BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	806,425	C→A	6.0%	M126I (ATG→ATT)	ribN ←	Riboflavin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	806,425	0	C	A	6.0%	412.8 / 12.2	118	M126I (ATG→ATT)	ribN	Riboflavin transporter
Reads supporting (aligned to +/- strand): ref base C (59/51); new base A (4/3); total (63/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 118 total aligned reads displayed.

ATACCTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTAATG  >  USA300TCH1516_ALE/806336‑806513

aTACCTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCa                                                                                         >  1:906536/1‑91 (MQ=255)

aTACCTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCa                                                                                         >  1:906403/1‑91 (MQ=255)

  aCCTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCAtt                                                                                       >  1:1513668/1‑91 (MQ=255)

  aCCTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCAtt                                                                                       <  1:285057/91‑1 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTg                                                                                      <  1:2568456/91‑1 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTg                                                                                      >  1:2757069/1‑91 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTg                                                                                      <  1:1268686/91‑1 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTg                                                                                      <  1:1268948/91‑1 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTg                                                                                      >  1:586584/1‑91 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATGATGATTg                                                                                      <  1:3276868/91‑1 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATGATAATTg                                                                                      <  1:53608/91‑1 (MQ=255)

   ccTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATGATAATTg                                                                                      <  1:53435/91‑1 (MQ=255)

    cTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGc                                                                                     >  1:521699/1‑91 (MQ=255)

    cTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGc                                                                                     >  1:68921/1‑91 (MQ=255)

       agaATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGt                                                                                  <  1:1548665/91‑1 (MQ=255)

       agaATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGt                                                                                  >  1:3615193/1‑91 (MQ=255)

       agaATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGt                                                                                  >  1:3614283/1‑91 (MQ=255)

           tAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAAtt                                                                              <  1:3373410/91‑1 (MQ=255)

           tAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAAtt                                                                              <  1:1836693/91‑1 (MQ=255)

             aGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTg                                                                            >  1:3067443/1‑91 (MQ=255)

              gCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGa                                                                           >  1:1484282/1‑91 (MQ=255)

              gCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGa                                                                           >  1:2281715/1‑91 (MQ=255)

                      atgatgGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCt                                                                   <  1:2932600/91‑1 (MQ=255)

                      atgatgGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCt                                                                   <  1:3364579/91‑1 (MQ=255)

                       tgatgGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCtt                                                                  >  1:2497059/1‑91 (MQ=255)

                          tgGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTAcc                                                               >  1:8809/1‑91 (MQ=255)

                            gAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCtt                                                             <  1:1954003/91‑1 (MQ=255)

                             aaTCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCttt                                                            >  1:2539776/1‑91 (MQ=255)

                               tCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTc                                                          <  1:1710556/91‑1 (MQ=255)

                               tCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTc                                                          >  1:3963288/1‑91 (MQ=255)

                                 aTAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCAt                                                        <  1:4090193/91‑1 (MQ=255)

                                 aTAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCAt                                                        <  1:2841498/91‑1 (MQ=255)

                                 aTAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCAt                                                        <  1:1074571/91‑1 (MQ=255)

                                  tAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATg                                                       >  1:1587501/1‑91 (MQ=255)

                                    gatgatGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTa                                                     >  1:2129162/1‑91 (MQ=255)

                                     atgatgAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTaa                                                    >  1:2852353/1‑91 (MQ=255)

                                     atgatgAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTaa                                                    >  1:2848744/1‑91 (MQ=255)

                                      tgatgaAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAg                                                   >  1:679679/1‑91 (MQ=255)

                                       gatgaAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGa                                                  >  1:330386/1‑91 (MQ=255)

                                         tgaAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGaaa                                                <  1:939171/91‑1 (MQ=255)

                                           aaaaTTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAAc                                              <  1:742395/91‑1 (MQ=255)

                                           aaaaTTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAAc                                              <  1:740095/91‑1 (MQ=255)

                                            aaaTTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACa                                             >  1:409847/1‑91 (MQ=255)

                                             aaTTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACaa                                            <  1:4039169/91‑1 (MQ=255)

                                             aaTTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACaa                                            <  1:1446328/91‑1 (MQ=255)

                                               ttCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATg                                          <  1:2181463/91‑1 (MQ=255)

                                               ttCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATg                                          <  1:2181517/91‑1 (MQ=255)

                                                tCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGc                                         <  1:1407938/91‑1 (MQ=255)

                                                 cAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCt                                        >  1:1962343/1‑91 (MQ=255)

                                                    gTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTtaa                                     >  1:456274/1‑91 (MQ=255)

                                                    gTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTtaa                                     >  1:456207/1‑91 (MQ=255)

                                                     tttCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTtaat                                    <  1:56718/91‑1 (MQ=255)

                                                       tCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTtaataa                                  <  1:2841781/91‑1 (MQ=255)

                                                        cACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATaaa                                 <  1:1681778/91‑1 (MQ=255)

                                                        cACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATaaa                                 >  1:2026215/1‑91 (MQ=255)

                                                         aCTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATaaaa                                >  1:1194953/1‑91 (MQ=255)

                                                             ttAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGt                            >  1:2523678/1‑91 (MQ=255)

                                                             ttAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGt                            >  1:2186678/1‑91 (MQ=255)

                                                               aaaaGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCATACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTcc                          >  1:401028/1‑91 (MQ=255)

                                                               aaaaGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCATACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTcc                          >  1:401014/1‑91 (MQ=255)

                                                                aaaGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCa                         >  1:789636/1‑91 (MQ=255)

                                                                 aaGCATACCTAAGATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAg                        >  1:3190783/1‑91 (MQ=255)

                                                                 aaGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAg                        >  1:3909893/1‑91 (MQ=255)

                                                                 aaGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAg                        >  1:4052889/1‑91 (MQ=255)

                                                                  aGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGa                       >  1:1359099/1‑91 (MQ=255)

                                                                  aGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGa                       <  1:4087763/91‑1 (MQ=255)

                                                                   gCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGaa                      >  1:2539847/1‑91 (MQ=255)

                                                                    cATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGaaa                     >  1:2806660/1‑91 (MQ=255)

                                                                    cATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGaaa                     >  1:934813/1‑91 (MQ=255)

                                                                     aTACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAg                    >  1:3112029/1‑91 (MQ=255)

                                                                      tACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAgg                   <  1:1477462/91‑1 (MQ=255)

                                                                      tACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAgg                   >  1:3135228/1‑91 (MQ=255)

                                                                      tACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAgg                   <  1:1477701/91‑1 (MQ=255)

                                                                        ccTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGAt                 <  1:1312428/91‑1 (MQ=255)

                                                                         cTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGAtt                <  1:3497997/91‑1 (MQ=255)

                                                                         cTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGAtt                <  1:2381406/91‑1 (MQ=255)

                                                                          tAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTg               >  1:1266197/1‑91 (MQ=255)

                                                                             aaTAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCNTGTAAGAAAACNATGCNTAATAAAATCGTCCAGAAAGGATTGAGt            >  1:1135607/1‑91 (MQ=255)

                                                                             aaTAACAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGt            <  1:3074524/91‑1 (MQ=255)

                                                                              acaGCAATGATAATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGtt           >  1:182907/3‑91 (MQ=255)

                                                                              aTAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTGTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGtt           <  1:2697575/91‑1 (MQ=255)

                                                                               caGCAATGATAATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGttt          >  1:1969264/2‑91 (MQ=255)

                                                                                aGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTc         >  1:380531/1‑91 (MQ=255)

                                                                                aGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTc         >  1:3044358/1‑91 (MQ=255)

                                                                                aGCAATGATAATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTc         >  1:2440290/1‑91 (MQ=255)

                                                                                aGCAATGATAATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTc         >  1:2440289/1‑91 (MQ=255)

                                                                                 gCAATTATCATTGCTGTAATTTGATACTTCCTTGCCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCa        <  1:1248922/91‑1 (MQ=255)

                                                                                 gCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCa        >  1:500410/1‑91 (MQ=255)

                                                                                 gCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCa        <  1:2866417/91‑1 (MQ=255)

                                                                                 gCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCa        <  1:2288946/91‑1 (MQ=255)

                                                                                 gCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCa        <  1:1248854/91‑1 (MQ=255)

                                                                                  cAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCGGAAAGGATTGAGTTTCAt       >  1:2926657/1‑91 (MQ=255)

                                                                                  cAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCAt       >  1:3638812/1‑91 (MQ=255)

                                                                                  cAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCAt       >  1:3640172/1‑91 (MQ=255)

                                                                                   aaTTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCAtt      >  1:2145140/1‑91 (MQ=255)

                                                                                    aTTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTa     >  1:1675223/1‑91 (MQ=255)

                                                                                     ttATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTaa    <  1:156167/91‑1 (MQ=255)

                                                                                     ttATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTaa    <  1:4020000/91‑1 (MQ=255)

                                                                                       aTCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTAATg  >  1:246518/1‑91 (MQ=255)

                                                                                       aTCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTAATg  >  1:246952/1‑91 (MQ=255)

ATACCTGAGAATAAGCCAGCTAATGATGGAATCATAGATGATGAAAATTCAGGTTTCACTATTAAAAGCATACCTAAAATAGCAATTATCATTGCTGTAATTTGATACTTCCTTACCTTTTCATGTAAGAAAACAATGCTTAATAAAATCGTCCAGAAAGGATTGAGTTTCATTAATG  >  USA300TCH1516_ALE/806336‑806513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`