BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,512	A→G	32.4%	S194S (TCA→TCG)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,512	0	A	G	32.4%	264.4 / 191.1	229	S194S (TCA→TCG)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (77/78); new base G (29/45); total (106/123)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 230 total aligned reads displayed.

GGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATA  >  USA300TCH1516_ALE/111380‑111645

agAAGTTACAGAAGAAAACATGAAAGAAGAAATCGAAAACTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                      <  2:1876431/133‑1 (MQ=255)

agAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGTAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAg                                                                                                                                      <  1:375060/133‑1 (MQ=255)

 gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGGTATATCATATAATCCAGAGGTGCCGAGTTATTCAGc                                                                                                                                     <  1:1575290/134‑1 (MQ=255)

  aaGTTAAAGAAGAAATCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                                                    <  1:1796849/134‑1 (MQ=255)

   aattAAAGAAGAAAACATAAAAAAAGAAATAGAAAAACATAAGTATATTGAGAACAATAGCAATTTTAACGATTTAAGGAATTATAAAGATGGAAATATTTTTTAAAAATTATGGTTGCCGAGTTATTCAGCTc                                                                                                                                   <  2:347061/132‑1 (MQ=255)

   aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCta                                                                                                                                   >  1:493594/1‑133 (MQ=255)

   aGTTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                   <  2:1014755/134‑1 (MQ=255)

    gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCtaa                                                                                                                                  <  2:1841314/134‑3 (MQ=255)

     ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGATCaa                                                                                                                                 >  1:172794/1‑134 (MQ=255)

      tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                <  2:686793/134‑1 (MQ=255)

      tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTCTGCGCAATATAGCAATTTTAAAGATATAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                >  1:807099/1‑134 (MQ=255)

       aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                               >  2:1180166/1‑134 (MQ=255)

       aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAAAAAAGCCGAGTTATTCAGCTCAata                                                                                                                               >  1:2699552/1‑134 (MQ=255)

        aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                              >  1:404114/1‑134 (MQ=255)

        aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                              >  2:2388232/1‑134 (MQ=255)

         agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGGAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATc                                                                                                                             <  2:1736583/134‑1 (MQ=255)

          gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                            >  1:1937577/1‑134 (MQ=255)

           aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCaa                                                                                                                           <  2:2800369/134‑1 (MQ=255)

            agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGAAGATATACAATATAATCCAAATGTTCCGAGTTATTCAGCTCAATATCAAt                                                                                                                          >  1:2230287/1‑134 (MQ=21)

             gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                                         <  1:359015/134‑1 (MQ=255)

               aaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTaa                                                                                                                       <  1:298533/134‑1 (MQ=255)

                 aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                     >  1:1553680/1‑134 (MQ=255)

                 aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCAACTCAATATCAATTAACt                                                                                                                     >  1:524502/1‑134 (MQ=255)

                 aCATAAAAAAAGAAATCGAAAACATTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                     <  2:2450669/134‑1 (MQ=255)

                  cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTa                                                                                                                    <  2:1489059/134‑1 (MQ=255)

                        aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                              <  2:2150809/134‑1 (MQ=255)

                         aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAAtgatga                                                                                                             >  2:46317/1‑134 (MQ=255)

                          aaaaaaTCGAAATCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                            <  1:2656689/131‑1 (MQ=255)

                            gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                          <  1:2810615/134‑1 (MQ=255)

                                tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                                                      >  2:1354340/1‑134 (MQ=255)

                                tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                      >  1:2412754/1‑134 (MQ=255)

                                 cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                                                     <  2:2214799/134‑1 (MQ=255)

                                 cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                                                     <  2:1088239/134‑1 (MQ=255)

                                   aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                                                   <  1:1513596/134‑1 (MQ=255)

                                   aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                                                   >  1:2297668/1‑134 (MQ=255)

                                    aaccTTTAAGTTTTTTGCGCAATATATCAATTTTAAAGATTTAATTATTTATAATTATGGTTATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  1:115295/131‑1 (MQ=17)

                                    aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  2:764870/134‑1 (MQ=255)

                                    aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  2:35812/134‑1 (MQ=255)

                                      aCTTTAAGTTTTTTGCGTAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                <  2:13999/134‑1 (MQ=255)

                                        tttAAGTTGTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                              <  2:1989077/134‑1 (MQ=255)

                                            aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGCTGCCGAATAAACAAACTGAGTATGACTTCACTAATTATGATTATAATTTAAAACAATTa                                                                                          >  2:606022/1‑134 (MQ=25)

                                             gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                         <  1:2045802/134‑1 (MQ=255)

                                              ttttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                        <  1:2282479/134‑1 (MQ=255)

                                                ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                      <  2:122138/134‑1 (MQ=255)

                                                ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                      <  1:1647427/134‑1 (MQ=255)

                                                   tgtacAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                                                   <  2:2711115/130‑1 (MQ=14)

                                                    gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                                                  >  2:170430/1‑134 (MQ=255)

                                                     cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGTAGATATATCATATAATCCAGAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                 <  1:786746/134‑1 (MQ=255)

                                                     cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATCATCACAAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTACAACACTTCCGCAAACGa                                                                                 >  2:2501094/1‑134 (MQ=17)

                                                     agcAATATAGCAATTTTAAAGATTTAATGAAGTATAAAGATTGAGATATATCATATAATTCAGAGGTGCCGAGTTATTCAGCTCAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                 <  1:758671/133‑1 (MQ=255)

                                                      gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAACAATTACGAAACAGat                                                                                >  2:1173959/1‑134 (MQ=255)

                                                         atatAGCAATTTTAAAGATTTAATGAATTGTTATAACTAAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATg                                                                             <  1:1957346/134‑1 (MQ=18)

                                                         atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATg                                                                             >  1:2612494/1‑134 (MQ=255)

                                                         atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATg                                                                             <  2:1900526/134‑1 (MQ=255)

                                                          tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATATTTCAGAGGTGCCGAGTTATTCGGATAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                            <  1:250825/134‑1 (MQ=14)

                                                          tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                            <  1:1000925/134‑1 (MQ=255)

                                                          tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATTCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGa                                                                            <  1:966382/134‑1 (MQ=255)

                                                          tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGa                                                                            <  1:1766849/134‑1 (MQ=255)

                                                              gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                        <  2:2305019/134‑1 (MQ=255)

                                                               cAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAATCCAGAGTTGCCGAGTTATTCATCTTAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAc                                                                       <  1:405075/134‑1 (MQ=255)

                                                                   tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAGGCAAAAGATATGATATACcgac                                                                   >  2:1895689/1‑134 (MQ=11)

                                                                    ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTACAACAATTACGCAAAAGATATGATATAccgacg                                                                  <  2:493594/134‑5 (MQ=11)

                                                                     tacaGATTTAATGAATTATAAAGATGGAGATATGGCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                 <  2:1467471/131‑1 (MQ=255)

                                                                     tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCTGTGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                 <  2:328388/134‑1 (MQ=255)

                                                                      aaaGATTTAATGAATTATAAAGATGGAGAAATACGCTAAAAACCAGAGGTGCCGAGTTAATCAGCTCAATATCAATTACCTAATGATGATTATAATGTAAAACAATTAAGAAAAAGATATGACATACCACCaaa                                                                >  2:1594749/1‑134 (MQ=17)

                                                                          aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                            <  1:1849754/134‑1 (MQ=255)

                                                                           tttAATGAATTATAAAGATGGAGATATATCATATAATCCATAAATTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaaa                                                           >  2:624353/1‑133 (MQ=255)

                                                                           tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAg                                                           >  2:1078575/1‑134 (MQ=255)

                                                                               aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                       >  1:2025602/1‑134 (MQ=255)

                                                                               aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAAAATTACGTAAAAGATATGACATACAAACAAATAAAGCGcc                                                       >  2:2562845/1‑134 (MQ=255)

                                                                                   aTTATAAATATTGATTTATATTATATAATTTAGAGGTGCTTATTTATTTAGCTTAATATCAATTAATTAATGATGATTATAATTTAAAACAATTACGTAAAAGATATTACATACCAACAAATAAAGCGCCGAAg                                                   <  2:2270875/134‑1 (MQ=14)

                                                                                        aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                              >  1:2340801/1‑134 (MQ=255)

                                                                                        aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                              >  1:186418/1‑134 (MQ=255)

                                                                                              ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAATCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                        <  1:1264058/134‑1 (MQ=255)

                                                                                                       tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACCAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAgg                               >  1:1897510/1‑134 (MQ=255)

                                                                                                              aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAGTAAACAATAAAGCGCCGAAGCTGTTGTTAAAAGGAAAAGGGAAtata                        >  2:2161146/1‑134 (MQ=17)

                                                                                                              aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                        >  1:1291648/1‑134 (MQ=255)

                                                                                                              aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAAAGACATACAAACAAATAAAGCGCCGAAGCAGTTGTTGAAAGGTACAGGGAATTTa                        >  1:909338/1‑134 (MQ=255)

                                                                                                              aTCCAGAGGTGCCGAGTTATTCAGCTCAATACCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                        >  2:562118/1‑134 (MQ=255)

                                                                                                              aTCCAGAGGTGCCGAGATATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAAAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTCGTTGAAAGATACAGGGAATTTa                        >  2:727858/1‑134 (MQ=17)

                                                                                                               tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTATTATTGAAAGGTACAGGGAAATTaa                       >  1:1990668/1‑134 (MQ=255)

                                                                                                                ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTCTAATGTAAAACAATTACGTAAAAGATAAAACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                      >  1:1654387/1‑134 (MQ=255)

                                                                                                                  agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAg                    >  2:641057/1‑134 (MQ=255)

                                                                                                                   gagGTTCAGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATTACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGTTACAGGGAATTTAAAAgg                   <  2:2815967/134‑1 (MQ=255)

                                                                                                                      gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAATTTAAAAGGTtc                >  2:2421258/1‑134 (MQ=255)

                                                                                                                      gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGACGAAGCTGTTGTAGAAAGGTACAGGGAATTTAAAAGGTtc                >  2:2338814/1‑134 (MQ=255)

                                                                                                                       tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca               <  1:1028161/134‑1 (MQ=255)

                                                                                                                        gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              <  1:832149/134‑1 (MQ=255)

                                                                                                                        gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATGTAAAAGGTtcat              >  1:647748/1‑134 (MQ=255)

                                                                                                                        gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTAGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              >  1:427640/1‑134 (MQ=255)

                                                                                                                        gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTGTTGTTGAAAGGTACAGGGCACTAACAAGGTtcat              >  2:58448/1‑134 (MQ=17)

                                                                                                                        gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATAGGACATACTCACCAACAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              >  2:1301461/1‑134 (MQ=17)

                                                                                                                        gCCGAGTTATTCAGCTCAATATCAATTAACTAAGGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCAAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              >  1:2057345/1‑134 (MQ=255)

                                                                                                                          ttaGTTATTCAGCTTAATATCTATTAACTAATGATTATTATAATGTAAAACAATTACGTAAAAGATATGATATACCAACAAATAAAGGGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatca            <  1:709232/132‑1 (MQ=14)

                                                                                                                           gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg           >  2:1801614/1‑134 (MQ=255)

                                                                                                                                aatCAGATCAATATGAATTAACCAATGATGATTATAATATAAAACAATTACGTAAAAGATATGACATACGAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGAGAATTTAAAAGGTTCATCAGTTGGa      <  2:1965385/132‑1 (MQ=14)

                                                                                                                                aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa      <  1:1138462/134‑1 (MQ=255)

                                                                                                                                aTTCAGCTAAATATCAATTAACTAATTATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa      <  1:2366920/134‑1 (MQ=255)

                                                                                                                                 ttCATCTCAATATTAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAATGATATGTCATACCAACAAATAAAGCGCCGAAGGTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat     <  1:1173959/134‑1 (MQ=14)

                                                                                                                                 ttCAGCTCAATATCAATACATTAATGTTGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat     >  2:2512844/1‑134 (MQ=255)

GGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATA  >  USA300TCH1516_ALE/111380‑111645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`