Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,464 G→A 5.4% S1009S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4640GA 5.4% 491.4 / 3.1 182S1009S (TCG→TCAsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (81/83);  new base A (8/5);  total (89/93)
Fisher's exact test for biased strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 187 total aligned reads displayed.

GACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622327‑622604
                                                                                                                                         |                                                                                                                                            
 tcagTGCCTCAGCGTCGGACAGCGGTTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACt                                                                                                                                          <  2:573012/140‑1 (MQ=11)
  cagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                         <  1:566016/141‑1 (MQ=255)
  cagTGATTCAGATTCAGACAGCGACACAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                         <  2:603213/141‑1 (MQ=255)
     tGATTCAGATACAAACAGCTACTCATATTCAGATAGCGACTCAGATTCAGACATCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCACACAGCGACTCAGATTCAGATATCGATTCGGACTCAGa                                                                                                                                      <  4:101572/141‑1 (MQ=14)
      gattcagattcaGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAc                                                                                                                                     >  1:775002/1‑141 (MQ=255)
      gattcagattcaGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAc                                                                                                                                     >  1:730086/1‑141 (MQ=255)
        ttcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg                                                                                                                                   <  3:493111/141‑1 (MQ=255)
          cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                                                                                       >  3:393848/1‑135 (MQ=255)
          cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACACAGACAGCGACTCAGATGCAGAGAAAGATTCAGACCCAGACAGCg                                                                                                                                 >  2:773202/1‑141 (MQ=14)
            gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                               >  1:139280/1‑141 (MQ=255)
                cagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAAACACCGATACAg                                                                                                                           >  2:31385/1‑141 (MQ=14)
                      gCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAg                                                                                                                     >  1:1346086/1‑141 (MQ=255)
                       cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAATCAGa                                                                                                                    >  1:702/1‑141 (MQ=255)
                          cTCAGATTCAGATAGCGGCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                 <  1:1087507/141‑1 (MQ=255)
                          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCGGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                 >  1:54715/1‑141 (MQ=255)
                          cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                 <  1:1004952/141‑1 (MQ=255)
                           tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                <  1:225496/141‑1 (MQ=255)
                            cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                               <  4:255132/141‑1 (MQ=255)
                            cagattcagatAGCGACTCAGATTCGGACAGCGAGTCACGCTCACCACGCGGCTCTGCTCCAGATAGCGATTCAGACCCAGACAGCGCGTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                               <  2:779150/141‑1 (MQ=14)
                            cagattcagatAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCCGACAGCGACTCAGATCCAGATAGCGATTCGGACACAGACCGGGATTCAGACTCAGGGAGCg                                                                                                               >  2:969174/1‑141 (MQ=14)
                             agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATACAGATTCAGACAGAGATTCAGCCTCAAATAGCga                                                                                                              >  2:1293880/1‑141 (MQ=14)
                              gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                            <  1:1047015/110‑1 (MQ=255)
                              gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                             <  1:181890/141‑1 (MQ=255)
                              gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                             >  1:631862/1‑141 (MQ=255)
                               attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                                                                            <  2:426394/141‑1 (MQ=255)
                                  cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                         <  1:483311/141‑1 (MQ=255)
                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                       <  2:70490/141‑1 (MQ=255)
                                    gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                       >  1:1111693/1‑141 (MQ=255)
                                      tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                     <  1:410945/141‑1 (MQ=255)
                                        gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                                   >  3:285513/1‑141 (MQ=255)
                                         cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                                                  <  1:1410107/141‑1 (MQ=255)
                                               aGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                            <  2:1365275/141‑1 (MQ=255)
                                                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           <  1:1203571/141‑1 (MQ=255)
                                                gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           <  3:443118/141‑1 (MQ=255)
                                                  ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                         <  1:855337/141‑1 (MQ=255)
                                                   tcagACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCGGATAGCGACtcg                                                                                        <  2:1101102/138‑2 (MQ=255)
                                                     ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcaga                                                                                      <  3:520219/141‑1 (MQ=255)
                                                        cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattc                                                                                   >  1:943053/1‑141 (MQ=255)
                                                        cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagacac                                                                                   >  4:604663/1‑138 (MQ=255)
                                                        aaGCGATTCAGACGCAGATAGCGACTCAGATTCAGATAGCGATGCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATGGCGACTCAGACTCAGACAGCGACtcagattc                                                                                   <  2:434828/140‑1 (MQ=255)
                                                          gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                 >  2:1572325/1‑141 (MQ=255)
                                                            gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                                                                               <  3:565236/141‑1 (MQ=255)
                                                              ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAg                                                                             >  2:1452894/1‑141 (MQ=255)
                                                              ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAg                                                                             >  4:191621/1‑141 (MQ=255)
                                                                  gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                         >  2:1631452/1‑141 (MQ=255)
                                                                    cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                       <  3:324649/141‑1 (MQ=255)
                                                                      cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                     <  2:730086/141‑1 (MQ=255)
                                                                      cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGAGAGTCAGACTGAGAGAGCGACTCAGATTCAGATAGCGATTCgg                                                                     >  2:398393/1‑141 (MQ=255)
                                                                       agacagcgactcagactcagacagcgactcagactcAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                    <  4:637222/141‑1 (MQ=255)
                                                                        gATAGCGACTCAGATTAAGATAGCGATTCAGCCTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                   <  1:1479546/141‑1 (MQ=255)
                                                                           aGCGACTCACACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                <  4:506770/141‑1 (MQ=11)
                                                                            gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACCGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGCAACTCAGAGTCAGACAGCGACTCGGACTCAg                                                               >  1:398060/1‑141 (MQ=14)
                                                                            gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                               >  3:679582/1‑141 (MQ=255)
                                                                            gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAg                                                               >  2:1115504/1‑141 (MQ=255)
                                                                                cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAg                                                           >  2:1328877/1‑141 (MQ=255)
                                                                                cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGAGTCGGACTCagagag                                                           >  2:1157655/1‑141 (MQ=255)
                                                                                 tcagatgcagatAGCGATTCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                          <  4:317273/141‑1 (MQ=255)
                                                                                  cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                         >  1:103034/1‑141 (MQ=255)
                                                                                   agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                        <  2:1357314/141‑1 (MQ=255)
                                                                                   agattcagacAGCGATTAAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                        <  2:903661/141‑1 (MQ=255)
                                                                                    gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGAc                                                       >  2:1084110/1‑141 (MQ=255)
                                                                                    gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                         >  1:821417/1‑139 (MQ=255)
                                                                                    gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                         <  2:821417/139‑1 (MQ=255)
                                                                                    gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                       <  4:513465/141‑1 (MQ=255)
                                                                                      ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                     >  1:1204396/1‑141 (MQ=255)
                                                                                       tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                    >  1:870623/1‑141 (MQ=255)
                                                                                        cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                   <  1:705145/141‑1 (MQ=255)
                                                                                          gatAGCGATTCAGTCTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAt                                                 >  1:189544/1‑141 (MQ=255)
                                                                                             aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGACACAGACAGCGACTCAGATCCAGAGAGCGACTCGGACTCAGACAGCGACTCAGacaca                                              >  2:297258/1‑137 (MQ=14)
                                                                                              gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAg                                             >  4:169527/1‑141 (MQ=255)
                                                                                               cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                            <  2:227955/141‑1 (MQ=255)
                                                                                                       actcagatAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATACCCATTCGGACTCAGATCGACACTCAGATTCAAACACCGact                                    >  4:61235/1‑139 (MQ=11)
                                                                                                        ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                           <  2:952136/69‑1 (MQ=255)
                                                                                                        ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                           >  1:952136/1‑69 (MQ=255)
                                                                                                          cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                 >  1:934652/1‑141 (MQ=255)
                                                                                                            gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                               <  1:1079048/141‑1 (MQ=255)
                                                                                                             ccAGCGACTCAGATTCAGAACGCGATTCTGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGCCTCAGATTCAGACAGCGATTCAGACt                              <  2:49778/140‑1 (MQ=14)
                                                                                                              cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                             >  3:565969/1‑141 (MQ=255)
                                                                                                              cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                             >  1:791593/1‑141 (MQ=255)
                                                                                                              cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                             <  2:730677/141‑1 (MQ=255)
                                                                                                                gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                           >  2:266033/1‑141 (MQ=255)
                                                                                                                 cGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                          <  2:1006821/141‑1 (MQ=255)
                                                                                                                  gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                         >  2:796447/1‑141 (MQ=255)
                                                                                                                  cactcagactcagACAGCGCGTCGGACTCAGACTCTGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                         <  2:1185904/140‑1 (MQ=14)
                                                                                                                    cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       >  1:1321208/1‑141 (MQ=255)
                                                                                                                      cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGATTCAGAAAGCGACTCAGATTCAGAAAGAGACTCAGACTCCGATAGcc                     >  4:622461/1‑140 (MQ=14)
                                                                                                                       agcttcagatAGCGGTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                    <  4:68100/138‑1 (MQ=255)
                                                                                                                        gattcagatACCGACGCGGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                   <  4:388076/141‑1 (MQ=14)
                                                                                                                          ttcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                 >  4:308087/1‑141 (MQ=255)
                                                                                                                             agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga              <  1:1119414/141‑1 (MQ=255)
                                                                                                                                tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGCTTCGGAGTCAGTTAGCGGCTCAGATTCAGACAGCGGTTCAGACTCAGATAGCgactcagcctc           >  3:203139/1‑141 (MQ=17)
                                                                                                                                tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc           >  1:581669/1‑141 (MQ=255)
                                                                                                                                cagtgattcagactcagatagcgattcagactcagatagcGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc           >  2:1285610/5‑141 (MQ=255)
                                                                                                                                caGCGATTGTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc           <  4:575941/140‑1 (MQ=255)
                                                                                                                                  gCGATGCGGACGCAGAAAGCGCCTAAGACTCAGATATCGACGCAGACTCAGACAGCGACTCAGATTCAGAAAGCGACTCGGACTCAGACAGCGACCCAGAGTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag         <  4:417747/141‑1 (MQ=14)
                                                                                                                                    gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac       <  1:515326/141‑1 (MQ=255)
                                                                                                                                        cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg   >  3:446370/1‑141 (MQ=255)
                                                                                                                                         ggACTCAGCAAGCGATGCAGACTCAGATAGCGAATCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga  <  2:887288/141‑1 (MQ=17)
                                                                                                                                         ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga  <  1:1600242/141‑1 (MQ=255)
                                                                                                                                         ggACTCAGACAGCGATTCAGACGCAGATAGCGCCTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga  <  4:469497/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622327‑622604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: