BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,019,747	T→C	6.7%	intergenic (+69/+44)	USA300HOU_RS05030 → / ← ltaA	Putative phosphoesterase/putative glycolipid permease LtaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,019,747	0	T	C	6.7%	341.2 / 11.2	104	intergenic (+69/+44)	USA300HOU_RS05030/ltaA	Putative phosphoesterase/putative glycolipid permease LtaA
Reads supporting (aligned to +/- strand): ref base T (43/54); new base C (2/5); total (45/59)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 110 total aligned reads displayed.

GAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAT  >  USA300TCH1516_ALE/1019658‑1019836

gAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGta                                                                                          >  1:1310507/1‑91 (MQ=255)

 aaaCGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaa                                                                                         <  1:2143470/91‑1 (MQ=255)

 aaaCGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaa                                                                                         >  1:896014/1‑91 (MQ=255)

    cGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGtaataa                                                                                      >  1:73495/1‑91 (MQ=255)

      tttAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATaaaa                                                                                    >  1:1409709/1‑91 (MQ=255)

       ttAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATaaaaa                                                                                   >  1:3951232/1‑91 (MQ=255)

         aaaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATg                                                                                 >  1:3951332/1‑91 (MQ=255)

         aaaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATg                                                                                 >  1:1588063/1‑91 (MQ=255)

          aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAACTGa                                                                                >  1:3348106/1‑91 (MQ=255)

          aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGa                                                                                >  1:201005/1‑91 (MQ=255)

          aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGa                                                                                >  1:53660/1‑91 (MQ=255)

          aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGa                                                                                <  1:2482623/91‑1 (MQ=25)

          aaTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGa                                                                                <  1:801153/91‑1 (MQ=25)

           aTTAGCTTGAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAGAATGAc                                                                               <  1:2979366/91‑1 (MQ=255)

           aTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGAc                                                                               <  1:2979097/91‑1 (MQ=255)

           aTTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGAc                                                                               <  1:1823466/91‑1 (MQ=25)

            ttAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGAcc                                                                              <  1:50084/91‑1 (MQ=255)

            ttAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGAcc                                                                              <  1:442185/91‑1 (MQ=255)

             tAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCa                                                                             <  1:681504/91‑1 (MQ=255)

              aGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAt                                                                            >  1:3917763/1‑91 (MQ=255)

              aGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAt                                                                            >  1:2495373/1‑91 (MQ=255)

               gCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAtt                                                                           <  1:3445248/91‑1 (MQ=255)

               gCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGACCAtt                                                                           <  1:2350768/91‑1 (MQ=21)

                 ttAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCAtttt                                                                         <  1:762308/91‑1 (MQ=255)

                  tAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTACATTCAATAAAAATGACCATTTTc                                                                        <  1:1097673/91‑1 (MQ=21)

                   aaGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCt                                                                       <  1:2203598/91‑1 (MQ=255)

                    aGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCtt                                                                      <  1:3753283/91‑1 (MQ=255)

                       aaCATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTAt                                                                   <  1:427222/91‑1 (MQ=255)

                        aCATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTAtt                                                                  >  1:1945682/1‑91 (MQ=255)

                            aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              <  1:699086/91‑1 (MQ=255)

                            aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              >  1:2730740/1‑91 (MQ=255)

                            aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              <  1:572759/91‑1 (MQ=255)

                            aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              >  1:3660475/1‑91 (MQ=255)

                            aataGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTaa                                                              >  1:3660615/1‑91 (MQ=255)

                                   ttGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGt                                                       >  1:534037/1‑91 (MQ=255)

                                   ttGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGt                                                       >  1:533783/1‑91 (MQ=255)

                                    tGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGtt                                                      <  1:2695993/91‑1 (MQ=255)

                                      ttAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGtttt                                                    <  1:462384/91‑1 (MQ=255)

                                          tCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAAcc                                                <  1:948951/91‑1 (MQ=255)

                                           cGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACct                                               >  1:107206/1‑91 (MQ=255)

                                            gtagtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACctt                                              >  1:2582982/1‑91 (MQ=255)

                                            gtagtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACctt                                              >  1:2588819/1‑91 (MQ=255)

                                              agtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttac                                            >  1:1055217/1‑91 (MQ=255)

                                               gtaTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttact                                           <  1:250426/91‑1 (MQ=255)

                                                taTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttactt                                          <  1:2268631/91‑1 (MQ=255)

                                                taTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttactt                                          <  1:2834288/91‑1 (MQ=255)

                                                 aTGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACcttactta                                         >  1:1727369/1‑91 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2127656/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        >  1:1771147/1‑91 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2240924/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2240903/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2241355/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2241777/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:2241381/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAg                                        <  1:573036/91‑1 (MQ=255)

                                                  tGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACGTAg                                        >  1:3348875/1‑91 (MQ=255)

                                                     ttGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCtt                                     <  1:20328/91‑1 (MQ=255)

                                                      tGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCttt                                    <  1:2895786/91‑1 (MQ=255)

                                                        aaTTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCttttt                                  <  1:1494713/91‑1 (MQ=255)

                                                          ttAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTtct                                <  1:3601414/91‑1 (MQ=255)

                                                             ataaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTa                             >  1:566817/1‑91 (MQ=255)

                                                             ataaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTa                             >  1:2624734/1‑91 (MQ=255)

                                                              taaGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTAt                            >  1:1496648/1‑91 (MQ=255)

                                                                aGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTAttt                          <  1:1797640/91‑1 (MQ=255)

                                                                  aaaaTGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTAc                        >  1:48208/1‑91 (MQ=255)

                                                                     aTGGTCATTTTTATTACATTCAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACATTACTTAGCTTTTTCTCTATTTACtat                     >  1:1405176/1‑91 (MQ=21)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    >  1:2447118/1‑91 (MQ=255)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:1360801/91‑1 (MQ=255)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2765330/91‑1 (MQ=255)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2764778/91‑1 (MQ=255)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2693806/91‑1 (MQ=255)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2570828/91‑1 (MQ=255)

                                                                      tGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACtata                    <  1:2387617/91‑1 (MQ=255)

                                                                       ggTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaa                   <  1:1208066/91‑1 (MQ=255)

                                                                       ggTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaa                   >  1:2453334/1‑91 (MQ=255)

                                                                       ggTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaa                   >  1:1772389/1‑91 (MQ=255)

                                                                        gTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  >  1:4077805/1‑91 (MQ=255)

                                                                        gTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  >  1:2668510/1‑91 (MQ=255)

                                                                        gTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  <  1:1931258/91‑1 (MQ=255)

                                                                        gTCATTTTTATTACATTCAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATaaa                  >  1:3956062/1‑91 (MQ=25)

                                                                         tCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAg                 <  1:1854902/91‑1 (MQ=255)

                                                                             ttttATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGc             >  1:3819121/1‑91 (MQ=255)

                                                                              tttATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCt            <  1:3336474/91‑1 (MQ=255)

                                                                               ttATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCtt           >  1:383985/1‑91 (MQ=255)

                                                                                 aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         <  1:493152/91‑1 (MQ=255)

                                                                                 aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         <  1:1620521/91‑1 (MQ=255)

                                                                                 aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         >  1:2267417/1‑91 (MQ=255)

                                                                                 aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         >  1:1962707/1‑91 (MQ=255)

                                                                                 aTTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTcc         <  1:136968/91‑1 (MQ=255)

                                                                                  ttGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCa        <  1:906441/91‑1 (MQ=255)

                                                                                  ttGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCa        >  1:2948920/1‑91 (MQ=255)

                                                                                   tGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTATTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCAt       <  1:3493454/91‑1 (MQ=255)

                                                                                   tGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCAt       <  1:83215/91‑1 (MQ=255)

                                                                                    gAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTGGCTTTTTCTCTATTTACTATAAATTCGCTTCCAtc      >  1:1536411/1‑90 (MQ=255)

                                                                                     aaTGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaa     <  1:3697660/91‑1 (MQ=255)

                                                                                     aaTGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaa     <  1:1598574/91‑1 (MQ=255)

                                                                                     aaTGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaa     <  1:439771/91‑1 (MQ=255)

                                                                                       tGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATaaga   >  1:1414909/1‑89 (MQ=255)

                                                                                        gTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAt  <  1:845965/91‑1 (MQ=255)

                                                                                        gTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAt  <  1:159239/91‑1 (MQ=255)

GAAACGTTTAAATTAGCTTAAGTAACATAATAGTATTGTTAATCGTAGTATGTTTGAATTAATAAGAAAATGGTCATTTTTATTGAATGTAATAAAAATGACCATTTTCTTTATTTTAAAATACGTTTTAACCTTACTTAGCTTTTTCTCTATTTACTATAAAGTAGCTTCCATAAAAT  >  USA300TCH1516_ALE/1019658‑1019836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`