BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,476,681	T→A	11.5%	H8448L (CAT→CTT)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,476,681	0	T	A	11.5%	329.4 / 27.8	104	H8448L (CAT→CTT)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base T (50/42); new base A (2/10); total (52/52)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCT  >  USA300TCH1516_ALE/1476592‑1476770

cATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATg                                                                                          >  1:1296901/1‑91 (MQ=255)

cATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATg                                                                                          >  1:1297367/1‑91 (MQ=255)

 aTAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGc                                                                                         <  1:483158/91‑1 (MQ=255)

 aTAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGc                                                                                         <  1:555847/91‑1 (MQ=255)

 aTAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGc                                                                                         <  1:140997/91‑1 (MQ=255)

  tAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCa                                                                                        <  1:4015906/91‑1 (MQ=255)

   aGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCAt                                                                                       <  1:2149542/91‑1 (MQ=255)

    gTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCAtt                                                                                      <  1:3122502/91‑1 (MQ=255)

    gTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCAtt                                                                                      <  1:3122654/91‑1 (MQ=255)

     ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                     <  1:1647215/91‑1 (MQ=255)

     ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                     <  1:1647661/91‑1 (MQ=255)

     ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                     <  1:3666528/91‑1 (MQ=255)

     ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                     <  1:2896496/91‑1 (MQ=255)

      tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGc                                                                                    >  1:4121192/1‑91 (MQ=255)

      tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                    <  1:3117676/91‑1 (MQ=255)

      tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                    <  1:2392483/91‑1 (MQ=255)

      tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                    <  1:2392413/91‑1 (MQ=255)

      tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                    <  1:2139859/91‑1 (MQ=255)

       ttgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGca                                                                                   <  1:3778961/91‑2 (MQ=255)

        tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                  <  1:671478/91‑3 (MQ=255)

        tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                  <  1:168795/91‑3 (MQ=255)

        tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                  <  1:3086940/91‑3 (MQ=255)

        tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                  <  1:1270419/91‑3 (MQ=255)

          ttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTgg                                                                                >  1:2183550/1‑91 (MQ=255)

            gCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGtt                                                                              >  1:1582090/1‑91 (MQ=255)

             ctctGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTa                                                                             >  1:1338087/1‑91 (MQ=255)

             ctctGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTa                                                                             <  1:3584235/91‑1 (MQ=255)

                tGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCCTAAACCATGCATTGCTTGGTTAAGc                                                                          >  1:3874619/1‑91 (MQ=255)

                 ggTTGACCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCg                                                                         >  1:3221400/1‑91 (MQ=255)

                  gTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATACTTTCTCTTAAGCCATGCATTGCTTGGTTAAGCGt                                                                        <  1:2411110/91‑1 (MQ=255)

                    tGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTTGTTAAGCGTAt                                                                      <  1:2295196/91‑1 (MQ=255)

                     gATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTAtt                                                                     >  1:2516946/1‑91 (MQ=255)

                      aTCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTc                                                                    >  1:2546713/1‑91 (MQ=255)

                      aTCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTc                                                                    >  1:3715099/1‑91 (MQ=255)

                       tCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCg                                                                   <  1:1695953/91‑1 (MQ=255)

                         ttCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCa                                                                 >  1:2386901/1‑91 (MQ=255)

                           cGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAtt                                                               <  1:60221/91‑1 (MQ=255)

                            gTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAttt                                                              <  1:1094694/91‑1 (MQ=255)

                            gTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAttt                                                              <  1:1961254/91‑1 (MQ=255)

                            gTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAttt                                                              <  1:1371317/91‑1 (MQ=255)

                              tGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTg                                                            >  1:3108345/1‑91 (MQ=255)

                                atatatTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGtt                                                          <  1:3251751/91‑1 (MQ=255)

                                  atatTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCa                                                        >  1:761247/1‑91 (MQ=255)

                                     tttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAtt                                                     >  1:237627/1‑91 (MQ=255)

                                     tttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAtt                                                     >  1:528385/1‑91 (MQ=255)

                                     tttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAtt                                                     >  1:1307012/1‑91 (MQ=255)

                                      ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                    >  1:1376190/1‑91 (MQ=255)

                                      ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                    >  1:2956811/1‑91 (MQ=255)

                                      ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGATTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                    >  1:112892/1‑91 (MQ=255)

                                       tgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtg                                                   >  1:3299731/1‑91 (MQ=255)

                                       tgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCGTGGTTAAGCGTATTCGCATTTTGTTCAATTtg                                                   >  1:1964803/1‑91 (MQ=255)

                                        gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                  <  1:3967567/91‑1 (MQ=255)

                                        gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                  <  1:2289273/91‑1 (MQ=255)

                                        gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                  <  1:2289939/91‑1 (MQ=255)

                                        gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                  <  1:2289767/91‑1 (MQ=255)

                                         ctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTATCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtgat                                                 >  1:3507254/1‑91 (MQ=255)

                                             ttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAAc                                             >  1:12133/1‑91 (MQ=255)

                                               gctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTg                                           <  1:314210/91‑1 (MQ=255)

                                                  ttAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAg                                        >  1:4110281/1‑91 (MQ=255)

                                                   tAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGt                                       >  1:1893325/1‑91 (MQ=255)

                                                     gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                     >  1:4104438/1‑91 (MQ=255)

                                                     gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                     >  1:1196197/1‑91 (MQ=255)

                                                     gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                     >  1:269403/1‑91 (MQ=255)

                                                      ttGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAc                                    <  1:3809082/91‑1 (MQ=255)

                                                       tgctgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACt                                   <  1:2045402/91‑1 (MQ=255)

                                                       tgctgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACt                                   <  1:705479/91‑1 (MQ=255)

                                                          tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTaaa                                >  1:1507819/1‑91 (MQ=255)

                                                          tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTaaa                                <  1:3619678/91‑1 (MQ=255)

                                                          tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGCTACTaaa                                >  1:2067416/1‑91 (MQ=255)

                                                           gcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAg                               >  1:256648/1‑91 (MQ=255)

                                                            cGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGt                              >  1:1673477/1‑91 (MQ=255)

                                                             gTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGtt                             >  1:486372/1‑91 (MQ=255)

                                                                  cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAGTAAAGTTGCAGc                        <  1:3204838/91‑1 (MQ=255)

                                                                  cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAGTAAAGTTGCAGc                        <  1:3205405/91‑1 (MQ=255)

                                                                  cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGc                        <  1:83780/91‑1 (MQ=255)

                                                                  cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCACATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGc                        >  1:2400368/1‑91 (MQ=255)

                                                                   ttGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCt                       >  1:2205723/1‑91 (MQ=255)

                                                                    tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                      <  1:3094721/91‑1 (MQ=255)

                                                                    tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                      >  1:3795181/1‑91 (MQ=255)

                                                                    tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                      >  1:3795335/1‑91 (MQ=255)

                                                                    tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                      >  1:1366848/1‑91 (MQ=255)

                                                                    tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                      <  1:262868/91‑1 (MQ=255)

                                                                     gAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGt                     <  1:3529774/91‑1 (MQ=255)

                                                                        tGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCGTTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAAc                  <  1:4139667/91‑1 (MQ=255)

                                                                        tGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTACAGCTGCAAc                  <  1:1919575/91‑1 (MQ=255)

                                                                          ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACtt                >  1:1780026/1‑91 (MQ=255)

                                                                          ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACtt                >  1:1937216/1‑91 (MQ=255)

                                                                          ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACtt                >  1:1939007/1‑91 (MQ=255)

                                                                          ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTACAGCTGCAACtt                <  1:3945801/91‑1 (MQ=255)

                                                                           tctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTg               >  1:1528954/1‑91 (MQ=255)

                                                                            ctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTgg              >  1:424859/1‑91 (MQ=255)

                                                                              gTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTc            >  1:1599871/1‑91 (MQ=255)

                                                                                cTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtt          <  1:2724256/91‑1 (MQ=255)

                                                                                cTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATGTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtt          >  1:3485959/1‑91 (MQ=255)

                                                                                  tAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtttt        <  1:2838982/91‑1 (MQ=255)

                                                                                   aaaCCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTa       >  1:1554437/1‑91 (MQ=255)

                                                                                   aaaCCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTa       >  1:807950/1‑91 (MQ=255)

                                                                                     aCCAAGCAATGCATGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAAt     >  1:3053742/1‑91 (MQ=255)

                                                                                     aCCAAGCAATGCATGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAAt     >  1:3056632/1‑91 (MQ=255)

                                                                                        aTGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCt  <  1:1025084/91‑1 (MQ=255)

CATAGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCT  >  USA300TCH1516_ALE/1476592‑1476770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`