| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0% | 31.2 / NA | 14 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (6/8); total (6/8) | |||||||||||
TCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTA > USA300TCH1516_ALE/2532174‑2532421 | tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATAAGATAAATGCGCTTActacaa > 2:137823/1‑129 (MQ=255)tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTAAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTAcaccaa > 2:231877/1‑129 (MQ=255) gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc > 1:131939/1‑129 (MQ=255) aaataaaaAAATAGCATTATTAATAAATTAAAAAATTATAGTTTTATTCAGAGAGGGATGGAATAAAATGAACTTTGTAAAGTTGACTTCTGATGATTTGAATGTGTTTACTATATAGCATTTTTctca > 2:118001/1‑129 (MQ=255) tAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAAAGTTAACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt > 1:95410/1‑129 (MQ=255) ataaATGCAAACATTAAAGTTGCAGTGAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAt < 2:152974/129‑1 (MQ=255) atacAAAAATTATAGTTAAATTCAGAGATGGATGGGATAAAATTAACTTTGTAACGTTGACTTCGGATGAGTTTAATGCTTTTACTATAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACa < 1:11389/126‑1 (MQ=255) ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAtt > 2:209352/1‑129 (MQ=255) cAGAGAGGCATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAgg < 2:212699/129‑1 (MQ=255) ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTCATTTAAAAGGCGAtgtg < 2:179493/129‑1 (MQ=255) gatgggatAAAAAGAACTTTGTAACGTCGACTTCAGATGAGTTCAATGAGTTTACTACAAAGCATTTTTCACATTACACACAAACAGCTAGTAATTAGAATGATAGAGTTGATTTAAAAGACGATgcgc > 2:69131/1‑129 (MQ=255) gatAAAAGGAAGTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGTATTTTTCATATTACACATAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt < 1:86309/129‑1 (MQ=255) atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg < 2:19690/129‑1 (MQ=255) gAGTTCAATGAGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt < 1:171382/129‑1 (MQ=255) aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt < 2:131939/129‑1 (MQ=255) tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGTGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACACTGGTCAAGTGATTGTAGGATGCTTa > 2:121036/1‑129 (MQ=255) | TCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTA > USA300TCH1516_ALE/2532174‑2532421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |