| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,252,446 | A→G | 13.4% | K207K (AAA→AAG) | ffh → | Signal recognition particle protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,252,446 | 0 | A | G | 13.4% | 65.9 / 3.6 | 30 | K207K (AAA→AAG) | ffh | Signal recognition particle protein |
| Reads supporting (aligned to +/- strand): ref base A (11/15); new base G (2/2); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
GTGAAGGAGATCAAGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGATCAACTTGATGTCACAGGTGTTACCTTAACTAAA > USA300TCH1516_ALE/1252310‑1252575 | gTGAAGTAGAGAAAGTAAAGGCACAACAAATTGTAACTAATGCATTAAAACGTGCTAAAGAAGAACATTTAAACTTTATAATCATGTATACAGCAGGTTGATTACACATCGATGAAGCATTGATGAACGAATTAAaagaag < 2:187984/141‑1 (MQ=255) aggatctCAAGTAAACCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCATGTCGATTACACATCTATGAATCATTGATTAACGAATTAAAAGAAGTaaa < 1:115993/135‑1 (MQ=255) aGGAGATCAAGTAAAGCCACAACAAATTGTGACGATTCAAGCAGAAGATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTaaa < 1:175602/141‑1 (MQ=255) aGGAGATCAAGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTaaa < 1:312061/141‑1 (MQ=255) gagaTCAAGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAg > 1:155228/1‑141 (MQ=255) gaTCAAGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGAc < 1:164216/141‑1 (MQ=255) cAAGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACAtt > 2:38365/1‑141 (MQ=255) aGTAAAGCTACAGCAAAGTGTAACTAATGCATTAGAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGc < 2:155177/141‑1 (MQ=255) aGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGc > 2:13113/1‑141 (MQ=255) gTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTCCAGTCAAAGATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCt < 1:134117/141‑1 (MQ=255) gTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAGGAAGTAAAAGACATTGCt > 1:166048/1‑141 (MQ=255) gTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAGGAAGTAAAAGACATTGCt > 1:389158/1‑141 (MQ=255) gTAAAGACACAACAAATTGTTTCAATCTCATTAAAACATGCTAAAGAACAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCt > 2:403054/1‑141 (MQ=255) aGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAAc > 1:331859/1‑141 (MQ=255) accacaaTTTGAAACTAATGCAAGAAAACATGCGAAAGAAGAACATGTAGACTTTGTAATCATCGATACAGCAGGGCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCaaa < 1:127546/138‑1 (MQ=255) aaTTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAAt > 1:79108/1‑141 (MQ=255) tGTAACTAATGCATTAAAACATGCTAAATAAAAACAGTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATAAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTAt > 2:90432/1‑141 (MQ=255) tGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGt < 1:30678/141‑1 (MQ=255) tAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGAtt < 2:221356/141‑1 (MQ=255) tAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGTTGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAGTTCTGTTAGATGTCGATTCATTGAAggg > 2:9323/1‑141 (MQ=255) agaagaACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGGATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGtaa > 1:368538/1‑139 (MQ=255) cATTTAGACTTTGTAATGATTGATACAGAAGGTCGATTACACATAGAAGAAGCATTGATGAAAGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCt < 2:197618/141‑1 (MQ=255) ttaTTGATACAGTAGGTTGATTACACATAGATGAAGCATTGATGAACGAATTAAAAGTAGTAAAAGACATTGCTAAACCAAACGAATTTATGTTAGTTGTTGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAAt < 2:300083/139‑1 (MQ=255) gATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCtttt > 1:399290/1‑141 (MQ=255) aCAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGAc > 1:352500/1‑141 (MQ=255) agcagGCCAATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACAATGCTAAACCAAACGAAATTTTGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGa < 1:236996/141‑1 (MQ=255) ttACACATCGATGAAGCATTGATGAACGAATTAAAGGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGATCAACTTGAt < 2:166048/141‑1 (MQ=255) atACACATCGATGAAGCATTGATGAACGAATTAAAGGAAGAAAAAGAAATTGCTAAACCAAGCGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGACTCTGTCAATGGTGCAGAATCTTTTGACGATCAACTTGAt < 2:389158/140‑1 (MQ=255) caTGGAGGAAGGATTGATGATGGAATTAAAAGAAGTAAAAGACATTGCTAAACAACACGAAATTATGTTAGTTGTCGAGTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGATCAACTTGATGTcac < 2:122894/141‑1 (MQ=255) cATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGATCAACTTGATGTCACAGGTGTTACCt < 1:381168/141‑1 (MQ=255) aaCGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGATCAACTTGATGTCACAGGTGTTACCTTAACTaaa < 1:163398/141‑1 (MQ=255) | GTGAAGGAGATCAAGTAAAGCCACAACAAATTGTAACTAATGCATTAAAACATGCTAAAGAAGAACATTTAGACTTTGTAATCATTGATACAGCAGGTCGATTACACATCGATGAAGCATTGATGAACGAATTAAAAGAAGTAAAAGACATTGCTAAACCAAACGAAATTATGTTAGTTGTCGATTCAATGACGGGTCAAGATGCTGTCAATGTTGCAGAATCTTTTGACGATCAACTTGATGTCACAGGTGTTACCTTAACTAAA > USA300TCH1516_ALE/1252310‑1252575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |