BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,019,746	G→T	6.5%	intergenic (+68/+45)	USA300HOU_RS05030 → / ← ltaA	Putative phosphoesterase/putative glycolipid permease LtaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,019,746	0	G	T	6.5%	381.1 / 14.3	108	intergenic (+68/+45)	USA300HOU_RS05030/ltaA	Putative phosphoesterase/putative glycolipid permease LtaA
Reads supporting (aligned to +/- strand): ref base G (46/55); new base T (2/5); total (48/60)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 113 total aligned reads displayed.

TTGAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAT  >  USA300TCH1516_ALE/1019656‑1019836

ttGAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATg                                                                                            <  1:1316065/91‑1 (MQ=255)

ttGAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATg                                                                                            <  1:1327077/91‑1 (MQ=255)

ttGAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATg                                                                                            <  1:2856658/91‑1 (MQ=255)

  gAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGta                                                                                          >  1:1310507/1‑91 (MQ=255)

   aaaCGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaa                                                                                         <  1:2143470/91‑1 (MQ=255)

   aaaCGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaa                                                                                         >  1:896014/1‑91 (MQ=255)

   aaaCGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaa                                                                                         <  1:1429962/91‑1 (MQ=255)

    aaCGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaat                                                                                        <  1:627881/91‑1 (MQ=255)

        tttAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATaaaa                                                                                    >  1:1409709/1‑91 (MQ=255)

         ttAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATaaaaa                                                                                   >  1:3951232/1‑91 (MQ=255)

           aaaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATg                                                                                 >  1:3951332/1‑91 (MQ=255)

           aaaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATg                                                                                 >  1:1588063/1‑91 (MQ=255)

            aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAACTGa                                                                                >  1:3348106/1‑91 (MQ=255)

            aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGa                                                                                >  1:1785132/1‑91 (MQ=255)

            aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGa                                                                                >  1:201005/1‑91 (MQ=255)

            aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGa                                                                                >  1:53660/1‑91 (MQ=255)

            aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGa                                                                                <  1:2482623/91‑1 (MQ=25)

            aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGa                                                                                <  1:801153/91‑1 (MQ=25)

             aTTAGCTTGAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAGAATGAc                                                                               <  1:2979366/91‑1 (MQ=255)

             aTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGAc                                                                               <  1:2979097/91‑1 (MQ=255)

             aTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGAc                                                                               <  1:1823466/91‑1 (MQ=25)

              ttAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGAcc                                                                              <  1:50084/91‑1 (MQ=255)

              ttAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGAcc                                                                              <  1:442185/91‑1 (MQ=255)

               tAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCa                                                                             <  1:681504/91‑1 (MQ=255)

                aGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAt                                                                            >  1:2495373/1‑91 (MQ=255)

                aGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAt                                                                            >  1:3917763/1‑91 (MQ=255)

                 gCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAtt                                                                           <  1:3445248/91‑1 (MQ=255)

                   ttAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAtttt                                                                         <  1:762308/91‑1 (MQ=255)

                    tAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGACCATTTTc                                                                        <  1:1097673/91‑1 (MQ=21)

                     aaGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCt                                                                       <  1:2203598/91‑1 (MQ=255)

                      aGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCtt                                                                      <  1:3753350/91‑1 (MQ=255)

                         aaCATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTAt                                                                   <  1:427222/91‑1 (MQ=255)

                          aCATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTAtt                                                                  >  1:1945682/1‑91 (MQ=255)

                              aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              >  1:3660615/1‑91 (MQ=255)

                              aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              >  1:3660475/1‑91 (MQ=255)

                              aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              <  1:572759/91‑1 (MQ=255)

                              aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              >  1:2730740/1‑91 (MQ=255)

                              aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              <  1:699086/91‑1 (MQ=255)

                                     ttGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGt                                                       >  1:534037/1‑91 (MQ=255)

                                     ttGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGt                                                       >  1:2394238/1‑91 (MQ=255)

                                      tGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGtt                                                      <  1:2695993/91‑1 (MQ=255)

                                        ttAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGtttt                                                    <  1:462384/91‑1 (MQ=255)

                                            tCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAAcc                                                <  1:948951/91‑1 (MQ=255)

                                             cGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACct                                               >  1:107206/1‑91 (MQ=255)

                                              gtagtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACctt                                              >  1:2588819/1‑91 (MQ=255)

                                                agtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttac                                            >  1:1055217/1‑91 (MQ=255)

                                                 gtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttact                                           <  1:250426/91‑1 (MQ=255)

                                                  taTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttactt                                          <  1:2834288/91‑1 (MQ=255)

                                                  taTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttactt                                          <  1:2268631/91‑1 (MQ=255)

                                                   aTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttactta                                         >  1:1727369/1‑91 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2241355/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:573036/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        >  1:1771147/1‑91 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2241381/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2127656/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2240903/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2241777/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2240924/91‑1 (MQ=255)

                                                    tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACGTAg                                        >  1:3348875/1‑91 (MQ=255)

                                                       ttGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCtt                                     <  1:20328/91‑1 (MQ=255)

                                                        tGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCttt                                    <  1:2895786/91‑1 (MQ=255)

                                                          aaTTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCttttt                                  <  1:1494713/91‑1 (MQ=255)

                                                            ttAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTtct                                <  1:3601414/91‑1 (MQ=255)

                                                               ataaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTa                             >  1:442797/1‑91 (MQ=255)

                                                               ataaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTa                             >  1:566817/1‑91 (MQ=255)

                                                               ataaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTa                             >  1:2624734/1‑91 (MQ=255)

                                                                taaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTAt                            >  1:1496648/1‑91 (MQ=255)

                                                                  aGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTAttt                          <  1:1797640/91‑1 (MQ=255)

                                                                    aaaaTGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTAc                        >  1:48208/1‑91 (MQ=255)

                                                                       aTGGTCATTTTTATTACATTCAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACATTACTTAGCTTTTTCTCTATTTACtat                     >  1:1405176/1‑91 (MQ=21)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2693806/91‑1 (MQ=255)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:1360801/91‑1 (MQ=255)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2570828/91‑1 (MQ=255)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2765330/91‑1 (MQ=255)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    >  1:2447118/1‑91 (MQ=255)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2387617/91‑1 (MQ=255)

                                                                        tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2693768/91‑1 (MQ=255)

                                                                         ggTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaa                   >  1:2453334/1‑91 (MQ=255)

                                                                         ggTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaa                   >  1:1772389/1‑91 (MQ=255)

                                                                         ggTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaa                   <  1:1208066/91‑1 (MQ=255)

                                                                          gTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  <  1:1931258/91‑1 (MQ=255)

                                                                          gTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  >  1:2668510/1‑91 (MQ=255)

                                                                          gTCATTTTTATTACATTCAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  >  1:3956062/1‑91 (MQ=25)

                                                                             aTTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTa               >  1:2996305/1‑91 (MQ=255)

                                                                               ttttATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGc             >  1:3819121/1‑91 (MQ=255)

                                                                                 ttATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCtt           >  1:383985/1‑91 (MQ=255)

                                                                                   aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         <  1:493152/91‑1 (MQ=255)

                                                                                   aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         <  1:1620521/91‑1 (MQ=255)

                                                                                   aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         >  1:1962707/1‑91 (MQ=255)

                                                                                   aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         >  1:2267417/1‑91 (MQ=255)

                                                                                    ttGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCa        >  1:2948920/1‑91 (MQ=255)

                                                                                    ttGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCa        <  1:906441/91‑1 (MQ=255)

                                                                                     tGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTATTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCAt       <  1:3493454/91‑1 (MQ=255)

                                                                                     tGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCAt       <  1:83215/91‑1 (MQ=255)

                                                                                      gAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTGGCTTTTTCTCTATTTACTATAAATTCGCTTCCAtc      >  1:1536411/1‑90 (MQ=255)

                                                                                       aaTGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaa     <  1:1598574/91‑1 (MQ=255)

                                                                                       aaTGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaa     <  1:439771/91‑1 (MQ=255)

                                                                                       aaTGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaa     <  1:3697660/91‑1 (MQ=255)

                                                                                         tGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaaga   >  1:1414909/1‑89 (MQ=255)

                                                                                          gTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAt  <  1:845965/91‑1 (MQ=255)

TTGAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAT  >  USA300TCH1516_ALE/1019656‑1019836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`