Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000731	81	N→G	intergenic (–/‑151)	– / → repA	–/replication protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	81	0	N	G	98.4%	209.4 / NA	64	intergenic (–/‑151)	–/repA	–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (30/32);  minor base T (0/1);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220                                                                                                                                         |                                                                                                                                            atttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAAc                                                                                                                                         <  1:120826‑M1/85‑1 (MQ=255)     tgtaagggtttataaacctttgataaatctatattttctcttttataagtgtATGTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtt                                                                                                                                     >  2:101214‑M1/53‑141 (MQ=255)           ggtttataaccccttgataaagctatattggccgtttgataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttattta                                                                                                                               >  1:120682‑M1/47‑141 (MQ=255)            gtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTttatttat                                                                                                                              >  1:96238‑M1/46‑141 (MQ=255)               tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGATAATAGTAAGAATTTGGTAACAAAACATCTACACACagtttatttatttt                                                                                                                           <  2:59953‑M1/99‑4 (MQ=255)                ataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTAtttatatca                                                                                                                          <  1:1513‑M1/100‑3 (MQ=255)                ataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATaa                                                                                                                          <  1:177220‑M1/100‑1 (MQ=255)                 taaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAt                                                                                                                         >  1:216168‑M1/41‑141 (MQ=255)                  aaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATataaat                                                                                                                        <  2:45161‑M1/102‑3 (MQ=255)                   aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                       >  1:77581‑M1/39‑141 (MQ=255)                           ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAATACCCCTACGACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                               >  2:33328‑M1/31‑141 (MQ=255)                           ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                               >  2:248940‑M1/31‑141 (MQ=255)                           ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                               >  1:51806‑M1/31‑141 (MQ=255)                           ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACt                                                                                                               >  1:252905‑M1/31‑141 (MQ=255)                              aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAATCTGTTTATTTATATAATTTTCGATACTTGt                                                                                                            <  1:135471‑M1/114‑1 (MQ=255)                               agctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGta                                                                                                           >  1:88713‑M1/27‑141 (MQ=255)                               agctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGta                                                                                                           <  1:50931‑M1/115‑1 (MQ=255)                                gctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                          <  1:204518‑M1/116‑1 (MQ=255)                                gctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                          <  1:236949‑M1/116‑1 (MQ=255)                                gctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTAGCAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                          <  1:54684‑M1/116‑1 (MQ=255)                                    tattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtata                                                                                                      <  2:211106‑M1/120‑1 (MQ=255)                                    tattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTAGATACttatattata                                                                                                      <  2:57192‑M1/120‑1 (MQ=255)                                        ttccctattataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGTGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAAt                                                                                                  >  2:16146‑M1/18‑141 (MQ=255)                                          cccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGa                                                                                                <  1:196996‑M1/126‑1 (MQ=255)                                            cttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCTGGGTTTGGTAATAATACTGTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaat                                                                                              >  2:264442‑M1/14‑141 (MQ=255)                                              ttgctaggtttAGTTAAAGTGTTATAAGAAACTTGATATAAGAAAAACGGCAACTACTGTGAATAGTCGGTGTTTGGTAATAAAACGTTTAGTAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                            >  2:177743‑M1/12‑141 (MQ=255)                                               ttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACTCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTATAGAAACTGTTTATTTATATAATTTTCTATACTTGTATTATATAATGaataat                                                                                           <  2:246927‑M1/131‑1 (MQ=255)                                                  taagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATc                                                                                        >  1:127207‑M1/8‑141 (MQ=255)                                                   gggtttAATTAAAGGTCAATCATAAATTTAATATAACAAACATCCCAACTGCTGGTAATAGTCTGGGTTTAGTAAAAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATGATATAATGAATAATATCt                                                                                       >  2:278153‑M1/7‑141 (MQ=255)                                                    agtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCtt                                                                                      <  2:70833‑M1/136‑1 (MQ=255)                                                     gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTa                                                                                     <  2:222980‑M1/137‑1 (MQ=255)                                                      tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCACAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAg                                                                                    >  1:204466‑M1/4‑141 (MQ=255)                                                       tgaTTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGt                                                                                   <  2:273381/139‑1 (MQ=255)                                                           ttAAAGTGCTATAATAAACTTAATATAACAACAACCCCAACCACGGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCaa                                                                               <  1:121508/141‑1 (MQ=255)                                                            tAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAg                                                                              <  1:229385/141‑1 (MQ=255)                                                             aaaGTGCTATAATAACCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGc                                                                             <  1:94120/141‑1 (MQ=255)                                                             aaaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGc                                                                             >  1:18648/1‑141 (MQ=255)                                                                gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                          <  1:50096/141‑1 (MQ=255)                                                                  gCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTa                                                                        <  2:135471/141‑1 (MQ=255)                                                                      taataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATc                                                                    >  2:111102/1‑141 (MQ=255)                                                                             cTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaat                                                             >  1:251443/1‑141 (MQ=255)                                                                                  tataAGAAAAACCCCAATTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATg                                                        <  2:96291/141‑1 (MQ=255)                                                                                      aCAAAAACCCCAAGTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaaa                                                    >  2:176679/1‑141 (MQ=255)                                                                                          aaaCTCCAAATACTGGGAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCta                                                <  1:77245/141‑1 (MQ=255)                                                                                            aCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaa                                              >  1:63609/1‑141 (MQ=255)                                                                                             ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtacat                                             >  2:221209/1‑141 (MQ=255)                                                                                              cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATCTCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                            >  2:86216/1‑141 (MQ=255)                                                                                               ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                           <  1:70833/141‑1 (MQ=255)                                                                                                 aaCTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATaaaa                                         >  1:203805/1‑141 (MQ=255)                                                                                                   ctactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATATTAGTGCAAGCACCTAAATCATTAAATAAATGTAACGCTAAATAAAAAc                                       >  2:233459/1‑141 (MQ=255)                                                                                                    tactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                      <  2:196996/141‑1 (MQ=255)                                                                                                    tactGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                      <  1:14371/141‑1 (MQ=255)                                                                                                     actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                     >  1:93866/1‑141 (MQ=255)                                                                                                              aGTCGGGGTTTGGTAATAAAACGTTTAGCTAAAGATAAGTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAACAACGTTTTATTAcc                            >  2:62705/1‑141 (MQ=255)                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACct                           <  1:147893/141‑1 (MQ=255)                                                                                                                  ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCtt                        >  2:207821/1‑141 (MQ=255)                                                                                                                    ggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATTTAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAc                      <  2:179184/141‑1 (MQ=255)                                                                                                                          gTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACt                <  2:120826/141‑1 (MQ=255)                                                                                                                          gTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACt                <  2:177220/141‑1 (MQ=255)                                                                                                                             ataaAACGTTTATAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaaa             <  1:270588/141‑1 (MQ=255)                                                                                                                             ataaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaaa             <  2:165483/141‑1 (MQ=255)                                                                                                                                aaaGGTTTAGAAACTGTTTATATATATAATTTTCCATACTTTAATTATATACTTAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAata          <  1:268555/141‑1 (MQ=255)                                                                                                                                      ttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGaaa    >  1:129429/1‑141 (MQ=255)                                                                                                                                        aGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAaga  >  2:72580/1‑141 (MQ=255)                                                                                                                                         |                                                                                                                                            ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑