BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,051,886	T→G	7.4%	R101R (CGA→CGC)	sspC ←	Staphostatin B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,051,886	0	T	G	7.4%	318.0 / 15.8	118	R101R (CGA→CGC)	sspC	Staphostatin B
Reads supporting (aligned to +/- strand): ref base T (49/59); new base G (5/4); total (55/63)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 118 total aligned reads displayed.

AATTAATAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATAAAAAGAA  >  USA300TCH1516_ALE/1051747‑1052025

aaTTAATAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTc                                                                                                                                            >  1:691168/1‑141 (MQ=255)

aaTTAATAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTc                                                                                                                                            <  1:924895/141‑1 (MQ=255)

aaTTAATAGAGAAAGAATAAATGTTAAAGATTATAAATAAAAAGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTc                                                                                                                                            <  2:19163/141‑1 (MQ=255)

    aaTAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGa                                                                                                                                        >  2:265095/1‑141 (MQ=255)

     aTAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGCGaa                                                                                                                                       >  2:146162/1‑141 (MQ=255)

      tAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAg                                                                                                                                      >  4:364731/1‑141 (MQ=255)

      tAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAg                                                                                                                                      >  2:392507/1‑141 (MQ=255)

         agaaagaaTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGc                                                                                                                                   <  2:411701/141‑1 (MQ=255)

            aagaaTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCaa                                                                                                                                >  3:181926/1‑141 (MQ=255)

             agaaTAAATGTTAGAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAAt                                                                                                                               <  4:380872/141‑1 (MQ=255)

             agaaTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCaaa                                                                                                                               >  2:718459/1‑140 (MQ=255)

             agaaTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAAt                                                                                                                               >  2:458445/1‑141 (MQ=255)

              gaaTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATa                                                                                                                              <  2:981305/141‑1 (MQ=255)

                aTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGGAATCCCATCCCTTCGTTATACTAAGCACTCAAAAAAGATTGGAAGCGAAGTGCCAATAcc                                                                                                                            >  1:491269/1‑141 (MQ=255)

                aTAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTGAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATAcc                                                                                                                            >  4:130892/1‑141 (MQ=255)

                aTAAATGTAAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATAcc                                                                                                                            <  4:186773/141‑1 (MQ=255)

                    aTGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGt                                                                                                                        >  1:275933/1‑141 (MQ=255)

                    aTGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGt                                                                                                                        >  2:196744/1‑141 (MQ=255)

                      tttAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGttt                                                                                                                      <  3:425844/140‑1 (MQ=255)

                        tAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTgg                                                                                                                    >  2:336752/1‑141 (MQ=255)

                        tAAAGATAATTATTACAATGAAACAACGAGCTGGTGAGAAATCGACTTCTAGCCAACCCGCTTTATGAAGTGAAAGCATTTAAATAAGTTACACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTgc                                                                                                                    <  4:109709/141‑2 (MQ=255)

                          aaGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGAt                                                                                                                  >  2:971626/1‑141 (MQ=255)

                          aaGATTATTATTACAATGAAAAAACGAGCTGGTGAAAAATTGAATTATAGCAAACCAGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGACAATAAATAGTTTGGaa                                                                                                                  >  4:384867/1‑140 (MQ=255)

                           aGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATg                                                                                                                 <  1:261229/141‑1 (MQ=255)

                              ttattattaCAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACg                                                                                                              <  3:147518/141‑1 (MQ=255)

                              ttattattaCAATGAAAAAACGAGCTCGTGAGAAATTGAATGCTCGCCAACTCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGTTCGCGAAGTGCCAATACCTTGTTTGGATGACg                                                                                                              <  2:946924/141‑1 (MQ=255)

                                attattaCAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGtt                                                                                                            <  1:812865/141‑1 (MQ=255)

                                  tattaCAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTg                                                                                                          <  2:507323/141‑1 (MQ=255)

                                  tattaCAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTg                                                                                                          <  1:75386/141‑1 (MQ=255)

                                      aCAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATg                                                                                                      >  1:1025908/1‑141 (MQ=255)

                                        aaTGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAg                                                                                                    <  2:157588/141‑1 (MQ=255)

                                           gAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGat                                                                                                 <  2:305077/141‑1 (MQ=255)

                                                aaCGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCt                                                                                            <  1:982701/141‑1 (MQ=255)

                                                 aCGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCtg                                                                                           <  2:74540/141‑1 (MQ=255)

                                                     gCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTa                                                                                       >  2:366861/1‑141 (MQ=255)

                                                      cTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTat                                                                                      >  1:86374/1‑141 (MQ=255)

                                                      cTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATGGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTat                                                                                      >  1:86390/1‑141 (MQ=255)

                                                       tGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatc                                                                                     <  1:528290/141‑1 (MQ=255)

                                                         gggggaAATGGATATCACGACAACCCGCTTTATGAAGTGAAATCGTTTAAATTAGTTAGACTAAGCGTTCATAACCGATTGGTCGGGAAGTGCCAGTACCTTGTTTGGATGACGTTTGCATGAGGATGTGCTGGGTatcat                                                                                   <  4:368441/137‑1 (MQ=255)

                                                         gTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAGTTAGTTATACTAGGCGCTCATAAACGATTGGGCGCGAAGTGCCAATACCGTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatcat                                                                                   >  1:678403/1‑141 (MQ=255)

                                                         gTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatcat                                                                                   >  4:363471/1‑141 (MQ=255)

                                                         gTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatcat                                                                                   >  2:765883/1‑141 (MQ=255)

                                                         gTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatcat                                                                                   <  2:70073/141‑1 (MQ=255)

                                                         gTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatcat                                                                                   <  2:67329/141‑1 (MQ=255)

                                                          tGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTatcatc                                                                                  <  1:246109/141‑1 (MQ=255)

                                                           gagaAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTAtcatca                                                                                 <  1:990227/141‑1 (MQ=255)

                                                           gagaAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTAtcatca                                                                                 >  4:273529/1‑141 (MQ=255)

                                                            agaAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATcatcat                                                                                <  2:61557/141‑1 (MQ=255)

                                                              aaaTTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATaa                                                                              >  2:592287/1‑141 (MQ=255)

                                                                 ttGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCa                                                                           <  1:542676/141‑1 (MQ=255)

                                                                    aaTTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGa                                                                        >  2:1044417/1‑141 (MQ=255)

                                                                         tcGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAAtata                                                                   >  1:743850/1‑141 (MQ=255)

                                                                           gcaaccccGCTTTATGAACTGAAATCACTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGAGAAGTGCAACTACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATaag                                                                 <  2:215179/136‑2 (MQ=255)

                                                                           gCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATaaa                                                                 <  1:109925/141‑1 (MQ=255)

                                                                           gCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATaag                                                                 <  4:260930/141‑2 (MQ=255)

                                                                             cAACCCGCTTTATGAAGTGAAATAACGTAAATTAGTCATACTAAGCGCTCATAAACGATTGTTCGCGAAGTGCCAATACCTTGTTTGTAGGAAGTTTGCATGAGGAAATGCTGTGTATCATCATAAGCAAGAATATAAATg                                                               >  4:212778/1‑141 (MQ=255)

                                                                               aCCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGAt                                                             >  2:668857/1‑141 (MQ=255)

                                                                                 ccGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGAttt                                                           <  1:740220/141‑1 (MQ=255)

                                                                                 ccGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGAttt                                                           <  2:926210/141‑1 (MQ=255)

                                                                                 ccGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGAttt                                                           <  3:14265/141‑1 (MQ=255)

                                                                                  cGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATttc                                                          >  3:16899/1‑141 (MQ=255)

                                                                                  cGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAGTGATttc                                                          <  3:35636/141‑1 (MQ=255)

                                                                                   gCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATAACATTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATttct                                                         <  1:522363/141‑1 (MQ=255)

                                                                                        aTGAAGTGAAATCATTCAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCAtt                                                    <  3:251167/141‑1 (MQ=255)

                                                                                         tGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTa                                                   >  2:141636/1‑141 (MQ=255)

                                                                                             gTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCaa                                               >  1:365416/1‑141 (MQ=255)

                                                                                              tGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGGCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAGTGATTTCTTCATTATCAAt                                              >  1:546799/1‑141 (MQ=255)

                                                                                                 aaTCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGa                                           <  2:393864/141‑1 (MQ=255)

                                                                                                   tCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATg                                         <  1:354641/141‑1 (MQ=255)

                                                                                                    cATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGa                                        >  3:369591/1‑141 (MQ=255)

                                                                                                    cATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGa                                        <  2:673165/141‑1 (MQ=255)

                                                                                                      tttAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAAt                                                                             >  1:16577/1‑102 (MQ=255)

                                                                                                      tttAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGaaa                                      <  3:327965/141‑1 (MQ=255)

                                                                                                      tttAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGaaa                                      >  2:254729/1‑141 (MQ=255)

                                                                                                        tAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAAt                                    <  2:16668/141‑1 (MQ=255)

                                                                                                         aaaTTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAAtt                                   >  1:549092/1‑141 (MQ=255)

                                                                                                          aaTTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAAttt                                  >  1:31527/1‑141 (MQ=255)

                                                                                                           aTTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTa                                 >  4:240046/1‑141 (MQ=255)

                                                                                                            ttAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTaa                                >  4:173860/1‑141 (MQ=255)

                                                                                                            ttAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTaa                                <  3:421467/141‑1 (MQ=255)

                                                                                                              aGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGATGTTTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATg                              >  1:293919/1‑141 (MQ=255)

                                                                                                               gTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGc                             >  2:189003/1‑141 (MQ=255)

                                                                                                                 tataCTAAGCGCTCATAAATGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGt                           <  1:439028/141‑1 (MQ=255)

                                                                                                                    aCTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGa                        <  3:126803/141‑1 (MQ=255)

                                                                                                                    aCTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAACACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCAGCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGa                        <  1:582515/141‑1 (MQ=255)

                                                                                                                     cTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGAt                       >  1:42265/1‑141 (MQ=255)

                                                                                                                           gcTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCg                 >  4:121436/1‑141 (MQ=255)

                                                                                                                             tCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGGTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTa               <  2:663994/141‑1 (MQ=255)

                                                                                                                                  aaCGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCtat          <  2:498915/141‑1 (MQ=255)

                                                                                                                                   aCGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCtata         <  1:458445/141‑1 (MQ=255)

                                                                                                                                     gATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATaaa       <  1:141636/141‑1 (MQ=255)

                                                                                                                                     gATTGGGCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAGTGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATaaa       <  3:347211/141‑1 (MQ=255)

                                                                                                                                      aTTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATaaaa      >  1:331298/1‑141 (MQ=255)

                                                                                                                                      aTTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATaaaa      <  2:1031323/141‑1 (MQ=255)

                                                                                                                                       ttGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATaaaaa     >  3:174017/1‑141 (MQ=255)

                                                                                                                                        tGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATAAAAAg    >  2:763345/1‑141 (MQ=255)

                                                                                                                                         ggTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTGTCTATAAAAAGa   <  3:364731/141‑1 (MQ=255)

                                                                                                                                         ggTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCAGTATCTATAAAAAGa   <  2:222389/141‑1 (MQ=255)

                                                                                                                                          gTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATAAAAAGaa  >  1:470347/1‑141 (MQ=255)

                                                                                                                                          gTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAAATTAATGCGTTGATGTGCCGTATCTATAAAAAGaa  >  2:938861/1‑141 (MQ=255)

AATTAATAGAGAAAGAATAAATGTTAAAGATTATTATTACAATGAAAAAACGAGCTGGTGAGAAATTGAATTCTCGCCAACCCGCTTTATGAAGTGAAATCATTTAAATTAGTTATACTAAGCGCTCATAAACGATTGGTCGCGAAGTGCCAATACCTTGTTTGGATGACGTTTGCATGAGGATATGCTGTGTATCATCATAATCAAGAATATAAATGATTTCTTCATTATCAATAGATGAAAATTTAATGCGTTGATGTGCCGTATCTATAAAAAGAA  >  USA300TCH1516_ALE/1051747‑1052025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`