BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,934,773	T→A	6.0%	K159* (AAA→TAA)	USA300HOU_RS09605 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,934,773	0	T	A	6.0%	645.2 / 19.7	185	K159* (AAA→TAA)	USA300HOU_RS09605	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (71/101); new base A (4/8); total (76/109)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

AAATTTCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGATA  >  USA300TCH1516_ALE/1934683‑1934863

aaaTTTCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATAtttt                                                                                            >  1:982168/1‑91 (MQ=255)

aaaTTTCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATAtttt                                                                                            <  1:3228466/91‑1 (MQ=255)

aaaTTTCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATAtttt                                                                                            >  1:3624431/1‑91 (MQ=255)

    ttCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTg                                                                                        <  1:2231965/91‑1 (MQ=255)

     tCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGa                                                                                       >  1:1331882/1‑91 (MQ=255)

       aCTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGaaa                                                                                     >  1:807670/1‑91 (MQ=255)

       aCTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGaaa                                                                                     <  1:3114925/91‑1 (MQ=255)

        cTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAAt                                                                                    >  1:2097682/1‑91 (MQ=255)

           aaGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTATTTGAATTTCa                                                                                 <  1:113106/91‑1 (MQ=255)

            aGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaa                                                                                >  1:2160130/1‑91 (MQ=255)

            aGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaa                                                                                >  1:1249833/1‑91 (MQ=255)

                 tttttCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaaataaa                                                                           <  1:857238/91‑1 (MQ=255)

                 tttttCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaaataaa                                                                           >  1:721222/1‑91 (MQ=255)

                 tttttCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaaataaa                                                                           <  1:760669/91‑1 (MQ=255)

                 tttttCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaaataaa                                                                           <  1:759985/91‑1 (MQ=255)

                  ttttcttttcTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCaaataaat                                                                          >  1:3198035/1‑91 (MQ=255)

                     tcttttcttCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATAtt                                                                       >  1:2312313/1‑91 (MQ=255)

                      cttttcttCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAACATTc                                                                      >  1:3455041/1‑91 (MQ=255)

                       ttttcttCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTATTTGAATTTCAAAAAAATATTCt                                                                     <  1:1574836/91‑1 (MQ=37)

                         ttcttcAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCttt                                                                   >  1:4082364/1‑91 (MQ=255)

                         ttcttcAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCttt                                                                   >  1:3972056/1‑91 (MQ=255)

                                aaCTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTAtt                                                            <  1:1403949/91‑1 (MQ=255)

                                 aCTCTTCATCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTAttt                                                           <  1:2067818/91‑1 (MQ=255)

                                  ctctTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTAtttt                                                          <  1:1871426/91‑1 (MQ=255)

                                    cttcttCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGa                                                        >  1:1162444/1‑91 (MQ=255)

                                    cttcttCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGa                                                        >  1:1478988/1‑91 (MQ=255)

                                      tcttcCAGTTCCAAATAGTTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGAtt                                                      <  1:3549135/91‑1 (MQ=255)

                                      tcttcCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGAtt                                                      >  1:1895026/1‑91 (MQ=255)

                                      tcttcCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGAtt                                                      <  1:1688907/91‑1 (MQ=255)

                                      tcttcCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGAtt                                                      >  1:3577321/1‑91 (MQ=255)

                                       cttcCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGAttt                                                     <  1:3177149/91‑1 (MQ=255)

                                          ccAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCAt                                                  <  1:3511206/91‑1 (MQ=255)

                                            aGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCt                                                <  1:2747767/91‑1 (MQ=255)

                                                ccAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATc                                            <  1:3268872/91‑1 (MQ=255)

                                                ccAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATc                                            <  1:1446849/91‑1 (MQ=255)

                                                ccAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATc                                            <  1:1225191/91‑1 (MQ=255)

                                                ccAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATc                                            >  1:3127510/1‑91 (MQ=255)

                                                ccAAATAATGATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAGTAAATATTCTTTGCGTATTTTGATTTCATCTTATc                                            <  1:3974930/91‑1 (MQ=255)

                                                   aataatTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTaa                                         >  1:697232/1‑91 (MQ=255)

                                                      aatTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAat                                      >  1:1440548/1‑91 (MQ=255)

                                                      aatTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAat                                      >  1:1805659/1‑91 (MQ=255)

                                                      aatTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAat                                      >  1:1741300/1‑91 (MQ=255)

                                                        ttattGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAatat                                    <  1:534290/91‑1 (MQ=255)

                                                        ttattGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAatat                                    <  1:3124045/91‑1 (MQ=255)

                                                         tattGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATc                                   <  1:1621261/91‑1 (MQ=255)

                                                           ttGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAt                                 >  1:2776676/1‑91 (MQ=255)

                                                            tGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAtt                                >  1:3750835/1‑91 (MQ=255)

                                                             gACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAttt                               <  1:3414242/91‑1 (MQ=255)

                                                             gACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAttt                               <  1:2148938/91‑1 (MQ=255)

                                                              aCTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAtttt                              <  1:3728040/91‑1 (MQ=255)

                                                              aCTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAtttt                              <  1:2956102/91‑1 (MQ=255)

                                                               cTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTc                             <  1:564350/91‑1 (MQ=255)

                                                                tGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCg                            <  1:2053120/91‑1 (MQ=255)

                                                                 gATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGa                           >  1:1007925/1‑91 (MQ=255)

                                                                 gATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGa                           <  1:307166/91‑1 (MQ=255)

                                                                  aTTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGat                          <  1:3136863/91‑1 (MQ=255)

                                                                  aTTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGat                          <  1:2653681/91‑1 (MQ=255)

                                                                   ttGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGata                         <  1:3307437/91‑1 (MQ=255)

                                                                   ttGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGata                         <  1:1282810/91‑1 (MQ=255)

                                                                    tGTTCTAGATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCAGTTTCGatat                        <  1:2087561/91‑1 (MQ=255)

                                                                    tGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGatat                        >  1:2392029/1‑91 (MQ=255)

                                                                      ttCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATaa                      >  1:461171/1‑91 (MQ=255)

                                                                       tCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAAc                     >  1:2192733/1‑91 (MQ=255)

                                                                       tCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAAc                     >  1:1934902/1‑91 (MQ=255)

                                                                       tCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAAc                     >  1:67324/1‑91 (MQ=255)

                                                                       tCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAAc                     <  1:810060/91‑1 (MQ=255)

                                                                        cTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAAcc                    >  1:2592430/1‑91 (MQ=255)

                                                                         tAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCt                   <  1:3701710/91‑1 (MQ=255)

                                                                         tAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCt                   >  1:1656862/1‑91 (MQ=255)

                                                                         tAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCt                   <  1:1838/91‑1 (MQ=255)

                                                                         tAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCt                   <  1:1351948/91‑1 (MQ=255)

                                                                          aaaTTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCtt                  <  1:2577378/91‑1 (MQ=255)

                                                                          aaaTTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCtt                  <  1:2254408/91‑1 (MQ=255)

                                                                           aaTTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCttt                 <  1:2433988/91‑1 (MQ=255)

                                                                           aaTTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCttt                 <  1:2260426/91‑1 (MQ=255)

                                                                            aTTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCtttt                <  1:2533595/91‑1 (MQ=255)

                                                                            aTTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCtttt                <  1:2153543/91‑1 (MQ=255)

                                                                             ttAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTa               >  1:3155921/1‑91 (MQ=255)

                                                                             gcaaagaATATTTATTTGAATTTCAAAAAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTa               >  1:4053163/5‑91 (MQ=25)

                                                                              tAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTaa              <  1:3909025/91‑1 (MQ=255)

                                                                               agagAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGCTTTCATCTTATCTAAAATATCATTTTCGACATAACCTTTTAAt             >  1:4148575/1‑91 (MQ=255)

                                                                               agagAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAAt             >  1:3031825/1‑91 (MQ=255)

                                                                               agagAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAAt             >  1:1598164/1‑91 (MQ=255)

                                                                                gagaATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAAtt            >  1:1049908/1‑91 (MQ=255)

                                                                                 agaATATTTCTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTCTTAATTc           >  1:2080964/1‑91 (MQ=255)

                                                                                 agaATATTTATTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTCTTAATTc           >  1:2080878/1‑91 (MQ=255)

                                                                                   aaTATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCtt         <  1:2358174/91‑1 (MQ=255)

                                                                                    atatTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCttt        <  1:2996681/91‑1 (MQ=255)

                                                                                      atttatttGAATTTCAAAAAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTa      >  1:2657674/1‑91 (MQ=38)

                                                                                      atTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTa      <  1:4115585/91‑1 (MQ=255)

                                                                                      atTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTa      <  1:3273523/91‑1 (MQ=255)

                                                                                      atTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTa      <  1:2540631/91‑1 (MQ=255)

                                                                                       tttttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAg     >  1:586318/1‑91 (MQ=255)

                                                                                       tttttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAg     <  1:6471/91‑1 (MQ=255)

                                                                                       tttttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAg     <  1:681625/91‑1 (MQ=255)

                                                                                        ttttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGa    >  1:2903173/1‑91 (MQ=255)

                                                                                         tttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGat   <  1:2380406/91‑1 (MQ=255)

                                                                                         tttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGat   <  1:879561/91‑1 (MQ=255)

                                                                                          ttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGata  <  1:1413614/91‑1 (MQ=255)

                                                                                          ttttGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGata  <  1:2499063/91‑1 (MQ=255)

AAATTTCACTTAAGTATTTTTTCTTTTCTTCAAACTCTTCTTCCAGTTCCAAATAATTATTGACTGATTGTTCTAAATTAGAGAATATTTTTTTGAAATTCAAATAAATATTCTTTGCGTATTTTGATTTCATCTTATCTAAAATATCATTTTCGATATAACCTTTTAATTCTTTTAGATA  >  USA300TCH1516_ALE/1934683‑1934863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`