Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 895,408 T→C 6.1% R143R (CGT→CGC metQ_2 → Methionine‑binding lipoprotein MetQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE895,4080TC 6.1% 371.5 / 9.4 128R143R (CGT→CGCmetQ_2Methionine‑binding lipoprotein MetQ
Reads supporting (aligned to +/- strand):  ref base T (67/53);  new base C (4/4);  total (71/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 133 total aligned reads displayed.

GATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTACCTA  >  USA300TCH1516_ALE/895268‑895547
                                                                                                                                            |                                                                                                                                            
gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                                                              <  1:975331/141‑1 (MQ=255)
gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                                                              <  1:871248/141‑1 (MQ=255)
gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                                                              <  2:150477/141‑1 (MQ=255)
 aTAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGtt                                                                                                                                             >  1:692687/1‑141 (MQ=255)
  tAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGttt                                                                                                                                            <  4:402945/141‑1 (MQ=255)
     aGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTCTCtt                                                                                                                                         >  2:1022750/1‑141 (MQ=255)
        ttACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaa                                                                                                                                      >  1:156122/1‑141 (MQ=255)
         tACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaaa                                                                                                                                     >  4:6759/1‑141 (MQ=255)
         tACAAAATCGAAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCCAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaaa                                                                                                                                     <  4:409907/141‑1 (MQ=255)
          aCAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAt                                                                                                                                    <  1:348061/141‑1 (MQ=255)
           cAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAtt                                                                                                                                   <  3:262958/141‑1 (MQ=255)
              aaTCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAttctt                                                                                                                                >  4:250376/1‑141 (MQ=255)
                  gTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGtt                                                                                                                            >  1:523523/1‑141 (MQ=255)
                     aGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGAt                                                                                                                         >  4:10722/1‑141 (MQ=255)
                      gTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATg                                                                                                                        <  2:503296/141‑1 (MQ=255)
                      gTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATg                                                                                                                        <  4:328421/141‑1 (MQ=255)
                          cGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAgg                                                                                                                    <  3:269020/141‑1 (MQ=255)
                           ggTGCTGTTCAATTAGCACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGGATTTCCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGACCAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGt                                                                                                                   <  2:28181/141‑1 (MQ=255)
                            gTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGtt                                                                                                                  >  2:1041287/1‑141 (MQ=255)
                             tGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGttt                                                                                                                 <  1:390807/141‑1 (MQ=255)
                             tGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGttt                                                                                                                 <  3:110655/141‑1 (MQ=255)
                                tGTTGACGTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAGTCTTCGTTGATGCAGGTttaat                                                                                                              <  2:553772/141‑1 (MQ=255)
                                    cACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTaaa                                                                                                          <  3:301387/141‑1 (MQ=255)
                                    cACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTaaa                                                                                                          >  2:260025/1‑141 (MQ=255)
                                      cTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAAt                                                                                                        >  2:607189/1‑141 (MQ=255)
                                          gAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaa                                                                                                    >  3:454473/1‑141 (MQ=255)
                                            aCCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaaaa                                                                                                  >  2:376171/1‑141 (MQ=255)
                                            aCCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaaaa                                                                                                  >  3:253703/1‑141 (MQ=255)
                                              cTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAg                                                                                                >  2:727863/1‑141 (MQ=255)
                                                 tGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCg                                                                                             >  2:826428/1‑141 (MQ=255)
                                                  ggCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGt                                                                                            >  1:523080/1‑141 (MQ=255)
                                                   gCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTa                                                                                           >  2:1029629/1‑141 (MQ=255)
                                                   gCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTa                                                                                           <  1:17805/141‑1 (MQ=255)
                                                    cTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCGAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaa                                                                                          >  2:936478/1‑141 (MQ=255)
                                                    cTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaa                                                                                          >  1:1050325/1‑141 (MQ=255)
                                                       tataCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaaaaa                                                                                       <  1:56706/141‑1 (MQ=255)
                                                         taCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAAtt                                                                                     >  4:72823/1‑141 (MQ=255)
                                                           atctAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAAACAAAAAAGGCGTAAAAATTGa                                                                                   <  2:36008/140‑1 (MQ=255)
                                                           atctAAAAAGTATAAAAGATTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAAAAAAAAAAAGGCGTAAAAATTGa                                                                                   <  2:766363/140‑1 (MQ=255)
                                                              tAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGa                                                                                >  1:659909/1‑141 (MQ=255)
                                                              tAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCGTAAAATTCTTAGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAGGa                                                                                <  4:21271/141‑1 (MQ=255)
                                                                aaaaGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATg                                                                              >  1:796222/1‑141 (MQ=255)
                                                                  aaGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCt                                                                            <  4:299649/141‑1 (MQ=255)
                                                                   aGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTa                                                                           <  3:158464/141‑1 (MQ=255)
                                                                   aGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTa                                                                           <  1:868670/141‑1 (MQ=255)
                                                                     tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAg                                                                         >  3:136928/1‑141 (MQ=255)
                                                                     tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAg                                                                         >  3:197460/1‑141 (MQ=255)
                                                                       taAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGtt                                                                       >  2:845382/1‑141 (MQ=255)
                                                                        aaaaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGttt                                                                      >  3:20391/1‑141 (MQ=255)
                                                                         aaaGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTa                                                                     <  4:164807/141‑1 (MQ=255)
                                                                          aaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAg                                                                    >  4:79915/1‑141 (MQ=255)
                                                                                aaaaGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGatat                                                              >  2:1068040/1‑141 (MQ=255)
                                                                                  aaGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTa                                                            >  1:191442/1‑141 (MQ=255)
                                                                                   aGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTAc                                                           >  3:324198/1‑141 (MQ=255)
                                                                                     aaTTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACag                                                         <  1:337158/141‑1 (MQ=255)
                                                                                      aTTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGACGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACaga                                                        >  1:801885/1‑141 (MQ=255)
                                                                                      aTTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACaga                                                        >  1:70056/1‑141 (MQ=255)
                                                                                       ttACCAAAAGGTGCAATAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                                                       <  4:403900/141‑1 (MQ=255)
                                                                                       ttACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                                                       >  3:143763/1‑141 (MQ=255)
                                                                                       ttACCAAAAGGTGCAACAGTCTATGTGTCTAAGAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                                                       <  1:236569/141‑1 (MQ=255)
                                                                                         aCCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGaa                                                     >  2:721949/1‑141 (MQ=255)
                                                                                          ccAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAAt                                                    >  1:672694/1‑141 (MQ=255)
                                                                                            aaaaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaa                                                 >  2:446832/1‑141 (MQ=255)
                                                                                             aaaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaa                                                >  2:636033/1‑141 (MQ=255)
                                                                                              aaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaa                                               >  1:252289/1‑141 (MQ=255)
                                                                                               aGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaaa                                              >  2:799079/1‑141 (MQ=255)
                                                                                                ggTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaaaa                                             >  4:271498/1‑141 (MQ=255)
                                                                                                   gCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGat                                          >  1:768060/1‑141 (MQ=255)
                                                                                                   gCAACAGTCTATGTGTCGAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGat                                          <  2:713503/141‑1 (MQ=255)
                                                                                                     aaCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGatat                                        <  2:438386/141‑1 (MQ=255)
                                                                                                      aCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATAtt                                       >  1:627908/1‑141 (MQ=255)
                                                                                                      aCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATAtt                                       <  1:19996/141‑1 (MQ=255)
                                                                                                            tATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGttt                                 <  1:845382/141‑1 (MQ=255)
                                                                                                            tATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGttt                                 <  1:1022750/141‑1 (MQ=255)
                                                                                                              tgtgTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACGGAGAATAAAAAAGATATTAAGTTtaa                               >  4:348963/1‑141 (MQ=255)
                                                                                                              agtgtCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCTTTGATGCAGTTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTtaa                               <  2:123474/140‑1 (MQ=255)
                                                                                                                 gtCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTtaataa                            <  1:1069994/141‑1 (MQ=255)
                                                                                                                  tCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTtaataat                           >  2:254302/1‑141 (MQ=255)
                                                                                                                   cTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTtaataata                          <  1:988394/141‑1 (MQ=255)
                                                                                                                    taataaTCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTtaataataa                         <  1:503211/141‑1 (MQ=255)
                                                                                                                        aatCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACaa                     <  2:448352/141‑1 (MQ=255)
                                                                                                                          tCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATc                   >  2:477598/1‑141 (MQ=255)
                                                                                                                             aGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAAGATATTAAGTTTAATAATAAACAATcag                 >  1:622877/1‑141 (MQ=255)
                                                                                                                             aGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagc                <  3:132662/141‑1 (MQ=255)
                                                                                                                             aGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagc                >  1:565271/1‑141 (MQ=255)
                                                                                                                             aGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAGATAAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagc                <  2:266964/141‑1 (MQ=255)
                                                                                                                              gCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagca               >  3:441045/1‑141 (MQ=255)
                                                                                                                              gCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagca               >  1:278210/1‑141 (MQ=255)
                                                                                                                              gCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagca               >  1:894614/1‑141 (MQ=255)
                                                                                                                               cTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagcag              <  1:390344/141‑1 (MQ=255)
                                                                                                                               cTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATcagcag              <  1:574318/141‑1 (MQ=255)
                                                                                                                                   aCAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATAGTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAAtt          >  3:361741/1‑141 (MQ=255)
                                                                                                                                    cAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTc         >  3:190384/1‑141 (MQ=255)
                                                                                                                                      aGGACGTTTCTTAAAATTCTTCTTGGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCtt       >  4:177924/1‑141 (MQ=255)
                                                                                                                                      aGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCtt       >  4:184455/1‑141 (MQ=255)
                                                                                                                                         aCGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTAcc    <  1:662716/141‑1 (MQ=255)
                                                                                                                                          cGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTACCt   >  1:359544/1‑141 (MQ=255)
                                                                                                                                          cGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTACCt   <  1:434260/141‑1 (MQ=255)
                                                                                                                                          cGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTACCt   <  1:997936/141‑1 (MQ=255)
                                                                                                                                           gTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTACCTa  >  1:635992/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATATTAAGTTTAATAATAAACAATCAGCAGAATTCTTACCTA  >  USA300TCH1516_ALE/895268‑895547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: