Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,521 T→C 5.8% D1028D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5210TC 5.8% 1146.1 / 11.1 468D1028D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (211/213);  new base C (29/4);  total (245/223)
Fisher's exact test for biased strand distribution p-value = 1.49e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 513 total aligned reads displayed.

GACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTC  >  USA300TCH1516_ALE/622381‑622661
                                                                                                                                            |                                                                                                                                            
gatggCGAGTCGGACGGAGATGTCGACTAGGAGTAAGATAGCGATTAAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                                                                              <  2:1364442/137‑1 (MQ=14)
gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                                                                              >  1:3264833/1‑141 (MQ=255)
  cAGCGATTCAGACTCAGATAGCGCCGCAGAGTCAGATAGCGATTCAGACTCAGACAGCCACTGAGAATCAGGTAGCGATCCGGACTCATACAGCGAATAAGAATCAGATAGCGACTCAGACTCAGACAGCGACacggattc                                                                                                                                            >  2:2238818/1‑141 (MQ=14)
    gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATCGCGACTCAGACTCAAACAGCGactcagactcag                                                                                                                                          >  1:3357555/1‑141 (MQ=255)
    gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtccgattcag                                                                                                                                          >  1:719124/1‑141 (MQ=255)
    gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGAGTCGGAGTGAGACAGCGATTCAGACTCAGATAGCGACTCAGCCTTAGACAGAGACtctgagtcag                                                                                                                                          >  2:1792186/1‑141 (MQ=14)
     cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                                                                                                                                         >  1:3042922/1‑141 (MQ=255)
     cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAAACTCAGATAGCGACTCAGACTCAGACAGCGACtcagaaacaga                                                                                                                                         >  2:1420190/1‑141 (MQ=14)
      cacgcAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCCGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                                                                                                                                        <  1:1225989/137‑1 (MQ=255)
        cgcAGACTCAGAAAGCGACGCAGATGCAGAAAGCGATGCAGATGCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAg                                                                                                                                      <  2:781957/139‑1 (MQ=14)
         tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                                                                     >  1:1453189/1‑141 (MQ=255)
         tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                                                                     >  1:3808708/1‑141 (MQ=255)
         tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                                                                     >  1:2998056/1‑141 (MQ=255)
          cAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGACAGCGACTCAGGCTCAGACAGCGACTCAGACTCAGACACCGACACAGATGCAGACAGCg                                                                                                                                    >  1:1294485/1‑141 (MQ=11)
             acgcAGACAGCGACGTAAATACAGAAAGCGAGACAGACTGAGACAGCGAGTCAGAGTCAGATAGCGAGACGGACTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt                                                                                                                                 <  2:3819071/138‑1 (MQ=11)
             aCTCAGAAAGTGACGCAAATGCAGAGAGGGATACAGATAAAGAAAGCGACTCAGAATCAGACAGCGATTCAGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt                                                                                                                                 <  2:3189074/141‑1 (MQ=11)
                 aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATCGCGAATcaca                                                                                                                             >  2:264133/1‑138 (MQ=255)
                  gacaGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                            <  1:1526635/138‑1 (MQ=255)
                     aGCGACTCAGATTCAGATAGCGATTCAGACTTTTTTAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGAGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCGGACGCa                                                                                                                         >  2:2024423/1‑141 (MQ=14)
                     aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGAGATTCAGACTCAGATAGCCACTCCGACTCAGCCAGCGACTCCAACTCAGACAGCGACTCAGAccaa                                                                                                                         >  2:2711045/1‑138 (MQ=11)
                      gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACCGCAACAGCGACCCAAAATCAGATAGCGACACGGACtgca                                                                                                                        >  2:1525178/1‑138 (MQ=14)
                       cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                       <  1:1491161/141‑1 (MQ=255)
                        gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                      >  1:4188065/1‑141 (MQ=255)
                        gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCACATTCAGATAGCGATTCGGAcacgaat                                                                                                                      >  1:404536/1‑135 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    <  2:714142/141‑1 (MQ=255)
                          cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                    >  1:3704948/1‑141 (MQ=255)
                           tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACGCAGACAGCGACTCAGATTCAGCTACCGGCTCGGACGGAGATAga                                                                                                                   >  1:1797771/1‑140 (MQ=14)
                             agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                 <  1:3555028/141‑1 (MQ=255)
                             agattcagatAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACACCGACTCAGATTCAGATAGCGACTCGGCCTCAGACAGCGa                                                                                                                 >  2:3697776/1‑141 (MQ=14)
                              gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                >  1:2027402/1‑141 (MQ=255)
                               attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                                                                               >  1:994159/1‑141 (MQ=255)
                               attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAACAACACAGAATCAGCTAGCGATTCAGACACAGATacccaga                                                                                                               >  2:2397433/1‑135 (MQ=14)
                                  cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                            >  1:1717025/1‑141 (MQ=255)
                                  cagatAGCGATGAAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                            <  2:2868430/141‑1 (MQ=255)
                                   ctatcgCGATTCAGACGCAGACAGCGACTCAGATCCAGAGAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGATTCGGACTCAGATAGCGACTCAGa                                                                                                           <  1:350550/136‑1 (MQ=14)
                                   agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGa                                                                                                           <  1:2674918/141‑1 (MQ=255)
                                   agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTcccc                                                                                                           >  2:1698261/1‑138 (MQ=255)
                                      tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                        >  1:1767367/1‑141 (MQ=255)
                                      tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                        <  1:1601342/141‑1 (MQ=255)
                                       aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGATTCAGATAGCGACTCCGATTCa                                                                                                       >  1:195557/1‑141 (MQ=255)
                                         cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGGTTTAGACGCAAACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                     >  1:1023317/1‑141 (MQ=17)
                                         cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTGAGATAGCGATTCGGAGTCAGATAGCGACTCAGATTCAGa                                                                                                     >  1:2557990/1‑141 (MQ=255)
                                         cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                     >  1:3616083/1‑141 (MQ=255)
                                          gATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGATAGCGACTCAGATTCAGAc                                                                                                    <  1:1636542/141‑1 (MQ=255)
                                          gATTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGAGATTCAGACTCAGATAGCGACTCAGacaccgac                                                                                                    >  2:974818/1‑134 (MQ=14)
                                          gATTAAGACTCAGAGAGCGACTCAGATTCAGATAGCGATTCGGAATCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                                                                                    <  2:4388542/141‑1 (MQ=255)
                                            ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGCCTCAGATTCAgcccg                                                                                                  >  1:884409/1‑137 (MQ=255)
                                            ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                  >  1:53455/1‑141 (MQ=255)
                                              cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCCGACAGCg                                                                                                >  1:2793326/1‑141 (MQ=255)
                                                gactgagacAGTGACTCAGATGCAGACAGCGATTCGGACTCAGACAGCGATTCAGATTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                              <  2:352955/141‑1 (MQ=14)
                                                 acgcagatATCGACTCGGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                             <  2:4239930/138‑1 (MQ=14)
                                                    cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                          >  1:2484525/1‑141 (MQ=255)
                                                     agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCACACAGCGATCCAGa                                                                                         >  1:2738193/1‑141 (MQ=255)
                                                     agacAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                         <  1:3958229/141‑1 (MQ=255)
                                                      gacAGCGACTAAGATTCAGATAGCGACTCTGACTCAGACTGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                        <  2:1595477/141‑1 (MQ=17)
                                                        cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                      >  1:2439886/1‑141 (MQ=255)
                                                          gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAg                                                                                    >  1:4323550/1‑141 (MQ=255)
                                                           cGACTCACATTCATAGCGCGCATAAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                   <  1:3407738/141‑1 (MQ=17)
                                                              ttcagattcagacAGCGATTCGGATGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                <  2:2108851/140‑1 (MQ=14)
                                                                  gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                            <  1:581008/141‑1 (MQ=255)
                                                                  gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAGATTCAGACCGCAACTCACACTCAGAGCGCgac                                                                            >  2:610886/1‑141 (MQ=14)
                                                                        gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGATTCAGATAGCGATACAGACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGAAAACgactccgac                                                                      >  2:1112590/1‑141 (MQ=17)
                                                                          aaGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGCCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                    <  1:3320651/140‑1 (MQ=255)
                                                                           aGCGATGCGGACTCAGACAGCGATTCAGGCTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGAGAGCGCTTCACACTCAGAGAGAGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCgactcagactca                                                                   <  2:4136539/141‑1 (MQ=14)
                                                                            gCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                  >  1:3090446/1‑141 (MQ=255)
                                                                                ttCGGACGCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACCGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                              <  1:168344/141‑1 (MQ=255)
                                                                                ctCGGGCACAGGCAGCGAATCAGACTCAGACAGCAAATCAGACTACGACAGCGACGAAGATTCAGAAAGCGATTCGGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                              <  2:2971708/140‑1 (MQ=14)
                                                                                  cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                            <  1:2955295/141‑1 (MQ=255)
                                                                                          gtcAGCGACTCAGCCTCAGAGAGCGACTCACGCTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATCCAGACATCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat                                                    <  2:2793326/139‑1 (MQ=14)
                                                                                            cAGCGATTCAGACTCAGATTGCTACTCATACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCCATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagaatc                                                  >  2:3861211/1‑141 (MQ=17)
                                                                                            cAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                  >  1:2398786/1‑141 (MQ=255)
                                                                                            cAGCGACGCAGACTCAGATAGCGACTCACACTCAGACAGCGACTCAGATTCAGTTAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                  <  2:2538723/141‑1 (MQ=255)
                                                                                                  ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAg                                            <  1:2591909/141‑1 (MQ=255)
                                                                                                    cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCg                                          >  1:447217/1‑141 (MQ=255)
                                                                                                      gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACCGCgac                                        >  1:1115038/1‑141 (MQ=255)
                                                                                                      gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgac                                        >  1:2286882/1‑141 (MQ=255)
                                                                                                        tcgcgATACCGAATCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                      <  2:4004071/137‑1 (MQ=255)
                                                                                                        cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                      <  1:3118522/141‑1 (MQ=255)
                                                                                                            gATAGCGACTCAGCCTCAGACAGCGACGCAGAATCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagac                                  <  1:1401272/141‑1 (MQ=255)
                                                                                                              tAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactc                                >  1:3853460/1‑141 (MQ=255)
                                                                                                                  gactcagactcaGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGAt                            >  1:2869029/1‑141 (MQ=255)
                                                                                                                    ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAg                          <  1:3766724/141‑1 (MQ=255)
                                                                                                                    ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAg                          >  1:2918093/1‑141 (MQ=255)
                                                                                                                       agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGa                       <  1:687455/141‑1 (MQ=255)
                                                                                                                           tcagacAGCGACTCAGATTCCGATAGCGATTCGGACTCAGATAGCGACTCAGATACAGAAAAAGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCa                   >  1:220711/1‑141 (MQ=17)
                                                                                                                           tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCa                   >  1:271512/1‑141 (MQ=255)
                                                                                                                           tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGAAAGCAACTCAGACTCAGATAGAGACTCa                   >  2:1181336/1‑141 (MQ=255)
                                                                                                                            cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACACAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTAAAATGCAGAAACCGACACAGACTCAGAGAGCGAATCAg                  >  2:3596518/1‑141 (MQ=14)
                                                                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCCACTCAGCTTc              >  2:375357/1‑141 (MQ=255)
                                                                                                                                 aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCg             >  1:1368557/1‑141 (MQ=255)
                                                                                                                                  gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATCGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg            >  1:2364478/1‑141 (MQ=255)
                                                                                                                                  gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCCGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACACAGATTCgg            >  2:1256834/1‑141 (MQ=255)
                                                                                                                                  gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg            >  1:3173245/1‑141 (MQ=255)
                                                                                                                                      cTCATATTCAGATTGCGAATCAGACTCAGACTGCCACTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAg        <  2:3075849/141‑1 (MQ=17)
                                                                                                                                      cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCACATTCGAACAg        >  2:3491873/1‑141 (MQ=255)
                                                                                                                                        cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCg      >  1:1191683/1‑141 (MQ=255)
                                                                                                                                         agagtcagatACCGATTGAGACTGTGATAGCGAATGAGACTCTGACAGCGAATCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCCGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGa     <  2:1855671/141‑1 (MQ=14)
                                                                                                                                           attcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCGGACAGCGACt   >  1:2460709/1‑141 (MQ=255)
                                                                                                                                           attcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAgagaga   >  2:1235140/1‑139 (MQ=255)
                                                                                                                                            ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTc  <  1:2144840/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTC  >  USA300TCH1516_ALE/622381‑622661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑