Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,693 T→C 6.8% D1265D (GAT→GAC)  sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6930TC 6.8% 1021.1 / 9.6 450D1265D (GAT→GAC) sdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (203/194);  new base C (30/8);  total (240/210)
Fisher's exact test for biased strand distribution p-value = 1.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 456 total aligned reads displayed.

GATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTC  >  USA300TCH1516_ALE/618553‑618833
                                                                                                                                            |                                                                                                                                            
   aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                                                           >  1:1170493/1‑141 (MQ=255)
   aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                                                           >  1:3300813/1‑141 (MQ=255)
   aGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATCCACACACTGATAGCAAAACAGACTCa                                                                                                                                           >  2:1081338/1‑141 (MQ=11)
     tGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                                                                                         >  2:278750/1‑141 (MQ=255)
      gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                                                                                        >  1:1607468/1‑141 (MQ=255)
           agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                                                                   <  2:320847/141‑1 (MQ=255)
           agattcagatAGCCATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                                                                   <  2:1227644/141‑1 (MQ=255)
             attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAcc                                                                                                                                 >  1:4089328/1‑140 (MQ=255)
             attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATATCGACTAGGAGTCAGATAGCGACTCAGGTTCAGATCGCGAGTGAGAATCAGATCTCGACTCAAGTCCAGAcctcgaag                                                                                                                                 >  2:2119843/1‑134 (MQ=2)
                  gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAt                                                                                                                            >  1:3729527/1‑141 (MQ=255)
                     aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                                                                         >  1:1202846/1‑141 (MQ=255)
                     aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                                                                         <  1:524733/141‑1 (MQ=255)
                     aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTTCGATTCAGACAACGAAACAGATTCg                                                                                                                         >  2:3734169/1‑141 (MQ=14)
                         aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCggctc                                                                                                                     >  1:2042919/1‑138 (MQ=255)
                         aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATa                                                                                                                     >  1:339208/1‑141 (MQ=255)
                          ctgaGAATCGGACAGCGATTCATGCTCAGATTTCTATTTATTTTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACGCAGATAGAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                                                                                                    <  1:68323/138‑1 (MQ=17)
                          cTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACCGCGACTCAGATGCCGATAg                                                                                                                    >  2:76693/1‑141 (MQ=255)
                             aGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCgc                                                                                                                 >  1:1386331/1‑140 (MQ=255)
                              gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                                                                                                                <  1:4412919/141‑1 (MQ=255)
                                ttCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTc                                                                                                              <  1:419375/141‑1 (MQ=255)
                                ctcgtagaGCGATTGAGATTCTGATAGCGTGTGAGATTCGGATAGCGACTAGGACTCAGCTAGCGACTCAGATTCAGATAGTGATTCAGAGTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTc                                                                                                              <  2:1518147/134‑1 (MQ=17)
                                         cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGa                                                                                                     >  1:2536382/1‑141 (MQ=255)
                                         cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAATCAGACTCAGATATTGATTAAGCCTCTGGGGACAACGCAGATTCAGACAGCGCATCAGATTCGGATAGCGACTCAGATCCAGa                                                                                                     >  1:842379/1‑141 (MQ=17)
                                             tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc                                                                                                 <  1:3643292/141‑1 (MQ=255)
                                             tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATCCAGACAGCGACTCAGATTCGAATAGCCACTCAGATAcagccagc                                                                                                 >  2:4015358/1‑141 (MQ=17)
                                              cAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCg                                                                                                >  1:2769190/1‑141 (MQ=255)
                                              cAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACGCAGATTCGGCTAGCGACGCAGATTCAGACAGCg                                                                                                >  2:4242965/1‑141 (MQ=255)
                                                gACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCCGATAGCAACTCAGACACAAACAGCCAc                                                                                              >  2:3259055/1‑141 (MQ=14)
                                                 aCTCAGATGGAGACGCAGATTCAGATAGAGATTCCGACTCAGATAGAGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACt                                                                                             <  2:3047775/141‑1 (MQ=17)
                                                 aCTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACt                                                                                             <  1:1571969/141‑1 (MQ=255)
                                                     aGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                                                         >  1:3080738/1‑141 (MQ=255)
                                                       aTAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATACACCTAGCGACCCAGATTCAGATAGCGACACACACt                                                                                       >  2:3556035/1‑141 (MQ=11)
                                                         aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCg                                                                                     >  1:4288904/1‑141 (MQ=255)
                                                         aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCg                                                                                     >  1:3243476/1‑141 (MQ=255)
                                                           cGACTCAGATTCAGATTGCGGTTCTTACACAGCTTGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                                                                                   <  2:489906/141‑1 (MQ=18)
                                                           cGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                                                                                   <  1:2745169/141‑1 (MQ=255)
                                                            gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAt                                                                                  >  1:2839210/1‑141 (MQ=255)
                                                              cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAg                                                                                <  1:3791477/141‑1 (MQ=255)
                                                               tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                                                                               >  1:1039245/1‑141 (MQ=255)
                                                               tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGagcga                                                                               >  2:1411657/1‑137 (MQ=255)
                                                               tcagattcagatAGCGATTCGGACTCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                                                                               <  1:2687674/141‑1 (MQ=255)
                                                               acagtttcggatAGTGAGACTGATTCAGATAGAGACACAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                                                                               <  2:2853190/140‑1 (MQ=17)
                                                                  gactcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                                                                            <  1:1423271/138‑1 (MQ=255)
                                                                  gaatcagagAGCGACTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                                                                            <  1:981043/138‑1 (MQ=255)
                                                                    ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGCTAGCGTCTCAGAGTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                          <  1:856971/141‑1 (MQ=255)
                                                                    cacagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                          <  2:2420655/139‑1 (MQ=255)
                                                                        gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAc                                                                      >  1:2587060/1‑141 (MQ=255)
                                                                        gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGACCCAGACTCGGATAGCGCTCCAGaa                                                                      >  2:868699/1‑140 (MQ=17)
                                                                          tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                    >  1:424705/1‑141 (MQ=255)
                                                                          tAGCGATTCGGACGCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                    <  1:3911908/141‑1 (MQ=255)
                                                                           aGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAAAGTCACGGACACTCAGACAGCGACTCAGACTCGGATAGACATTCAGACTCa                                                                   >  2:1782748/1‑141 (MQ=17)
                                                                             cGAGTCGGACTCAGATAGCGACTCAGATTCAGACAGTGATTCAGACGCAGATAGCGACTCAGATTCAGACCGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGCCTCGGATAGTGATTCAGACTCAGa                                                                 <  1:908238/141‑1 (MQ=18)
                                                                                ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                                                              >  1:2996464/1‑141 (MQ=255)
                                                                                ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAg                                                              >  2:3829888/1‑141 (MQ=255)
                                                                                    gACTCAGATAGCGACTCAGATTCAGAGAGTGATTCAGACTCAGATAGCGACTCAGATCCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgac                                                          <  1:1656732/141‑1 (MQ=255)
                                                                                     aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgact                                                         >  1:2637273/1‑141 (MQ=255)
                                                                                     aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATACAGACACAGATAGCgaca                                                         >  2:1152722/1‑140 (MQ=255)
                                                                                      cTCAGATAGTGCTTCTGATTCAGATAGCGAGTCAGACTCAGATAGCGCCTCAGACTCAGATAGCGACTCAGACTCCGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                                                        <  2:2530490/141‑1 (MQ=14)
                                                                                      cTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                                                        <  1:790929/141‑1 (MQ=255)
                                                                                      cTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                                                        >  1:3140867/1‑141 (MQ=255)
                                                                                        cAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCAACCCAGACTCGGCTAGTGACTCACACCCAGATAGCgactcag                                                      >  2:268557/1‑141 (MQ=17)
                                                                                             aGCGACTCAGATTCAGATAGTGATTGAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGCTAGTGATTCAGACTCAGAGAGCgactcacactca                                                 >  2:1601560/1‑141 (MQ=17)
                                                                                              ccGAGTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGactcagactcag                                                <  1:2213880/140‑1 (MQ=255)
                                                                                               cGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGActcagactcaga                                               >  1:2320289/1‑141 (MQ=255)
                                                                                                  cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                            >  1:4388132/1‑141 (MQ=255)
                                                                                                  cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                            <  1:28629/141‑1 (MQ=255)
                                                                                                  cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTACGACACCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                            >  1:3451746/1‑141 (MQ=255)
                                                                                                  cTCAGATTCACATACTGACTCAGCCTCAGACAGCGACTCTGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                                            <  2:2968886/141‑1 (MQ=18)
                                                                                                   tcagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGACAGCGACGCAGACTCGGATAGCGATTCAGACTCACCTAGCGAagccgactcagacaac                                           >  2:3401935/1‑137 (MQ=17)
                                                                                                       attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATATCGACCCAGATTCAGACAGCCACTCAGACTCGGATAGTGCTTCAGACTCAGATAGCCACTCACACTCCGATAGTgata                                       >  2:2913550/1‑140 (MQ=18)
                                                                                                       attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgatt                                       >  1:4017865/1‑141 (MQ=255)
                                                                                                       attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATACAGACACAGACTCACATAGCgatt                                       >  2:1664668/1‑141 (MQ=17)
                                                                                                        ttcagatAGTGATTCACACTCAGATAGCGACTCATATTCACACAGCGACTCATATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattc                                      <  1:2387630/141‑1 (MQ=255)
                                                                                                           agatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcaga                                   <  1:3540457/141‑1 (MQ=255)
                                                                                                            gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTTAGATAGCGACTCAGATTCAGACAGCGACCCAGACTCGGATAGTGATTCAGACCCAGATAGAGACACAAACTCAGATAGCgaaccacat                                  >  2:1858632/1‑134 (MQ=17)
                                                                                                            gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagat                                  >  1:3973582/1‑141 (MQ=255)
                                                                                                              tactGACTCAGACGCAGAGAGCGACTCAGATTCAGACAGCGTCTCAGATGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattc                                <  2:4132331/138‑1 (MQ=14)
                                                                                                                 ggacgcAGACTCAGAGATAGACGCAGATTCAGAGAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAttcagattcaga                             <  1:2108905/136‑1 (MQ=17)
                                                                                                                  gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATtcagattcagat                            <  1:4137983/141‑1 (MQ=255)
                                                                                                                    ttCAGACTCAGATAGCGACTCAGATTCAGACCGCGTCTGTAATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                          <  1:1878395/141‑1 (MQ=17)
                                                                                                                    ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                          <  1:1326444/141‑1 (MQ=255)
                                                                                                                    tgcAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                          <  1:2894203/139‑1 (MQ=255)
                                                                                                                     tgtgACTCTGATAGTGACTCAGATTCAGACAGCGAGTCAGATTCGGATAGCGACACAGATTCAGAAAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGc                         <  2:3591738/138‑1 (MQ=17)
                                                                                                                       aGACTCAGATAGCGATGTAGAGTCATACAGCGACTCAGAGTCTGATTGCCACCCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCga                       <  2:809572/141‑1 (MQ=11)
                                                                                                                       aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATCGCGACTCAGAGTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGAGACACACACTCAGATAGCGAACCAGCGTCAGATACCga                       >  2:711500/1‑141 (MQ=17)
                                                                                                                       aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCga                       >  1:1090501/1‑141 (MQ=255)
                                                                                                                        gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgac                      <  1:3062775/141‑1 (MQ=255)
                                                                                                                         aaccAGAGAGGGAGTCAGATTCATACAGCGACTCAGAGTCAGATAGCGACTCAGATCCAGACAGCGACTCAGACTCAGACAGTGAGTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgact                     <  2:1957417/138‑1 (MQ=14)
                                                                                                                         aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTGTCAAACAAAAAGCGAATCAGATTCAAATAGCGATTCAGATTCAGATAGCgact                     >  1:4356104/1‑141 (MQ=18)
                                                                                                                            cAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCACACTCAGATAGCgactcaa                  >  2:576956/1‑140 (MQ=255)
                                                                                                                                tAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAAAGTGATTCAGACCCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAAATAGCgacgcagactc              >  2:3323614/1‑141 (MQ=255)
                                                                                                                                   ggcctcagactcagaCAGCGAATCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGActcagactcaga           <  2:2335870/138‑1 (MQ=255)
                                                                                                                                     aCTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACa         <  1:3496560/141‑1 (MQ=255)
                                                                                                                                      ttCAGATACATACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAg        <  2:1386331/140‑1 (MQ=255)
                                                                                                                                      tctaGATTCATTCTGCTACTCATATTCTGTTATCTACTCATATTCATACTTCGACTCAGACTCTGATAGTGATTCAGACTCAGATAGCGACTCAGACTCTTATTTTCATTTTGATTCAGATAGCGACTCAGACTCAGACAg        <  2:310890/138‑1 (MQ=9)
                                                                                                                                      cTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAg        <  1:2452865/141‑1 (MQ=255)
                                                                                                                                          gATTGAGAAAGTGATTCGTATTCGGAGAGCGCCTCAGATTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt    <  2:3968393/141‑1 (MQ=14)
                                                                                                                                          gATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt    <  1:1806361/141‑1 (MQ=255)
                                                                                                                                           aTTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAtt   >  1:1502888/1‑141 (MQ=255)
                                                                                                                                            ttCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtc  >  1:3824880/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTC  >  USA300TCH1516_ALE/618553‑618833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑