Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 895,189 A→G 7.2% T70T (ACA→ACG metQ_2 → Methionine‑binding lipoprotein MetQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE895,1890AG 7.2% 315.9 / 11.8 111T70T (ACA→ACGmetQ_2Methionine‑binding lipoprotein MetQ
Reads supporting (aligned to +/- strand):  ref base A (51/52);  new base G (5/3);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 118 total aligned reads displayed.

AAAAGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACT  >  USA300TCH1516_ALE/895052‑895328
                                                                                                                                         |                                                                                                                                           
aaaaGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAACAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCt                                                                                                                                          <  1:816502/141‑1 (MQ=255)
aaaaGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCt                                                                                                                                          <  1:806356/141‑1 (MQ=255)
 aaaGTGGCAGTGACGACAAGTAAATATCTGTAGGTGCTTCACCAGTACCACATGCTAAAATTGTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCtg                                                                                                                                         <  1:1033260/141‑2 (MQ=255)
   aGTGGCAGTGACGACAAGACAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATAAAAACAATTAACGATTACACTACACCtaaa                                                                                                                                       >  2:236569/1‑140 (MQ=255)
   aGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCtaat                                                                                                                                       <  2:425120/141‑1 (MQ=255)
   aGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCtaat                                                                                                                                       >  1:1076663/1‑141 (MQ=255)
    gTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCtaata                                                                                                                                      >  2:17805/1‑141 (MQ=255)
    gTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCtaata                                                                                                                                      >  1:36008/1‑141 (MQ=255)
         aGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAGAGTTTAGAAAAGGCAAAACCATTATTAGAGAAAAAAGGGTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTa                                                                                                                                 >  3:409907/1‑141 (MQ=255)
           tGACGACAAGAAAATAACAGTAGGTGCTTCACCAGAACCACATGAAAAAATTATAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACt                                                                                                                               <  2:9907/141‑1 (MQ=255)
            gacgacAAGAAAATAACAGTAGGTGCTTCACTAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTACAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTa                                                                                                                              <  1:692946/141‑1 (MQ=255)
                 cAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACaa                                                                                                                         <  1:1025308/141‑1 (MQ=255)
                    gAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACAAAAAAAAAAAAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAgg                                                                                                                      <  4:33711/141‑1 (MQ=255)
                    gAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACAAAAAAAAAAAAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAgg                                                                                                                      <  4:33719/141‑1 (MQ=255)
                     aaaaTAACGGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAGAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACCATTAACGATTACACTACACCTAATAAATTACTAGACGAAGGt                                                                                                                     <  2:509884/141‑1 (MQ=255)
                          aaCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAt                                                                                                                <  4:30417/141‑1 (MQ=255)
                          aaCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAt                                                                                                                <  3:300013/141‑1 (MQ=255)
                          aaCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAt                                                                                                                >  2:56706/1‑141 (MQ=255)
                           aCAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAAtt                                                                                                               >  3:186207/1‑141 (MQ=255)
                             aGTAGGTGCTTCACCAGCACCACATGGTGAAATTTTAGAAAAAGCAAAACCATTATCAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGa                                                                                                             >  4:24345/1‑141 (MQ=255)
                             aGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGa                                                                                                             >  4:110655/1‑141 (MQ=255)
                              gTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGAc                                                                                                            >  1:553908/1‑141 (MQ=255)
                                aGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGc                                                                                                          >  2:375263/1‑141 (MQ=255)
                                aGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGc                                                                                                          >  1:817786/1‑141 (MQ=255)
                                aGGTGCTTCACCAGCACCACATGCCGAAATTTTAGAAAAAGCAAAACCAATAAAAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGc                                                                                                          <  2:917992/141‑1 (MQ=255)
                                   tGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCaaa                                                                                                       >  1:28181/1‑141 (MQ=255)
                                   tGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCaaa                                                                                                       >  3:328421/1‑141 (MQ=255)
                                     cTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACt                                                                                                     <  1:502612/141‑1 (MQ=255)
                                      ttCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTa                                                                                                    >  2:871248/1‑141 (MQ=255)
                                      tggtgaaGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTa                                                                                                    >  2:290776/7‑141 (MQ=255)
                                        cACCAGCACCACATGCTGAAATTTTAGAAAAAGCACAACCATTATTAGAGCAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTAtt                                                                                                  <  1:377492/141‑1 (MQ=255)
                                        cACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTAtt                                                                                                  <  1:997443/141‑1 (MQ=255)
                                         aCCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTAttt                                                                                                 <  2:211577/141‑1 (MQ=255)
                                           cAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTcc                                                                                               <  3:427431/141‑1 (MQ=255)
                                              caccacATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAAc                                                                                            >  4:262958/1‑141 (MQ=255)
                                                ccacATGCTGAAATATTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATGGACGCAAACTATTTCCAACAt                                                                                          >  2:662716/1‑141 (MQ=255)
                                                  acaTGCTGGAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATac                                                                                        <  2:332968/141‑1 (MQ=255)
                                                    aTGCTGANATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATacac                                                                                      <  2:812329/141‑1 (MQ=255)
                                                    aTGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATacac                                                                                      >  1:713503/1‑141 (MQ=255)
                                                    aTGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATacac                                                                                      >  2:390807/1‑141 (MQ=255)
                                                     tGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACAcc                                                                                     <  1:990264/141‑1 (MQ=255)
                                                     tGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACAcc                                                                                     <  2:917191/141‑1 (MQ=255)
                                                          aaaTTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATAtt                                                                                >  1:949063/1‑141 (MQ=255)
                                                          aaaTTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATAtt                                                                                >  3:441071/1‑141 (MQ=255)
                                                               ttAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAac                                                                           >  4:279567/1‑141 (MQ=255)
                                                               ttAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAac                                                                           <  4:200483/141‑1 (MQ=255)
                                                                tAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAaca                                                                          >  3:299649/1‑141 (MQ=255)
                                                                  gAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAAcaca                                                                        >  1:394928/1‑141 (MQ=255)
                                                                   aaaaaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACag                                                                       <  3:308301/141‑1 (MQ=255)
                                                                   aaaaaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACag                                                                       <  3:188166/141‑1 (MQ=255)
                                                                   aaaaaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACag                                                                       <  3:88495/141‑1 (MQ=255)
                                                                      aaGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAgaga                                                                    >  3:21271/1‑141 (MQ=255)
                                                                       aGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGaa                                                                   >  4:133473/1‑141 (MQ=255)
                                                                         cAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGaaaa                                                                 <  1:387809/141‑1 (MQ=255)
                                                                            aaCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAgg                                                              >  2:1069994/1‑141 (MQ=255)
                                                                            aaCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAgg                                                              >  1:718702/1‑141 (MQ=255)
                                                                             aCCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGa                                                             <  1:252818/141‑1 (MQ=255)
                                                                                 ttattaGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATaaa                                                         >  2:610548/1‑141 (MQ=255)
                                                                                      agagAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCa                                                                                    >  3:7384/1‑109 (MQ=255)
                                                                                      agagAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTa                                                    >  2:865/1‑141 (MQ=255)
                                                                                        agaAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACa                                                  <  3:23794/141‑1 (MQ=255)
                                                                                        agaAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACAATACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAACAAGGATCAAGGTTACa                                                  >  2:574318/1‑141 (MQ=255)
                                                                                            aaaaGGTTATGAACTAGATATTAAAACAGTTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTACCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAAt                                              <  1:72457/141‑1 (MQ=255)
                                                                                               aGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGt                                           <  1:865217/141‑1 (MQ=255)
                                                                                               aGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAAAAAAAAAAAAAAGGATAAAGGTTACAAAATCGt                                           <  4:380223/141‑1 (MQ=255)
                                                                                                  ttATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAg                                        <  3:292475/141‑1 (MQ=255)
                                                                                                  ttATGAACGAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAg                                        <  4:365035/141‑1 (MQ=255)
                                                                                                   tATGACCTAGATATTAAAACAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGt                                       >  3:164807/1‑141 (MQ=255)
                                                                                                   tATGAACTAGATATTAAAACAATTAACGATAACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGt                                       >  2:975331/1‑141 (MQ=255)
                                                                                                     tGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGc                                     >  1:438386/1‑141 (MQ=255)
                                                                                                     tGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGc                                     >  2:315940/1‑141 (MQ=255)
                                                                                                     tGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGc                                     >  2:1055572/1‑141 (MQ=255)
                                                                                                         cTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGt                                 <  3:145408/141‑1 (MQ=255)
                                                                                                            gATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGAt                              <  1:611499/141‑1 (MQ=255)
                                                                                                             atatTAACACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATg                             <  3:411292/141‑1 (MQ=255)
                                                                                                              tatTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGt                            <  4:369201/141‑1 (MQ=255)
                                                                                                                ttAAAACAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTc                          <  2:773829/141‑1 (MQ=255)
                                                                                                                ttAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTc                          <  2:414736/141‑1 (MQ=255)
                                                                                                                ttAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTc                          <  1:510878/141‑1 (MQ=255)
                                                                                                                ttAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTc                          <  1:849316/141‑1 (MQ=255)
                                                                                                                  aaaaCAATTAACGATTACACTACGCCTAATAAATTACTAGATAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCAc                        >  1:266964/1‑141 (MQ=255)
                                                                                                                   aaaCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACt                       >  1:209585/1‑141 (MQ=255)
                                                                                                                     aCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTa                     >  2:9999/1‑141 (MQ=255)
                                                                                                                     aCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTa                     >  1:51428/1‑141 (MQ=255)
                                                                                                                     aCAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTa                     >  1:123474/1‑141 (MQ=255)
                                                                                                                      cAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACAAAGAAAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAg                    <  4:271057/141‑1 (MQ=255)
                                                                                                                       aaTTAACGATTACACTACACCTAATAAATTACTAGACAAAGGt                                                                                                                     <  1:162888/43‑1 (MQ=255)
                                                                                                                        aTTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGaa                  >  4:132662/1‑141 (MQ=255)
                                                                                                                          tAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAAcc                >  2:503211/1‑141 (MQ=255)
                                                                                                                          tAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAAcc                <  2:876392/141‑1 (MQ=255)
                                                                                                                              gATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATg            <  1:724728/141‑1 (MQ=255)
                                                                                                                               aTTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATgg           <  1:1055572/141‑1 (MQ=255)
                                                                                                                               aTTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATgg           <  1:85251/141‑1 (MQ=255)
                                                                                                                                  acactacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTg        >  2:390344/1‑141 (MQ=255)
                                                                                                                                    actacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGta      <  1:386990/141‑1 (MQ=255)
                                                                                                                                      tacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGTCGGTGATGTTCACTTAGAACCTATGGCTGtata    <  1:564157/141‑1 (MQ=255)
                                                                                                                                      tacacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGtata    <  1:325337/141‑1 (MQ=255)
                                                                                                                                       acacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTATAACCTATGGCTGTATAc   <  3:449869/141‑1 (MQ=255)
                                                                                                                                       acacCTAATAAATTACTAGACAAAGGTGAAACTGACGCAAACTATTTCCAACATACACCATATTTAAAAACAGAGAAAAAGGATAAGGTTACAACATCGTAAGTGCCGGTGATGTTCACTCAGCAACTATGGCTGTATAct  >  2:702337/1‑141 (MQ=255)
                                                                                                                                        cacCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATAct  <  4:136514/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AAAAGTGGCAGTGACGACAAGAAAATAACAGTAGGTGCTTCACCAGCACCACATGCTGAAATTTTAGAAAAAGCAAAACCATTATTAGAGAAAAAAGGTTATGAACTAGATATTAAAACAATTAACGATTACACTACACCTAATAAATTACTAGACAAAGGTGAAATTGACGCAAACTATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACT  >  USA300TCH1516_ALE/895052‑895328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: