Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 486,180 G→T 5.3% intergenic (+56/‑161) USA300TCH1516_00437 → / → mccA hypothetical protein/O‑acetylserine dependent cystathionine beta‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE486,1800GT 5.3% 954.9 / 8.4 329intergenic (+56/‑161)USA300TCH1516_00437/mccAhypothetical protein/O‑acetylserine dependent cystathionine beta‑synthase
Reads supporting (aligned to +/- strand):  ref base G (189/113);  new base T (23/2);  total (214/115)
Fisher's exact test for biased strand distribution p-value = 2.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 352 total aligned reads displayed.

ATTATTCAGTGGTTGGTATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATCGGCA  >  USA300TCH1516_ALE/486040‑486320
                                                                                                                                            |                                                                                                                                            
attattCAGTGGTTGGTATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTTCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTGTTTgg                                                                                                                                             <  2:3830902/141‑1 (MQ=255)
attattCAGTGGTTGGTATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTtttgtttg                                                                                                                                              >  2:4197196/1‑141 (MQ=255)
attattCAGTGGTTGGTATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTtttgtttg                                                                                                                                              >  2:266386/1‑141 (MQ=255)
    ttCAGTGGTTGGTATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATGTGTGTTGCTCAGGTGTTTTTTCTTTTGGAAt                                                                                                                                          >  2:4413630/1‑141 (MQ=255)
        gTGGTTGGTATTACTTAATTAAGTATGCGATGTCTGTCGTTATTATTTTAGTCTTTATCGTGGAATTATTTAGTTAATATGTAGGAGCATCTGACTCAGAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGaa                                                                                                                                      <  2:1365973/141‑1 (MQ=255)
            ttggtATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAAtt                                                                                                                                  <  2:3976615/141‑1 (MQ=255)
                ttttACTGAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTTCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGt                                                                                                                              <  2:3307665/139‑1 (MQ=255)
                   tACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATAGTGTTTTTTTTTGATGAACATATCGGCTCATCGTACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACt                                                                                                                           <  2:2004917/141‑1 (MQ=255)
                    aCTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTATTCCTg                                                                                                                          >  2:1231683/1‑141 (MQ=255)
                    aCTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTg                                                                                                                          <  1:582990/141‑1 (MQ=255)
                    aCTGAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTg                                                                                                                          <  1:423234/141‑1 (MQ=255)
                       taattaaGTATGCGATACATGTCGGTATTATTGTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATTTGACGCACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGtt                                                                                                                       >  1:2117774/1‑141 (MQ=255)
                        aattaaGTATGCGATGCCTGTCGTTATTATTTTATTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTTTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTc                                                                                                                      <  2:1864799/141‑1 (MQ=255)
                             aGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACACGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAACTTTTGTTCTTGTTCTGCAt                                                                                                                 >  2:424810/1‑141 (MQ=255)
                              gTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTACTATATAAAAGCGGCGTACACCAACATCTTCGAATTCCTCAGGTGTTTTTTTGTTTCGAATTTAAAAATTAGTCCTGGTTTTGACtt                                                                                                                >  2:4291470/1‑141 (MQ=255)
                               tATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTa                                                                                                               >  1:100954/1‑141 (MQ=255)
                               tATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTa                                                                                                               <  1:1980338/141‑1 (MQ=255)
                                aTGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTaa                                                                                                              <  1:1429798/141‑1 (MQ=255)
                                   cGATTCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCg                                                                                                           <  1:1700955/141‑1 (MQ=255)
                                     aTGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAACATTTAGTACTGGTTCTGAATTAATCGaa                                                                                                         >  1:2762906/1‑141 (MQ=255)
                                       gCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTCTCTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAAc                                                                                                       >  1:828092/1‑141 (MQ=255)
                                        ccTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACt                                                                                                      <  1:921667/141‑1 (MQ=255)
                                          tGTCGTTATTATTTTAGTTTTTATAGTGCAATTATTTAGTTAATATATAGAAGTATTTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTa                                                                                                    <  2:1628515/141‑1 (MQ=255)
                                                   tattTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGTAATTGTAAATTTAGTACTGGTTCTTAATTAATCGAACATTAGactcttgc                                                                                           >  2:684617/1‑134 (MQ=255)
                                                       ttAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCaaaa                                                                                       >  2:1095414/1‑141 (MQ=255)
                                                       ttAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCaaaa                                                                                       >  2:528684/1‑141 (MQ=255)
                                                       ttAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCaaaa                                                                                       >  2:4396120/1‑141 (MQ=255)
                                                         aGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATa                                                                                     >  2:2562738/1‑141 (MQ=255)
                                                           tCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTATTCGAAACTTGGTTATTTACACAATAAc                                                                                   >  2:2064344/1‑141 (MQ=255)
                                                             tttATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCACAATAACaa                                                                                 >  2:1818105/1‑141 (MQ=255)
                                                              ttATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACaaa                                                                                >  1:500751/1‑141 (MQ=255)
                                                                aTCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGTAATTGAAAATTTATTACTGTTTCTGAATTAATCTAAACTTAGATAATTGCAAAATAACAAAAt                                                                              >  2:1562598/1‑141 (MQ=255)
                                                                    tGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCg                                                                          >  1:2819990/1‑141 (MQ=255)
                                                                    tGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCg                                                                          >  2:3366779/1‑141 (MQ=255)
                                                                      cAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTTAATTAATCTAAACTTAGATAATTGCAAAATAACAAAATgttgtt                                                                        >  2:3643380/1‑141 (MQ=255)
                                                                       aaTTATTTAGTTAATATATAGAAGTATTTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGttt                                                                       <  2:285657/141‑1 (MQ=255)
                                                                         ttattTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTtat                                                                     >  1:3595529/1‑141 (MQ=255)
                                                                         ttattTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTtat                                                                     >  1:2852265/1‑141 (MQ=255)
                                                                         ttattTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTtat                                                                     <  2:2524008/141‑1 (MQ=255)
                                                                           attTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATaa                                                                   <  2:2818442/141‑1 (MQ=255)
                                                                            tttAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTtatact                                                                  >  1:3004209/1‑139 (MQ=255)
                                                                             ttAGTTAATATATAGAAGCATCTGACACACAAATATTTGTGTTGCTCAGGTGTTTTTTTTTTTTTTATTGAAACTTTAGTATTGGTTTTGAATTAATCGAAAGTTAGATAATTGAAAAATAACAAAATGTAGTTTATAAtt                                                                 >  2:3892849/1‑141 (MQ=255)
                                                                              tAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTc                                                                <  1:1052930/141‑1 (MQ=255)
                                                                              tAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGGTCTGGAGTGATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTc                                                                >  1:1230902/1‑141 (MQ=255)
                                                                               aGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCt                                                               <  2:1340891/141‑1 (MQ=255)
                                                                               aGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTAAATTGTGTGATTAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAGTTGCAAAATAACAAAATGTCGTTTATAATTCt                                                               >  2:3583332/1‑141 (MQ=255)
                                                                                gTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCtt                                                              >  1:847998/1‑141 (MQ=255)
                                                                                gTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCtt                                                              >  1:1795751/1‑141 (MQ=255)
                                                                                     tatataGAAGCATCTTACATACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTg                                                         <  2:653337/141‑1 (MQ=255)
                                                                                      atataGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGa                                                        >  2:2718645/1‑141 (MQ=255)
                                                                                        ataGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAGTTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGaaa                                                      >  2:4072635/1‑141 (MQ=255)
                                                                                         taGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGaaaa                                                     <  1:266386/141‑1 (MQ=255)
                                                                                         taGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGaaaa                                                     >  2:2236430/1‑141 (MQ=255)
                                                                                           ggagtaTCTGACACAGAAGTATTGGTGTTGATCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATTGAAAATTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGt                                                   <  2:2985401/136‑1 (MQ=255)
                                                                                            aaGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTa                                                  >  1:316561/1‑141 (MQ=255)
                                                                                              gCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATATTTCTTATCTGAAAAGTAAg                                                >  1:2472682/1‑141 (MQ=255)
                                                                                              gCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAg                                                >  1:240112/1‑141 (MQ=255)
                                                                                               cATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGa                                               >  1:1273266/1‑141 (MQ=255)
                                                                                               cATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGa                                               <  1:4082052/141‑1 (MQ=255)
                                                                                                aTCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTATTATCTGAAAAGTAAGaa                                              >  1:332815/1‑141 (MQ=255)
                                                                                                 tgtGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAAt                                             <  2:2472564/139‑1 (MQ=255)
                                                                                                 tCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAAt                                             <  1:2562738/141‑1 (MQ=255)
                                                                                                 tCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAAATTAGTTACCACAAAAATAACAAAATGACATTTATAATTCTTATCTGAAAAGTAAGAAt                                             >  1:2063519/1‑141 (MQ=255)
                                                                                                 tCTGACACAAAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAAt                                             <  1:3889284/141‑1 (MQ=255)
                                                                                                  cTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGTTTTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAACTAACAAAATGTCGTTTATAATTCTTTTCTCTAAAGTAAGAAtt                                            >  2:2135424/1‑141 (MQ=255)
                                                                                                   tGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTa                                           >  1:1587572/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAATATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAATTTGTCGTTTATAATTTTTATCTTTAACGTAAGCATTaa                                          >  2:1298287/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTGTTATCTGAAAAGTAAGAATTaa                                          >  1:1109902/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTaa                                          >  1:678819/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTaa                                          >  2:2599898/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTaa                                          >  1:2490370/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTGAGATAATGGCTAAATAACAAAATGTAGCTTATGACACGGATCTGAAAAGTAAGAATTaa                                          >  1:4134234/1‑141 (MQ=255)
                                                                                                    gACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGGACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTaa                                          >  1:132990/1‑141 (MQ=255)
                                                                                                    aacacacAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTTTAATTTTATATTTAGTACTGGTTCTTAATTCATCGTTCCTTCTTTAATTGCCAATTAACCACATGTCTGTTTTAATTCTTTTCTGCATATTAATAATTaa                                          >  2:1587051/2‑141 (MQ=255)
                                                                                                     acacacAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGTTTCTGAATTAATCGAAATTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTATTATCTGAATATTAACAATTaaa                                         >  2:3110809/1‑141 (MQ=255)
                                                                                                     acacacAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTaaa                                         <  1:1942603/141‑1 (MQ=255)
                                                                                                      aacataAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTATGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTaaaa                                        <  2:2287054/140‑1 (MQ=255)
                                                                                                       acacaAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATCACAAAATGTCGTTTATAATTCTTATCTGCAAAGTAATAATTAACAt                                       >  2:769783/1‑141 (MQ=255)
                                                                                                       acacaAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAAt                                       <  1:403710/141‑1 (MQ=255)
                                                                                                            aGTAATTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTgaga                                  >  1:1873512/1‑141 (MQ=255)
                                                                                                              tATTTGTGTTGGTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACa                                <  2:1693619/141‑1 (MQ=255)
                                                                                                               aTTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAACTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACAt                               >  1:3431348/1‑141 (MQ=255)
                                                                                                                tttGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTTAATTAATCGAAACTTAGATAATTGCAAACTAACAAAATGTCGTTTATAATTCCTATCTGAAAAGTATGAATAAAAATTGAGACAtt                              >  1:3958043/1‑140 (MQ=255)
                                                                                                                tttGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATg                              <  1:3479816/141‑1 (MQ=255)
                                                                                                                tttGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATg                              >  1:1465310/1‑141 (MQ=255)
                                                                                                                  tgtgTTGCTCAGGTTTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATgaa                            <  1:4387550/141‑1 (MQ=255)
                                                                                                                  tgtgTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTTAGACATgaa                            >  2:1176420/1‑141 (MQ=255)
                                                                                                                  tgtgTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAACTAAGAATTAAAATTGAGACATgaa                            >  1:2308271/1‑141 (MQ=255)
                                                                                                                   gtgtTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATgaag                           <  2:943255/141‑1 (MQ=255)
                                                                                                                   gtgtTGCTAAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATgaag                           <  2:2966997/141‑1 (MQ=255)
                                                                                                                        gCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAga                      >  1:3799020/1‑140 (MQ=255)
                                                                                                                          tCAAGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGTTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGtt                    <  1:3766635/141‑1 (MQ=255)
                                                                                                                              gtgtTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTttcat                <  2:864284/141‑1 (MQ=255)
                                                                                                                                       tgtttgGAATTGAAAATTTAGTACTGGGTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGAt       >  1:2022749/1‑141 (MQ=255)
                                                                                                                                       tgtttaGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGAt       <  2:3760939/141‑1 (MQ=255)
                                                                                                                                       ggtttgGATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATc      <  1:746616/140‑1 (MQ=255)
                                                                                                                                        gtttgGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATc      <  1:2418856/141‑1 (MQ=255)
                                                                                                                                          ttgGAGTTGAAAATTTAGTACTGTGTCTGAATTAATAGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATCgg    <  2:485614/141‑1 (MQ=255)
                                                                                                                                            ggAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATCGGCa  >  2:3218365/1‑141 (MQ=255)
                                                                                                                                            ggAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATCGGCa  >  1:4060611/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATTATTCAGTGGTTGGTATTACTTAATTAAGTATGCGATGCCTGTCGTTATTATTTTAGTCTTTATCGTGCAATTATTTAGTTAATATATAGAAGCATCTGACACACAAGTATTTGTGTTGCTCAGGTGTTTTTTTGTTTGGAATTGAAAATTTAGTACTGGTTCTGAATTAATCGAAACTTAGATAATTGCAAAATAACAAAATGTCGTTTATAATTCTTATCTGAAAAGTAAGAATTAAAATTGAGACATGAAGAAAGGTTTCATTCATTTGATCGGCA  >  USA300TCH1516_ALE/486040‑486320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: