Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,448,493 C→T 8.8% intergenic (‑252/+25) yxeP_4 ← / ← hutI putative hydrolase YxeP/Imidazolonepropionase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,448,4930CT 8.8% 681.2 / 43.0 228intergenic (‑252/+25)yxeP_4/hutIputative hydrolase YxeP/Imidazolonepropionase
Reads supporting (aligned to +/- strand):  ref base C (113/93);  new base T (12/8);  total (125/103)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.58e-01

Only 100 of 230 total aligned reads displayed.

TGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCAA  >  USA300TCH1516_ALE/2448354‑2448632
                                                                                                                                            |                                                                                                                                           
tGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCa                                                                                                                                            >  1:239714/1‑141 (MQ=255)
   tAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATg                                                                                                                                         <  1:9759/141‑1 (MQ=255)
    aGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGc                                                                                                                                        >  1:295722/1‑141 (MQ=255)
    aGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGc                                                                                                                                        >  1:897321/1‑141 (MQ=255)
     gACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATGCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCt                                                                                                                                       >  1:490781/1‑141 (MQ=255)
     gACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCt                                                                                                                                       >  1:376490/1‑141 (MQ=255)
      aCAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTACACCAATGCtt                                                                                                                                      <  2:1584192/141‑1 (MQ=255)
      aCAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCtt                                                                                                                                      <  2:1270021/141‑1 (MQ=255)
        aGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTag                                                                                                                                    <  4:179891/141‑1 (MQ=255)
          ttgttTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAGCATTGGTTTAAACTAATGTTTagag                                                                                                                                  <  2:683680/141‑1 (MQ=39)
          ttgttTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTagag                                                                                                                                  <  1:182151/141‑1 (MQ=255)
             tttAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCtt                                                                                                                               <  2:64802/141‑1 (MQ=255)
              ttAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCttt                                                                                                                              >  1:1656236/1‑141 (MQ=255)
              ttAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCttt                                                                                                                              <  1:1289360/141‑1 (MQ=255)
               taataaTATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAGCATTGGTTTAAACTAATGTTTagag                                                                                                                                  >  3:449478/1‑136 (MQ=39)
               taataaTATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAGCATTGGTTTAAACTAATGTTTagag                                                                                                                                  <  4:449478/136‑1 (MQ=39)
               taataaTATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTc                                                                                                                             >  1:1492131/1‑141 (MQ=255)
               taataaTATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTc                                                                                                                             >  3:218420/1‑141 (MQ=255)
                aataatATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCt                                                                                                                            <  2:1067762/141‑1 (MQ=255)
                 ataataTNGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTa                                                                                                                           <  2:1247257/141‑1 (MQ=255)
                   aataTCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAat                                                                                                                         <  4:358969/141‑1 (MQ=255)
                      atCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTATCTAattat                                                                                                                      >  4:383500/1‑141 (MQ=255)
                           aaCACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTAt                                                                                                                 <  1:718903/141‑1 (MQ=255)
                            acacTGTTGTCAAGCTATTCTAACTTTTATAATCGTGACTCTATACAAAAACGTGTTCTCGTATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATg                                                                                                                <  2:429172/141‑1 (MQ=255)
                             cacTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGc                                                                                                               <  2:1038959/141‑1 (MQ=255)
                                tgttgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCttt                                                                                                            >  3:615925/1‑141 (MQ=255)
                                  ttgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACGACAAAAAGCTCTAAACATTAGGTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaa                                                                                                          >  1:156140/1‑141 (MQ=255)
                                  ttgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaa                                                                                                          >  2:21951/1‑141 (MQ=255)
                                   tgtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaaa                                                                                                         <  1:775684/141‑1 (MQ=255)
                                    gtCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTaaaa                                                                                                        <  2:1084860/141‑1 (MQ=255)
                                       aaaCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGAt                                                                                                     <  2:616474/141‑1 (MQ=255)
                                       aaaCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGAt                                                                                                     >  2:782616/1‑141 (MQ=255)
                                         aCTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATAc                                                                                                   >  2:899100/1‑141 (MQ=255)
                                             ttCTAACTTTTATAATTGAGACTCTATACAAAAACGTTTGCTCTAATAGACGTGGTTTTACATACCACAAAAAGCTCAAAACATCAGTTTAAACCAATTCTTAGAGCTTACAAATTACAGTATGCTTTAAAAGNTACtcga                                                                                               >  2:1242264/1‑138 (MQ=255)
                                             ttCTAACTTTTATAATTGAGACCCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTACTTATTTTCTGCTTTACAAGATACtgtg                                                                                               >  1:1361453/1‑141 (MQ=255)
                                              tCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTgtgt                                                                                              >  3:53326/1‑141 (MQ=255)
                                              tCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTgtgt                                                                                              >  1:1418053/1‑141 (MQ=255)
                                               cTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGtt                                                                                             <  1:586761/141‑1 (MQ=255)
                                                 aaCTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTAt                                                                                           <  4:554406/141‑1 (MQ=255)
                                                  aCTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATc                                                                                          <  3:212529/141‑1 (MQ=255)
                                                  aCTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATc                                                                                          <  3:30180/141‑1 (MQ=255)
                                                  aCTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCATTAAAAGATACTGTGTTATc                                                                                          >  3:87975/1‑141 (MQ=255)
                                                    ttttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTa                                                                                        >  1:1338168/1‑141 (MQ=255)
                                                      ttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTCCAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACg                                                                                      >  2:162643/1‑141 (MQ=255)
                                                      ttATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACg                                                                                      <  2:467829/141‑1 (MQ=255)
                                                       tataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTCCGTTATACTGTGTTATCTACGa                                                                                     >  1:851102/1‑141 (MQ=255)
                                                       tataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGa                                                                                     >  3:498639/1‑141 (MQ=255)
                                                        ataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAACGATCTAAACCTTCGTTTACACAACTGCTTAGAGATTTCTAATTATTTTATGCTTTAACAGATACTGTGTTATCTACGAt                                                                                    <  1:1074582/141‑1 (MQ=255)
                                                        ataATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGAt                                                                                    <  2:1369274/141‑1 (MQ=255)
                                                         taATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATg                                                                                   >  1:418421/1‑141 (MQ=255)
                                                         taATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATg                                                                                   >  4:110875/1‑141 (MQ=255)
                                                          aaTTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGa                                                                                  >  3:236505/1‑141 (MQ=255)
                                                           aTTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGAc                                                                                 >  1:1480182/1‑141 (MQ=255)
                                                             tGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCt                                                                               >  3:35275/1‑141 (MQ=255)
                                                                 aCTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTAc                                                                            >  2:951042/1‑141 (MQ=255)
                                                                 aCTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTAcc                                                                           >  1:16926/1‑141 (MQ=255)
                                                                 aCTCTATAAAGAAAAGTATTTTGAAATATACTTGTTTTTACAAACCACAAACATCTCTAAACATTATTTTAAACCAATGCTTAGAGGTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGAttaccttacc                                                                           <  4:646945/141‑1 (MQ=255)
                                                                   tctATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGt                                                                         >  4:625782/1‑141 (MQ=255)
                                                                       taCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTc                                                                                                                             >  2:1631245/1‑85 (MQ=255)
                                                                       taCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCttt                                                                     >  1:562254/1‑141 (MQ=255)
                                                                       taCAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCttt                                                                     >  1:113543/1‑141 (MQ=255)
                                                                          aaaaaCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaat                                                                  <  2:1492131/141‑1 (MQ=255)
                                                                          aaaaaCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaat                                                                  >  1:906761/1‑141 (MQ=255)
                                                                            aaaCGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTtaataa                                                                >  2:740694/1‑141 (MQ=255)
                                                                               cGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACtt                                                             >  3:465127/1‑141 (MQ=255)
                                                                                gtgtTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACttt                                                            >  2:249879/1‑141 (MQ=255)
                                                                                 tgtTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACtttt                                                           >  3:607555/1‑141 (MQ=255)
                                                                                         aaTATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACATTCCCGTCTTTAATAACTTTTTCTGCGtg                                                   >  2:1167589/1‑141 (MQ=255)
                                                                                             taCTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGtgattg                                               <  1:643440/141‑1 (MQ=255)
                                                                                                 tGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATAc                                           <  1:764569/141‑1 (MQ=255)
                                                                                                  gTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATAcc                                          <  2:790219/141‑1 (MQ=255)
                                                                                                     ttttACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGCGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCaa                                        <  4:694391/141‑1 (MQ=255)
                                                                                                      tttACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCaaa                                       <  4:615925/141‑1 (MQ=255)
                                                                                                         aCAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    >  1:1212872/1‑141 (MQ=255)
                                                                                                          cAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGa                                   >  3:694070/1‑141 (MQ=255)
                                                                                                              ccACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATg                               <  2:376490/141‑1 (MQ=255)
                                                                                                              ccACAAAAAGCTCTAAACATTAGTCTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATg                               <  2:130579/141‑1 (MQ=255)
                                                                                                                acaAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGa                             >  1:28196/1‑141 (MQ=255)
                                                                                                                    aaaGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAatat                         >  4:232854/1‑141 (MQ=255)
                                                                                                                     aaGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAatata                        <  4:486601/141‑1 (MQ=255)
                                                                                                                      aGCTCTAAGCATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATACGCAatatat                       >  2:503175/1‑141 (MQ=38)
                                                                                                                        ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:1273052/141‑1 (MQ=255)
                                                                                                                        ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:1174207/141‑1 (MQ=255)
                                                                                                                        ctctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:432154/141‑1 (MQ=255)
                                                                                                                        ctctAAACATTAGTTGAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTc                     <  1:1374896/141‑1 (MQ=255)
                                                                                                                         tctAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    >  1:1625074/1‑141 (MQ=255)
                                                                                                                           taagcATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATg                  >  1:1024766/5‑141 (MQ=255)
                                                                                                                             agcATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    <  2:845786/119‑1 (MQ=39)
                                                                                                                             agcATTGGTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATg                                    >  1:845786/3‑121 (MQ=39)
                                                                                                                             aaCATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGAt                >  1:1579153/1‑141 (MQ=255)
                                                                                                                                 ttggTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTgg            >  1:627966/4‑141 (MQ=255)
                                                                                                                                 ttggTTTAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCAGGATTTgg            >  3:639816/4‑141 (MQ=255)
                                                                                                                                  tAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGt           >  4:47495/1‑141 (MQ=255)
                                                                                                                                    gTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGc         >  3:169930/1‑141 (MQ=255)
                                                                                                                                      ttAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    <  1:1447761/128‑1 (MQ=255)
                                                                                                                                      ttAAACTAATGTTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCa                    >  2:1447761/1‑128 (MQ=255)
                                                                                                                                      ttAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCAt       <  3:563305/141‑1 (MQ=255)
                                                                                                                                         aaCCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTgtcaata    >  4:512727/1‑135 (MQ=255)
                                                                                                                                          aCCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCa   >  1:869574/1‑141 (MQ=255)
                                                                                                                                           ccAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCaa  <  2:865487/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TGATAGACAGTTGTTTAATAATATCGTAACACTGTTGTCAAACTATTCTAACTTTTATAATTGAGACTCTATACAAAAACGTGTTCTCGAATATACTTGTTTTTACAAACCACAAAAAGCTCTAAACATTAGTTTAAACCAATGCTTAGAGCTTTCTAATTATTTTATGCTTTAAAAGATACTGTGTTATCTACGATGACCTTACCGTCTTTAATAACTTTTTCTGCGTGATTGATACCAAAATGATATGGAATATATTCATGATTTGGTGCATCCCAA  >  USA300TCH1516_ALE/2448354‑2448632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: