BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,846	A→G	5.5%	S780S (AGT→AGC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,846	0	A	G	5.5%	536.7 / 7.2	196	S780S (AGT→AGC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (86/96); new base G (9/4); total (96/100)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGA  >  USA300TCH1516_ALE/2774709‑2774986

gAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTg                                                                                                                                           <  2:626242/141‑1 (MQ=255)

gAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTg                                                                                                                                           <  2:464079/141‑1 (MQ=255)

    cGGAGTCATTATCTGAGTTTGATTCGGTATCTGAATCCGAGTTGCTATCCGAGTCTGAGTAGCTCTCTGAGTCTGAATCGCAGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTATg                                                                                                                                       <  2:1022975/141‑1 (MQ=17)

    cGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTg                                                                                                                                       <  2:378826/141‑1 (MQ=255)

         tCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATcc                                                                                                                                  >  2:939250/1‑141 (MQ=255)

                  gagtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                                                                                                                         >  1:684853/1‑141 (MQ=255)

                    gtctgagtcGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTatc                                                                                                                       >  1:723133/1‑141 (MQ=255)

                    gtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatc                                                                                                                       <  3:597115/141‑1 (MQ=255)

                      ctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctg                                                                                                                     >  4:573579/1‑141 (MQ=255)

                      ctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctg                                                                                                                     >  3:440617/1‑141 (MQ=255)

                       tgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTAGATGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctga                                                                                                                    <  3:158042/141‑1 (MQ=255)

                        gagtcGCTGTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                                   <  4:109453/141‑1 (MQ=255)

                        gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                                   >  3:369894/1‑141 (MQ=255)

                          gtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatc                                                                                                                 >  1:1233504/1‑141 (MQ=255)

                           tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatct                                                                                                                <  1:158920/141‑1 (MQ=255)

                            cGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctg                                                                                                               <  1:696904/141‑1 (MQ=255)

                              cTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTAtctgaatctgaa                                                                                                             >  2:807267/1‑141 (MQ=255)

                                aTCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCtgaatctgaatc                                                                                                           <  1:1322659/141‑1 (MQ=255)

                                 tCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGCGTCTGAGTCGCTGTCTGAGTCTGAATCTCTGTCTGAATCTGAATCGCTATCTGAATCTGAATCg                                                                                                          >  2:794727/1‑141 (MQ=255)

                                 tCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCg                                                                                                          >  2:804727/1‑141 (MQ=255)

                                  cTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                         <  2:727929/141‑1 (MQ=255)

                                       tCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATcc                                                                                                    <  1:1333549/141‑1 (MQ=255)

                                        ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                   <  1:2292/141‑1 (MQ=255)

                                        ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                   >  2:1504096/1‑141 (MQ=255)

                                          gAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGCGTATATGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAg                                                                                                 <  1:524656/141‑1 (MQ=255)

                                          gAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAg                                                                                                 >  1:441342/1‑141 (MQ=255)

                                            gTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTc                                                                                               <  1:31541/141‑1 (MQ=255)

                                             tCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCt                                                                                              >  4:42367/1‑141 (MQ=255)

                                              cGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGa                                                                                                                                      <  1:110487/100‑1 (MQ=255)

                                                cTATCCGGGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                           <  4:258419/141‑1 (MQ=255)

                                                cTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                           >  2:1388232/1‑141 (MQ=255)

                                                   tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGGTGTCCGAGTCCGACTCGATATCTGAATCTGGGTCGTTGGCCTAGTCTGaaacg                                                                                        >  4:333313/1‑137 (MQ=11)

                                                   tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCg                                                                                        >  2:240622/1‑141 (MQ=14)

                                                    ctgAGGCTGCGTCGCTCTCCGAGTCCGACTCTCTATCTGAGTCTCTATCCCTATCCGACTCTGTTTCGCTCTCTGAGTCTGAATCACTGTCTGAATCCGACTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGc                                                                                       <  2:499468/139‑1 (MQ=2)

                                                    ccGTGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                       <  2:417638/141‑1 (MQ=255)

                                                    ccGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                       <  3:123303/141‑1 (MQ=255)

                                                    ccGAGTCTGAGTCGCTATCTGAGTCGGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                       <  2:75520/141‑1 (MQ=255)

                                                      gAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                                                                     <  1:1125494/141‑1 (MQ=255)

                                                      gAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                                                                     >  1:437846/1‑141 (MQ=255)

                                                        gTCTGGGTCGCTATCTGAGTCGGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtc                                                                                   <  2:1148199/141‑1 (MQ=17)

                                                            gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAGTCTGAATCGCTGTCCGAGTCTGAGTCGCTGtctgag                                                                               >  2:1623695/1‑140 (MQ=17)

                                                             ggTCGCTTTTTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaat                                                                              <  2:1085849/140‑1 (MQ=14)

                                                             aGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaat                                                                              >  1:63813/1‑141 (MQ=255)

                                                               tCGCTATCTGAGTCTGAATCGCTGTCTGACTCTGCGCCGCTATCCGAGTCAGAGTCGCTCTCTGACTCTGAATCACCGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCCATCCGAGTCCGAATCGCTGtaagaatca                                                                            >  2:151528/1‑140 (MQ=17)

                                                                  cTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                                                         <  1:362632/141‑1 (MQ=255)

                                                                  cTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                                                         <  4:356143/141‑1 (MQ=255)

                                                                     tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTa                                                                                                       >  3:492704/1‑108 (MQ=255)

                                                                     tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTa                                                                                                       <  4:492704/108‑1 (MQ=255)

                                                                          gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                       <  2:421533/119‑1 (MQ=255)

                                                                          gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                       >  1:421533/1‑119 (MQ=255)

                                                                          gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTc                                                                 >  2:914355/1‑141 (MQ=255)

                                                                           tCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCt                                                                <  1:553828/141‑1 (MQ=255)

                                                                              gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTATCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                                                             <  4:186103/141‑1 (MQ=255)

                                                                                aTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCGGAGTc                                                           >  4:273143/1‑141 (MQ=255)

                                                                                aTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCAGAGTc                                                           >  2:48692/1‑141 (MQ=255)

                                                                                 tCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTGTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTca                                                          >  2:176323/1‑140 (MQ=255)

                                                                                   gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGGATCGGAATCGCTTTCCGAGTCTGACTCGCCGTCTGACTCTGAATCGCTGTCTGAGTACGa                                                        >  2:677066/1‑141 (MQ=17)

                                                                                    cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                    >  1:1188645/1‑128 (MQ=255)

                                                                                    cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCt                                                                    <  2:1188645/128‑1 (MQ=255)

                                                                                    cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                       <  1:203432/141‑1 (MQ=255)

                                                                                    cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                       <  1:845258/141‑1 (MQ=255)

                                                                                    cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                       <  3:247614/141‑1 (MQ=255)

                                                                                       tctgagtctgagTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                           <  3:676341/102‑1 (MQ=255)

                                                                                             tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAAACTGAATCGCCGTCTGAGTCCGAAGCGCTatcg                                              >  2:20854/1‑140 (MQ=255)

                                                                                             tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatct                                              >  3:35059/1‑141 (MQ=255)

                                                                                                gagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaa                                           <  1:1150279/141‑1 (MQ=255)

                                                                                                  gtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGGATCTGAATCGCTGTCTGAg                                                             >  1:1223372/1‑121 (MQ=255)

                                                                                                  gtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                                                             <  2:1223372/121‑1 (MQ=255)

                                                                                                  gtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatc                                         <  1:1504096/141‑1 (MQ=255)

                                                                                                  gtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga                                            >  2:1255976/1‑138 (MQ=255)

                                                                                                      cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                     <  1:1170766/141‑1 (MQ=255)

                                                                                                      cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                     >  3:199953/1‑141 (MQ=255)

                                                                                                            gtatCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGATTCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                               <  2:288174/138‑1 (MQ=255)

                                                                                                            gAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                               <  3:463179/141‑1 (MQ=255)

                                                                                                               tCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGATTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                            <  1:865933/141‑1 (MQ=255)

                                                                                                               tCTGAGTCGCTGTCGGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                            <  1:520801/141‑1 (MQ=255)

                                                                                                                cTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGACTCTGAATCGCTGTCTGCGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctg                           >  1:751054/1‑141 (MQ=255)

                                                                                                                 tGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctga                          <  1:914355/141‑1 (MQ=255)

                                                                                                                  gAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAAGCGCGATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGATTCTGAATCGCTGTCTGAGTCCGAGTCGCTGTCTGAATCTGAGTCGCTctctgag                         >  2:1138174/1‑141 (MQ=11)

                                                                                                                       gCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctga                    <  4:392995/141‑1 (MQ=255)

                                                                                                                           tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCg                <  2:1462442/141‑1 (MQ=255)

                                                                                                                            ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc               >  4:692168/1‑141 (MQ=255)

                                                                                                                             tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                     <  4:670937/118‑1 (MQ=255)

                                                                                                                              gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa             <  1:1412174/141‑1 (MQ=255)

                                                                                                                              gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa             <  4:241052/141‑1 (MQ=255)

                                                                                                                              gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa             <  4:579277/141‑1 (MQ=255)

                                                                                                                                atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc           >  1:1377921/1‑141 (MQ=255)

                                                                                                                                atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTATCTGAGTCCGAATCGCTATCTGAATCTGAGTGGCTGTCCGACGCTGAGTCGCtgtg           >  2:841208/1‑138 (MQ=17)

                                                                                                                                 tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatct          >  1:182783/1‑141 (MQ=255)

                                                                                                                                 tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatct          <  2:94108/141‑1 (MQ=255)

                                                                                                                                  ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                         <  3:398031/93‑1 (MQ=255)

                                                                                                                                  ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTgtctg         >  2:1468079/1‑141 (MQ=255)

                                                                                                                                    gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa       >  2:14271/1‑141 (MQ=255)

                                                                                                                                     aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGGATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat      <  2:1068054/141‑1 (MQ=255)

                                                                                                                                     aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCCGAGTCCGGATCGCTATCTGAATCTGAGTCGCTGTCTGTGTCTGAATCTCTatctgaat      <  2:569788/141‑1 (MQ=255)

                                                                                                                                      atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGATTCGCTGTCCACGACCGAGTCGCTCTCTCACTAGGAGTGCCTGTCTGAGCCCGAATCGCTatgtgaaac     >  1:499468/1‑139 (MQ=17)

                                                                                                                                      atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc     >  2:821291/1‑141 (MQ=255)

                                                                                                                                       tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                           <  1:1635578/54‑1 (MQ=255)

                                                                                                                                        cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg   >  1:567303/1‑141 (MQ=255)

                                                                                                                                         aCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga  >  1:284709/1‑141 (MQ=255)

GAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGA  >  USA300TCH1516_ALE/2774709‑2774986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`