Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,034,893 G→A 5.2% H62H (CAC→CAT USA300TCH1516_00961 ← Lipoate‑‑protein ligase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,034,8930GA 5.2% 363.4 / 4.5 114H62H (CAC→CATUSA300TCH1516_00961Lipoate‑‑protein ligase 1
Reads supporting (aligned to +/- strand):  ref base G (51/57);  new base A (3/3);  total (54/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 124 total aligned reads displayed.

TTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCTAA  >  USA300TCH1516_ALE/1034755‑1035033
                                                                                                                                          |                                                                                                                                            
ttGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTtgtg                                                                                                                                            <  2:924159/141‑1 (MQ=255)
  gAACAATTGGTTCAGTGAATTTTTTTTTTTTTTTTAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAg                                                                                                                                          >  2:512987/1‑141 (MQ=255)
    aCAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCa                                                                                                                                        >  4:301007/1‑141 (MQ=255)
    aCAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCa                                                                                                                                        >  1:26919/1‑141 (MQ=255)
        ttGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGa                                                                                                                                    <  1:249869/141‑1 (MQ=255)
         tGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGat                                                                                                                                   >  2:626837/1‑141 (MQ=255)
            ttCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGatata                                                                                                                                <  4:76114/141‑1 (MQ=255)
                    aTTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGAt                                                                                                                        <  2:473540/141‑1 (MQ=255)
                     tttttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGAtt                                                                                                                       >  4:125254/1‑141 (MQ=255)
                     tttttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACAACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGAtt                                                                                                                       <  3:436399/141‑1 (MQ=255)
                       tttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTa                                                                                                                     >  1:464165/1‑141 (MQ=255)
                       tttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTa                                                                                                                     <  3:325503/141‑1 (MQ=255)
                         tGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACt                                                                                                                   <  1:999272/141‑1 (MQ=255)
                          ggAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACtt                                                                                                                  >  1:174581/1‑141 (MQ=255)
                          ggAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACtt                                                                                                                  >  1:529001/1‑141 (MQ=255)
                              aTTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCctc                                                                                                              <  4:322342/141‑1 (MQ=255)
                                    gAAACTGTTACCATCATCATCTGTTATACAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt                                                                                                        <  3:20225/141‑1 (MQ=255)
                                    gAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt                                                                                                        <  1:504160/141‑1 (MQ=255)
                                        cTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTAttt                                                                                                    <  1:474691/141‑1 (MQ=255)
                                         tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTAtttt                                                                                                   <  2:1030853/141‑1 (MQ=255)
                                         tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTAtttt                                                                                                   <  2:484132/141‑1 (MQ=255)
                                         tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTAtttt                                                                                                   <  3:128717/141‑1 (MQ=255)
                                           ttACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGa                                                                                                 >  1:123934/1‑141 (MQ=255)
                                             aCCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGAtt                                                                                               <  1:241773/141‑1 (MQ=255)
                                             aCCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGAtt                                                                                               >  2:967621/1‑141 (MQ=255)
                                              ccATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTc                                                                                              >  2:276325/1‑141 (MQ=255)
                                               catcatcgtCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTAATTCt                                                                                             >  1:857869/1‑141 (MQ=255)
                                               catcatcatCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCt                                                                                             <  2:956038/141‑1 (MQ=255)
                                               catcatcatCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCAACGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCt                                                                                             <  2:919746/141‑1 (MQ=255)
                                                       tcTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCcaaca                                                                                     <  1:1064342/141‑1 (MQ=255)
                                                         tGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAAt                                                                                   >  2:370898/1‑141 (MQ=255)
                                                          gTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATg                                                                                  >  1:596598/1‑141 (MQ=255)
                                                          gTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATg                                                                                  >  3:240211/1‑141 (MQ=255)
                                                               aaaaCTAAAGTTTACAGCTCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGa                                                                             <  1:603952/141‑1 (MQ=255)
                                                                aaaCTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGAt                                                                            <  3:429/141‑1 (MQ=255)
                                                                 aaCTANAGTTTAACTTGCCAGTATCCTGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGTATCGATATATGTCTGATTTACTTCCTCTNTCGTATTTTGATTCTTTCCAACAATGATAGATg                                                                           <  1:132987/141‑1 (MQ=255)
                                                                    tAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTc                                                                        <  2:104109/141‑1 (MQ=255)
                                                                    tAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTc                                                                        >  2:1065617/1‑141 (MQ=255)
                                                                     aaaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCt                                                                       >  2:330937/1‑141 (MQ=255)
                                                                      aaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTa                                                                      <  2:715775/141‑1 (MQ=255)
                                                                        gTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTGTCGTATTTTGATTCTTTCCAACAATGTTAGATGGTCTAtt                                                                    >  1:288911/1‑141 (MQ=255)
                                                                            aaaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATg                                                                <  3:190337/141‑1 (MQ=255)
                                                                             aaTTGCCAGTATCATGATAAACAGCGCCACTACCAGAAATTCTTCTTACTACTTCTATGTTGTGAGCATCGATATATGTCTGATTTACTTTCTCTATCGTATTTTGATTCTTTCCAACTATGATAGATAGTCTATTTATGt                                                               <  4:274331/141‑1 (MQ=255)
                                                                             aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt                                                               >  3:323754/1‑141 (MQ=255)
                                                                              aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATTGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta                                                              <  2:197666/141‑1 (MQ=255)
                                                                              aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta                                                              <  1:141189/141‑1 (MQ=255)
                                                                              aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta                                                              >  2:49933/1‑141 (MQ=255)
                                                                                 gCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATGTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaa                                                           <  4:31810/141‑1 (MQ=255)
                                                                                  ccAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaat                                                          <  1:265238/141‑1 (MQ=255)
                                                                                       aTCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATaaaaa                                                     <  2:893459/141‑1 (MQ=255)
                                                                                        tCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAg                                                    >  2:374566/1‑141 (MQ=255)
                                                                                         cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt                                                   <  4:408751/141‑1 (MQ=255)
                                                                                         cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt                                                   <  4:26581/141‑1 (MQ=255)
                                                                                          aTGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTa                                                  <  1:691814/141‑1 (MQ=255)
                                                                                            gATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAAc                                                >  2:340035/1‑141 (MQ=255)
                                                                                            gATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAAc                                                >  1:669101/1‑141 (MQ=255)
                                                                                             aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt                                               <  1:337950/141‑1 (MQ=255)
                                                                                             aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGATGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt                                               <  3:64665/141‑1 (MQ=255)
                                                                                               aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                                             <  4:438448/141‑1 (MQ=255)
                                                                                               aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                                             >  4:80803/1‑141 (MQ=255)
                                                                                               aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                                             >  2:614821/1‑141 (MQ=255)
                                                                                               aaaCAGCGCCACCAACAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                                             <  4:438446/141‑1 (MQ=255)
                                                                                                   aGCGCCACCACCAGAAATTCTTCTTACTACATCGAGGTCGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGAATATGTATGTAAAATAAAAAGTAANGtccttc                                         >  2:813005/1‑141 (MQ=255)
                                                                                                    gcgcCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTtcttct                                        >  3:61616/1‑141 (MQ=255)
                                                                                                     cgcCACCACCAGACATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTctg                                       >  2:98404/1‑141 (MQ=255)
                                                                                                       ccaccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTctgct                                     <  1:255808/141‑1 (MQ=255)
                                                                                                        caccaccaGAAATGCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATACAAAGTAACTTTCTTctgctg                                    >  4:455876/1‑141 (MQ=255)
                                                                                                        caccaccaGAAATGCTTCTAACTACATCGATGTTATGAACATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAGAAAGTAACTTTCTTctgctt                                    >  4:455982/1‑140 (MQ=255)
                                                                                                         accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg                                   <  2:605801/141‑1 (MQ=255)
                                                                                                         accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg                                   <  1:486156/141‑1 (MQ=255)
                                                                                                         accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg                                   <  3:231155/141‑1 (MQ=255)
                                                                                                          ccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGt                                  >  1:231895/1‑141 (MQ=255)
                                                                                                             ccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTAATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTaaa                               <  2:786267/141‑1 (MQ=255)
                                                                                                              caGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAt                              >  3:87225/1‑141 (MQ=255)
                                                                                                               aGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtt                             >  1:913/1‑141 (MQ=255)
                                                                                                                gAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAttt                            <  1:729597/141‑1 (MQ=255)
                                                                                                                 aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt                           <  2:596598/141‑1 (MQ=255)
                                                                                                                 aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt                           >  4:133966/1‑141 (MQ=255)
                                                                                                                 aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt                           >  2:919607/1‑141 (MQ=255)
                                                                                                                 aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt                           >  2:284171/1‑141 (MQ=255)
                                                                                                                  aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAttttt                          <  2:122772/141‑1 (MQ=255)
                                                                                                                  aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTAGTAAAttttt                          <  3:202757/141‑1 (MQ=255)
                                                                                                                    ttcttcTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTa                        >  1:834695/1‑141 (MQ=255)
                                                                                                                     tcttctTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaa                       >  3:191896/1‑141 (MQ=255)
                                                                                                                      cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa                      >  1:777575/1‑141 (MQ=255)
                                                                                                                      cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa                      >  4:221275/1‑141 (MQ=255)
                                                                                                                      cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca                                                                                        <  4:104032/75‑1 (MQ=255)
                                                                                                                      cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca                                                                                        >  3:104032/1‑75 (MQ=255)
                                                                                                                          ttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACat                  <  1:400382/141‑1 (MQ=255)
                                                                                                                          ttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACat                  <  1:994309/141‑1 (MQ=255)
                                                                                                                           tactacATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACata                 <  2:698520/141‑1 (MQ=255)
                                                                                                                                 aTCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATAttcttc           <  1:756570/141‑1 (MQ=255)
                                                                                                                                    gATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCAt        >  3:194190/1‑141 (MQ=255)
                                                                                                                                     aTGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCAtt       <  3:64679/141‑1 (MQ=255)
                                                                                                                                      tgttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTg      >  2:166706/1‑141 (MQ=255)
                                                                                                                                       gttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGc     >  1:731933/1‑141 (MQ=255)
                                                                                                                                       gttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGc     <  2:677596/141‑1 (MQ=255)
                                                                                                                                       gttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGc     >  1:440958/1‑141 (MQ=255)
                                                                                                                                       gttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGc     <  1:67694/141‑1 (MQ=255)
                                                                                                                                        ttgtGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCt    <  3:417770/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATATTCTTCCATTGCTAA  >  USA300TCH1516_ALE/1034755‑1035033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: