Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 895,375 C→T 5.5% V132V (GTC→GTT metQ_2 → Methionine‑binding lipoprotein MetQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE895,3750CT 5.5% 387.3 / 7.7 127V132V (GTC→GTTmetQ_2Methionine‑binding lipoprotein MetQ
Reads supporting (aligned to +/- strand):  ref base C (72/47);  new base T (4/3);  total (77/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 128 total aligned reads displayed.

CAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATT  >  USA300TCH1516_ALE/895235‑895510
                                                                                                                                            |                                                                                                                                       
cAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTc                                                                                                                                         >  1:131139/1‑141 (MQ=255)
    aTACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTAtg                                                                                                                                     >  4:22938/1‑141 (MQ=255)
      acacCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTAtgtg                                                                                                                                   >  2:988394/1‑141 (MQ=255)
       cacCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGTTGCAACAGTCTATgtgt                                                                                                                                  <  2:594710/141‑1 (MQ=255)
       cacCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATgtgt                                                                                                                                  >  1:25271/1‑141 (MQ=255)
               ttAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTaataat                                                                                                                          >  1:1084786/1‑141 (MQ=255)
               ttAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTaataat                                                                                                                          >  3:12390/1‑141 (MQ=255)
                tAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGATTAAAAGAATTACAAAAAGGTGCAACAATATATGTGTCTaataata                                                                                                                         >  4:415506/1‑140 (MQ=255)
                   acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                      >  2:439336/1‑141 (MQ=255)
                   acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                      >  1:441827/1‑141 (MQ=255)
                   acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                      >  3:317587/1‑141 (MQ=255)
                     acaGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCt                                                                                                                    >  4:351106/1‑141 (MQ=255)
                        gagaAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGaa                                                                                                                 >  2:472189/1‑141 (MQ=255)
                          gaAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACa                                                                                                               >  3:400536/1‑141 (MQ=255)
                            aaaaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAg                                                                                                             <  3:252140/141‑1 (MQ=255)
                            aaaaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTATAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAg                                                                                                             >  4:303441/1‑141 (MQ=255)
                              aaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGa                                                                                                           >  3:273355/1‑141 (MQ=255)
                               aGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGAc                                                                                                          <  2:306431/141‑1 (MQ=255)
                                ggATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACg                                                                                                         >  3:190815/1‑141 (MQ=255)
                                 gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                        <  2:150477/141‑1 (MQ=255)
                                 gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                        <  1:975331/141‑1 (MQ=255)
                                  aTAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGtt                                                                                                       >  1:692687/1‑141 (MQ=255)
                                   tAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGttt                                                                                                      <  4:402945/141‑1 (MQ=255)
                                      aGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTCTCtt                                                                                                   >  2:1022750/1‑141 (MQ=255)
                                         ttACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaa                                                                                                <  2:1049500/141‑1 (MQ=255)
                                         ttACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaa                                                                                                >  1:156122/1‑141 (MQ=255)
                                          tACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaaa                                                                                               >  4:6759/1‑141 (MQ=255)
                                          tACAAAATCGAAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCCAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaaa                                                                                               <  4:409907/141‑1 (MQ=255)
                                           aCAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAt                                                                                              <  1:348061/141‑1 (MQ=255)
                                            cAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAtt                                                                                             <  3:262958/141‑1 (MQ=255)
                                               aaTCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAttctt                                                                                          >  4:250376/1‑141 (MQ=255)
                                                 tCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCg                                                                                        <  3:133473/141‑1 (MQ=255)
                                                 tCGTAAGTGCCGGTGAGGTTCACTAAGAACCTATGGCTGTATACGCTAAAAAGTATGAAAGGTTAAGAGAATTACCAAAAGGTGCAACGGTCTATGTGTCTAATAATCCAGCAGAACAAGGACGTTTCTTAAAATTCTTCg                                                                                        >  2:187703/1‑141 (MQ=255)
                                                  cGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGt                                                                                       <  4:186207/141‑1 (MQ=255)
                                                   gTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGtt                                                                                      >  1:523523/1‑141 (MQ=255)
                                                      aGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGAt                                                                                   >  4:10722/1‑141 (MQ=255)
                                                       gTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATg                                                                                  <  2:503296/141‑1 (MQ=255)
                                                       gTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATg                                                                                  <  4:328421/141‑1 (MQ=255)
                                                            ggTGCTGTTCAATTAGCACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGGATTTCCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGACCAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGt                                                                             <  2:28181/141‑1 (MQ=255)
                                                            ggTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGt                                                                             <  1:290776/141‑1 (MQ=255)
                                                             gTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGtt                                                                            >  2:1041287/1‑141 (MQ=255)
                                                             gTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGtt                                                                            <  1:375263/141‑1 (MQ=255)
                                                              tGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGttt                                                                           <  3:110655/141‑1 (MQ=255)
                                                              tGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGttt                                                                           <  1:390807/141‑1 (MQ=255)
                                                               gATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTtta                                                                          >  2:46274/1‑141 (MQ=255)
                                                                 tGTTGACGTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAGTCTTCGTTGATGCAGGTttaat                                                                        <  2:553772/141‑1 (MQ=255)
                                                                     cACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTaaa                                                                    >  2:260025/1‑141 (MQ=255)
                                                                     cACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTaaa                                                                    <  3:301387/141‑1 (MQ=255)
                                                                      aCTTAGAACCTTTGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGATGATGCAGGTTTAATTaaaa                                                                   >  1:218709/1‑141 (MQ=255)
                                                                      aCTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTttaantaaga                                                                   >  3:161075/1‑139 (MQ=255)
                                                                       cTTAGAACCTATGGCTGTATACTCTACAAAGTATACAAGTTTAAAAGAATTACCAAAAGGCGCCACAGCCTATGTGTCTACTAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGATTACTTAAAAt                                                                  >  4:295830/1‑141 (MQ=255)
                                                                       cTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAAt                                                                  >  2:607189/1‑141 (MQ=255)
                                                                        ttAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATc                                                                 <  1:653388/141‑1 (MQ=255)
                                                                        ttAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATc                                                                 <  1:902916/141‑1 (MQ=255)
                                                                           gAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaa                                                              >  3:454473/1‑141 (MQ=255)
                                                                             aCCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaaaa                                                            >  2:376171/1‑141 (MQ=255)
                                                                             aCCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaaaa                                                            >  3:253703/1‑141 (MQ=255)
                                                                               cTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAg                                                          >  2:727863/1‑141 (MQ=255)
                                                                                  tGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCg                                                       >  2:826428/1‑141 (MQ=255)
                                                                                  gggCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCg                                                       <  2:553908/140‑1 (MQ=255)
                                                                                   ggCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGt                                                      >  1:523080/1‑141 (MQ=255)
                                                                                    gCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTa                                                     <  1:17805/141‑1 (MQ=255)
                                                                                     cTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCGAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaa                                                    >  2:936478/1‑141 (MQ=255)
                                                                                     cTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaa                                                    >  1:1050325/1‑141 (MQ=255)
                                                                                        tataCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAAAAAAAAAAAGGCGTaaaaa                                                 <  4:140599/141‑1 (MQ=255)
                                                                                        tataCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaaaaa                                                 <  1:56706/141‑1 (MQ=255)
                                                                                          taCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAAtt                                               >  4:72823/1‑141 (MQ=255)
                                                                                            atctAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAAACAAAAAAGGCGTAAAAATTGa                                             <  2:36008/140‑1 (MQ=255)
                                                                                            atctAAAAAGTATAAAAGATTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAAAAAAAAAAAGGCGTAAAAATTGa                                             <  2:766363/140‑1 (MQ=255)
                                                                                                 aaaaGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATg                                        >  1:796222/1‑141 (MQ=255)
                                                                                                    aGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTa                                     <  1:868670/141‑1 (MQ=255)
                                                                                                      tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCta                                                                                                                               <  1:57934/49‑1 (MQ=255)
                                                                                                      tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAg                                   >  3:197460/1‑141 (MQ=255)
                                                                                                      tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAg                                   >  3:136928/1‑141 (MQ=255)
                                                                                                        taAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGtt                                 >  1:54271/1‑141 (MQ=255)
                                                                                                        taAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGtt                                 >  2:845382/1‑141 (MQ=255)
                                                                                                         aaaaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGttt                                >  3:20391/1‑141 (MQ=255)
                                                                                                          aaaGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTa                               <  4:164807/141‑1 (MQ=255)
                                                                                                           aaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAg                              >  4:79915/1‑141 (MQ=255)
                                                                                                                 aaaaGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGatat                        >  2:1068040/1‑141 (MQ=255)
                                                                                                                   aaGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTa                      >  1:191442/1‑141 (MQ=255)
                                                                                                                    aGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTAc                     >  3:324198/1‑141 (MQ=255)
                                                                                                                       aTTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGACGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACaga                  >  1:801885/1‑141 (MQ=255)
                                                                                                                       aTTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACaga                  >  1:70056/1‑141 (MQ=255)
                                                                                                                        ttACCAAAAGGTGCAATAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                 <  4:403900/141‑1 (MQ=255)
                                                                                                                        ttACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                 >  3:143763/1‑141 (MQ=255)
                                                                                                                        ttACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTCAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                 <  2:1004628/141‑1 (MQ=255)
                                                                                                                          aCCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGaa               >  2:721949/1‑141 (MQ=255)
                                                                                                                           ccAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAAt              >  1:672694/1‑141 (MQ=255)
                                                                                                                             aaaaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaa            >  2:446832/1‑141 (MQ=255)
                                                                                                                              aaaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaa           >  2:636033/1‑141 (MQ=255)
                                                                                                                               aaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaa          >  1:252289/1‑141 (MQ=255)
                                                                                                                                aGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaaa         >  2:799079/1‑141 (MQ=255)
                                                                                                                                 ggTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaaaa        >  4:271498/1‑141 (MQ=255)
                                                                                                                                    gCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGat     >  1:768180/1‑141 (MQ=255)
                                                                                                                                    gCAACAGTCTATGTGTCGAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGat     <  2:713503/141‑1 (MQ=255)
                                                                                                                                      aaCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGatat   <  3:273210/141‑1 (MQ=255)
                                                                                                                                      aaCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGatat   <  2:718702/141‑1 (MQ=255)
                                                                                                                                       aCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATAtt  >  1:627908/1‑141 (MQ=255)
                                                                                                                                       aCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATAtt  <  1:19996/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                       
CAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATT  >  USA300TCH1516_ALE/895235‑895510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: