Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 622,359 | T→C | 6.8% | D974D (GAT→GAC) | sdrE → | Serine‑aspartate repeat‑containing protein E |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 622,359 | 0 | T | C | 6.8% | 1063.7 / 23.1 | 429 | D974D (GAT→GAC) | sdrE | Serine‑aspartate repeat‑containing protein E |
Reads supporting (aligned to +/- strand): ref base T (201/183); new base C (23/11); total (231/198) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.06e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 475 total aligned reads displayed.
GATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTC > USA300TCH1516_ALE/622219‑622499 | acttCACACTTGACAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACtc‑agatt < 1:719232/139‑1 (MQ=255) tCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACtc‑agattcag > 1:2971708/1‑141 (MQ=255) tCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACtc‑agattcag > 1:3686658/1‑141 (MQ=255) acacTTGATAACGGATACTTCGAATGACATACAACAGACAGCGATTCAGACTCAGATAGTGACTCAGACATCGACTCTGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCCGACAGCGACtc‑agactccgat > 2:1823542/1‑141 (MQ=25) acacTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAGTAAGACACCG‑AT‑ACAGATTCAGACAGCGACta‑cgattcagat > 2:43822/1‑141 (MQ=37) acacTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TAAGATTCAGACAGCGACtc‑agattcagat > 1:127247/1‑141 (MQ=255) cTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGACGCAGACAGc > 2:1705202/1‑141 (MQ=255) ttGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCg > 1:515497/1‑141 (MQ=255) tGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGa > 1:979534/1‑141 (MQ=255) tGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGa > 2:2948882/1‑141 (MQ=255) tAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTAAATAAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTc < 1:3222094/141‑1 (MQ=255) ccGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAg < 1:1941657/140‑1 (MQ=255) aCGGATATCTGGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAg < 2:281682/141‑1 (MQ=255) aCGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAg < 2:2218091/141‑1 (MQ=255) gATACTTCGAAGAAGAGACAGCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCG‑AT‑TCAGACTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGact > 2:2717938/1‑139 (MQ=255) gATACTCCGAAGAAGATACATCAGACGGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGAtt < 1:1135168/141‑1 (MQ=255) aTACTACGAAGAAGATACATCAGACAGCGATGCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTc < 2:3719327/141‑1 (MQ=255) tACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCg > 1:993798/1‑141 (MQ=255) tACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGACTCg > 1:2785651/1‑141 (MQ=255) aCTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCgg < 1:4167280/141‑1 (MQ=255) tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACa < 1:2001317/141‑1 (MQ=255) cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATCGCGACTGCGATTCGGACAg > 2:3211636/1‑141 (MQ=255) cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAg < 1:2644226/141‑1 (MQ=255) gaagaaGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTcagccagc > 2:2317296/1‑141 (MQ=255) gaagaaGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTAAGACAGc > 2:1246462/1‑141 (MQ=255) agaagaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGa < 1:3116354/141‑1 (MQ=255) gaagaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGAt < 1:4220133/141‑1 (MQ=255) agaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTc > 1:3296989/1‑141 (MQ=255) gaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa > 1:327018/1‑141 (MQ=255) aTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg > 1:3740604/1‑141 (MQ=255) aTACATCAGACAGCGATTCAGACTCAGATAGAGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCAGACAGGGATACaa > 2:3833229/1‑140 (MQ=18) tCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa < 1:2426018/141‑1 (MQ=255) tCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa < 1:2948518/141‑1 (MQ=255) acAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa < 2:1627341/140‑1 (MQ=255) cAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg < 1:1608267/141‑1 (MQ=255) gACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGAG‑AT‑TCAGATTCAGACAGCGACTC‑CGACTCAGATAGCGACACAGACTCAGACAGCGATCCAGCCTCACAt > 2:581008/1‑141 (MQ=17) cAGCGATTCAGACTCAGAGAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg < 1:1098601/141‑1 (MQ=14) gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg > 1:2318035/1‑141 (MQ=255) tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca > 1:4413215/1‑141 (MQ=255) tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca < 1:2146700/141‑1 (MQ=255) tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca < 1:3555055/141‑1 (MQ=255) tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca < 1:2174497/141‑1 (MQ=255) tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGAATCAGACTCAGACAGTGCATATAAAATTTA‑A‑AGCGAGTA‑AGATTCAGATAGCGACTAAGAATCAGCCAGAGACGCAGACTCAGATAGCGCCtaa > 2:2170797/1‑139 (MQ=12) aGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga < 1:1402273/141‑1 (MQ=255) gACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat > 1:2869095/1‑141 (MQ=255) cTCAGATAGTGACTCAGACAGCAACTCAGACTCAAACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattc < 2:4178493/141‑1 (MQ=255) cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TTAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagcttccg > 1:3366338/1‑139 (MQ=255) cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCTGATAGCGACtcagattcag > 1:2474768/1‑141 (MQ=255) cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcag > 1:3516899/1‑141 (MQ=255) aGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AC‑TCAGACTCAGACAGCGACTC‑AGACGCAGAAAGCGACACAGACACTGAAACCAATTCACACTCGAACAGCGACtcagacgcgga > 2:2094970/1‑135 (MQ=2) aGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtgagatacagt > 2:2998839/1‑140 (MQ=17) aGATAGTGACTCAGACAGCGACTCAGACACAGACTTCTTCTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga < 1:1876068/141‑1 (MQ=17) gataaggacacagacagcgacgcagactcagaaAGCGACTCAGACTCAGACAGTG‑AG‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat < 2:3137921‑M2/108‑1 (MQ=255) gATAGTGACTCACACAGCGAATCAGACTGAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat < 2:3616590/141‑1 (MQ=255) tAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg < 1:4064668/141‑1 (MQ=255) tAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg > 1:564033/1‑141 (MQ=255) tAGTGACTCAGACAGCGACTCAGACGCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg < 1:1038638/141‑1 (MQ=255) aGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc < 1:2220612/141‑1 (MQ=255) gTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGACTCAGACAGCGACTC‑AGACTCAGATAGCGACTCAGACACGGACAGCGACGCAGACCCAGATAGCGACtccgatacagaaacaa > 2:3366775/1‑136 (MQ=14) ctcagacagcgactcagactcagacagcgactcagaCTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGACAGCgctgc > 2:3417693/1‑137 (MQ=255) cagacagcgactcagactcagacagcgactcagactCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcag < 1:1895788/141‑1 (MQ=255) acagcgactcagacttagataatcacgcacactcagACAGCG‑AT‑GCAGATTCAGACAGCGACTC‑AGACGCAGATAGCGACTCAGATACAGACAGCGATCCAGACTCAGATAGCGACTAAGATGCAGCTAGCGAGccagacg > 2:2033718/1‑140 (MQ=2) acagcgactcagactcagacagcgactcagactcagACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGa > 1:1714071/1‑97 (MQ=255) gactcagacagcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGAc < 1:3860855/141‑1 (MQ=255) cagacagcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcag > 1:1416767/1‑141 (MQ=255) agacagcgactaagacgcagacagGG‑AT‑TCAAAGTCAGAAAGCGACTT‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcaga < 2:788965/141‑1 (MQ=14) gacagcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagat > 1:4007331/1‑141 (MQ=255) agcgactcaggctcagacagTG‑AT‑TCAGATTCAGAAAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca < 2:260016/141‑1 (MQ=255) agcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcggattca > 1:4304159/1‑141 (MQ=255) agcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca > 1:1581432/1‑141 (MQ=255) agcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca < 1:1792255/141‑1 (MQ=255) cgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGCCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattcaga < 1:4366122/141‑1 (MQ=255) cgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGATTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattcaga > 1:635038/1‑141 (MQ=255) ctcagactcagaaagGG‑AT‑TCAGATTCAGACAGCGGCCC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAg < 1:2384891/141‑1 (MQ=14) tcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGc > 1:120970/1‑141 (MQ=255) ggactcatgcagGC‑AT‑TTAGATACAGACACGGAATG‑AGATTGAGTTAGCGATTCAGATTCGTACATCGATTCAGACTCATATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa < 2:3266522/140‑1 (MQ=14) agactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGCATCAGAGAGCGACTCAGATACGGACAGCGACTCAGACTCAGATAGCGACTCAGACACAGCGAGCGAGTCAGACTCAGAGAACGACTCACATTGAGAGAGCGa > 2:4306925/1‑141 (MQ=11) gactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt < 1:1537461/141‑1 (MQ=255) gagacagTG‑AT‑TCAGATTCAGACAGAG‑CTCTATATTCAGATAGCTACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg < 2:3301046/140‑1 (MQ=17) gacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCTGATTTGGGTAGCCACGCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc > 1:2085220/1‑141 (MQ=17) agTG‑AA‑TCAGATTCAGACAGAGACTC‑AGATTCAGACAGCGAAGAAGATTAAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa < 2:1333984/141‑1 (MQ=14) tG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa < 1:2265207/141‑1 (MQ=255) g‑at‑tcagattcaGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGaa > 1:493566/1‑140 (MQ=255) g‑at‑tcagattcaGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAc < 1:3821125/141‑1 (MQ=255) g‑at‑tcagattcaGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTAAGATAGCGATTCGGACGCAgta > 2:145552/1‑139 (MQ=255) t‑tcagattcagaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg < 1:1814119/141‑1 (MQ=255) tcagattcagaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACACCGACAGCGACTCAGATTCAGATAGCCATTCCGACTCTGACacc > 2:1510957/1‑139 (MQ=14) gtatcagaCAGCGACTC‑AGACTCAGATAGCGACTGAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt < 2:4007331/138‑1 (MQ=255) ttcagaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc > 1:2538723/1‑141 (MQ=255) agaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACtcag > 2:1802752/1‑68 (MQ=255) gaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc > 1:3161119/1‑141 (MQ=255) gaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc < 1:76320/141‑1 (MQ=255) cAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc < 1:1496880/141‑1 (MQ=255) ggCGACTC‑AGATTCAGATAGCGAATCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa < 1:3641807/140‑1 (MQ=255) cgcgcCTC‑AGACGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa < 1:4130779/140‑1 (MQ=255) aGCGACTC‑AGATTCAGAGAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa < 1:670974/141‑1 (MQ=255) cGACTC‑CGATCCAGATATCTCCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa < 1:918329/141‑1 (MQ=14) gACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAt < 1:2709427/141‑1 (MQ=255) agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga > 1:3467630/1‑141 (MQ=255) attcagatAGCGACTCAGCTTCGGACAGGGACACAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACAGAGACCGCGACTGAGATAGAGACAGCGAAGCGGACTGAGACAGCGACAGAGACACAGATAGCgact > 2:2474761/1‑141 (MQ=9) | GATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTC > USA300TCH1516_ALE/622219‑622499 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |