| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,462,758 | G→A | A70T (GCA→ACA) | USA300HOU_RS12680 → | Putative 3‑methyladenine DNA glycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,462,758 | 0 | G | A | 94.7% | 43.6 / NA | 19 | A70T (GCA→ACA) | USA300HOU_RS12680 | Putative 3‑methyladenine DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/7); minor base C (1/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATACCACTCAAACGTATACAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATGCACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTGAACCAGAAGAAGGTTTATCCGCTATGTTCCGTAACAGAGG > USA300TCH1516_ALE/2462620‑2462882 | gATACCACTCAAACGTATACAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATAca > 1:202041/1‑141 (MQ=255) cTCAAACGTATACAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCa > 1:15491/1‑141 (MQ=255) tCAAACGTATACAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCAt > 1:193433/1‑141 (MQ=255) aCGTATACAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATCCAt > 2:218510/1‑141 (MQ=255) tataCAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATTGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACg < 2:91863/141‑1 (MQ=255) taCAGGCTACATCGTGGAAACGGAAGCTTACCTAGGTTTGACTGATCGGGCGGCTCATGGCTATGGCAGTCAAATAACAACTAACGTCACGTCATTATATAAACGTGGTGGTACAATTTCTCCACATGTCATGTATACGCa > 2:47262/1‑141 (MQ=255) cTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACt > 1:118637/1‑141 (MQ=255) tCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGAGATAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTGCTCATTa > 1:256152/1‑141 (MQ=255) ttacttaGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATc < 2:180365/141‑1 (MQ=255) tacttaGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATGAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTAATCATTAATTTTGTAAGAAGATCt > 1:92622/1‑141 (MQ=255) gCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACtt > 2:234783/1‑141 (MQ=255) ggCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGATACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAAtt < 1:101506/141‑1 (MQ=255) gCGGTAAGATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACAGGTCATGCATACGCAGTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTg < 2:4599/141‑1 (MQ=255) ggTAAAATAACAGCTAGAGTCACGTCAGAATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGACGTACTTATCCGCACAATTGaa > 2:160555/1‑141 (MQ=255) acatataGTCAATTCATGATATAAACGTGGTGGTACAATTTATACACATGTGATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTGAACCAGAAGAAgg < 2:99684/135‑1 (MQ=255) tAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTGAACCAGAAGAAGGttt < 2:193433/141‑1 (MQ=255) tatataAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTGAACCAGAAGAAGGTTTATCCGCTATGTTcc < 2:202041/141‑1 (MQ=255) taAACGTGGTGGTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCCCAATTGAACCAGAACAAGGTTTCCCCGCTATGTTCCGTaa > 2:244003/1‑141 (MQ=255) tggtggTACAATTTATACACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTGAACCAGGAGAAGGTTTATCCGCTATGTTCCGTGACAGAgg > 1:70038/1‑141 (MQ=255) | GATACCACTCAAACGTATACAGGCTACATCGTGGAAACGGAAGCTTACTTAGGTTTGAATGATCGTGCGGCTCATGGCTATGGCGGTAAAATAACACCTAAAGTCACGTCATTATATAAACGTGGTGGTACAATTTATGCACATGTCATGCATACGCATTTACTCATTAATTTTGTAACAAAATCTGAAGGTATACCTGAAGGCGTACTTATCCGCGCAATTGAACCAGAAGAAGGTTTATCCGCTATGTTCCGTAACAGAGG > USA300TCH1516_ALE/2462620‑2462882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |