Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 614,199 T→C 7.3% D837D (GAT→GAC sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE614,1990TC 7.3% 499.7 / 4.6 175D837D (GAT→GACsdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base T (81/79);  new base C (2/13);  total (83/92)
Fisher's exact test for biased strand distribution p-value = 6.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATAGCGATT  >  USA300TCH1516_ALE/614059‑614338
                                                                                                                                            |                                                                                                                                           
gattcagattcaGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                                                             <  1:786901/141‑1 (MQ=255)
    cagattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                                                         <  3:331006/141‑1 (MQ=255)
    cagattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                                                         >  1:307064/1‑141 (MQ=255)
      gactcagatTCCGATTCGGACTCAGACACTGACGCAGCCACAGACTCAGATAGCGACTCAGATTCTGACACCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACtcagattcagat                                                                                                                                       <  2:1065550/138‑1 (MQ=17)
        ttcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatag                                                                                                                                     <  3:464815/141‑1 (MQ=255)
          cagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCCGACAGCTTCTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcg                                                                                                                                   >  1:104240/1‑141 (MQ=255)
           agatAGCGATTCCGACTCAGACAGTGATTCAGAATCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcga                                                                                                                                  <  3:671277/141‑1 (MQ=255)
              tAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactc                                                                                                                               <  2:390243/141‑1 (MQ=255)
                gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTAAGATTCAGACAGCGACTCAGATtcagatagcgactcag                                                                                                                             >  1:1575786/1‑141 (MQ=255)
                  gATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagac                                                                                                                           >  4:153053/1‑141 (MQ=255)
                    ttCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactc                                                                                                                         >  4:680610/1‑141 (MQ=255)
                    ttCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcga                                                                                                                                  <  2:272676/132‑1 (MQ=255)
                    ttCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGAATCAGACAACGACTCAGATtcaaatagcgactcaaacac                                                                                                                         >  4:369808/1‑139 (MQ=255)
                        gACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagat                                                                                                                     >  1:999690/1‑141 (MQ=255)
                        gACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagat                                                                                                                     <  1:1199158/141‑1 (MQ=255)
                        gACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagag                                                                                                                     >  2:1432379/1‑140 (MQ=255)
                          cTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACCCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatag                                                                                                                   <  2:723346/141‑1 (MQ=255)
                           ccAGACTGCGACTCAGATTCAGACTCAGATCGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagc                                                                                                                  <  2:1468474/140‑1 (MQ=255)
                           acAGACAGTGATTCAGATTCAGACTCAGATACCGACTCAGATTCTGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagc                                                                                                                  <  2:649252/140‑1 (MQ=255)
                             aGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcga                                                                                                                >  1:682422/1‑141 (MQ=255)
                             aGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcga                                                                                                                >  1:479215/1‑141 (MQ=255)
                              gACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcaggtagcgactcagactcagatagcgac                                                                                                               <  1:1146740/141‑1 (MQ=255)
                                cAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactc                                                                                                             <  1:1475736/141‑1 (MQ=255)
                                cAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactc                                                                                                             >  2:770937/1‑141 (MQ=255)
                                 ggTGATTCAGATTCAGACGCAGAGAGCGACTCAGATTCTGACAGCGATTCAGGCTCAGACAGCGACTCAGGCTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactca                                                                                                            <  1:973606/140‑1 (MQ=255)
                                  ctGATTCAGATCCAGACTCCGATAGCGACTCAGATTCCGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcag                                                                                                           <  2:1185340/140‑1 (MQ=255)
                                   tGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcaga                                                                                                          >  4:170855/1‑141 (MQ=255)
                                    gattcagattcaGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGTTTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagac                                                                                                         >  2:1151541/1‑141 (MQ=255)
                                      ttcagattccgaCTCAGATACCGACTCAGATTCAGACACCGATTCAGAATCCGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACtcagatagcgactcagactcagatagcgactcagactc                                                                                                       <  2:1411794/141‑1 (MQ=14)
                                        cagattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcag                                                                                                     >  1:5471/1‑141 (MQ=255)
                                          gattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagat                                                                                                   >  1:1265893/1‑141 (MQ=255)
                                            ctcagaCTCAGATACCGACTCAGATTCTGACTGTGATTCAGACTCAGACAGCGACCCAGACTCAGACACGGACTCAGACTCAGACAGCGACTCAGACtcagacagcgactcagactcagatagcgactcagactcagatag                                                                                                 <  2:597685/140‑1 (MQ=14)
                                              cagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcg                                                                                               >  2:1203522/1‑141 (MQ=255)
                                               agaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcggatagcgactcagactcagatagcgactcagactcagatagcga                                                                                              <  1:1435304/141‑1 (MQ=255)
                                                  cTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactc                                                                                           >  1:283139/1‑141 (MQ=255)
                                                    cAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactcag                                                                                         <  1:1542519/141‑1 (MQ=255)
                                                    cAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactcag                                                                                         >  2:1250262/1‑141 (MQ=255)
                                                    cAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactcag                                                                                         <  1:1232033/141‑1 (MQ=255)
                                                      gATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactcagac                                                                                       <  3:587980/141‑1 (MQ=255)
                                                        tAGCGACTCAGATTCTGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATTCagatagcgactcagactcagatagcgactcagactcagatagcgactcagactc                                                                                     <  2:421645/141‑1 (MQ=255)
                                                        tAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCagatagcgactcagactcagatagcgactcagactcagatagcgactcagactc                                                                                     >  2:18068/1‑141 (MQ=255)
                                                         aGCGACTCCGACTCTGATAGCGATTCAGACTCAGACAGCGCCTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCg                                                                                    <  2:1031678/141‑1 (MQ=14)
                                                          gCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCgg                                                                                   >  1:840035/1‑141 (MQ=255)
                                                          ccGACTCAGATTCTGACAGCGATCCAGAGCGAAACAGCGACTCAGACTCAGACAGCGAACCAGACTCAGACAGCAACTCAGACTCATACGGCGACGCGAAACCAGATATCGAAACAGACCCGGATAGCGACTCAGACACgg                                                                                   >  4:292718/2‑141 (MQ=2)
                                                           cGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGa                                                                                  <  3:323694/141‑1 (MQ=255)
                                                           cGACCCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACACAGACTCAGATAGCGACTCAGACTCGGa                                                                                  <  4:486919/141‑1 (MQ=255)
                                                            gACTCAGACTCTGACAGAGATTCAGACTCAGACAGCGACTCAGACTCAGACAGAGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGAt                                                                                 <  2:804660/141‑1 (MQ=255)
                                                              cccAGATTCTGCCTGCGATCCAGACTCAGACAGCGACTCAGCCTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAg                                                                               <  2:283610/139‑1 (MQ=17)
                                                                  gATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgat                                                                           <  1:1271182/141‑1 (MQ=255)
                                                                      cTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcag                                                                       <  1:1333245/141‑1 (MQ=255)
                                                                        gACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagat                                                                     >  1:1032500/1‑141 (MQ=255)
                                                                          cAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGCTTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCCGATAGCGACTCAGACTCCGATAGCgattcagatac                                                                   >  2:894323/1‑139 (MQ=255)
                                                                          cAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagattc                                                                   <  1:169777/141‑1 (MQ=255)
                                                                          cAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagattc                                                                   <  1:561741/141‑1 (MQ=255)
                                                                             ccgttCAGACTCAGACCGAGACTCAGACGCTCACAGCGATTCAGATTCAGACAGCGGCTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGAttcagattcaga                                                                <  2:1377175/138‑1 (MQ=14)
                                                                              gATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACCCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGCCTCGGATAGCGATCCAGATCCAGAc                                                               >  4:112807/1‑141 (MQ=255)
                                                                               aTTCAGACTCAGATTCCGTCTCAGACTCAGACACGGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACa                                                              <  2:747420/141‑1 (MQ=17)
                                                                                ttCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAg                                                             <  1:323447/141‑1 (MQ=255)
                                                                                 tcagactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGc                                                            >  1:1642269/1‑141 (MQ=255)
                                                                                  cagactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATGCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCg                                                           <  3:680610/141‑1 (MQ=255)
                                                                                    gactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGAc                                                         >  3:41248/1‑141 (MQ=255)
                                                                                    gactcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGAc                                                         >  1:847415/1‑141 (MQ=255)
                                                                                      ctcagacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtc                                                       >  1:88427/1‑141 (MQ=255)
                                                                                         agacAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGGTAGCGACTCAGGCTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcaga                                                    <  1:902075/141‑1 (MQ=255)
                                                                                               cGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcaga                                              <  3:456162/141‑1 (MQ=255)
                                                                                               cGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcaga                                              <  4:555167/141‑1 (MQ=255)
                                                                                               cGACTCAGACTCACACAGTGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcaga                                              <  2:850053/141‑1 (MQ=11)
                                                                                                gactcagactcaGACAGTGATTCCGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACACAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcagat                                             <  2:615981/141‑1 (MQ=255)
                                                                                                gactcagactcaGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattcagat                                             >  1:1529280/1‑141 (MQ=255)
                                                                                                gactcagactcaGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGAGATTCAGATTCAGACAGCGACacagattcagag                                             >  4:297505/1‑140 (MQ=255)
                                                                                                  ctcagactcagaCAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCCGATaa                                           >  2:491035/1‑140 (MQ=255)
                                                                                                   tcagactcagacAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGAGAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGc                                          <  4:210164/141‑1 (MQ=255)
                                                                                                    cagactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCg                                         >  3:220135/1‑141 (MQ=255)
                                                                                                     agactcagacAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcga                                                                                              >  2:1236387/1‑87 (MQ=255)
                                                                                                      gactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGCCTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGAAAGCGACTCAGATTCAGATAGCGAt                                       >  2:561691/1‑141 (MQ=255)
                                                                                                      gactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTCACGCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt                                       >  1:1606197/1‑141 (MQ=255)
                                                                                                      gactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt                                       >  1:1598128/1‑141 (MQ=255)
                                                                                                      gactcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt                                       <  3:297367/141‑1 (MQ=255)
                                                                                                        ctcagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTc                                     >  1:702100/1‑141 (MQ=255)
                                                                                                          cagacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                   >  4:26346/1‑141 (MQ=255)
                                                                                                           agacAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGGCTCTGCCTCGGATAGCGCTTCAGATTCAGACAGCGCCTCAGATTCAGATAGCGATTCGGa                                  <  2:1589756/141‑1 (MQ=14)
                                                                                                                 tGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                            <  1:109283/141‑1 (MQ=255)
                                                                                                                    ttcagattcagaCAGCGACTCAGATtcagatagcgactcagactcagatagcgactc                                                                                                             <  2:1508961/57‑1 (MQ=255)
                                                                                                                    ttcagattcagaCAGCGACTCAGATtcagatagcgactcagactcagatagcgactc                                                                                                             >  1:1508961/1‑57 (MQ=255)
                                                                                                                    ttcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACACAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACaa                         <  1:1121026/141‑1 (MQ=255)
                                                                                                                     tcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAAc                        >  1:362783/1‑141 (MQ=255)
                                                                                                                      cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACg                       >  1:1430496/1‑141 (MQ=255)
                                                                                                                      cagattcagaCAGCGACTCAGACTCGGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACg                       <  3:109453/141‑1 (MQ=14)
                                                                                                                        gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGACTTAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGAc                     >  1:140643/1‑141 (MQ=255)
                                                                                                                                cAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACtcagattc             <  3:173335/141‑1 (MQ=255)
                                                                                                                                 aGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattca            <  2:479215/141‑1 (MQ=255)
                                                                                                                                     aCTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATa        >  2:1369237/1‑141 (MQ=255)
                                                                                                                                      cTCAGATTAAGACAGCGGCTCAGACTCAGATAGCGACCCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATAg       <  1:425491/141‑1 (MQ=14)
                                                                                                                                       tcagattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACtcag                 >  3:656603/1‑130 (MQ=255)
                                                                                                                                        cagattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATAGCg     >  1:592610/1‑141 (MQ=255)
                                                                                                                                        cagattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATAGCg     >  1:1166727/1‑141 (MQ=255)
                                                                                                                                        cagattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGACAGCg     >  4:624077/1‑141 (MQ=255)
                                                                                                                                          gattcagatagcgactcagactcagatagcgactcagactcaga                                                                                                    <  1:264260/44‑1 (MQ=255)
                                                                                                                                          gattcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGAGAGCGAATCAGATGTAGACATCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATAGCgat   >  1:990170/1‑141 (MQ=17)
                                                                                                                                           attcagatAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGATTCAGACAACGCATCAGATTCAGATAGAgatt  >  4:674196/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTCAGATTCAGATAGCGATT  >  USA300TCH1516_ALE/614059‑614338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: