| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,500,743 | T→G | 5.6% | V427V (GTA→GTC) | ebh_1 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,500,743 | 0 | T | G | 5.6% | 311.2 / 6.6 | 89 | V427V (GTA→GTC) | ebh_1 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base T (43/41); new base G (2/3); total (45/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCAGGTGTCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGTTTATTT > USA300TCH1516_ALE/1500654‑1500833 | gCAGGTGTCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTa < 1:4017904/91‑1 (MQ=255) cAGGTGTCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTAc > 1:3843469/1‑91 (MQ=255) ggTGTCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCCAAGTAATATATGACt < 1:1983123/91‑1 (MQ=255) gtCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGc < 1:2894023/91‑1 (MQ=255) gtCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGc > 1:1980919/1‑91 (MQ=255) gtCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGc > 1:1980663/1‑91 (MQ=255) gtCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGc > 1:3848351/1‑91 (MQ=255) cGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCa < 1:1860037/91‑1 (MQ=255) gCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCAc > 1:780630/1‑91 (MQ=255) cTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCCAAGTAATATATGAC‑TGGCCaac < 1:999927/91‑3 (MQ=37) cTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCCAAGTAATATATGAC‑TGGCCaac < 1:3014470/91‑3 (MQ=37) cTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACt > 1:2864219/1‑91 (MQ=255) cTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACt < 1:3828845/91‑1 (MQ=255) tCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACtt < 1:137375/91‑1 (MQ=255) ccAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTc < 1:1056370/91‑1 (MQ=255) ccAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTc < 1:1668408/91‑1 (MQ=255) cAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGCCATATATTACTTGGCCACTTCt > 1:3347982/1‑91 (MQ=255) cTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTAtt < 1:2554428/91‑1 (MQ=255) tCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTc > 1:1724048/1‑91 (MQ=255) tCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTc > 1:312577/1‑91 (MQ=255) tCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTc > 1:1560815/1‑91 (MQ=255) cACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCt < 1:1708541/91‑1 (MQ=255) cTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTa > 1:2314081/1‑91 (MQ=255) tAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTAt > 1:3166117/1‑91 (MQ=255) tAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTAt < 1:909916/91‑1 (MQ=255) tAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTAt < 1:909059/91‑1 (MQ=255) tAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTAt < 1:1375186/91‑1 (MQ=255) aaaTAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTAttt < 1:1028118/91‑1 (MQ=255) aaaTAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTAttt < 1:1509945/91‑1 (MQ=255) ataatCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTa > 1:4083121/1‑91 (MQ=255) taatCACTCGCTCTAGATAAATCTTATAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTaa > 1:2234543/1‑91 (MQ=255) taatCACTCGCTCTAGATAAATCTTATAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTaa > 1:2234263/1‑91 (MQ=255) atCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAg > 1:3346127/1‑91 (MQ=255) atCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAg > 1:3416357/1‑91 (MQ=255) atCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAg > 1:1802706/1‑91 (MQ=255) tCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGt > 1:3448311/1‑91 (MQ=255) cACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTc > 1:2490909/1‑91 (MQ=255) aCTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCa > 1:2000935/1‑91 (MQ=255) aCTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCa > 1:2001143/1‑91 (MQ=255) cTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCAt > 1:626093/1‑91 (MQ=255) tCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCAtg < 1:4079993/91‑1 (MQ=255) cGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCAtgt > 1:3231279/1‑91 (MQ=255) gCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCAtgtg < 1:1621410/91‑1 (MQ=255) ctctAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGa > 1:67278/1‑91 (MQ=255) gATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGAt > 1:3241646/1‑91 (MQ=255) gATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGAt > 1:823369/1‑91 (MQ=255) aTAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATg < 1:2058761/91‑1 (MQ=255) aTAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATg < 1:137081/91‑1 (MQ=255) tAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGa > 1:1950037/1‑91 (MQ=255) aaaTCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAg > 1:2996082/1‑91 (MQ=255) aaaTCTTCTAAAGAATAAATATGGACAGTTGGCA‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAg < 1:3396257/91‑1 (MQ=255) aaTCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAgg < 1:3756338/91‑1 (MQ=255) aaTCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAgg < 1:1802096/91‑1 (MQ=255) aTCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGa < 1:4049620/91‑1 (MQ=255) tcttctAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGAt < 1:2345796/91‑1 (MQ=255) tcttctAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGAt < 1:2035029/91‑1 (MQ=255) ttctAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCa > 1:3614758/1‑91 (MQ=255) aaaGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTa > 1:4121465/1‑91 (MQ=255) aaGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTAc < 1:1005235/91‑1 (MQ=255) aaGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTAc < 1:1005212/91‑1 (MQ=255) gAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTc > 1:1136985/1‑91 (MQ=255) gAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTc > 1:3929816/1‑91 (MQ=255) aataaataTGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTcc < 1:2355864/91‑1 (MQ=255) ataaataTGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCa > 1:1439747/1‑91 (MQ=255) aaataTGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCata < 1:507487/91‑1 (MQ=255) aaataTGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCata < 1:1622085/91‑1 (MQ=255) aataTGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCatat > 1:3140207/1‑91 (MQ=255) atatGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATAtt > 1:633381/1‑91 (MQ=255) atatGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATAtt > 1:633326/1‑91 (MQ=255) tatGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTc < 1:1630903/91‑1 (MQ=255) tGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTccc > 1:3029468/1‑91 (MQ=255) tGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTccc > 1:3669217/1‑91 (MQ=255) cAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACAt > 1:1209976/1‑91 (MQ=255) cAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACAt < 1:1628797/91‑1 (MQ=255) ggCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATttgtt > 1:17605/1‑91 (MQ=255) ggCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATttgtt < 1:492262/91‑1 (MQ=255) ccaaGTAATATATGAC‑TGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGa > 1:245138/4‑91 (MQ=255) c‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGaa < 1:2328287/91‑1 (MQ=255) gTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGt < 1:32394/91‑1 (MQ=255) gTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGt < 1:1859103/91‑1 (MQ=255) tCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTg < 1:761176/91‑1 (MQ=255) tCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTg < 1:1089740/91‑1 (MQ=255) cATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTgg > 1:1920270/1‑91 (MQ=255) cATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTgg > 1:1920284/1‑91 (MQ=255) cATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTgg < 1:2030698/91‑1 (MQ=255) aatatatGAC‑TGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGt > 1:3413149/2‑91 (MQ=255) atatatTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGt < 1:4057913/91‑1 (MQ=255) atatatTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGt < 1:1396772/91‑1 (MQ=255) tatatTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGtt < 1:805055/91‑1 (MQ=255) ttACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGTTTAtt < 1:1988922/91‑1 (MQ=255) tACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGTTTAttt > 1:1440206/1‑91 (MQ=255) | GCAGGTGTCGCTCCAGCTTCACTAAAATAATCACTCGCTCTAGATAAATCTTCTAAAGAATAAATATGGACAGTTGGCC‑AGTCATATATTACTTGGCCACTTCTATTTCTTATTTTAAAGTCATGTGAACGATGAGGATCATTTACTCCATATTCCCACATTTGTTGAAGTGGTTTATTT > USA300TCH1516_ALE/1500654‑1500833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |