Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,513,930 A→T 100% L413F (TTA→TTT)  USA300HOU_RS07375 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,513,9300AT100.0% 297.1 / ‑6.5 105L413F (TTA→TTT) USA300HOU_RS07375hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (52/52);  total (52/53)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 105 total aligned reads displayed.

TTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGA  >  USA300TCH1516_ALE/1513800‑1514057
                                                                                                                                  |                                                                                                                               
ttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATAACTCATAATATATTTTAtt                                                                                                                              >  1:1712476/1‑134 (MQ=255)
ttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTAACAAATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTAtt                                                                                                                              <  2:1428057/134‑1 (MQ=255)
    atgtatCGCTTTATTAGTAGCCATTGGCATGAACACTCTTTCCATGATAAAGAGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAt                                                                                                                          <  2:1292862/134‑1 (MQ=255)
     tgtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                         >  1:1817529/1‑134 (MQ=255)
     tgtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATATATATCACTCATAATATGTTTTATTGTAtt                                                                                                                         >  2:1688318/1‑134 (MQ=255)
     tgtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTAACACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                         <  2:1031791/134‑1 (MQ=255)
     tggatCTCTTTATTAGTAGGCATTGGGATGGGGTTTGTCTGTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                         <  2:1590783/131‑1 (MQ=255)
       tatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAg                                                                                                                       >  2:722537/1‑134 (MQ=255)
        atCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAgg                                                                                                                      <  1:2578828/134‑1 (MQ=255)
        atCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACAAGCATCTATATCAATCATCATATATTTTATTGTATTact                                                                                                                      >  2:2624449/1‑132 (MQ=255)
          cGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGat                                                                                                                    >  2:1685835/1‑134 (MQ=255)
            cTTTATTAGTAGTCATTGGCATGGGGTTTGTCTTTCTGTTAAATCTTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGatta                                                                                                                  <  1:2704566/134‑1 (MQ=255)
            cTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGatta                                                                                                                  <  1:169688/134‑1 (MQ=255)
              ttattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGattatt                                                                                                                >  1:1309290/1‑134 (MQ=255)
              ttattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATAACTCATAATATATTTTATTATATTAGAattatt                                                                                                                >  2:1828119/1‑134 (MQ=255)
               tattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATTTTTTATTGTATTAGGAttatta                                                                                                               >  1:161396/1‑134 (MQ=255)
                attaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAGTAGGATTATTaa                                                                                                              >  1:1605576/1‑134 (MQ=255)
                 ttaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAGATACTGCAATAAAAGTAACACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAAt                                                                                                             >  1:1411972/1‑134 (MQ=255)
                 ttaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAAt                                                                                                             >  1:1050948/1‑134 (MQ=255)
                  tagtagCCAGTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAGAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATg                                                                                                            <  2:2360879/134‑1 (MQ=255)
                    gtagCCATTGGCATGGGGTTTGTCCCTTGGATAACGCGTACAAAAAATACTACAATAAAAGTACCACATCGCGTCAAAAAACCAGCATCTATCACCCCCATAATATATCTTATTGTATTAGGAATCTTAATGcc                                                                                                          >  2:1266684/1‑134 (MQ=255)
                     tagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCTCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCa                                                                                                         <  1:263032/134‑1 (MQ=255)
                     tagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCa                                                                                                         >  2:2117001/1‑134 (MQ=255)
                      agCCATTGGCATGGTGTTTTTCTCTTTGATAAAGCGTACAAAAAATACTGCAATAAAAGTTCCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCat                                                                                                        <  2:2698527/134‑1 (MQ=255)
                      agCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCat                                                                                                        >  1:1049639/1‑134 (MQ=255)
                             ggCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATTGCGTAAGTGATCCCCCAGTTAGATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTgg                                                                                                 <  1:1099278/134‑1 (MQ=255)
                             ggCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCAGTATTTCTATCATTTATAATATTAGGATTATTAATGCCATTATTTgg                                                                                                 >  1:1223428/1‑134 (MQ=255)
                             ggCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTgg                                                                                                 >  1:8942/1‑134 (MQ=255)
                               cattgcgTTTCTCTCTTGGAAAAAGCGGACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGAt                                                                                               <  2:1297096/128‑1 (MQ=255)
                                aTGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTTCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGAtt                                                                                              <  1:2531862/134‑1 (MQ=255)
                                aTGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACCTAGCGTAAGTAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGTTTATTAATGCCATTATTTGGAtt                                                                                              >  1:1098209/1‑134 (MQ=255)
                                aTGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATTCCATTATTTGGAtt                                                                                              >  1:2452982/1‑134 (MQ=255)
                                aTGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGAtt                                                                                              >  2:1359188/1‑134 (MQ=255)
                                aTGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAAACAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGAtt                                                                                              >  1:57988/1‑134 (MQ=255)
                                 tGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTa                                                                                             <  1:965565/134‑1 (MQ=255)
                                  ggggTTTGTTTCTTGGATAAAGTGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCAGCGATATCGCTAATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTAt                                                                                            <  1:909832/134‑1 (MQ=255)
                                   gggTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATTCCATTATTTGGATTAta                                                                                           >  2:1647747/1‑133 (MQ=255)
                                    ggTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCTCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCa                                                                                          <  1:1387460/134‑1 (MQ=255)
                                      tttGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACt                                                                                        <  1:1200010/134‑1 (MQ=255)
                                       ttGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATACAAGTACCACATCGCGTAAAAAAACCAGCATCTATATAAATAAAAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCAttt                                                                                       >  1:40389/1‑134 (MQ=255)
                                       ttGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACtt                                                                                       >  1:1828585/1‑134 (MQ=255)
                                        tGTCTCTTGTATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTa                                                                                      <  1:4187/134‑1 (MQ=255)
                                         gTCGCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGGTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTAt                                                                                     <  2:1470752/134‑1 (MQ=255)
                                           gtattagATAAAGCGAATGAAAAAAACTACTATAAAAGTACCAAATAGCATACAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATcc                                                                                   <  2:2627679/128‑1 (MQ=255)
                                           ctctTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATcc                                                                                   >  2:2334855/1‑134 (MQ=255)
                                            tctTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCt                                                                                  <  2:1309290/134‑1 (MQ=255)
                                             ctTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACTACATCGCGTAAAAAAACCAACATCTATATCACTCATAATATGTTTTATTGTATTACTATTATTAATGCCATTATTTGGATTATCACTTaccttt                                                                                 >  2:310797/1‑129 (MQ=255)
                                                  aTAAAGAGTAGAAGAGATGATGAAAGAAAAGTACCACGTCGCGTAAAAAAACCAACATCTATATCACTCGTAATGTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTAtt                                                                            <  2:2151336/134‑1 (MQ=255)
                                                     aaGCGTACAAAAAATATTGCAATAAAAGTACCACATCGCGTAAAAAAACCACCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTtat                                                                         <  2:1546912/134‑1 (MQ=255)
                                                     aaGCGTACAAAAAATACTGCAATATAAGTACCACATCGCGTAAAAAAAACAGCATTTATATCACTAATAATATGTTTTATTGTATTGGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTtat                                                                         >  1:53003/1‑134 (MQ=255)
                                                     aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCCCTCATAATATGCTTTATTGTATTAGGATTATTAATTCCATTATTTGTATTATCACTTACTCTTCTATTtat                                                                         >  2:2787431/1‑134 (MQ=255)
                                                     aaGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATAAGGATTATTAATACCATTATTTGGATTATCACTTATCCTTGTATTtat                                                                         >  1:871383/1‑134 (MQ=255)
                                                     aaGCGTAAAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTtat                                                                         <  2:2497167/134‑1 (MQ=255)
                                                       gCGTACAAAAAATACTTCAATAAAAGAACCACATCGCTTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTATGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                       <  2:143170/134‑1 (MQ=255)
                                                       gCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAATCAGCATCTATTTCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                       <  1:1324670/134‑1 (MQ=255)
                                                       gCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAAGATTATTAATGACATTATTTGGATTATCACTTATCCTTGTATTTATaa                                                                       >  1:12991/1‑134 (MQ=255)
                                                        cGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAAt                                                                      <  2:1756070/134‑1 (MQ=255)
                                                        cGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAAt                                                                      <  1:2446667/134‑1 (MQ=255)
                                                         gTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGCTTATTAATACCATTATTTGGATTATCACTTATCCTTGTATTTCTAAtt                                                                     >  2:771449/1‑134 (MQ=255)
                                                           actacaaaTACTGAAATAAAAGTACAACGTCGCGTAAAATATCCAGCATCTATATCACTCATAATATTTTTTATTGTATTAGGCTTATTAATGCCATTATTTTGATTATCACTTATCCTTGTATTTATAATTGa                                                                   <  2:232849/129‑1 (MQ=255)
                                                           aCAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCATTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGa                                                                   <  1:722537/134‑1 (MQ=255)
                                                            cAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGTATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                  <  1:592423/134‑1 (MQ=255)
                                                            cAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                  <  1:2590092/134‑1 (MQ=255)
                                                             aaaaaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCATCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAAt                                                                 <  1:2424982/134‑1 (MQ=255)
                                                              aaaaaTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCATTTTTAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAAtt                                                                <  1:46902/134‑1 (MQ=255)
                                                               aaaaTACTGCAATAAAAGTATCACATCGCGTAAAAAAACCAGCATCTATATCACTTATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTa                                                               <  1:1758070/134‑1 (MQ=255)
                                                                  aTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGACATTATTTGGATTATCACTTATACTTGTATTTATAATTGACTTAata                                                            >  2:1963154/1‑134 (MQ=255)
                                                                         aaTAAAAGTACCACATCGCGTAAAAAACTCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATtatata                                                     <  1:719751/134‑1 (MQ=255)
                                                                         aaTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATtatata                                                     <  1:739194/134‑1 (MQ=255)
                                                                            aaaaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTa                                                  <  2:728009/134‑1 (MQ=255)
                                                                             aaaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTaa                                                 <  2:1178939/134‑1 (MQ=255)
                                                                              aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCCTAATATGTTTTATTCTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTaaa                                                >  2:278062/1‑134 (MQ=255)
                                                                              aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTTTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTaaa                                                <  1:126216/134‑1 (MQ=255)
                                                                              aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTaaa                                                >  2:1481656/1‑134 (MQ=255)
                                                                              aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCATTGTATTTATAATTGAATTAATATTATATATTaaa                                                >  2:1013266/1‑134 (MQ=255)
                                                                               aGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAg                                               >  1:2608131/1‑134 (MQ=255)
                                                                               aGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATACCATTATTTGGATTATCACTTATCCTTGTATTTATAATTTAATTAATATTATATATTAAAg                                               >  1:242977/1‑134 (MQ=255)
                                                                               aGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATCGTATTAGAATTATTAATTCCATTATTTAAATTAACACTTATCCATGTATTAATAATTGAATTAATAATATATATTAAAg                                               >  1:565980/1‑134 (MQ=255)
                                                                                gTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGa                                              <  1:2769199/134‑1 (MQ=255)
                                                                                atactacATCGCGTAAAAAAACCAGTATCTATTTCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGa                                              <  1:2351341/133‑1 (MQ=255)
                                                                                   ccACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGAATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAtcg                                           >  1:2768462/1‑134 (MQ=255)
                                                                                       aTCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATcgtcgt                                       <  2:1984183/134‑1 (MQ=255)
                                                                                       aTCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATcgtcgt                                       <  2:2682811/134‑1 (MQ=255)
                                                                                        tCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                      >  1:2783107/1‑134 (MQ=255)
                                                                                        tCGAGTAAAAAAACCAGCATCTATATAAATAATAATATATATTATTATATTAGAATTATTAATGCCATTATTTGGATTATCACTTATCCTTTTATTTATAATTTTATTAATATTATATATTAAAGATCGTCGTg                                      >  2:82852/1‑134 (MQ=255)
                                                                                         cgcgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGc                                     >  1:2037717/1‑134 (MQ=255)
                                                                                          gcgTAAGAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCt                                    <  2:2508746/134‑1 (MQ=255)
                                                                                          gcgTAAAAAAACCATCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCt                                    <  1:822157/134‑1 (MQ=255)
                                                                                          gcgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCt                                    <  1:2624449/134‑1 (MQ=255)
                                                                                           cgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTa                                   >  2:2493185/1‑134 (MQ=255)
                                                                                           cgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCATTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTa                                   >  2:42497/1‑134 (MQ=255)
                                                                                             tAAAAAAACCAGTATCTATATCTCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTaaa                                 <  2:553651/134‑1 (MQ=255)
                                                                                              aaaaaaaCCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGTATTATTAATGCCATTATTTGGAGTATCACTTAGCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAAc                                <  1:2800591/134‑1 (MQ=255)
                                                                                                       agtaTCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCa                       <  2:1164457/131‑1 (MQ=255)
                                                                                                          aTCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATAGTCGTGCTAACCAATAATGCACtt                    >  2:72277/1‑134 (MQ=255)
                                                                                                          aTCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCAATTATTTTTATATTTATAATTGAATTAATATTATATATTAAAGATCGTAGTGTTAAAAAATAATGCAAtt                    >  1:1774797/1‑134 (MQ=255)
                                                                                                          aTCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGAATTATCACTTATCCTTAAATTTATAATTGAATTAATATTATATATTAAAGATCTTCATGCTAAACAATAATGCACtt                    >  1:2308822/1‑134 (MQ=255)
                                                                                                               tatCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGt               >  2:1625302/1‑134 (MQ=255)
                                                                                                                 tctcTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTTGATTATCACTTATTCTTGTATTTATAATTGGATTAATATTAAATATTATAATTGATCGTTCTATACAATAATGCACTGAAAGttt             <  1:2730253/131‑1 (MQ=255)
                                                                                                                            atGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTCTTTATAATTGAATTAATATTATATCTTACATCTGAACTCGCATAAAAATAATGCACTTAAAGTGTTGAACTTACGa  >  1:2090688/1‑134 (MQ=255)
                                                                                                                                  |                                                                                                                               
TTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGA  >  USA300TCH1516_ALE/1513800‑1514057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑