| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,372,419 | C→T | 6.6% | intergenic (+170/+22) | USA300TCH1516_02262 → / ← pbuG | hypothetical protein/Guanine/hypoxanthine permease PbuG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,372,419 | 0 | C | T | 6.6% | 677.8 / 25.0 | 220 | intergenic (+170/+22) | USA300TCH1516_02262/pbuG | hypothetical protein/Guanine/hypoxanthine permease PbuG |
| Reads supporting (aligned to +/- strand): ref base C (98/106); new base T (11/4); total (110/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
Only 100 of 224 total aligned reads displayed.
ATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCAC > USA300TCH1516_ALE/2372279‑2372559 | atataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACTTTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCGTACTTCAAAGAAAAAAACGACTACCAATCATTGGc < 2:876902/141‑1 (MQ=255) ataGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg < 4:53712/141‑1 (MQ=255) agaagaAACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc > 2:1021050/1‑141 (MQ=255) gaagaaACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCg < 2:676943/141‑1 (MQ=255) gaagaaACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCg < 2:347357/141‑1 (MQ=255) aagaaACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGt < 1:785495/141‑1 (MQ=255) gaaACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGttt > 1:1645223/1‑141 (MQ=255) cacGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGAGG‑TTTAAAGACAGTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAATCGGCTACCAGTCATTGGCAGGCGTTTTTtg > 2:1402029/1‑141 (MQ=255) aacttCCAATAATTATAAAAGAAGTATATTTAGAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAANAAACGACTACCAATCNTTGGCAGTCGTTTTTtg < 1:1048509/137‑1 (MQ=255) acGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTtgt > 1:1417200/1‑141 (MQ=255) acGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTtgt > 2:1338081/1‑141 (MQ=255) gTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TATAAAGACATTCATAATATC‑GCAATATGATAATACGCTTCTTATTTTAACCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGAAGTCGTTTTTTgtgt > 4:227754/1‑141 (MQ=255) ccAACAATTTTTAAAGAAGTTTATTT‑TATACGGATATTTTAAAGAC‑TTTATAAATTCGGTTTTATTTTTTTCCGTTCTTTTTTTTTTCTTTATTTAAAAAAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTc < 1:218557/141‑1 (MQ=255) ccAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCt < 1:187340/141‑1 (MQ=255) aacaaTTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGACAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTgg < 1:1426663/141‑1 (MQ=255) aaTTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGAtt < 1:1350778/141‑1 (MQ=255) tAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAAcc < 1:1087226/141‑1 (MQ=255) aaaaGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCa > 2:1086177/1‑141 (MQ=255) aaaGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCAt > 3:573875/1‑141 (MQ=255) aagaagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATg < 2:188213/141‑1 (MQ=255) aagaagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATg < 1:1547420/141‑1 (MQ=255) agaagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGa > 2:1234657/1‑141 (MQ=255) gaagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATgca < 2:431531/141‑3 (MQ=255) aagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAAc < 2:913816/141‑1 (MQ=255) aagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAAc < 1:447690/141‑1 (MQ=255) aagTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GATTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAAc < 3:330193/141‑1 (MQ=255) aagTATATTTATAT‑CGCATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCGTACTTCTAAGAAAAAGACGACTATCAATCATTGGCAGTCGTTTTTTGTTTCTGGATTTAACCATGAAc < 4:418390/141‑1 (MQ=255) agTATATTTATAT‑CGGATA‑ATTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACa < 1:929299/141‑1 (MQ=255) tataTTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACaaa > 1:435869/1‑141 (MQ=255) atatTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAat < 3:487749/141‑1 (MQ=255) atatTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAat < 4:131128/141‑1 (MQ=255) tataT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGaa > 1:158411/1‑141 (MQ=255) at‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa < 2:1482618/141‑1 (MQ=255) t‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTAc < 1:370439/141‑1 (MQ=255) ta‑tTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaat < 4:246236/141‑1 (MQ=255) a‑tTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatg < 1:1298975/141‑1 (MQ=255) aaaGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATa > 2:1549955/1‑141 (MQ=255) aGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt > 1:627576/1‑141 (MQ=255) gACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTa > 1:672851/1‑141 (MQ=255) cATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTAcc > 2:280315/1‑141 (MQ=255) aTTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa > 1:1361040/1‑141 (MQ=255) aTTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa > 3:632253/1‑141 (MQ=255) tCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAtt < 4:653699/141‑1 (MQ=255) tCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAtt < 2:699233/141‑1 (MQ=255) ataataTC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTaa > 3:420115/1‑141 (MQ=255) c‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac > 1:539154/1‑141 (MQ=255) c‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac > 3:555452/1‑141 (MQ=255) ttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTa > 2:1163303/1‑141 (MQ=255) tATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTAt < 1:442678/141‑1 (MQ=255) aTGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATc < 1:495205/141‑1 (MQ=255) tGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCg < 2:742354/141‑1 (MQ=255) tATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGAt < 2:1505263/141‑1 (MQ=255) tATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGAt < 2:573301/141‑1 (MQ=255) tATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGAt < 1:1618564/141‑1 (MQ=255) tcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtctt < 1:89873/141‑1 (MQ=255) tcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTActtctt < 2:1213097/141‑1 (MQ=255) ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa < 1:1258549/141‑1 (MQ=255) ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa < 1:511501/141‑1 (MQ=255) tATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACCACCAGCATGGACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTAt > 1:1605965/1‑141 (MQ=255) tATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTAt > 4:609471/1‑141 (MQ=255) tATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTAt < 2:400367/141‑1 (MQ=255) aTTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATg < 3:60108/141‑1 (MQ=255) aTTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATg < 4:381796/141‑1 (MQ=255) tttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaat > 3:212462/1‑94 (MQ=255) tATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGtttt > 3:679688/1‑141 (MQ=255) aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttttt < 3:644334/141‑1 (MQ=255) aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGttttt < 1:423762/141‑1 (MQ=255) tCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATATAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGAAGTACTTCTTTATGtttttt > 2:1180735/1‑141 (MQ=255) aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCCGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGGTTTTTAgtga > 2:616368/1‑141 (MQ=255) ttCAAAGAAAAACACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTGACCATGAAAAAAAATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATa < 1:1402029/141‑1 (MQ=255) tCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATaa > 1:1530562/1‑141 (MQ=255) aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAGTATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc < 2:1422776/141‑1 (MQ=255) aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc < 2:973433/141‑1 (MQ=255) aaaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGc < 3:2606/141‑1 (MQ=255) aaGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGtttt < 2:1418308/128‑1 (MQ=255) aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt > 2:470282/1‑141 (MQ=255) aGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAt < 1:284640/141‑1 (MQ=255) aaaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGt < 4:555452/141‑1 (MQ=255) aaaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGt < 3:396976/141‑1 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa > 1:1503852/1‑141 (MQ=255) aaaaaCGACTACCAATCATTGACAGACGTTTTTTGTGTCTAGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAACATAGTa > 4:213150/1‑141 (MQ=255) aaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTaa > 2:1135375/1‑141 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTTGATTTAATTATTAATAAGTATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg < 2:1317818/141‑1 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg > 1:650877/1‑141 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg > 1:703324/1‑141 (MQ=255) aCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTg > 1:1157125/1‑141 (MQ=255) ctgccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATa > 2:809829/4‑141 (MQ=255) ctgccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATa > 1:1460280/4‑141 (MQ=255) ctgccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATa > 1:1016216/4‑141 (MQ=255) ctgccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATa > 1:666818/4‑141 (MQ=255) tgccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTa < 2:1654927/106‑1 (MQ=255) tACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAg < 4:45877/141‑1 (MQ=255) gccAATGATTGGTAGTCGTTTTTTTTTTTTTGTTTTAACCATGAACAAATATGAAGTACAGCATGAATAGTACCATTAATACATACATTCTCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTTTAATTGGATAGa > 2:1455615/2‑141 (MQ=255) gccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGa > 4:183893/2‑141 (MQ=255) aCCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGa < 2:50770/141‑1 (MQ=255) aatgaTTGGTAGTCGTTTTTTTTTTTTTTTTTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATATaaaa > 2:654179/5‑141 (MQ=255) aTCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaat < 1:106880/141‑1 (MQ=255) tCATTGGCAGTCGTTTTTTTTTTTTTTTTTTAATCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaata > 4:471067/1‑141 (MQ=255) cgttGGCGGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAGGCATTGTAATTGGATAGAaaataa < 1:272830/139‑1 (MQ=255) ggCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATcc > 1:1517757/1‑141 (MQ=255) | ATATAGAAGAAACACGTCCAACAATTTTAAAAGAAGTATATTTATAT‑CGGATA‑TTTAAAGACATTCATAATATC‑GCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCAC > USA300TCH1516_ALE/2372279‑2372559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |