304632/141‑1 (MQ=255)
                            agaagaAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGa                                                                                                               >  1:601307/1‑141 (MQ=255)
                              aagaagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGc                                                                                                             >  4:368727/1‑141 (MQ=255)
                              aagaagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGc                                                                                                             >  1:977386/1‑141 (MQ=255)
                                 aagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGa                                                                                                          <  2:508083/141‑1 (MQ=255)
                                 aagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCAGTCTGCTCTTTTTGTATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTCGAGCGGa                                                                                                          <  3:402135/141‑1 (MQ=255)
                                    cTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAat                                                                                                       <  2:79046/141‑1 (MQ=255)
                                     tAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAata                                                                                                      <  2:152297/141‑1 (MQ=255)
                                     gaaaGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAata                                                                                                      <  2:968887/140‑1 (MQ=255)
                                         gAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGAt                                                                                                  <  2:512367/141‑1 (MQ=255)
                                          aaTATGGCTTAATTGATGAAGTGATGGTACCCGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGAtt                                                                                                 >  1:271387/1‑141 (MQ=255)
                                             atGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaa                                                                                              >  1:413432/1‑141 (MQ=255)
                                              tGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaac                                                                                             <  3:273712/141‑2 (MQ=255)
                                               ggCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatg                                                                                            >  1:552331/1‑141 (MQ=255)
                                                gCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatga                                                                                           >  1:560565/1‑141 (MQ=255)
                                                   tAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGc                                                                                        <  2:1051452/141‑1 (MQ=255)
                                                        gatgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGtt                                                                                   <  1:701115/141‑1 (MQ=255)
                                                         atgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTg                                                                                  >  1:475706/1‑141 (MQ=255)
                                                         atgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTg                                                                                  >  1:98013/1‑141 (MQ=255)
                                                          tgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGa                                                                                 >  1:1047094/1‑141 (MQ=255)
                                                          tgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGa                                                                                 <  1:510088/141‑1 (MQ=255)
                                                          tgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGa                                                                                 >  2:556774/1‑141 (MQ=255)
                                                           gaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGaa                                                                                >  2:606212/1‑141 (MQ=255)
                                                           gaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGaa                                                                                >  1:898699/1‑141 (MQ=255)
                                                                  tGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAAc                                                                         <  2:4590/141‑1 (MQ=255)
                                                                  tGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAAc                                                                         <  1:279689/141‑1 (MQ=255)
                                                                     tACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACtat                                                                      >  2:580581/1‑141 (MQ=255)
                                                                     tACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACtat                                                                      <  1:614303/141‑1 (MQ=255)
                                                                      aCCTGAAATAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACtata                                                                     >  2:525578/1‑141 (MQ=255)
                                                                         tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa                                                                  <  2:315545/141‑1 (MQ=255)
                                                                         tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa                                                                  <  1:358846/141‑1 (MQ=255)
                                                                         tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa                                                                  <  3:191216/141‑1 (MQ=255)
                                                                         tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa                                                                  <  4:113390/141‑1 (MQ=255)
                                                                         tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa                                                                  <  3:369344/141‑1 (MQ=255)
                                                                          gAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGaa                                                                 <  1:755445/141‑1 (MQ=255)
                                                                           aaaCAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGaaa                                                                <  1:199934/141‑1 (MQ=255)
                                                                            aaCAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatg                                                                                            >  1:590401/1‑112 (MQ=255)
                                                                            aaCAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatg                                                                                            <  2:590401/112‑1 (MQ=255)
                                                                             aCAAAATAATTCAAAGTAAAGAGTAGACTAAGCAGTCTGCTCTTTTTGTATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATg                                                              >  4:425015/1‑141 (MQ=255)
                                                                              cAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGt                                                             >  1:750066/1‑141 (MQ=255)
                                                                                aaaTAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTCACTATATGAAATGTTc                                                           >  3:324783/1‑141 (MQ=255)
                                                                                        cAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCaaa                                                   <  2:833388/141‑1 (MQ=255)
                                                                                            gTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAg                                               <  1:343663/141‑1 (MQ=255)
                                                                                            gTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAg                                               <  4:276109/141‑1 (MQ=255)
                                                                                             tAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGa                                              <  1:183230/141‑1 (MQ=255)
                                                                                               aaGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACa                                            >  1:512847/1‑141 (MQ=255)
                                                                                                agagTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCGAGGTGTTAATAATTTGTTTACTATATTTTGAGCGGCAATATGATTGAATGAAGCTAGTTGAACCCTAACTGTTTTAGATGTTACCTTCTAAGTAGCCAt                                           >  2:286980/1‑141 (MQ=255)
                                                                                                agagTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACAt                                           <  2:437810/141‑1 (MQ=255)
                                                                                                 gagTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAAGTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTATACCtt                                          >  1:141612/1‑141 (MQ=255)
                                                                                                  agTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTg                                         <  2:244567/141‑1 (MQ=255)
                                                                                                       aCTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAgg                                    <  1:455020/141‑1 (MQ=255)
                                                                                                        cTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGa                                   >  1:489920/1‑141 (MQ=255)
                                                                                                            gCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGaaatgttcct                               <  1:1040050/141‑8 (MQ=255)
                                                                                                               gTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTc                            <  2:315847/141‑1 (MQ=255)
                                                                                                                tCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCa                           <  3:226409/141‑1 (MQ=255)
                                                                                                                   gCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATc                        <  2:1010970/141‑1 (MQ=255)
                                                                                                                     actTTTTTTATTAGTAAACTAAGTAGTCAATAATATATTTATTATACTTTGTGCGGAAAGATGAAGTAATGCATCTAGTTGAACCGTAACTATTTGAAATATTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCt                      >  2:687005/2‑141 (MQ=255)
                                                                                                                      ctTTTTGTATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtt                     <  1:216284/141‑1 (MQ=255)
                                                                                                                        ttttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttg                   <  4:61005/141‑1 (MQ=255)
                                                                                                                        ttttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttg                   <  2:1064669/141‑1 (MQ=255)
                                                                                                                        gtttGTGTGAGTACACCAAGGTAACAATCCTTCGTTTACTCTACTTTGAGCGGGAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGACGGTAACATTTCAATCCtttg                   >  2:517643/2‑141 (MQ=255)
                                                                                                                         tttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttgt                  >  1:944682/1‑141 (MQ=255)
                                                                                                                          ttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttgtt                 <  1:134010/141‑1 (MQ=255)
                                                                                                                           tGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCTTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttgttt                <  2:267691/141‑1 (MQ=255)
                                                                                                                             tATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTttgtttgt              <  2:958606/141‑1 (MQ=255)
                                                                                                                             tATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTttgtttgt              >  2:172426/1‑141 (MQ=255)
                                                                                                                             tATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTttgtttgt              >  1:1052244/1‑141 (MQ=255)
                                                                                                                              aTGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTa             >  3:299048/1‑141 (MQ=255)
                                                                                                                              aTGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTa             >  1:512837/1‑141 (MQ=255)
                                                                                                                               tGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTaa            <  1:263047/141‑1 (MQ=255)
                                                                                                                               tGAGTAAACCAAGCTGTCAATAATTTGTTTTCTCTACTTTTAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGACATGTTCCCTTCAAAGTATACATTGAAAGGAACATTTCAATCCTTTGTTTGCaa            >  2:864857/1‑141 (MQ=255)
                                                                                                                                gAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAg           <  2:896163/141‑1 (MQ=255)
                                                                                                                                gAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAg           >  2:415250/1‑141 (MQ=255)
                                                                                                                                   tAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCg        >  1:1011462/1‑141 (MQ=255)
                                                                                                                                   tAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCg        >  2:339760/1‑141 (MQ=255)
                                                                                                                                   tAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTGGTTTGTAAGTCg        >  1:147668/1‑141 (MQ=255)
                                                                                                                                    aaaCCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGc       <  2:977386/141‑1 (MQ=255)
                                                                                                                                        cAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTa   >  3:248581/1‑141 (MQ=255)
                                                                                                                                         aaGGTGTCAATAATTTGTTTACTATACTTTGAGCGGCACTAGGGTTTAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAg  >  1:1062792/1‑141 (MQ=255)
                                                                                                                                         aaGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAg  >  1:806391/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
ACACAGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAG  >  USA300TCH1516_ALE/852908‑853185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: