Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,607	T→C	D22G (GAT→GGT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,607	0	T	C	100.0%	37.4 / NA	15	D22G (GAT→GGT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/6);  total (9/6)

AATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTT  >  USA300TCH1516_ALE/1744486‑1744723                                                                                                                          |                                                                                                                     aaTGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGGCTTTTGCGTATTCTACAATTTTAACTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTaaa                                                                                                               >  2:178453/1‑129 (MQ=255)                         tCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATc                                                                                       <  1:5393/129‑1 (MQ=255)                                aCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTTTTGCATAGCCATATGCTTCTCTATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACt                                                                                <  1:93899/129‑1 (MQ=255)                                            tctcTGTCTTTTGCGTATTCTACAATTGTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTAt                                                                    <  2:61013/129‑1 (MQ=255)                                                      ttGCGTATTCTACAATTTTATCTGTTGCATAGCCATAAACTTCACCATTAACCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAAt                                                          >  1:10960/1‑129 (MQ=255)                                                                       ttATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTtcctcc                                         >  2:104991/1‑129 (MQ=255)                                                                                gCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTTATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCt                                <  1:129366/129‑1 (MQ=255)                                                                                gCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCt                                >  1:190000/1‑129 (MQ=255)                                                                                   tAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCACCTCAATTGCTCAc                             >  2:152587/1‑129 (MQ=255)                                                                                                    aTTATCCATAATTGTAGTACTACCCTTGAAACTAACAACAGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAAACCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTaa            >  1:112458/1‑128 (MQ=255)                                                                                                    aTTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCAGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTAt            >  1:76432/1‑129 (MQ=255)                                                                                                     ttATCCATAATTGTAGTATTGCTCTTGAAAGTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATc           <  2:93004/129‑1 (MQ=255)                                                                                                         ccATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGACAAAACTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAt       >  1:142768/1‑129 (MQ=255)                                                                                                            tAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGACCAATCTTGAACTTCTAATACGAATTACTTTAAATCCATTAATATTTCCTCCTAAATTACTCACGACAATTGTGACTTTATCCAAtttt    >  1:190038/1‑129 (MQ=255)                                                                                                              aTTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAtttttt  <  1:153077/129‑1 (MQ=255)                                                                                                                          |                                                                                                                     AATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTT  >  USA300TCH1516_ALE/1744486‑1744723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑