Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	9,280	G→A	R750H (CGT→CAT)	gyrA →	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	9,280	0	G	A	100.0%	313.0 / NA	85	R750H (CGT→CAT)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/34);  total (51/34)

GTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCGTAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCGCT  >  USA300TCH1516_ALE/9147‑9414                                                                                                                                      |                                                                                                                                       gTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggt                                                                                                                                 <  1:524097/141‑1 (MQ=255)    gCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggtaat                                                                                                                              >  4:211352/1‑141 (MQ=255)     cTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGACAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggtaatg                                                                                                                             >  2:762482/1‑141 (MQ=255)     cTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggtaatg                                                                                                                             >  4:59649/1‑141 (MQ=255)      tcctGCAAACAGTGTTGATGAAGTATCACTCGTTACAGTGAATGGTTATTGCAAACGTACGCCAGTGAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAAtgt                                                                                                                            <  1:745884/138‑1 (MQ=255)          gCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgta                                                                                                                        >  2:692268/1‑141 (MQ=255)           cAAACAGTGTTGATGAAGTATTAGTAGTTACTGATAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGGGGTGGTAAAGGTATTAAAACAGCTACGCTTACTGAGCATAATGGTAATGTtgtat                                                                                                                       <  2:645059/141‑1 (MQ=255)           cAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtat                                                                                                                       <  4:177863/141‑1 (MQ=255)           cAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtat                                                                                                                       <  3:296044/141‑1 (MQ=255)           cAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtat                                                                                                                       >  1:330005/1‑141 (MQ=255)            aaaCAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtatg                                                                                                                      >  2:53645/1‑141 (MQ=255)                 gtgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCa                                                                                                                 >  3:242292/1‑141 (MQ=255)                 gtgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATCTTGTATGTATCa                                                                                                                 >  4:204762/1‑141 (MQ=255)                  tgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCAc                                                                                                                >  1:298593/1‑141 (MQ=255)                  tgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCAc                                                                                                                <  1:207651/141‑1 (MQ=255)                     tgatgaAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTAc                                                                                                             >  1:224044/1‑141 (MQ=255)                     tgaagaAGTATTAGTATTTACTGAAAAAGGTTATGGAAAAAGTACACCAGTTAATGACTATCGAATAAAAAATCGAGGTGCGAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTCGATGTATCACTAc                                                                                                             >  4:220448/1‑141 (MQ=255)                         gaAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTa                                                                                                         >  3:263249/1‑141 (MQ=255)                         gaAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTa                                                                                                         <  1:185626/141‑1 (MQ=255)                                tagtagTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTg                                                                                                  <  2:414073/141‑1 (MQ=255)                                tagtagTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTg                                                                                                  >  1:93602/1‑141 (MQ=255)                                 agtagtTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTga                                                                                                 >  2:290263/1‑141 (MQ=255)                                    agtTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTgaaga                                                                                              >  3:122577/1‑141 (MQ=255)                                     gtTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGAgaagaa                                                                                             >  2:229691/1‑141 (MQ=255)                                         cTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGAtt                                                                                         >  4:33582/1‑141 (MQ=255)                                           gAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggta                                                                                                                                >  2:208464/1‑100 (MQ=255)                                           gAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggta                                                                                                                                <  1:208464/100‑1 (MQ=255)                                             aaaTGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAAt                                                                                     <  2:124120/141‑1 (MQ=255)                                               aTGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGa                                                                                   <  3:3834/141‑1 (MQ=255)                                               aTGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGa                                                                                   >  3:192219/1‑141 (MQ=255)                                                 ggTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGAtt                                                                                 >  2:490528/1‑141 (MQ=255)                                                  gTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTg                                                                                <  2:298593/141‑1 (MQ=255)                                                  attATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTGATGATTg                                                                                <  1:628931/140‑1 (MQ=255)                                                   ttATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGt                                                                               >  4:75907/1‑141 (MQ=255)                                                   ttATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGt                                                                               >  2:19569/1‑141 (MQ=255)                                                   ttATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGt                                                                               >  3:150814/1‑141 (MQ=255)                                                            aCGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGc                                                                      <  4:111518/141‑1 (MQ=255)                                                              gTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAg                                                                    >  3:283884/1‑141 (MQ=255)                                                                aCGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgt                                                                  >  2:314505/1‑141 (MQ=255)                                                                 cGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgtg                                                                 >  1:107269/1‑141 (MQ=255)                                                                 cGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgtg                                                                 <  1:457788/141‑1 (MQ=255)                                                                  gCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgtgt                                                                >  3:148644/1‑141 (MQ=255)                                                                   ccAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTc                                                               >  4:232673/1‑141 (MQ=255)                                                                     aGTTACTGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTACACCAGATCCGATAACCGAGCACATAGGAAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCat                                                             <  3:207788/141‑1 (MQ=255)                                                                     aGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCCGGTGTCat                                                             <  2:163851/141‑1 (MQ=255)                                                                      gTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCatt                                                            >  1:45275/1‑141 (MQ=255)                                                                        tAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCattat                                                          <  1:22814/141‑1 (MQ=255)                                                                        tAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTAGCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCattat                                                          <  1:77252/141‑1 (MQ=255)                                                                           tGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCg                                                       >  1:328399/1‑141 (MQ=255)                                                                              cTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACt                                                    <  1:229691/141‑1 (MQ=255)                                                                               ttatgCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTa                                                   <  1:339644/137‑1 (MQ=255)                                                                                   gCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATg                                               >  2:36029/1‑141 (MQ=255)                                                                                      tATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTg                                            >  3:288956/1‑141 (MQ=255)                                                                                        tCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTaa                                                                                      <  1:429617/97‑1 (MQ=255)                                                                                        tCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTaa                                                                                      >  2:429617/1‑97 (MQ=255)                                                                                          aaaTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGa                                        <  1:315471/141‑1 (MQ=255)                                                                                          aaaTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGa                                        <  4:139162/141‑1 (MQ=255)                                                                                           aaTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGat                                       >  1:760302/1‑141 (MQ=255)                                                                                            aTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGata                                      <  4:197441/141‑1 (MQ=255)                                                                                             tCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGatat                                     >  1:227830/1‑141 (MQ=255)                                                                                             tCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGatat                                     <  3:33582/141‑1 (MQ=255)                                                                                               gtggtgGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATAttt                                   >  1:233832/1‑141 (MQ=255)                                                                                                aggtggtAAAGGTATTAAAACAGCTACGATTACNGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGACACTATTCGACTAGATGTTGCAGATATTtc                                  >  1:751473/2‑141 (MQ=255)                                                                                                 ggtagtAAAGGTATTAAAACAGCTACGATTGCTGAGCATAATGATAATGTTGTATGTATCACGACAGTAACTGGTTAATAAGATTTAATGATGGTGACTAATTCAAGTGTCATTATTCGACTAGATGTTGCAGATATTtct                                 <  4:166212/141‑1 (MQ=255)                                                                                                  gtggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTtctc                                <  2:55907/141‑1 (MQ=255)                                                                                                  gtggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTtctc                                >  4:144465/1‑141 (MQ=255)                                                                                                   tggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCa                               >  2:572023/1‑141 (MQ=255)                                                                                                   tggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTACGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCa                               >  1:702176/1‑141 (MQ=255)                                                                                                     gtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTg                                            <  3:79312/126‑1 (MQ=255)                                                                                                     gtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTg                                            >  4:79312/1‑126 (MQ=255)                                                                                                     gtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCaaa                             <  2:233832/141‑1 (MQ=255)                                                                                                       aaaGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAAt                           <  2:328399/141‑1 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    >  3:125419/1‑141 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    >  4:160060/1‑141 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    <  4:148644/141‑1 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    <  2:431936/141‑1 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    <  2:107269/141‑1 (MQ=255)                                                                                                                aaaaCAGCTACGATTACTGACCATAATGGTAATGTTGTATGTATCACTCCAGTAACCGGCGAAGAAGATTTAATGATTGTTACTAATGCAGGCGCCACTATTCGCCTAGATGTTGCAGATATCTCCCCACATGCTCGTgcc                  >  2:604062/1‑140 (MQ=255)                                                                                                                    cAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTgccgcaa              >  4:315465/1‑140 (MQ=255)                                                                                                                    cAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGcac              >  3:59213/1‑141 (MQ=255)                                                                                                                      gCTACGATTACTGAGCATAGTGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACaa            <  2:24742/141‑1 (MQ=255)                                                                                                                      gCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACaa            >  2:164373/1‑141 (MQ=255)                                                                                                                       cTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAg           >  3:56405/1‑141 (MQ=255)                                                                                                                        tACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAgg          >  4:220702/1‑141 (MQ=255)                                                                                                                          cGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTGATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAGAATGGTCGTGCAGCACAAGgtg        >  1:714795/1‑141 (MQ=255)                                                                                                                          cGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGgtg        >  2:149357/1‑141 (MQ=255)                                                                                                                           gATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGgtgt       >  2:585854/1‑141 (MQ=255)                                                                                                                            aTTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGtt      >  3:200960/1‑141 (MQ=255)                                                                                                                             ttACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTc     <  4:150814/141‑1 (MQ=255)                                                                                                                              tACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCg    >  3:244010/1‑141 (MQ=255)                                                                                                                                cTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCGCt  >  2:741804/1‑141 (MQ=255)                                                                                                                                      |                                                                                                                                       GTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCGTAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCGCT  >  USA300TCH1516_ALE/9147‑9414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑