BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,526,532	T→A	7.0%	K471* (AAA→TAA)	dinG_1 ←	putative ATP‑dependent helicase DinG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,526,532	0	T	A	7.0%	487.0 / 21.8	142	K471* (AAA→TAA)	dinG_1	putative ATP‑dependent helicase DinG
Reads supporting (aligned to +/- strand): ref base T (54/78); new base A (4/6); total (58/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 146 total aligned reads displayed.

ATTTTTCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAATCATCAAACAA  >  USA300TCH1516_ALE/1526442‑1526622

aTTTTTCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATAttt                                                                                            >  1:2784206/1‑91 (MQ=255)

aTTTTTCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATAttt                                                                                            >  1:2944229/1‑91 (MQ=255)

  ttttCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTaa                                                                                          <  1:3385728/91‑1 (MQ=255)

   tttCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTAAat                                                                                         <  1:618494/91‑1 (MQ=255)

   tttCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTAAat                                                                                         <  1:2643712/91‑1 (MQ=255)

   tttCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTAAat                                                                                         <  1:2969535/91‑1 (MQ=255)

    ttCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAata                                                                                        <  1:2383896/91‑1 (MQ=255)

    tggaaaaaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAata                                                                                        >  1:2256832/5‑91 (MQ=255)

     tCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAatat                                                                                       >  1:2485600/1‑91 (MQ=255)

     tCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTAAatat                                                                                       <  1:2132919/91‑1 (MQ=255)

     tCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCGGATATTAAatat                                                                                       <  1:3203097/91‑1 (MQ=255)

     ggaaaaaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAatat                                                                                       >  1:3128366/4‑91 (MQ=255)

      ccAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATc                                                                                      <  1:301903/91‑1 (MQ=255)

      ccAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATc                                                                                      >  1:2592740/1‑91 (MQ=255)

        aaaaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAg                                                                                    <  1:2144100/91‑1 (MQ=255)

         aaaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGc                                                                                   <  1:1637202/91‑1 (MQ=255)

         aaaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGc                                                                                   <  1:142633/91‑1 (MQ=255)

         aaaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGc                                                                                   <  1:2353803/91‑1 (MQ=255)

          aaTTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCa                                                                                  <  1:194318/91‑1 (MQ=255)

             tCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaa                                                                               >  1:2680227/1‑91 (MQ=255)

             tCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaa                                                                               <  1:557821/91‑1 (MQ=255)

                ctggtTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATTTCAGCATaactc                                                                            <  1:3207495/91‑1 (MQ=255)

                ctgctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactc                                                                            >  1:2202174/1‑91 (MQ=255)

                ctgctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactc                                                                            <  1:1140109/91‑1 (MQ=255)

                ctgctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactc                                                                            >  1:2074296/1‑91 (MQ=255)

                ctgctTTTCCAGTTGATCAATTGCCTTTAACAAGTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactc                                                                            <  1:3401351/91‑1 (MQ=255)

                ctgctTTTCCAGTTGATCAATTGCCTTTAACAAGTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactc                                                                            <  1:3401242/91‑1 (MQ=255)

                 tgctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactca                                                                           >  1:685474/1‑91 (MQ=255)

                  gctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaa                                                                          >  1:4057711/1‑91 (MQ=255)

                   ctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaac                                                                         <  1:1373788/91‑1 (MQ=255)

                   ctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaac                                                                         <  1:1460768/91‑1 (MQ=255)

                   ctTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaac                                                                         <  1:1734246/91‑1 (MQ=255)

                     tttCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaactc                                                                       <  1:864405/91‑1 (MQ=255)

                     tttCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaactc                                                                       <  1:864404/91‑1 (MQ=255)

                     tttCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATaactcaactc                                                                       <  1:864302/91‑1 (MQ=255)

                       tCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCAt                                                                     <  1:3119988/91‑1 (MQ=255)

                       tCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCAt                                                                     <  1:3118796/91‑1 (MQ=255)

                        ccAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCAtt                                                                    >  1:3261901/1‑91 (MQ=255)

                        ccAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCAtt                                                                    >  1:2621972/1‑91 (MQ=255)

                           gTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGt                                                                 <  1:2123734/91‑1 (MQ=255)

                            ttGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTc                                                                <  1:221562/91‑1 (MQ=255)

                             tGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCa                                                               <  1:378027/91‑1 (MQ=255)

                              gATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCAc                                                              <  1:2715346/91‑1 (MQ=255)

                               aTCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACt                                                             >  1:1689460/1‑91 (MQ=255)

                                 cAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTg                                                           >  1:2219091/1‑91 (MQ=255)

                                  aaTTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGa                                                          <  1:2717521/91‑1 (MQ=255)

                                   aTTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGAt                                                         <  1:3562715/91‑1 (MQ=255)

                                    ttGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATAAAACCAAGCTGATCTTTAATATCAGCATAACTCAACTCATTTGTCACTTGAtt                                                        >  1:2014012/1‑91 (MQ=255)

                                     tGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGAttt                                                       >  1:1516739/1‑91 (MQ=255)

                                     tGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGAttt                                                       >  1:1517115/1‑91 (MQ=255)

                                       tcTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTaa                                                     >  1:1283865/2‑91 (MQ=255)

                                        cTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAg                                                    >  1:1892853/1‑91 (MQ=255)

                                        cTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAg                                                    <  1:3333310/91‑1 (MQ=255)

                                         tttAACAATTTTTCATTTTCGTTTTCACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAgg                                                   <  1:3870882/91‑1 (MQ=255)

                                          ttAGCAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTCAGGc                                                  <  1:3275965/91‑1 (MQ=255)

                                           tAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCa                                                 >  1:455868/1‑91 (MQ=255)

                                                aTTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTc                                            <  1:455038/91‑1 (MQ=255)

                                                  ttttCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTg                                          >  1:3466594/1‑91 (MQ=255)

                                                  ttttCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTg                                          >  1:3466404/1‑91 (MQ=255)

                                                   tttCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTgg                                         <  1:4102216/91‑1 (MQ=255)

                                                    ttCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGt                                        >  1:2169641/1‑91 (MQ=255)

                                                      cATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTaa                                      <  1:2329957/91‑1 (MQ=255)

                                                      cATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTaa                                      <  1:2329906/91‑1 (MQ=255)

                                                       aTTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAg                                     >  1:3569261/1‑91 (MQ=255)

                                                       aTTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAg                                     <  1:692002/91‑1 (MQ=255)

                                                       aTTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAg                                     >  1:3569728/1‑91 (MQ=255)

                                                        ttttCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGc                                    >  1:2489621/1‑91 (MQ=255)

                                                         tttCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCg                                   <  1:3888461/91‑1 (MQ=255)

                                                         tttCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCg                                   <  1:2924051/91‑1 (MQ=255)

                                                             gTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGAtgt                               >  1:2875264/1‑91 (MQ=255)

                                                                 ttACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCtt                           >  1:2532517/1‑91 (MQ=255)

                                                                 ttACCAATCAAAACAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCtt                           >  1:3196630/1‑91 (MQ=255)

                                                                   aCCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCa                         >  1:313511/1‑91 (MQ=255)

                                                                   aCCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCa                         >  1:4034892/1‑91 (MQ=255)

                                                                      aaTCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATcc                      <  1:2164149/91‑1 (MQ=255)

                                                                        tCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCAc                    <  1:1815597/91‑1 (MQ=255)

                                                                        tCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCAc                    <  1:3711711/91‑1 (MQ=255)

                                                                         cAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACt                   <  1:2287022/91‑1 (MQ=255)

                                                                         cAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACt                   <  1:2286417/91‑1 (MQ=255)

                                                                           aaCCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGGTTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACtat                 <  1:3592975/91‑1 (MQ=255)

                                                                            aCCAAGCTGATATTTAATATCAGCATAACTCAACTCAGTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACtata                >  1:3510297/1‑91 (MQ=255)

                                                                            aCCAAGCTGAAATTTAATATCAGCATAACTCAACTCATTCGTCACTGGATTAAAAGCTTAGTCTGGTAAGCGATATGCTTCATCTACtata                >  1:4063824/1‑91 (MQ=255)

                                                                              cAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACa              >  1:3836188/1‑91 (MQ=255)

                                                                              cAAGCTGATATTTAATATCAGCATAACTCAACTCATTCGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACa              >  1:3505793/1‑91 (MQ=255)

                                                                                aGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAat            <  1:1553566/91‑1 (MQ=255)

                                                                                aGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAat            <  1:1041588/91‑1 (MQ=255)

                                                                                  cTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatca          <  1:3746954/91‑1 (MQ=255)

                                                                                   tGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcat         <  1:1288535/91‑1 (MQ=255)

                                                                                   tGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcat         >  1:1198477/1‑91 (MQ=255)

                                                                                   tGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcat         <  1:2524277/91‑1 (MQ=255)

                                                                                   ggATATTTAATATCAGCATAACTCCACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcat         <  1:2084830/90‑1 (MQ=255)

                                                                                    gATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcatc        >  1:4005118/1‑91 (MQ=255)

                                                                                    gATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcatc        <  1:2813747/91‑1 (MQ=255)

                                                                                    gATATTAAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcatc        >  1:1637504/1‑91 (MQ=255)

                                                                                    gATATTAAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAatcatc        >  1:1637160/1‑91 (MQ=255)

                                                                                     atatTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAAtcatca       <  1:1662692/91‑1 (MQ=255)

                                                                                     atatTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAAtcatca       <  1:1663240/91‑1 (MQ=255)

                                                                                     atatTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAAtcatca       >  1:3833819/1‑91 (MQ=255)

                                                                                       atTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAATCATCaaa     <  1:2369287/91‑1 (MQ=255)

                                                                                         ttAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAATCATCAAACa   <  1:850294/91‑1 (MQ=255)

ATTTTTCCAAAATTCGCTGCTTTTCCAGTTGATCAATTGCCTTTAACAATTTTTCATTTTCGTTTTTACCAATCAAACCAAGCTGATATTTAATATCAGCATAACTCAACTCATTTGTCACTTGATTTAAGGCATAGTCTGGTAAGCGATGTGCTTCATCCACTATACAATCATCAAACAA  >  USA300TCH1516_ALE/1526442‑1526622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`