BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,516	C→A	34.5%	Q196K (CAA→AAA)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,516	0	C	A	34.5%	233.2 / 182.1	221	Q196K (CAA→AAA)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (75/66); new base A (29/46); total (106/115)
Fisher's exact test for biased strand distribution p-value = 4.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-02

Only 100 of 223 total aligned reads displayed.

AGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATA  >  USA300TCH1516_ALE/111383‑111645

  ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCaa                                                                                                                                 >  2:1857819/1‑134 (MQ=255)

  ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGATCaa                                                                                                                                 >  1:172794/1‑134 (MQ=255)

   tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTATCAATATATCAATTCTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAAAGTTGCCGACTTATTCAGCTGAat                                                                                                                                >  2:299132/1‑134 (MQ=18)

   tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                <  2:686793/134‑1 (MQ=255)

   tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                <  2:1100040/134‑1 (MQ=255)

    aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                               <  1:2355291/134‑1 (MQ=255)

    aaaGAAGAAAACAGAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                               <  2:642836/134‑1 (MQ=255)

    aaaCAAGAAAACATAAAAAAATAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                                               <  1:362023/134‑1 (MQ=18)

      agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGGAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATc                                                                                                                             <  2:1736583/134‑1 (MQ=255)

       gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                                            >  2:250825/1‑134 (MQ=255)

        aagaaAATAATAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCaa                                                                                                                           <  2:2456609/134‑1 (MQ=255)

          gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTAGAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                                         <  1:22093/134‑1 (MQ=255)

             aaCAGAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATACGGATTGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                      <  2:1306939/134‑1 (MQ=18)

              aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGTAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACt                                                                                                                     <  2:1684189/134‑1 (MQ=255)

              aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                                                     >  2:2418202/1‑134 (MQ=255)

              aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCAACTCAATATCAATTAACt                                                                                                                     >  1:524502/1‑134 (MQ=255)

               cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                                                    <  1:701631/134‑1 (MQ=255)

               cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGATATTAGGATAAATATAAATTAACTa                                                                                                                    >  1:2429680/1‑134 (MQ=18)

                aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaa                                                                                                                   <  2:1420425/134‑1 (MQ=255)

                    aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATGGTCCAGAGGGGCCAAGTTATTCAGATAAATATCAATTAACTAatgat                                                                                                               <  1:420142/134‑1 (MQ=18)

                    aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGGTTTATTCAACGAAATATCAATTAACTAatgat                                                                                                               <  2:347182/134‑1 (MQ=18)

                    aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                               <  1:971162/134‑1 (MQ=255)

                     aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                              <  2:2150809/134‑1 (MQ=255)

                      aaaGAAATTGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAAtgatga                                                                                                             <  2:1916825/134‑1 (MQ=255)

                      aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAAtgatga                                                                                                             >  2:46317/1‑134 (MQ=255)

                       aaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAAACCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                            <  1:2663601/134‑1 (MQ=255)

                         gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                          <  1:2810615/134‑1 (MQ=255)

                         gAAATAGAAAACTTTTAGTTTTTTGCTAAATATATCAATTTTAAAGATTTAATGAATTAAAAAAAAGGAGATATATCATATAATACAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                          <  1:188311/134‑1 (MQ=17)

                          aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAATCCAGAGGTGCCGAGTGATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                         <  1:2295818/134‑1 (MQ=255)

                             tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                      >  1:404597/1‑134 (MQ=255)

                              caaaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATAAATTATAAAGATGGAGATATATCAGATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATg                                                                                                     <  1:1170539/132‑1 (MQ=255)

                                aaaaCTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTGATGAAATAAAAACATGGAGACATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                                                   <  1:1968280/134‑1 (MQ=17)

                                 aaccTTTAAGTTTTTTGCGCAATATATCAATTTTAAAGATTTAATTATTTATAATTATGGTTATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  1:115295/131‑1 (MQ=17)

                                 aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAATGACTTTGTTGATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  2:1723967/134‑1 (MQ=18)

                                 aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  2:764870/134‑1 (MQ=255)

                                 aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  >  1:2273127/1‑134 (MQ=255)

                                 aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  1:949584/134‑1 (MQ=255)

                                   aCTTTAAGTTTTTTGCGTAATATATCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                <  2:454412/134‑1 (MQ=255)

                                     tttAAGTTGTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                              <  2:1989077/134‑1 (MQ=255)

                                      ttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                                                             >  1:1589604/1‑134 (MQ=255)

                                         aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGCTGCCGAATAAACAAACTGAGTATGACTTCACTAATTATGATTATAATTTAAAACAATTa                                                                                          >  2:606022/1‑134 (MQ=25)

                                          gTTTTTTGCGTAATATAGCAATTGTAAAGATTTAATTAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGTCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                         <  2:2211642/134‑1 (MQ=17)

                                          gTTTTTTGCCCCATATCGCAATTTTAAGAATGTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                         <  2:2769080/134‑1 (MQ=14)

                                          gTTTTTTGCACAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                                         <  1:2638143/134‑1 (MQ=255)

                                            tttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgc                                                                                       <  1:325473/134‑2 (MQ=255)

                                             ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                      <  1:1647427/134‑1 (MQ=255)

                                                  cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGTAGATATATCATATAATCCAGAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                 <  1:786746/134‑1 (MQ=255)

                                                  agcAATATAGCAATTTTAAAGATTTAATGAAGTATAAAGATTGAGATATATCATATAATTCAGAGGTGCCGAGTTATTCAGCTCAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                 <  1:758671/133‑1 (MQ=255)

                                                      atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATg                                                                             <  2:1900526/134‑1 (MQ=255)

                                                       tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATATTTCAGAGGTGCCGAGTTATTCGGATAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                            <  1:250825/134‑1 (MQ=14)

                                                       tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                            <  1:1000925/134‑1 (MQ=255)

                                                       tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGa                                                                            <  1:1766849/134‑1 (MQ=255)

                                                           gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                        <  2:2305019/134‑1 (MQ=255)

                                                                tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAGGCAAAAGATATGATATACcgac                                                                   >  2:1895689/1‑134 (MQ=11)

                                                                 ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTACAACAATTACGCAAAAGATATGATATAccgacg                                                                  <  2:493594/134‑5 (MQ=11)

                                                                  tacaGATTTAATGAATTATAAAGATGGAGATATGGCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                 <  2:1467471/131‑1 (MQ=255)

                                                                    aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAt                                                               >  1:806165/1‑134 (MQ=255)

                                                                        tttAATGAATTATAAAGATGGAGATATATCATATAATCCATAAATTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaaa                                                           >  2:624353/1‑133 (MQ=255)

                                                                              gAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                     <  1:1801429/134‑1 (MQ=255)

                                                                              gAAGTATATAGAGGTGGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAATAGATATGACATACCAACAAATAAAGCGCCGa                                                     <  2:586221/134‑1 (MQ=14)

                                                                                aTTATAAATATTGATTTATATTATATAATTTAGAGGTGCTTATTTATTTAGCTTAATATCAATTAATTAATGATGATTATAATTTAAAACAATTACGTAAAAGATATTACATACCAACAAATAAAGCGCCGAAg                                                   <  2:2270875/134‑1 (MQ=14)

                                                                                       aGATGGAGATATATCATATTATCCAGAGGTTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAGATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                            <  1:83068/134‑1 (MQ=255)

                                                                                        gATGGCGGTAGATCAGATAATTCAGAGGGGACCAGTGATTCAGCGCAATAGCAATTAAGTAATGATTATTATAATGTAAAGCAACTACGTAAAAGATATTACATACCAACAAATAAAGCGCCGAAGCTgttgtt                                           <  2:1174723/134‑1 (MQ=14)

                                                                                          tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                         >  1:480260/1‑134 (MQ=255)

                                                                                           ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAATCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                        <  1:1264058/134‑1 (MQ=255)

                                                                                           ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAGCAATTACGAAAAAGAAATGACATACCAACAAATAAAGCGCAGAAGATGTTGTTGaa                                        >  1:398207/1‑134 (MQ=14)

                                                                                              gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAATAATGATGATTCTAATGTAAAACAATTACGTAAAAAATAAGACATACTAACCAATCAAGCGCCGAAACTGTTGTTGCAAgt                                     >  2:1217724/1‑133 (MQ=17)

                                                                                               atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTACCTAATGATGATTACAATGTAAAACAATTACGTAAAAGATATTACATCCCAACAAATACAGCGCCGAAGCAGTTGTTCAAAgct                                    >  2:2807684/1‑132 (MQ=14)

                                                                                               atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                               >  2:1569395/1‑123 (MQ=255)

                                                                                                tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTa                                   >  2:1081164/1‑134 (MQ=255)

                                                                                                 atatCATATAATCCAGCGGTGCTGAGGTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                  >  2:1045489/1‑134 (MQ=255)

                                                                                                    tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATCCCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAga                               >  2:2377460/1‑133 (MQ=255)

                                                                                                    tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACCAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAgg                               >  1:1897510/1‑134 (MQ=255)

                                                                                                      atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGa                             <  1:1857819/134‑1 (MQ=255)

                                                                                                           aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAGTAAACAATAAAGCGCCGAAGCTGTTGTTAAAAGGAAAAGGGAAtata                        >  2:2161146/1‑134 (MQ=17)

                                                                                                           aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                        >  1:1291648/1‑134 (MQ=255)

                                                                                                           aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAAAGACATACAAACAAATAAAGCGCCGAAGCAGTTGTTGAAAGGTACAGGGAATTTa                        >  1:909338/1‑134 (MQ=255)

                                                                                                           aTCCAGAGGTGCCGAGATATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAAAAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTCGTTGAAAGATACAGGGAATTTa                        >  2:727858/1‑134 (MQ=17)

                                                                                                            tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTATTATTGAAAGGTACAGGGAAATTaa                       >  1:1990668/1‑134 (MQ=255)

                                                                                                             ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTCTAATGTAAAACAATTACGTAAAAGATAAAACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                      >  1:1654387/1‑134 (MQ=255)

                                                                                                             ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAGCGCCGAAGCTGTTGTTAAAAGGTACAGGGAATTTaaa                      >  2:2337979/1‑134 (MQ=255)

                                                                                                               agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAg                    >  2:2526673/1‑134 (MQ=255)

                                                                                                                gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCCGTTGTTCAAAGGTACAGGGACTTTAAAAgg                   >  2:1260065/1‑134 (MQ=255)

                                                                                                                  ggTGCCGAGTTATTCAGCTCAATATCCATTAACTAATGATGATTATAATGTCAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCAGAAGCAGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                 >  1:1405331/1‑134 (MQ=255)

                                                                                                                  ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAGGGAATTTAAAAGGtt                 >  1:2391947/1‑134 (MQ=255)

                                                                                                                   gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                >  2:2692712/1‑134 (MQ=255)

                                                                                                                   gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                >  1:2738230/1‑134 (MQ=255)

                                                                                                                   gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCAAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                >  2:1377998/1‑134 (MQ=255)

                                                                                                                    tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca               <  1:1951818/134‑1 (MQ=255)

                                                                                                                    tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca               <  1:1028161/134‑1 (MQ=255)

                                                                                                                     gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              <  2:2435819/134‑1 (MQ=255)

                                                                                                                     gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              <  1:2041097/134‑1 (MQ=255)

                                                                                                                     gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATGTAAAAGGTtcat              >  1:647748/1‑134 (MQ=255)

                                                                                                                     gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAAAAAGAGACGAAAATATTAATAAAAGATACAGGGAATGTAAAAGGTtgat              >  1:2304902/1‑134 (MQ=14)

                                                                                                                     gCCGAGTTATTCAGCTCAATATCAATTAACTAAGGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCAAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              >  1:2057345/1‑134 (MQ=255)

                                                                                                                        gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg           >  2:1801614/1‑134 (MQ=255)

                                                                                                                         aGTTATTCAGCTCCATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAGCAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATGTAAAAGGATCATCAGt          >  2:340784/1‑134 (MQ=255)

                                                                                                                             aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGa      <  1:1138462/134‑1 (MQ=255)

                                                                                                                               tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACCAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata    >  1:1529318/1‑134 (MQ=255)

                                                                                                                                 aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata  >  2:57010/1‑134 (MQ=255)

AGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATA  >  USA300TCH1516_ALE/111383‑111645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`