Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,416,458 A→T 100% I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,416,4580AT100.0% 83.8 / NA 29I216N (ATT→AAT) oppD_4Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/16);  total (13/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTG  >  USA300TCH1516_ALE/1416323‑1416590
                                                                                                                                       |                                                                                                                                    
ttAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTAc                                                                                                                                 >  2:148905/1‑141 (MQ=255)
      cAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACaa                                                                                                                           <  2:116932/141‑1 (MQ=255)
       aaTCATAGTAATCCCTCTTTAATTGTGTTATATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAat                                                                                                                          <  2:140262/141‑1 (MQ=255)
            tAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                     <  2:395604/141‑1 (MQ=255)
                 aTCCGTCTGTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATAc                                                                                                                <  2:385129/141‑1 (MQ=255)
                              tgtgTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTa                                                                                                   >  2:214843/1‑141 (MQ=255)
                                             aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTATCTCGTTCAATGATTTGAACTTATTTTAAAACATAATTGTACTGACAATATTTCATTTCATGACTTAGATTATGTATGTTactaaac                                                                                    >  2:278647/1‑140 (MQ=255)
                                                 gACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTGCTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                                                                <  2:223281/141‑1 (MQ=255)
                                                  aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATTATTCGCCCATATCTGGGGACGTAATTGTACTGACATGATGTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                               <  1:195481/141‑1 (MQ=255)
                                                     ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                            <  1:177620/141‑1 (MQ=255)
                                                       cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                          <  2:199335/141‑1 (MQ=255)
                                                       cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATATGTGATAATAAATAATTTTTGAc                                                                          >  1:356081/1‑141 (MQ=255)
                                                        aGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTTTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                         <  1:260977/141‑1 (MQ=255)
                                                                    cAAATGCTCATAATTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                             <  1:112192/141‑1 (MQ=255)
                                                                       aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTCTTAAAACATAATTGAAATCACAATATTTCAATACATGACTTAAGTACTGTGTGATAATAAATAATGATAGACCACGTTCTAATACaaa                                                          >  2:124390/1‑140 (MQ=255)
                                                                                           tAATATTACCTCGTTCAATTATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                      <  1:35004/141‑1 (MQ=255)
                                                                                                    cTCGTTCAATGATTTGTCAATAAAATAAGACAGAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGATTGACCATGTTCTAATACAATATGCTATAATAAATCCATCACTTAATTAc                             >  2:230732/1‑141 (MQ=255)
                                                                                                    cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAc                             >  1:91349/1‑141 (MQ=255)
                                                                                                     tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAGTAAATCCATCACTTGATTAcc                            >  1:120958/1‑141 (MQ=255)
                                                                                                     tCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATAACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTACTACAATATGCTGTAATAAATCCATCACTTGATTAcc                            >  2:231873/1‑141 (MQ=255)
                                                                                                               aTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCa                  <  1:332393/141‑1 (MQ=255)
                                                                                                               aTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAAGAAATCCATCACTTGATTACCGTTCAAAgta                  <  2:405005/141‑3 (MQ=255)
                                                                                                               aTTTGACCTTCTTTTAAAACATAATTGTACTGAAAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCa                  <  2:353661/141‑1 (MQ=255)
                                                                                                               aTTTGACATTCTTTTAAAACATAATTGTAATGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCa                  <  1:108644/141‑1 (MQ=255)
                                                                                                                  tGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATcc               <  1:148905/141‑1 (MQ=255)
                                                                                                                    aCCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa             >  1:234750/1‑141 (MQ=255)
                                                                                                                             tAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAAc    >  2:115019/1‑141 (MQ=255)
                                                                                                                               aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATGAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTg  >  1:219933/1‑141 (MQ=255)
                                                                                                                               aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACGGATGAAAGCATCCAATGATGCAACTg  >  1:257600/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                    
TTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTG  >  USA300TCH1516_ALE/1416323‑1416590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: