Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,533 T→C 6.0% D1032D (GAT→GAC)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5330TC 6.0% 1265.8 / 15.8 507D1032D (GAT→GAC) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (223/224);  new base C (34/9);  total (267/240)
Fisher's exact test for biased strand distribution p-value = 2.04e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 518 total aligned reads displayed.

GACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAG  >  USA300TCH1516_ALE/622393‑622673
                                                                                                                                               |                                                                                                                                            
 aCTCAGATAGCGAGTCAGATTCAGAGAGCGATACAGATTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGAtt                                                                                                                                             <  2:1416767/141‑1 (MQ=14)
         aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCa                                                                                                                                     <  1:2317296/141‑1 (MQ=255)
          gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAg                                                                                                                                    >  1:1618450/1‑141 (MQ=255)
          gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACCGCAACAGCGACCCAAAATCAGATAG‑‑‑CGACACGGACtgca                                                                                                                                    >  2:1525178/1‑138 (MQ=14)
           cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGa                                                                                                                                   <  2:2085220/141‑1 (MQ=255)
           cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGa                                                                                                                                   >  1:3792152/1‑141 (MQ=255)
           cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAG‑‑‑CGACTCGGACTCAGa                                                                                                                                   >  2:3180317/1‑141 (MQ=255)
             acccagagtcagatAGCGACTAAGACGAAGAAAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACCGAGACTCAGAGTCAGATAG‑‑‑CGATTCGGACTCAGATa                                                                                                                                 <  2:2457945/138‑1 (MQ=14)
              ctccaattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAg                                                                                                                                <  2:365404/136‑1 (MQ=255)
              cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAg                                                                                                                                >  1:1133370/1‑141 (MQ=255)
                cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGCTTCAGAGAG‑‑‑CGATTCGGACTCAGATAACg                                                                                                                              >  1:2764460/1‑141 (MQ=255)
                 agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAAATTCAGATAG‑‑‑CGACTCGGACTCAGACAGCGa                                                                                                                             >  2:3697858/1‑141 (MQ=11)
                  gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGAc                                                                                                                            <  1:1400815/141‑1 (MQ=255)
                  gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGAc                                                                                                                            >  1:2027402/1‑141 (MQ=255)
                   attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCCTCCGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACt                                                                                                                           <  1:3101108/141‑1 (MQ=255)
                   attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACt                                                                                                                           >  1:4004071/1‑141 (MQ=255)
                    ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTc                                                                                                                          <  1:1114979/141‑1 (MQ=255)
                    ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTc                                                                                                                          <  1:660844/141‑1 (MQ=255)
                      cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAg                                                                                                                        <  1:2668294/141‑1 (MQ=255)
                       agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGa                                                                                                                       <  1:2161721/141‑1 (MQ=255)
                           aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGATTCAGATAGCGACTCCGATTCa                                                                                                                   >  1:195557/1‑141 (MQ=255)
                           aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCa                                                                                                                   >  1:4245777/1‑141 (MQ=255)
                            gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCCGACTCAGAAAGCGACTCAGATTCaa                                                                                                                  >  2:3516787/1‑140 (MQ=255)
                             cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGGTTTAGACGCAAACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                                 >  1:1023317/1‑141 (MQ=17)
                             cGATGCAGACTCAGACAGCGAATCCGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                                 <  2:3571761/141‑1 (MQ=255)
                              gATTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑AGATTCAGACTCAGATAGCGACTCAGacaccgac                                                                                                                >  2:974818/1‑134 (MQ=14)
                                ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                              <  1:937355/141‑1 (MQ=255)
                                ttCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                              <  1:2525148/141‑1 (MQ=255)
                                 tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                                             <  1:4122053/141‑1 (MQ=255)
                                  cagactcagacAGCGACTCCGATTCAGAAAGCGATGCGGACGCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                                            <  1:361004/141‑1 (MQ=255)
                                  cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAG‑‑‑CGATTCAGACTCAGATAGAGACCCAAATTCAGACaaga                                                                                                            >  2:256686/1‑138 (MQ=14)
                                   gcacgcagaaAGCGTCTAAGAGTAAGACAGCGATAGCGACTCAGACAGAGATTCAGACTGAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                                           <  2:743645/136‑1 (MQ=14)
                                   agactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                                                           >  1:2461578/1‑141 (MQ=255)
                                    gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGGTAGCGACTCAGATTCAGACAGCGAt                                                                                                          >  1:273354/1‑141 (MQ=255)
                                     actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                         >  1:2570976/1‑141 (MQ=255)
                                     actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAG‑‑‑CGACTCCGACTCAGACAACGACACAGACACAGACAACGact                                                                                                         >  2:2842765/1‑139 (MQ=14)
                                     acgcagatATCGACTCGGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                         <  2:4239930/138‑1 (MQ=14)
                                      ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                        <  1:2440809/141‑1 (MQ=255)
                                      cgccgacAGCGAAGCAGATTCAGATAGCGATTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                        <  1:143216/137‑1 (MQ=255)
                                       ccagacAGCGTCGCACACTCAGATGGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCTGACACCGTCAGCGACGCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                                                                                       <  1:1475595/140‑1 (MQ=17)
                                         agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                                     >  1:3935691/1‑141 (MQ=255)
                                             aGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAgacagcgactcagactcagacag‑‑‑cgactcagactcagacagcgactcagactcagacagcgactcagactca                                                                                                 >  2:3027485/1‑141 (MQ=11)
                                               cGACTCACATTCATAGCGCGCATAAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                                                                               <  1:3407738/141‑1 (MQ=17)
                                                  ctgagattcagatAGTGATGATGATTAAGACAGGGAGTCTGTGGAAGATAGTGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                            <  2:2785793/138‑1 (MQ=14)
                                                  agcagattcagagAGAGATGCAGACTCAGCAAGCGAGTCAGATTCAGAAAGCGACTGAGACTCAGACGGCGATTCAGATTCAGCTAG‑‑‑CGATTCGGACTCAGATAGCGAGTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                            <  2:1673512/139‑1 (MQ=14)
                                                  aacagattcaggtAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                            <  1:573729/139‑1 (MQ=255)
                                                    cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                                          <  1:516714/141‑1 (MQ=255)
                                                    cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGAATC‑‑ATT‑AGATTGGTTCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                                          >  1:4375733/1‑141 (MQ=17)
                                                      gattcagatAGCGATTCGGACTCATACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCATATAG‑‑‑CGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                        <  1:1789797/141‑1 (MQ=255)
                                                      gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAACgac                                                                                        >  1:3552040/1‑141 (MQ=255)
                                                      gaatcagctAGCGACTCAGAATCCGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGATTCAGACAG‑‑‑CGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                        <  2:1581432/138‑1 (MQ=11)
                                                          cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                    >  1:1223369/1‑141 (MQ=255)
                                                            gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                  <  1:2418474/141‑1 (MQ=255)
                                                            gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                  <  1:3703062/141‑1 (MQ=255)
                                                                gCGATTCGGACTCAGACAGCGATGCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCAACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                              >  1:3053429/1‑141 (MQ=255)
                                                                 cgagttggACTCAGACAGCGAGGCGGACTCAGAAAGCGACTGAGACTCAGAGAGCGACTAAGATTCCGAAAG‑‑‑CGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                             <  2:671070/135‑1 (MQ=14)
                                                                  gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                            <  1:3341178/141‑1 (MQ=255)
                                                                      cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        <  1:2955295/141‑1 (MQ=255)
                                                                         aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt                                                                     >  1:3296864/1‑141 (MQ=255)
                                                                         aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATCCAGATAG‑‑‑CGAATAAGACGCAAATAGCGACTCAGATGCAGACAGAGAATCAGATTCAGATAGCGACTCAGACTCAGACACTgact                                                                     >  2:3828737/1‑139 (MQ=14)
                                                                            cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag                                                                  >  1:1702131/1‑141 (MQ=255)
                                                                               aCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagatt                                                               >  1:1520034/1‑141 (MQ=255)
                                                                                      ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGACTCGGACTCAGATAGCGACTCAGATTCAGAAAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACCGTgactcagatacaacgag                                                        >  2:2685471/1‑136 (MQ=14)
                                                                                          gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgac                                                    >  1:708162/1‑141 (MQ=255)
                                                                                           tgccAGATGGCCACTCAGCCTCAGACAGGGAGTCAGATTCAGCTAG‑‑‑AGATTCCGACTCAGATAGCGACTCAGCCTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgact                                                   <  2:176699/138‑1 (MQ=14)
                                                                                            tcgcgATACCGAATCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                  <  2:4004071/137‑1 (MQ=255)
                                                                                             tCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactca                                                 <  1:85228/141‑1 (MQ=255)
                                                                                               aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCCGACAGTGATTCAGATTCAGACAGCgacttaga                                               >  1:1553172/1‑141 (MQ=255)
                                                                                               aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTAAGACAGTGATTCAGCTTCAGACAGAgactcaaa                                               >  2:1036766/1‑139 (MQ=255)
                                                                                                  tAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactc                                            >  1:3853460/1‑141 (MQ=255)
                                                                                                   aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGGTTCAGATTCCGACAGCgactccgactca                                           >  1:1667109/1‑141 (MQ=255)
                                                                                                      gactcagactcaGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGAt                                        >  1:2869029/1‑141 (MQ=255)
                                                                                                        ctcagactcagaCAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAg                                      >  1:2918093/1‑141 (MQ=255)
                                                                                                             actcagacAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCCGATAGCGACTCAGACTCAGACCGTGATTCAGATCCAGACAACGACACACACACAAATaaacaca                                 >  2:4140412/1‑135 (MQ=17)
                                                                                                               tcagacAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCa                               >  1:271512/1‑141 (MQ=255)
                                                                                                               tcagacAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGAAAGCAACTCAGACTCAGATAGAGACTCa                               >  2:1181336/1‑141 (MQ=255)
                                                                                                               tcagacAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACACAGACAGCGACCCAGACTCAGATAGCGACTcc                               >  2:2152299/1‑140 (MQ=255)
                                                                                                                cagacAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAg                              >  1:2372299/1‑141 (MQ=255)
                                                                                                                 agacAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGa                             >  1:1740334/1‑141 (MQ=255)
                                                                                                                    cAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAtta                          >  1:3736260/1‑140 (MQ=255)
                                                                                                                    cAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCCACTCAGCTTc                          >  2:375357/1‑141 (MQ=255)
                                                                                                                     aGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCg                         >  1:2234565/1‑141 (MQ=255)
                                                                                                                      gCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCCGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACACAGATTCgg                        >  2:1256834/1‑141 (MQ=255)
                                                                                                                       cGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                       <  1:3161597/141‑1 (MQ=255)
                                                                                                                        gACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACACAGATAGAGACTCACATTCAGAc                      >  2:4386568/1‑141 (MQ=255)
                                                                                                                            cagattcagatAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACGCAGATAGCGACTCAGACTCGGACAGCg                  >  1:2998627/1‑141 (MQ=255)
                                                                                                                             agagtcagatAC‑‑‑CGATTGAGACTGTGATAGCGAATGAGACTCTGACAGCGAATCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCCGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGa                 <  2:1855671/141‑1 (MQ=14)
                                                                                                                               attcagatAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAgagaga               >  2:1235140/1‑139 (MQ=255)
                                                                                                                                ttcagatAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTc              <  1:3218664/141‑1 (MQ=255)
                                                                                                                                      tAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc        <  1:1267876/141‑1 (MQ=255)
                                                                                                                                      tAG‑‑‑CCATTCAGACACAGATAGCGACTCAGAGACAGACAGCGATTCAGACTCAGACAGCGACTCAGCCTCAGACAGACATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc        <  2:3457092/141‑1 (MQ=14)
                                                                                                                                      gaG‑‑‑CGAGTCGGACTCAGATAGCGTCTCTGAGTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc        <  2:53455/140‑1 (MQ=255)
                                                                                                                                       aG‑‑‑CGATTCGGACTCAGATAGCGACTAAGCTTCACAGCGCCATTCAGCCTCAGATAGCGACCCAGACTCAGACATCGAATCAGATACACACAGCAACTCAGACGCACATAGCGACCCAGACGAAGACAGAGACTCAGActct       >  2:2907369/1‑141 (MQ=9)
                                                                                                                                        g‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGGTTCGGACAGCGACTCAGACTCTg      >  1:279334/1‑141 (MQ=255)
                                                                                                                                        g‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg      <  1:1339919/141‑1 (MQ=255)
                                                                                                                                        g‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAAGCAGACATTTGTTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg      >  1:3451932/1‑141 (MQ=17)
                                                                                                                                        g‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTAAGAATCAGACAGTGAATCAGATTCAAACAGCGACTCAGACTCAGATAGAGAACCAGAATGTGACAACGACTCAGAcaatg      >  2:388477/1‑137 (MQ=14)
                                                                                                                                            cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGa     <  2:2336009/141‑1 (MQ=255)
                                                                                                                                            cGATTAGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGa     <  1:1819968/141‑1 (MQ=255)
                                                                                                                                               ttCTGACTCAGATGGCGGCTCCGACTCCGACAGAGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGATTCAGACGCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg  <  2:111600/141‑1 (MQ=17)
                                                                                                                                               |                                                                                                                                            
GACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAG  >  USA300TCH1516_ALE/622393‑622673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑