Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,513 T→C 5.4% D1205D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5130TC 5.4% 947.7 / 7.1 396D1205D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (152/202);  new base C (25/3);  total (185/211)
Fisher's exact test for biased strand distribution p-value = 1.44e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 405 total aligned reads displayed.

GATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTC  >  USA300TCH1516_ALE/618373‑618653
                                                                                                                                                 |                                                                                                                                            
gattcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGAt                                                                                                                                              <  2:2965111/141‑1 (MQ=255)
       atAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagaca                                                                                                                                       >  1:2420655/1‑141 (MQ=255)
        tAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATCGCGACTCAAACTCAAAAAAGGCCTCCGA‑‑CTCAGATAGCGACTCAGACtcagccac                                                                                                                                      >  2:2659086/1‑140 (MQ=17)
        gagagatgcaggttcaGATAGAGATTCAGACAGAGATGAAGATTCAGATAGAGACTGAGATTCAGACAGCGACTGAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacag                                                                                                                                      <  2:2519262/137‑1 (MQ=14)
         aGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagc                                                                                                                                     >  1:321450/1‑141 (MQ=255)
           cGATTCAGATTCAGACAGAGACTCAGATAGTGATTGCGAAATAATGGTGTACACAGATCACACGTCTGACCTAAAGTCAGACCGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcga                                                                                                                                   >  1:1046210/1‑141 (MQ=9)
            gattcagatcaaGATAGAGATTCAGATAGTGATTCAGATTCAGATAGAGACTCAGATTCAGATAGTGACTCAGATTCAGACTGTGACTCAGACTCAGATAGTGATTCAGA‑‑CTCATATAGCGACTCAGATtcagacagcgac                                                                                                                                  <  2:2736588/141‑1 (MQ=17)
             attcagattcagACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTGAGCTTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATTGTGGTTCAGA‑‑GTCGTATAGCGACTAAGAAacagacagcgact                                                                                                                                 >  2:3911908/1‑141 (MQ=17)
              ttcagattcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactg                                                                                                                                >  1:727628/1‑140 (MQ=255)
              cacagatacagaCAGAGATACACAGAGAGATCCAGACTCAACTCGGGACTCAGATTCAGATAGCGACACGGAGTCAGACAGAGACACAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactc                                                                                                                                <  2:1511664/139‑1 (MQ=14)
               tcagattcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGAATCAGATtcagacagcgaatca                                                                                                                               >  1:1095173/1‑141 (MQ=255)
                  gactcagaGAGAGACTGAGAGACTGATACAGACTCAGATAGCGACACAGAGTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGTGACTCAGATtcagacagcgactcagac                                                                                                                            <  2:185851/138‑1 (MQ=17)
                   attcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagact                                                                                                                           >  1:1868352/1‑141 (MQ=255)
                     tcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGAGACGCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGCCTCAGATtcacacagcgaatcagactca                                                                                                                         >  2:963763/1‑141 (MQ=14)
                      cagacagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgaatcagacccag                                                                                                                        >  2:4001808/1‑141 (MQ=255)
                          cagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagactcagacag                                                                                                                    >  1:2154160/1‑141 (MQ=255)
                          cagaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagactcagacag                                                                                                                    >  1:4319607/1‑141 (MQ=255)
                            gagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagactcagacagcg                                                                                                                  >  1:865869/1‑141 (MQ=255)
                            gagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgacccatcctcagccacag                                                                                                                  >  2:904848/1‑138 (MQ=14)
                                cTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACACCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagactcagacagcgactc                                                                                                              <  1:4251412/141‑1 (MQ=255)
                                  cAGATAGTGATTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACGCAGACTCAGATAGTGACTCAGA‑‑CTCAGATAGCGACTCAGATtccgacagggactcagacacagaaagcgaccgag                                                                                                            >  2:2213880/1‑137 (MQ=11)
                                    aaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTAAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagactcagacagcgactcagac                                                                                                          <  1:2523921/140‑1 (MQ=255)
                                     aTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTcagacagcgactcagactcagacagcgactcagact                                                                                                         >  1:4013184/1‑141 (MQ=255)
                                     aTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTcagacagcgactcagactcagacagcgactcagact                                                                                                         <  1:3337126/141‑1 (MQ=255)
                                      tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCagacagcgactcagactcagacagcgactcagactc                                                                                                        <  1:1291161/141‑1 (MQ=255)
                                      tAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGACTCATCCTCACAGAGAGCTATCA‑‑CTAAATTCATCGACTCAGACtcagacagcgactcacactcagacagcgactcagagac                                                                                                        >  2:4289019/1‑138 (MQ=2)
                                         tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGAcagcgactcagactcagacagcgactcagactcaga                                                                                                     <  1:3747802/141‑1 (MQ=255)
                                         ggagacAGAATCAGATAGCGACTCAGAGTCAGATAGCGATTAAGATTCAGACAGAGACACAGACTCAGATAGTGATTCAGC‑‑CTCAGATAGCGACTCAGATTCAGAcagcgactcagactcagacagcgactcagactcaga                                                                                                     <  2:2030523/136‑1 (MQ=14)
                                          gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACCCAGAGAGCGGCTCAGACTCAGAt                                                                                                    >  1:2760348/1‑141 (MQ=255)
                                               aGACTCAGATAGCGACTCACATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGa                                                                                               <  1:2473545/141‑1 (MQ=255)
                                                gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGAc                                                                                              <  1:2666003/141‑1 (MQ=255)
                                                 aCTCAGATGGCGACACAGATGCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACt                                                                                             <  2:1679308/141‑1 (MQ=255)
                                                 aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACACAGACAGCGACTCAGACTCAGATAGCGAca                                                                                             >  2:3246918/1‑140 (MQ=14)
                                                  cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGAGAGCGACTCAGACTCAGCCAGCGACTCAGACGCAGATAGTGACtc                                                                                            >  1:1952782/1‑141 (MQ=255)
                                                   tCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCACTGGAGTTCAGAGGCGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtca                                                                                           <  1:1025644/141‑1 (MQ=17)
                                                    cAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcag                                                                                          >  1:921003/1‑141 (MQ=255)
                                                         agcgactcagataccgacagtgacgcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattca                                                                                     <  2:2924484/141‑1 (MQ=255)
                                                                  agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCCGACTCAGATAGCGa                                                                             >  1:35974/1‑141 (MQ=255)
                                                                      tcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCa                                                                         >  1:2826081/1‑141 (MQ=255)
                                                                       cagacagcgactcagattcagacagcgactcagaCTCAGATAGTGACTCAGA‑‑CTCAGATAGCGACTCAGACTCAGACAGCGATAGAGACTCAGACACCGACGCAGACTAAGAGGGCGAGGCAGACACAGATAGCGAcagag                                                                        >  2:2839328/1‑137 (MQ=11)
                                                                         gacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGac                                                                      >  1:4109385/1‑140 (MQ=255)
                                                                           cagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTc                                                                    <  1:3639871/141‑1 (MQ=255)
                                                                            agcgactcggattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCg                                                                   >  1:3769664/1‑141 (MQ=255)
                                                                            agcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACCGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACCCAGATTCAGATAGCGCCTCAGATTCg                                                                   >  1:3436959/1‑141 (MQ=255)
                                                                             gcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGGGACGCAGACGCAGAGGGCGACGCAGGCTCAGATAGTGACTCAGAGTGAGATAGCGACTCAGATTggg                                                                  >  1:3065423/1‑141 (MQ=17)
                                                                              cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                 >  1:143645/1‑141 (MQ=255)
                                                                              cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGAATCAGAATCAATTAGTGAGTCAGATTCAGATAGCGACTCAGATTCGGa                                                                 >  1:1206984/1‑141 (MQ=18)
                                                                               tgactcagattcagattgagattcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                 <  2:10635/140‑1 (MQ=14)
                                                                                aaatcagattaagaaagccaatcagaCTCAGACAGCGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGAc                                                                <  2:307803/138‑1 (MQ=14)
                                                                                 actcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACa                                                               >  1:1612328/1‑141 (MQ=255)
                                                                                  ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                              <  1:3347792/141‑1 (MQ=255)
                                                                                  ctcagattcagacagcgactcagaCTCAGATAGCGACTCAGA‑‑CTCAGATAGCGACTCAGACTCAGACAGCGACGCAGACTCAGAGAGCGACTCAGACTCAGCTAGTGACTCAGATGCAGATAGCGACTCAGAATCAGACAg                                                              >  2:1206228/1‑141 (MQ=11)
                                                                                    cagattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCg                                                            <  1:562954/141‑1 (MQ=255)
                                                                                      gatttagactgcgattcagaATCAGATGGTGATGCAGC‑‑ATACGGTCACCACTCAGTGTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                          <  2:3126267/141‑1 (MQ=14)
                                                                                      gattcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACGCAGATTCGGACAGCGAt                                                          >  1:452620/1‑141 (MQ=255)
                                                                                      gattcagacagcgactcagaCTCAGATAGCGATTCAGA‑‑CTCAGATAGCGACTCAGCTTCAGATCGCGACTCAGACTCAGATAGCCAATCGGACTCGGATGGCGACTCAGAGTCAGACGGACAGTCAGAATCAGACAGCGac                                                          >  2:3415727/1‑140 (MQ=2)
                                                                                       attcagacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACACAGACTCAGATAGCGACACAGACTCAGATAGCGACTCAGACTCAGACAGcagaa                                                         >  2:277485/1‑137 (MQ=11)
                                                                                       attcagacagagactcagaCTCAGATAGTGAGTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAtt                                                         <  2:2298340/141‑1 (MQ=255)
                                                                                           agacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                     <  1:2600024/141‑1 (MQ=255)
                                                                                            gacagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGACTCAGAGTCACATAGCGAGGCAGAGTCACATAGCCACTCAGaa                                                    >  2:1265169/1‑140 (MQ=11)
                                                                                              cagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                  >  1:753756/1‑141 (MQ=255)
                                                                                              cagcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                  <  1:352198/141‑1 (MQ=255)
                                                                                               agcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGCTAGCGACTCAGATTCGGACAGCGATTCAGACTcc                                                 >  1:1525637/1‑140 (MQ=255)
                                                                                               agcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                 >  1:393029/1‑141 (MQ=255)
                                                                                               agcgactcagaCTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                 >  1:318507/1‑141 (MQ=255)
                                                                                                    ctcagactcagaGAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                            <  1:2110519/141‑1 (MQ=255)
                                                                                                      cagactcagaTAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                          >  1:4163210/1‑141 (MQ=255)
                                                                                                       agactcagcTAGTTATCCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGAGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                         <  1:3899631/141‑1 (MQ=255)
                                                                                                         actcagaTAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTAAGAATCGGACAGCGATTCAGACTCAGATAGCGACt                                       >  2:142292/1‑141 (MQ=255)
                                                                                                         actcagaTAGTGATGCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACt                                       <  2:659321/141‑1 (MQ=255)
                                                                                                          ctcagaTAGTGACTCAGA‑‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACGCAGAGTCATATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                      <  1:2213004/141‑1 (MQ=14)
                                                                                                          ctcagaAGGGGATGCAGA‑‑CTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                      <  2:4209709/141‑1 (MQ=14)
                                                                                                               acaGTGACTCAGT‑‑CTCATATAGCGACCCAGCTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagatt                                 <  2:3765086/139‑1 (MQ=14)
                                                                                                                 aGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGGCAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATGGTGACTCAGATTCAGATAGCGACTCAGGTTCGGACAGCGATTCAGACTCAGCCAGCGACgcagaatca                               >  1:809572/1‑141 (MQ=14)
                                                                                                                      ttCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGTGACTAAGACTGAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                          <  1:3008293/141‑1 (MQ=255)
                                                                                                                         aGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                       <  1:4235779/141‑1 (MQ=255)
                                                                                                                         aGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                       <  1:963763/141‑1 (MQ=255)
                                                                                                                         aGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                       <  1:1988228/141‑1 (MQ=255)
                                                                                                                          gA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCCGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                      >  1:383717/1‑141 (MQ=255)
                                                                                                                          gA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                      >  1:229234/1‑141 (MQ=255)
                                                                                                                           a‑‑CTCAGATAGCGACTCAGATtcagacagcgactcagactcagacagcgactcagac                                                                                                          >  1:674408/1‑56 (MQ=35)
                                                                                                                           a‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGACTCAGATAGCGACTCAGACTCGGAAGGAGAGGAGGGCTCAGGTAGCGACTCAGATTCAGATGGAGAtt                     >  1:116908/1‑141 (MQ=14)
                                                                                                                               tCAGATAGCGACTCAGATtcagacagcgactcagactcagacag                                                                                                                    >  1:3259365/1‑44 (MQ=40)
                                                                                                                               tCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACt                                                                           >  2:776956/1‑85 (MQ=255)
                                                                                                                                 aGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGAGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                 <  1:2351953/141‑1 (MQ=255)
                                                                                                                                  ggtgaGCGACGCGGATTCGGACAGCGCCTCAGACTCAGACAGCGACTCAGACTCAGACAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                 <  2:35974/137‑1 (MQ=14)
                                                                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc              <  1:2902376/141‑1 (MQ=255)
                                                                                                                                    tAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc              <  1:3458435/141‑1 (MQ=255)
                                                                                                                                     gggCGATTTAGATTCAGAAGGAGACTAAGGTTCAGAAAGGGACTCAGAATCAGATAGAGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGCATCAGATTCAGATAGCGATTCGGACTc              <  2:1609749/139‑1 (MQ=14)
                                                                                                                                     aggCGACTCAGATTGAGACAGCGACGCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTc              <  2:849523/139‑1 (MQ=255)
                                                                                                                                       gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAg            <  1:1358273/141‑1 (MQ=255)
                                                                                                                                          aCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATa         <  1:1486222/141‑1 (MQ=255)
                                                                                                                                           ctaaGATTGAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg        <  2:1022017/138‑1 (MQ=255)
                                                                                                                                           cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg        >  1:3968393/1‑141 (MQ=255)
                                                                                                                                           cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAATCAGACCAGGACAGCGATTGAGACTCGGAAAGCGACTCAGATACAAATAGCGATACGGGCTAAGAtta        >  2:3335864/1‑139 (MQ=14)
                                                                                                                                             cAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg      >  1:2621575/1‑141 (MQ=255)
                                                                                                                                              aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAATCAGACTTCGAGAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGa     >  2:525232/1‑141 (MQ=14)
                                                                                                                                               gATTCAGCGCGCGAAGCAGACACAGACAGCGACACAGAATCAGATAGAGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc    <  2:2401498/141‑1 (MQ=14)
                                                                                                                                                aTTCAGAcagcgactcagactcagacagcgactcagactcaga                                                                                                     >  1:1738187/1‑43 (MQ=35)
                                                                                                                                                aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt   >  1:602051/1‑141 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
GATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGA‑‑CTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTC  >  USA300TCH1516_ALE/618373‑618653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: