BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,476,685	T→A	12.2%	M8447L (ATG→TTG)	ebh_1 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,476,685	0	T	A	12.2%	303.8 / 29.3	98	M8447L (ATG→TTG)	ebh_1	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand): ref base T (50/36); new base A (3/9); total (53/45)
Fisher's exact test for biased strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 101 total aligned reads displayed.

AGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTT  >  USA300TCH1516_ALE/1476595‑1476774

aGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCAt                                                                                           <  1:2149542/91‑1 (MQ=255)

 gTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCAtt                                                                                          <  1:3122502/91‑1 (MQ=255)

 gTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCAtt                                                                                          <  1:3122654/91‑1 (MQ=255)

  ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                         <  1:3666528/91‑1 (MQ=255)

  ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                         <  1:2896496/91‑1 (MQ=255)

  ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                         <  1:1647661/91‑1 (MQ=255)

  ttttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTg                                                                                         <  1:1647215/91‑1 (MQ=255)

   tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGc                                                                                        >  1:4121192/1‑91 (MQ=255)

   tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                        <  1:2392413/91‑1 (MQ=255)

   tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                        <  1:2392483/91‑1 (MQ=255)

   tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                        <  1:3117676/91‑1 (MQ=255)

   tttGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGc                                                                                        <  1:2139859/91‑1 (MQ=255)

    ttgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGca                                                                                       <  1:3778961/91‑2 (MQ=255)

     tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                      <  1:168795/91‑3 (MQ=255)

     tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                      <  1:671478/91‑3 (MQ=255)

     tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                      <  1:1270419/91‑3 (MQ=255)

     tgttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCAAGCAATGcat                                                                                      <  1:3086940/91‑3 (MQ=255)

       ttgCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTgg                                                                                    >  1:2183550/1‑91 (MQ=255)

         gCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGtt                                                                                  >  1:1582090/1‑91 (MQ=255)

          ctctGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTa                                                                                 <  1:3584235/91‑1 (MQ=255)

          ctctGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTa                                                                                 >  1:1338087/1‑91 (MQ=255)

             tGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCCTAAACCATGCATTGCTTGGTTAAGc                                                                              >  1:3874619/1‑91 (MQ=255)

              ggTTGACCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCg                                                                             >  1:3221400/1‑91 (MQ=255)

               gTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATACTTTCTCTTAAGCCATGCATTGCTTGGTTAAGCGt                                                                            <  1:2411110/91‑1 (MQ=255)

                 tGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTTGTTAAGCGTAt                                                                          <  1:2295196/91‑1 (MQ=255)

                  gATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTAtt                                                                         >  1:2516946/1‑91 (MQ=255)

                   aTCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTc                                                                        >  1:3715099/1‑91 (MQ=255)

                   aTCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTc                                                                        >  1:2546713/1‑91 (MQ=255)

                    tCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCg                                                                       <  1:1695953/91‑1 (MQ=255)

                      ttCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCa                                                                     >  1:2386901/1‑91 (MQ=255)

                        cGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAtt                                                                   <  1:60221/91‑1 (MQ=255)

                         gTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAttt                                                                  <  1:1961254/91‑1 (MQ=255)

                         gTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAttt                                                                  <  1:1371317/91‑1 (MQ=255)

                         gTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCAttt                                                                  <  1:1094694/91‑1 (MQ=255)

                           tGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTg                                                                >  1:3108345/1‑91 (MQ=255)

                             atatatTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGtt                                                              <  1:3251751/91‑1 (MQ=255)

                               atatTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCa                                                            >  1:761247/1‑91 (MQ=255)

                                  tttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAtt                                                         >  1:528385/1‑91 (MQ=255)

                                  tttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAtt                                                         >  1:1307012/1‑91 (MQ=255)

                                  tttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAtt                                                         >  1:237627/1‑91 (MQ=255)

                                   ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                        >  1:2956811/1‑91 (MQ=255)

                                   ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                        >  1:1376190/1‑91 (MQ=255)

                                   ttgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGATTGGTTAAGCGTATTCGCATTTTGTTCAAttt                                                        >  1:112892/1‑91 (MQ=255)

                                    tgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtg                                                       >  1:3299731/1‑91 (MQ=255)

                                    tgctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCGTGGTTAAGCGTATTCGCATTTTGTTCAATTtg                                                       >  1:1964803/1‑91 (MQ=255)

                                     gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                      <  1:2289939/91‑1 (MQ=255)

                                     gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                      <  1:2289767/91‑1 (MQ=255)

                                     gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                      <  1:2289273/91‑1 (MQ=255)

                                     gctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtga                                                      <  1:3967567/91‑1 (MQ=255)

                                      ctatttgctTTAGTTGCTGCGTTATCTTGAATGCTCTATCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTtgat                                                     >  1:3507254/1‑91 (MQ=255)

                                          ttgctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAAc                                                 >  1:12133/1‑91 (MQ=255)

                                            gctTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTg                                               <  1:314210/91‑1 (MQ=255)

                                               ttAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAg                                            >  1:4110281/1‑91 (MQ=255)

                                                tAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGt                                           >  1:1893325/1‑91 (MQ=255)

                                                  gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                         >  1:4104438/1‑91 (MQ=255)

                                                  gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                         >  1:269403/1‑91 (MQ=255)

                                                  gTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTa                                         >  1:1196197/1‑91 (MQ=255)

                                                   ttGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAc                                        <  1:3809082/91‑1 (MQ=255)

                                                    tgctgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACt                                       <  1:705479/91‑1 (MQ=255)

                                                    tgctgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACt                                       <  1:2045402/91‑1 (MQ=255)

                                                     gctgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTa                                      <  1:3645831/91‑1 (MQ=255)

                                                       tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTaaa                                    >  1:1507819/1‑91 (MQ=255)

                                                       tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTaaa                                    <  1:3619678/91‑1 (MQ=255)

                                                       tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTaaa                                    >  1:2569021/1‑91 (MQ=255)

                                                       tgcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGCTACTaaa                                    >  1:2067416/1‑91 (MQ=255)

                                                        gcGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAg                                   >  1:256648/1‑91 (MQ=255)

                                                         cGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGt                                  >  1:1673477/1‑91 (MQ=255)

                                                          gTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGtt                                 >  1:486372/1‑91 (MQ=255)

                                                               cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAGTAAAGTTGCAGc                            <  1:3204838/91‑1 (MQ=255)

                                                               cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTAGTAAAGTTGCAGc                            <  1:3205405/91‑1 (MQ=255)

                                                               cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGc                            <  1:83780/91‑1 (MQ=255)

                                                               cTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCACATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGc                            >  1:2400368/1‑91 (MQ=255)

                                                                ttGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCt                           >  1:2205723/1‑91 (MQ=255)

                                                                 tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                          <  1:262868/91‑1 (MQ=255)

                                                                 tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                          >  1:1366848/1‑91 (MQ=255)

                                                                 tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                          <  1:3094721/91‑1 (MQ=255)

                                                                 tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                          >  1:3795181/1‑91 (MQ=255)

                                                                 tGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTg                          >  1:3795335/1‑91 (MQ=255)

                                                                  gAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGt                         <  1:3529774/91‑1 (MQ=255)

                                                                     tGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCGTTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAAc                      <  1:4139667/91‑1 (MQ=255)

                                                                     tGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAAc                      <  1:1789527/91‑1 (MQ=255)

                                                                     tGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTACAGCTGCAAc                      <  1:1919575/91‑1 (MQ=255)

                                                                       ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACtt                    >  1:1780026/1‑91 (MQ=255)

                                                                       ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACtt                    >  1:1937216/1‑91 (MQ=255)

                                                                       ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACtt                    >  1:1939007/1‑91 (MQ=255)

                                                                       ctctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTACAGCTGCAACtt                    <  1:3945801/91‑1 (MQ=255)

                                                                        tctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTg                   >  1:1528954/1‑91 (MQ=255)

                                                                         ctGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTgg                  >  1:424859/1‑91 (MQ=255)

                                                                           gTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTc                >  1:1599871/1‑91 (MQ=255)

                                                                             cTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtt              <  1:2724256/91‑1 (MQ=255)

                                                                             cTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATGTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtt              >  1:3485959/1‑91 (MQ=255)

                                                                               tAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCtttt            <  1:2838982/91‑1 (MQ=255)

                                                                                aaaCCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTa           >  1:1554437/1‑91 (MQ=255)

                                                                                aaaCCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTa           >  1:807950/1‑91 (MQ=255)

                                                                                  aCCAAGCAATGCATGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAAt         >  1:3053742/1‑91 (MQ=255)

                                                                                  aCCAAGCAATGCATGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAAt         >  1:3056632/1‑91 (MQ=255)

                                                                                     aTGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCt      <  1:1025084/91‑1 (MQ=255)

                                                                                      agCAATGCATGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTg     >  1:442412/2‑91 (MQ=255)

                                                                                       gCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGt    >  1:2777645/1‑91 (MQ=255)

                                                                                         aTTGCTTGGTTAAGCGTATTCGCCTTTTGTTCACTTTAATGAACTGTAGTTACTAAAGTAGCAGCTGTAACTTGGTCTTTTAATGCTGttt  >  1:3309071/1‑91 (MQ=255)

AGTTTTGTTGCTCTGGTTGATCTTCGTTGATATATTTGCTATTTGCTTTAGTTGCTGCGTTATCTTGAATGCTCTGTCTTAAACCATGCATTGCTTGGTTAAGCGTATTCGCATTTTGTTCAATTTGATGAACTGCAGTTACTAAAGTTGCAGCTGTAACTTGGTCTTTTAATGCTGTTT  >  USA300TCH1516_ALE/1476595‑1476774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`