BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,852,974:1	+T	11.4%	coding (91/1419 nt)	yflS →	Putative malate transporter YflS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,852,974	1	.	T	11.4%	372.4 / 47.3	105	coding (91/1419 nt)	yflS	Putative malate transporter YflS
Reads supporting (aligned to +/- strand): ref base . (32/61); new base T (2/10); total (34/71)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 112 total aligned reads displayed.

TGGAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGTCTCT  >  USA300TCH1516_ALE/2852885‑2853063

tGGAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCtg‑t                                                                                           <  1:602242/91‑1 (MQ=255)

tGGAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCtg‑t                                                                                           <  1:602509/91‑1 (MQ=255)

 ggAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCtg‑tg                                                                                          >  1:295828/1‑91 (MQ=255)

 ggAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCtg‑tg                                                                                          >  1:295872/1‑91 (MQ=255)

 ggAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCtg‑tg                                                                                          >  1:296220/1‑91 (MQ=255)

 ggAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCtg‑tg                                                                                          <  1:1425355/91‑1 (MQ=255)

  gAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑Tgg                                                                                         >  1:2063948/1‑91 (MQ=255)

  gAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑Tgg                                                                                         >  1:2063902/1‑91 (MQ=255)

  gAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑Tgg                                                                                         >  1:3023468/1‑91 (MQ=255)

    aaaCACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGAt                                                                                       <  1:405414/91‑1 (MQ=255)

    aaaCACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGAt                                                                                       <  1:252360/91‑1 (MQ=255)

      acacGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATc                                                                                      <  1:2097461/91‑1 (MQ=255)

      acacGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATc                                                                                      <  1:769472/91‑1 (MQ=255)

      acacGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATc                                                                                      <  1:3740153/91‑1 (MQ=255)

      acacGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATcc                                                                                     >  1:1938330/1‑91 (MQ=255)

      acacGGTTAAAGATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATc                                                                                      <  1:3759608/91‑1 (MQ=255)

        acGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATCCa                                                                                    <  1:754294/91‑1 (MQ=255)

         cGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATCCac                                                                                   <  1:3671867/91‑2 (MQ=255)

         cGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATCCac                                                                                   <  1:4135238/91‑2 (MQ=255)

          ggTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATCCaca                                                                                  <  1:3091209/91‑3 (MQ=255)

          ggTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACa                                                                                 <  1:2547816/91‑1 (MQ=255)

           gTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATCCacag                                                                                 <  1:151689/91‑4 (MQ=255)

           gTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTGTTGGATCCacag                                                                                 <  1:2476403/91‑4 (MQ=255)

           gTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAg                                                                                >  1:1312216/1‑91 (MQ=255)

                 tatCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGt                                                                          >  1:570819/1‑91 (MQ=255)

                  atCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGta                                                                         <  1:2696790/91‑1 (MQ=255)

                  atCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGta                                                                         <  1:3938357/91‑1 (MQ=255)

                   tCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGtat                                                                        >  1:1594862/1‑91 (MQ=255)

                      tAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATg                                                                     <  1:3835945/91‑1 (MQ=255)

                       aaGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGt                                                                    <  1:2700041/91‑1 (MQ=255)

                         gTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTc                                                                  <  1:279492/91‑1 (MQ=255)

                          tttATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCg                                                                 <  1:2707031/91‑1 (MQ=255)

                          tttATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCg                                                                 >  1:2569864/1‑91 (MQ=255)

                           ttATACTCCTTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGc                                                                <  1:3732223/91‑1 (MQ=255)

                            tatgCTCCCGATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCa                                                               <  1:3727080/91‑1 (MQ=255)

                             ataCTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCaa                                                              <  1:2591277/91‑1 (MQ=255)

                             ataCTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCaa                                                              <  1:2594698/91‑1 (MQ=255)

                             ataCTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCaa                                                              <  1:147607/91‑1 (MQ=255)

                              taCTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAat                                                             <  1:1024871/91‑1 (MQ=255)

                              taCTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAat                                                             <  1:2967738/91‑1 (MQ=255)

                                   ccTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATtcg                                                       >  1:589574/1‑91 (MQ=255)

                                    cTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATtcgt                                                      <  1:2483592/91‑1 (MQ=255)

                                    cTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATtcgt                                                      <  1:2483616/91‑1 (MQ=255)

                                      aTCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATtcgtcg                                                    <  1:2105933/91‑1 (MQ=255)

                                       tCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGTATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTcgc                                                   <  1:707698/91‑1 (MQ=255)

                                       tCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTcgc                                                   <  1:1767476/91‑1 (MQ=255)

                                        cGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTcgcg                                                  <  1:3455772/91‑1 (MQ=255)

                                        cGTTGTAGGGCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTcgcg                                                  <  1:3985394/91‑1 (MQ=255)

                                         gTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGa                                                 <  1:2388742/91‑1 (MQ=255)

                                          ttGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGAc                                                <  1:3941282/91‑1 (MQ=255)

                                            gTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACaa                                              <  1:3396648/91‑1 (MQ=255)

                                            gTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACaa                                              <  1:3396522/91‑1 (MQ=255)

                                            gTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACaa                                              <  1:1597448/91‑1 (MQ=255)

                                            gTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACaa                                              <  1:3561015/91‑1 (MQ=255)

                                             tAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAATGATT‑GTCGCGAcgaa                                             <  1:1994546/91‑4 (MQ=255)

                                              aGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATc                                            <  1:1230453/91‑1 (MQ=255)

                                                 tctcCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCAtt                                         <  1:2323803/91‑1 (MQ=255)

                                                    ccTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCt                                      <  1:207477/91‑1 (MQ=255)

                                                     cTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCtt                                     <  1:1159806/91‑1 (MQ=255)

                                                      ttATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTg                                    >  1:2584897/1‑91 (MQ=255)

                                                        aTTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTa                                  >  1:727451/1‑91 (MQ=255)

                                                         tttGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTAt                                 >  1:2684611/1‑91 (MQ=255)

                                                            gggCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTac                              >  1:2944953/1‑91 (MQ=255)

                                                             ggCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTaca                             >  1:1139129/1‑91 (MQ=255)

                                                              gCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTacac                            <  1:3464817/91‑1 (MQ=255)

                                                              gCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTacac                            <  1:1285535/91‑1 (MQ=255)

                                                              gCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTacac                            <  1:3893567/91‑1 (MQ=255)

                                                              gCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTacac                            <  1:3893804/91‑1 (MQ=255)

                                                                 cTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAAc                         >  1:589889/1‑91 (MQ=255)

                                                                     cacCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGAt                     >  1:2597168/1‑91 (MQ=255)

                                                                     cacCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGAt                     >  1:2454591/1‑91 (MQ=255)

                                                                      acCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATg                    >  1:334189/1‑91 (MQ=255)

                                                                      acCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATg                    >  1:2813586/1‑91 (MQ=255)

                                                                      acCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATg                    <  1:1436216/91‑1 (MQ=255)

                                                                      acCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATg                    <  1:1436208/91‑1 (MQ=255)

                                                                      acCTGTTAAACCGGATGTTG‑TGGATCCAACAGCATTGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATg                    <  1:2697916/91‑1 (MQ=255)

                                                                       ccTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGc                   <  1:735141/91‑1 (MQ=255)

                                                                       ccTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGc                   <  1:813731/91‑1 (MQ=255)

                                                                       ccTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTACGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGc                   <  1:3900652/91‑1 (MQ=255)

                                                                        cTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGcc                  <  1:1335976/91‑1 (MQ=255)

                                                                        cTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGcc                  <  1:3960874/91‑1 (MQ=255)

                                                                         ttttAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCa                 >  1:3889044/1‑91 (MQ=255)

                                                                           ttAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAAt               <  1:413421/91‑1 (MQ=255)

                                                                            tAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAAtt              <  1:3938441/91‑1 (MQ=255)

                                                                             aaaCCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTg             >  1:1881333/1‑91 (MQ=255)

                                                                                ccGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTgggg          >  1:2147311/1‑91 (MQ=255)

                                                                                ccGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTgggg          >  1:2147435/1‑91 (MQ=255)

                                                                                 cGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGc         >  1:3419458/1‑91 (MQ=255)

                                                                                  ggATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGcc        >  1:2750025/1‑91 (MQ=255)

                                                                                  ggATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGcc        >  1:2931249/1‑91 (MQ=255)

                                                                                   gATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACCCCCGATGCCAATTGGGGCCg       <  1:639110/91‑1 (MQ=255)

                                                                                   gATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCg       <  1:638947/91‑1 (MQ=255)

                                                                                   gATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCg       <  1:1293340/91‑1 (MQ=255)

                                                                                    aTGCTGTTGGATCC‑ACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGt      >  1:3246877/1‑91 (MQ=255)

                                                                                    aTGCTGTTGGATCC‑ACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGt      >  1:3810122/1‑91 (MQ=255)

                                                                                     tGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGtc     <  1:3022914/91‑1 (MQ=255)

                                                                                     tGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGtc     <  1:3244637/91‑1 (MQ=255)

                                                                                      gCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGtct    <  1:1207151/91‑1 (MQ=255)

                                                                                       cTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGtctc   >  1:2664103/1‑91 (MQ=255)

                                                                                        tg‑tgGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGTctct  <  1:2294104/91‑1 (MQ=255)

TGGAAAACACGGTTAAATATCGTAAGTTTATACTCCCTATCGTTGTAGGTCTCCTTATTTGGGCACTTACACCTTTTAAACCGGATGCTG‑TGGATCCAACAGCATGGTATATGTTCGCAAT‑ATTCGTCGCGACAATCATTGCTTGTATTACACAACCGATGCCAATTGGGGCCGTCTCT  >  USA300TCH1516_ALE/2852885‑2853063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`