Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000731 81 N→G 100% intergenic (–/‑151)  / → repA –/replication protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000731810NG99.0% 287.6 / ‑5.3 91intergenic (–/‑151)–/repA–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (54/35);  minor base T (1/0);  total (55/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAG  >  CP000731/1‑219
                                                                                                                                           |                                                                                                                                          
gatatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGa                                                                                                                                           <  1:252934‑M1/82‑1 (MQ=255)
gatatttatcaagggtttataaaaccttatttaatctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGa                                                                                                                                           >  2:259201‑M1/60‑141 (MQ=255)
  tatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGaaa                                                                                                                                         <  2:86785‑M1/84‑1 (MQ=255)
  tatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGaaa                                                                                                                                         >  1:9766‑M1/58‑141 (MQ=255)
   atttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACCACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAAc                                                                                                                                        >  1:311840‑M1/57‑141 (MQ=255)
    tttatcaagagtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACt                                                                                                                                       >  1:10826‑M1/56‑141 (MQ=255)
     ttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTg                                                                                                                                      >  1:33360‑M1/55‑141 (MQ=255)
       atcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtt                                                                                                                                    >  1:37134‑M1/53‑141 (MQ=255)
        tcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGttt                                                                                                                                   <  2:74631‑M1/90‑1 (MQ=255)
           agggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttatt                                                                                                                                >  2:201004‑M1/49‑141 (MQ=255)
           agggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttatt                                                                                                                                <  1:99829‑M1/93‑1 (MQ=255)
            gggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAAATATCGGTGAAGGGTGTGGTTTGGTAATAAAACGTTTAGAAAATGattattt                                                                                                                               <  2:383823‑M1/94‑1 (MQ=255)
             ggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttattta                                                                                                                              >  2:15026‑M1/47‑141 (MQ=255)
              gtttataaacccttgataaagctatcttttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTttatttat                                                                                                                             >  1:181009‑M1/46‑141 (MQ=255)
              gtttataaacccttgataaagctatatttgcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTttatttat                                                                                                                             >  2:241102‑M1/46‑141 (MQ=255)
                 tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatata                                                                                                                          <  2:211268‑M1/99‑1 (MQ=255)
                    aaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGTTAATAAAACGTTTAGAAACTGTTTATTTATATAAtt                                                                                                                       <  1:340351‑M1/102‑1 (MQ=255)
                     aacccttgataaagctactttttcccttttataagtttACTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGTCGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                      >  2:57448‑M1/39‑141 (MQ=255)
                        ccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCg                                                                                                                   >  2:108400‑M1/36‑141 (MQ=255)
                         cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGa                                                                                                                  >  1:298530‑M1/35‑141 (MQ=255)
                           tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCAATa                                                                                                                <  2:102179‑M1/109‑1 (MQ=255)
                           tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTAGATa                                                                                                                <  1:227238‑M1/109‑1 (MQ=255)
                           tgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTACAAACTGTTTATTTATATAATTTTCGATa                                                                                                                <  2:26459‑M1/109‑1 (MQ=255)
                            gataaagctatattttcccttttataagtttATTTAAAGTGGTATCATCAACTTAATGTAGCAAAAACCCGAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATtttcgttat                                                                                                               <  1:302410‑M1/110‑5 (MQ=255)
                            gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAc                                                                                                               <  1:114780‑M1/110‑1 (MQ=255)
                            gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAc                                                                                                               >  2:206623‑M1/32‑141 (MQ=255)
                             ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                              <  2:367537‑M1/111‑1 (MQ=255)
                             ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                              >  1:379048‑M1/31‑141 (MQ=255)
                             ataaagctatattttaccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                              >  2:371443‑M1/31‑141 (MQ=255)
                               aaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTg                                                                                                            <  1:307327‑M1/113‑1 (MQ=255)
                                 agctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATACAATTTTCGATACTTGta                                                                                                          <  2:248362‑M1/115‑1 (MQ=255)
                                  gctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTACTTATATAATATTTTATACTtgtct                                                                                                         <  2:372149‑M1/116‑3 (MQ=255)
                                    tatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTAATTATATAATTTTCGATACTTGtatta                                                                                                       >  2:181572‑M1/24‑141 (MQ=255)
                                     atattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtattat                                                                                                      >  1:100363‑M1/23‑141 (MQ=255)
                                          ttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTAGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAAt                                                                                                 <  1:261708‑M1/124‑1 (MQ=255)
                                          ttcccttttataaggttATGTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAAt                                                                                                 >  2:18926‑M1/18‑141 (MQ=255)
                                           tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGAGATCTGCAAATAGATCTTTTAGAAACAGATGATGTATATAATTTTCGATACTTGTATTATATAATg                                                                                                <  2:160409‑M1/125‑1 (MQ=255)
                                              cttttattagtttCATTAAAGTGCTATAATAAACTTAATATAACAAAAACCATCTGTTCTGGTATTTGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaat                                                                                             >  2:99829‑M1/14‑141 (MQ=255)
                                                tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAGCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                           >  1:283663‑M1/12‑141 (MQ=255)
                                                tttataaatttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCTAACTACTGGTAATAGTCGGTGTTTGGTAATAAAATGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                           >  2:68824‑M1/12‑141 (MQ=255)
                                                  tataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCATCTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAataata                                                                                         >  2:290838‑M1/10‑141 (MQ=255)
                                                     aagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCACAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCt                                                                                      >  2:159909‑M1/7‑141 (MQ=255)
                                                            tttAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCa                                                                               >  1:194105/1‑141 (MQ=255)
                                                             ttAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCaa                                                                              >  2:217045/1‑141 (MQ=255)
                                                                aaGTGCTATATTATACTTAATATAACAAAAACCGCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTTCAAGcc                                                                           >  2:264907/1‑140 (MQ=255)
                                                                 aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                          <  2:158136/141‑1 (MQ=255)
                                                                  gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGACTAATATCTTATTGCAATCAcc                                                                         >  2:136328/1‑141 (MQ=255)
                                                                  gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGACAGGGTGCGATAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATAAATAATATATTAATGCAAGCAcc                                                                         >  2:162332/1‑141 (MQ=255)
                                                                  gTGCAATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                         <  1:45469/141‑1 (MQ=255)
                                                                  atGCTATAATAAACTGAATATAACAAAAACACCAACTAGTTTTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                         <  2:238955/140‑1 (MQ=255)
                                                                    gCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTa                                                                       >  2:42184/1‑141 (MQ=255)
                                                                     cTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTCGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATACTGCTTAATATCTTATTGCATGCACCCaa                                                                      >  2:101661/1‑141 (MQ=255)
                                                                     atataATAAACATAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTaa                                                                      <  2:322815/140‑1 (MQ=255)
                                                                         aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                                  >  1:286082/1‑141 (MQ=255)
                                                                         aataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCa                                                                  >  1:361345/1‑141 (MQ=255)
                                                                           taaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATATTAGTGCAAGCACCTAAATCAtt                                                                >  1:9322/1‑141 (MQ=255)
                                                                            aaaCTTAATATAACAAAAACCCCCACTACCAATAATTTTTGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATta                                                               <  1:168279/141‑1 (MQ=255)
                                                                              aCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaa                                                             >  1:330383/1‑141 (MQ=255)
                                                                              aCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaa                                                             <  1:86785/141‑1 (MQ=255)
                                                                               cTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaat                                                            <  1:174935/141‑1 (MQ=255)
                                                                                 tAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATCATTGTATTAGAGAATGAATAATAACTTAGTGCAAGCACCTAAATCATtaaataa                                                          >  1:329713/1‑141 (MQ=255)
                                                                                     ataACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAATGGAAGCACCTAAATCATTAAATAATTGt                                                      >  2:154103/1‑141 (MQ=255)
                                                                                         cAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAg                                                  >  1:179340/1‑141 (MQ=255)
                                                                                            aaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCta                                               <  1:231863/141‑1 (MQ=255)
                                                                                            aaaCCCCAACTACTGGTAATAGTCAGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCta                                               <  1:237996/141‑1 (MQ=255)
                                                                                             aaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaa                                              >  2:355899/1‑141 (MQ=255)
                                                                                             aaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTACAAGCACCTAAATCATTAAATAAATGTAAAGCtaa                                              <  2:331634/141‑1 (MQ=255)
                                                                                              aCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGCTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaa                                             >  2:307244/1‑141 (MQ=255)
                                                                                               ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                            >  1:155818/1‑141 (MQ=255)
                                                                                               ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                            >  2:129453/1‑141 (MQ=255)
                                                                                               ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                            >  2:334061/1‑141 (MQ=255)
                                                                                                cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                           >  1:347379/1‑141 (MQ=255)
                                                                                                cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                           <  1:319907/141‑1 (MQ=255)
                                                                                                 ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                          <  1:321088/141‑1 (MQ=255)
                                                                                                  cAACTACTGAAACTACTCTGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataaa                                         >  2:145121/1‑141 (MQ=255)
                                                                                                     ctactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAAc                                      >  2:24853/1‑141 (MQ=255)
                                                                                                      tactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTATAAACGGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                     >  1:76819/1‑141 (MQ=255)
                                                                                                      tactGGTAATAGTCGGGGTTTGGTAATAAAACGTTCAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                     >  1:26644/1‑141 (MQ=255)
                                                                                                         tGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGttt                                  >  2:378390/1‑141 (MQ=255)
                                                                                                                  tCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATCTAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACctc                         <  2:209773/141‑1 (MQ=255)
                                                                                                                  tCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACctc                         >  2:219970/1‑141 (MQ=255)
                                                                                                                     gggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTa                      <  1:86628/141‑1 (MQ=255)
                                                                                                                          tGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                 <  1:324553/141‑1 (MQ=255)
                                                                                                                          tGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                 >  1:44641/1‑141 (MQ=255)
                                                                                                                          tGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGAACATAAATAATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                 >  2:341681/1‑141 (MQ=255)
                                                                                                                               ataaAACGTTTAGAAACTGTTTATTTATATAATATTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTATTACCAAACTaaa            >  2:146052/1‑141 (MQ=255)
                                                                                                                               ataaAACGTTTAGAAACTGTTTATTTATATAAATATCGATACTTGTATTATATAATGAATAATACCTTCGCGTATTTTCCTAAATGTATTCATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaaa            >  1:353294/1‑141 (MQ=255)
                                                                                                                                  aaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTAATATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGATTTATTACTTGTTACCAAACTAAAata         <  1:209309/141‑1 (MQ=255)
                                                                                                                                       tttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaa             >  2:26878/1‑132 (MQ=255)
                                                                                                                                       tttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaa             <  1:26878/132‑1 (MQ=255)
                                                                                                                                         tAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAag  <  2:212376/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAG  >  CP000731/1‑219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: