Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,569 T→C 5.7% D1044D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5690TC 5.7% 480.1 / 2.9 170D1044D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (83/75);  new base C (9/3);  total (92/78)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

GACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAGTGATTCAGACAGCGATTC  >  USA300TCH1516_ALE/622429‑622709
                                                                                                                                            |                                                                                                                                            
gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                                                                              <  2:464268/141‑1 (MQ=255)
 attcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                                                             >  2:804295/3‑141 (MQ=255)
    cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                                                          >  1:529375/1‑141 (MQ=255)
    cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                                                          >  1:934652/1‑141 (MQ=255)
       ccAGCGACTCAGATTCAGAACGCGATTCTGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGCCTCAGATTCAGACAGCGATTCAGACt                                                                                                                                       <  2:49778/140‑1 (MQ=14)
        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                                                      >  1:791593/1‑141 (MQ=255)
        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                                                      >  3:565969/1‑141 (MQ=255)
        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                                                      >  1:134269/1‑141 (MQ=255)
        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                                                      <  2:730677/141‑1 (MQ=255)
          gCGACTCAGATTCAGATAGCGATTCGGACTCAGAGAGCGCTTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCTAATACAGACAGCGACGCAGACTCAg                                                                                                                                    >  2:575989/1‑141 (MQ=14)
           cGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                                                                   <  2:1006821/141‑1 (MQ=255)
           cGACTCAGATGCAGATAGCGATGCAGACTCAGACAGCGATGCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                                                                   <  2:288903/141‑1 (MQ=14)
              cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                                                                >  1:1321208/1‑141 (MQ=255)
                 agcttcagatAGCGGTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                                                                                                                             <  4:68100/138‑1 (MQ=255)
                    ttcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                          <  2:791593/141‑1 (MQ=255)
                       agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                                                                                       <  1:1119414/141‑1 (MQ=255)
                       agacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACACAGATAGCGACTCAGATTCAGACAGCGACACAGACCCAGATAGCgactcaga                                                                                                                       >  2:257799/1‑141 (MQ=11)
                        gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                                      >  1:637297/1‑141 (MQ=255)
                          tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGCTTCGGAGTCAGTTAGCGGCTCAGATTCAGACAGCGGTTCAGACTCAGATAGCgactcagcctc                                                                                                                    >  3:203139/1‑141 (MQ=17)
                          tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcagactc                                                                                                                    >  1:1648897/1‑141 (MQ=255)
                          tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                                    >  1:581669/1‑141 (MQ=255)
                          caGCGATTGTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                                    <  4:575941/140‑1 (MQ=255)
                            gtgaggcGGACTCACACACCGCCTCAGACGCAGAGAGCGACTCAGACTCAGACAGCGACTAAGAGTCAGACAGCGACTCAGAATCAGATAGAGATACAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                                                  <  4:630924/135‑1 (MQ=11)
                            gCGATTCGGACGCAGACAGCGAGGCAGATGAAGATAGCGCATCTGACTAAGACAGCGCCTCAGACTCAGCCAGCGCTTCGGACTCAGATAGTGACTCTGATTCAGACAGCGATTCAGACTCAGATAGCGactcatactcag                                                                                                                  <  4:417886/141‑1 (MQ=12)
                              gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                                <  1:515326/141‑1 (MQ=255)
                                ttCGGACTCAGACAGCGATTCAGACGCAGATAGCGATGCATGGTCAGGCAACGGCTCAGATTCAGATAGCTATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                                                              <  1:1073547/141‑1 (MQ=14)
                                  cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                                                            >  3:446370/1‑141 (MQ=255)
                                   ggACTCAGCAAGCGATGCAGACTCAGATAGCGAATCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                                                           <  2:887288/141‑1 (MQ=17)
                                   ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                                                           <  1:1600242/141‑1 (MQ=255)
                                   ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                                                           <  1:1495521/141‑1 (MQ=255)
                                   ggACTCAGACAGCGATTCAGACGCAGATAGCGCCTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                                                           <  4:469497/141‑1 (MQ=255)
                                     aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt                                                                                                         >  2:1032420/1‑141 (MQ=255)
                                      cTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattc                                                                                                        >  3:122724/1‑141 (MQ=255)
                                        cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTAAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag                                                                                                      >  1:1436353/1‑141 (MQ=255)
                                         aGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                                                                     >  2:811447/1‑141 (MQ=255)
                                          gACAGCGATTCCGACTCAGATAGCGACTCAGCCTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat                                                                                                    <  4:382740/141‑1 (MQ=255)
                                          gACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGACGGCgaatcagaa                                                                                                    >  2:224717/1‑140 (MQ=11)
                                            cAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                                                                  <  2:775002/141‑1 (MQ=255)
                                              gCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagactgcg                                                                                                >  2:399716/1‑136 (MQ=255)
                                                    cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCg                                                                                          >  4:389539/1‑141 (MQ=255)
                                                    cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACACAGATACAGACAAAGACTCAGACTCAGATAGCGAATAAGATGAAGACAGTGAATCAGATTCAGACAACg                                                                                          >  4:305583/1‑141 (MQ=14)
                                                      gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGATAGCgaa                                                                                        >  4:269332/1‑140 (MQ=255)
                                                        cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                                                      >  1:639045/1‑141 (MQ=255)
                                                        cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                                                      >  2:713930/1‑141 (MQ=255)
                                                          cAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcag                                                                                    >  1:285715/1‑141 (MQ=255)
                                                           aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcaga                                                                                   >  2:1555967/1‑141 (MQ=255)
                                                           aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcaga                                                                                   >  1:1158503/1‑141 (MQ=255)
                                                            gATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGAGTCAGACAGCgactcagac                                                                                  >  4:306758/1‑141 (MQ=255)
                                                               aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactca                                                                               >  2:867328/1‑141 (MQ=255)
                                                               aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactca                                                                               <  2:139280/141‑1 (MQ=255)
                                                                    ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAg                                                                          >  4:561670/1‑141 (MQ=255)
                                                                      cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATaacg                                                                        >  1:916821/1‑138 (MQ=255)
                                                                        gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGAc                                                                      >  3:562626/1‑141 (MQ=255)
                                                                        gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGAc                                                                      <  1:429815/141‑1 (MQ=255)
                                                                          ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACCCAGACTCAGATAACGAATc                                                                    >  2:1323989/1‑141 (MQ=255)
                                                                            cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAg                                                                  <  1:246575/141‑1 (MQ=255)
                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                                                              >  1:376613/1‑141 (MQ=255)
                                                                                cAGCGACGCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                                                              <  2:1592115/141‑1 (MQ=14)
                                                                                  gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                                                            >  4:431214/1‑141 (MQ=255)
                                                                                  gCGACTCAGATTCAGATAGAGATTCGGACTCAGATAGCGACTCAGAATCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                                                            <  3:169527/141‑1 (MQ=255)
                                                                                   cGACTCCGATTCAGAAAGCGACTCAGACTCAGACAGCGACTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                                                           <  2:1409291/141‑1 (MQ=14)
                                                                                   cGACTCAGAGTCCGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                                                           <  4:541061/141‑1 (MQ=255)
                                                                                    gACTCAGATTTAGATAGCGATTCGGACTCTGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTTAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                                          <  3:611095/141‑1 (MQ=255)
                                                                                    gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                                          >  2:650602/1‑141 (MQ=255)
                                                                                    gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                                          >  3:459858/1‑141 (MQ=255)
                                                                                    gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                                          >  1:1069673/1‑141 (MQ=255)
                                                                                      cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAg                                                        >  4:640570/1‑141 (MQ=255)
                                                                                      cTCAGATGCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACacagacag                                                        >  2:1151825/1‑141 (MQ=255)
                                                                                       tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGc                                                       <  2:1204396/141‑1 (MQ=255)
                                                                                        cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCg                                                      >  1:1174582/1‑141 (MQ=255)
                                                                                          gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGAc                                                    >  2:399807/1‑141 (MQ=255)
                                                                                               agatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCTGACAGCGACTCAGa                                               <  3:308087/141‑1 (MQ=255)
                                                                                                   aGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGCCTCAGACTCAGACAGTGATTCAGCATCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActct                                           <  1:746800/141‑1 (MQ=255)
                                                                                                    gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                          >  1:278735/1‑61 (MQ=255)
                                                                                                    gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                          <  2:278735/61‑1 (MQ=255)
                                                                                                    gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACCGCGACTCAGACTCTg                                          >  1:841208/1‑141 (MQ=255)
                                                                                                    gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg                                          >  1:841025/1‑141 (MQ=255)
                                                                                                    gCGACTCCGACTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg                                          <  2:752556/141‑1 (MQ=14)
                                                                                                        ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg                                      <  1:96345/141‑1 (MQ=255)
                                                                                                        ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg                                      <  2:1168798/141‑1 (MQ=255)
                                                                                                         tCGGACTCAGATAGCGACTCAGCTTCAGACAGCGATTCAGCCTCAGCTCGCCAATCAGATTCAGCCCGCGCTTCAGGTCCAGACCGCGACTCTGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGc                                     <  1:327789/141‑1 (MQ=17)
                                                                                                         tCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGc                                     <  1:1230968/141‑1 (MQ=255)
                                                                                                          cGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTAAGCTAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCg                                    >  3:6408/1‑141 (MQ=255)
                                                                                                            gACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgac                                  >  4:564220/1‑141 (MQ=255)
                                                                                                             aCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgact                                 >  3:153841/1‑141 (MQ=255)
                                                                                                                cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcag                              >  3:397237/1‑141 (MQ=255)
                                                                                                                cAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcag                              >  2:457604/1‑141 (MQ=255)
                                                                                                                 aGATAGCGACTCAGATTCAGACAGCGATTCAGACTCATATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcaga                             >  1:1194077/1‑141 (MQ=255)
                                                                                                                 aGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcaga                             <  1:1528164/141‑1 (MQ=255)
                                                                                                                    tAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                                                                     <  2:1630142/66‑1 (MQ=255)
                                                                                                                    tAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                                                                     >  1:1630142/1‑66 (MQ=255)
                                                                                                                     aGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcagactca                         >  3:276552/1‑141 (MQ=255)
                                                                                                                       cGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGActcagactcaga                       <  2:5471/141‑1 (MQ=255)
                                                                                                                        gACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACtcagactcagac                      <  1:297258/141‑1 (MQ=255)
                                                                                                                          ttCAGATTCTGACAGCGATTCAGGCTCAGCTAGCGACTCAGACTCAGATAGTGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAg                    <  2:1293665/140‑1 (MQ=17)
                                                                                                                          cTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAg                    >  2:1375209/1‑141 (MQ=255)
                                                                                                                          cTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAg                    >  1:187331/1‑141 (MQ=255)
                                                                                                                                 tCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAGTGATTCa             >  1:438705/1‑141 (MQ=255)
                                                                                                                                         cGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAGTGATTCAGACAGCGa     <  2:1284078/141‑1 (MQ=255)
                                                                                                                                            ttCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAGTGATTCAGACAGCGATTc  >  3:608017/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGACTCAGACTCAGACAGTGATTCAGACAGCGATTC  >  USA300TCH1516_ALE/622429‑622709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑