Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 950,098 T→A 6.1% intergenic (+92/‑270) yugI_2 → / → USA300HOU_RS04740 General stress protein 13/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE950,0980TA 6.1% 429.2 / 13.1 132intergenic (+92/‑270)yugI_2/USA300HOU_RS04740General stress protein 13/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (67/56);  new base A (3/5);  total (71/61)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 143 total aligned reads displayed.

CTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGTTTTTT  >  USA300TCH1516_ALE/949958‑950238
                                                                                                                                            |                                                                                                                                              
 ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                               >  1:1012550/1‑141 (MQ=255)
 ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                               >  3:20115/1‑141 (MQ=255)
     cAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGAt                                                                                                                                           <  4:349218/141‑1 (MQ=255)
        tCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACt                                                                                                                                        <  2:290182/141‑1 (MQ=255)
          tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTaa                                                                                                                                      <  1:418807/141‑1 (MQ=255)
            gATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtc                                                                                                                                    >  3:88767/1‑141 (MQ=255)
             aTAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtct                                                                                                                                   <  1:327005/141‑1 (MQ=255)
              tAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtctc                                                                                                                                  >  2:219861/1‑141 (MQ=255)
               aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                                 <  1:122163/141‑1 (MQ=255)
                aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCtt                                                                                                                                <  2:433058/141‑1 (MQ=255)
                aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTGCTTATACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCtt                                                                                                                                <  2:533323/141‑1 (MQ=38)
                 aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                               >  4:139018/1‑141 (MQ=255)
                 aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                               <  2:34584/141‑1 (MQ=255)
                 aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                               >  3:86460/1‑141 (MQ=255)
                    attCAAAGCGAGCAATGCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTg                                                                                                                          <  2:124506/139‑1 (MQ=255)
                    aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTg                                                                                                                          <  1:712250/141‑1 (MQ=255)
                        aaaGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGACTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTa                                                                                                                      >  2:443723/1‑141 (MQ=255)
                          aGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAACATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACg                                                                                                                    >  3:376938/1‑141 (MQ=255)
                             gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATa                                                                                                                 >  1:287068/1‑141 (MQ=255)
                               gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAAc                                                                                                               >  3:404276/1‑141 (MQ=255)
                               gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAAc                                                                                                               >  1:303422/1‑141 (MQ=255)
                                 aaTTCGAAACGACTAAAGGACCAGATAAATCGTACCGAAAAACATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGt                                                                                                             <  1:597274/141‑1 (MQ=255)
                                 aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGt                                                                                                             <  1:91140/141‑1 (MQ=255)
                                 aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGt                                                                                                             >  2:430869/1‑141 (MQ=255)
                                   ttCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTaa                                                                                                           >  2:238286/1‑141 (MQ=255)
                                    tCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAat                                                                                                          >  2:621314/1‑141 (MQ=255)
                                     cGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAata                                                                                                         >  2:878168/1‑141 (MQ=255)
                                       aaaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATAtg                                                                                                       <  1:104037/141‑1 (MQ=255)
                                              tAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATgattgatt                                                                                                <  1:643000/141‑1 (MQ=255)
                                               aaaGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTc                                                                                               <  1:808900/141‑1 (MQ=255)
                                                  ggAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTAt                                                                                            >  1:712956/1‑141 (MQ=255)
                                                     aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGAATGATTCTATTTa                                                                                         >  4:367281/1‑141 (MQ=255)
                                                     aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTGATCTACGATAACGTAATATGATTGATTCTATTTa                                                                                         >  2:602685/1‑141 (MQ=255)
                                                      cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGGAATGAAAGTTTCTTAGGCTATAAAAGAGGTTAGTATTTATTAAATGTTAGTATATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                        >  3:42567/1‑141 (MQ=255)
                                                      cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                        <  3:249557/141‑1 (MQ=255)
                                                      cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                        <  1:206173/141‑1 (MQ=255)
                                                         aTAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACg                                                                                     >  1:10211/1‑141 (MQ=255)
                                                                 tACCGAAAATCATACATATTGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                             <  3:136246/141‑1 (MQ=255)
                                                                 tACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                             >  2:1030898/1‑141 (MQ=255)
                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTTTTTTAACGTAATATGATTGATTCTATTTACACTTACAAATgg                                                                            >  4:417071/1‑141 (MQ=255)
                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAACTgg                                                                            >  2:807678/1‑141 (MQ=255)
                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                            >  4:173339/1‑141 (MQ=255)
                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                            >  1:994858/1‑141 (MQ=255)
                                                                   ccGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGt                                                                           <  2:333389/141‑1 (MQ=255)
                                                                    cGAAAATCATACAAAGGGTGTGAAATGAAAGTTTCTTAGACTATAAAAGGGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt                                                                          <  2:935344/141‑1 (MQ=255)
                                                                    cGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt                                                                          <  3:144825/141‑1 (MQ=255)
                                                                       aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa                                                                       >  2:843095/1‑141 (MQ=255)
                                                                       aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTGTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa                                                                       >  3:18537/1‑141 (MQ=255)
                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                                 <  1:845946/79‑1 (MQ=255)
                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                                 >  2:845946/1‑79 (MQ=255)
                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                                 >  2:1054256/1‑141 (MQ=255)
                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                                 >  2:774569/1‑141 (MQ=255)
                                                                              aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACa                                                                >  2:63508/1‑141 (MQ=255)
                                                                              aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATCTACACGTACAAATGGTTTAAGGTGACa                                                                >  2:1011674/1‑141 (MQ=255)
                                                                               cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                               <  2:1041038/141‑1 (MQ=255)
                                                                               cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                               >  2:560165/1‑141 (MQ=255)
                                                                                  aGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATc                                                            <  2:261969/141‑1 (MQ=255)
                                                                                   gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                           <  1:519489/141‑1 (MQ=255)
                                                                                   gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                           >  1:389866/1‑141 (MQ=255)
                                                                                   gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                           <  2:484411/141‑1 (MQ=255)
                                                                                     gTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAt                                                         <  4:71101/141‑1 (MQ=255)
                                                                                      tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTGGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                        >  1:655431/1‑141 (MQ=255)
                                                                                      tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                        >  1:571585/1‑141 (MQ=255)
                                                                                       cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa                                                       >  1:249847/1‑141 (MQ=255)
                                                                                         gaaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATc                                                     >  2:958084/1‑141 (MQ=255)
                                                                                               aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                               <  1:624837/141‑1 (MQ=255)
                                                                                               aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                               <  1:634127/141‑1 (MQ=255)
                                                                                                  gTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTGTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtaga                                            <  1:427531/141‑1 (MQ=255)
                                                                                                   tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat                                           >  2:951778/1‑141 (MQ=255)
                                                                                                    ttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagata                                          >  2:90030/1‑141 (MQ=255)
                                                                                                        tAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAAt                                      >  3:356217/1‑141 (MQ=255)
                                                                                                         aGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATc                                     <  1:678644/141‑1 (MQ=255)
                                                                                                          gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGGCATATCCATTATCTTTGTTAGATAGAATCg                                    <  1:432801/141‑1 (MQ=255)
                                                                                                          gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCg                                    <  4:189670/141‑1 (MQ=255)
                                                                                                            cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATTCATTATCTTTGTTAGATAGAATCGtt                                  >  2:278320/1‑141 (MQ=255)
                                                                                                             acaaaaaGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCTTTTATAGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGt                                   >  1:81670/4‑141 (MQ=25)
                                                                                                              ataAAAGCGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTCAAACAGGTTTAACGTGATATCTCTATTCTCTTTGTTAGATAGAATCGTTGa                                <  4:42567/141‑1 (MQ=255)
                                                                                                                aaaaGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGAtt                              >  3:247641/1‑141 (MQ=255)
                                                                                                                 aaaGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGAttt                             <  3:334081/141‑1 (MQ=255)
                                                                                                                   agagATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGc                           <  2:217245/141‑1 (MQ=255)
                                                                                                                      gaTTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAat                        <  2:143668/141‑1 (MQ=255)
                                                                                                                        ttAGTATCTAATAAAATTTAATAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAatat                      >  2:490241/1‑141 (MQ=255)
                                                                                                                         tAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATAtt                     >  3:420955/1‑141 (MQ=255)
                                                                                                                          aGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg                    >  4:76049/1‑141 (MQ=255)
                                                                                                                          aGTATCTAATAAAATTTAATAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg                    >  1:289595/1‑141 (MQ=255)
                                                                                                                           gTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt                   >  1:192089/1‑141 (MQ=255)
                                                                                                                           gTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt                   <  4:297315/141‑1 (MQ=255)
                                                                                                                               cTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTAtgt               <  2:598500/141‑1 (MQ=255)
                                                                                                                                 aTTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTgg             <  1:33427/141‑1 (MQ=255)
                                                                                                                                  ttAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGa            <  2:684360/141‑1 (MQ=255)
                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTTTTTTTTTTTTTTTATAATATGATTGATTTTATTTATAAGTACAATTGGTTTAGGGTAACATATCTATTATTTTGGTTAGATAGAATCTTTGATTTTCATTATTGAATGTGTAtt          >  2:837673/1‑141 (MQ=255)
                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt          >  2:189212/1‑141 (MQ=255)
                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt          >  4:229379/1‑141 (MQ=255)
                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt          <  4:29342/141‑1 (MQ=255)
                                                                                                                                       ttttATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGt       >  1:661310/1‑141 (MQ=255)
                                                                                                                                         ttattaGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGttt     <  1:914583/141‑1 (MQ=255)
                                                                                                                                           attaGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGttttt   >  4:172518/1‑141 (MQ=255)
                                                                                                                                           attaGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGttttt   >  3:33354/1‑141 (MQ=255)
                                                                                                                                           attaGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGACAGAATCGTTGATTTGCAATATTGTATGTGGATTTgttgtt   >  1:15026/1‑141 (MQ=255)
                                                                                                                                            ttaGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGtttttt  <  4:197270/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTTTGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGTTTTTT  >  USA300TCH1516_ALE/949958‑950238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: