BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,050,876	A→G	5.0%	L473L (TTA→CTA)	pcrA ←	ATP‑dependent DNA helicase PcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,050,876	0	A	G	5.0%	389.9 / 7.9	119	L473L (TTA→CTA)	pcrA	ATP‑dependent DNA helicase PcrA
Reads supporting (aligned to +/- strand): ref base A (49/64); new base G (3/3); total (52/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 125 total aligned reads displayed.

ACTTCGAGATTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATCAGC  >  USA300TCH1516_ALE/2050787‑2050966

 cTTCGAGATTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTaaaa                                                                                          <  1:3979466/91‑1 (MQ=255)

   tCGAGATTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTACTTCATGAATTTCTAAAAAt                                                                                        <  1:1487671/91‑1 (MQ=255)

     gagaTTCTAGTGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTTTAGAAATTc                                                                                      <  1:3976037/91‑1 (MQ=255)

     gagaTTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTTTAGAAATTc                                                                                      <  1:3980288/91‑1 (MQ=255)

     gagaTTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTc                                                                                      >  1:2667556/1‑91 (MQ=255)

      agaTTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGCAATACTTCATCAAATATTTCATGAATTTCTAAAGATTCt                                                                                     >  1:3857169/1‑91 (MQ=255)

       gaTTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCtt                                                                                    <  1:1704533/91‑1 (MQ=255)

        aTTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTg                                                                                   <  1:2517633/91‑1 (MQ=255)

         ttCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGCCTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGt                                                                                  >  1:2396413/1‑91 (MQ=255)

         ttCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGt                                                                                  >  1:723771/1‑91 (MQ=255)

         ttCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGt                                                                                  <  1:2155233/91‑1 (MQ=255)

           cTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTc                                                                                >  1:3140744/1‑91 (MQ=255)

           cTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTc                                                                                >  1:243786/1‑91 (MQ=255)

           cTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTc                                                                                >  1:4026332/1‑91 (MQ=255)

           cTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTAGTTc                                                                                >  1:3450760/1‑91 (MQ=255)

           cTAATGTATTTTCACGTTCAAGCATCTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTc                                                                                <  1:3871510/91‑1 (MQ=255)

            tAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCt                                                                               <  1:1350345/91‑1 (MQ=255)

              aTGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCttt                                                                             >  1:3943048/1‑91 (MQ=255)

              aTGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCttt                                                                             >  1:425263/1‑91 (MQ=255)

               tGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCtttt                                                                            <  1:1168148/91‑1 (MQ=255)

                gTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTa                                                                           <  1:1150415/91‑1 (MQ=255)

                gTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTa                                                                           >  1:1741012/1‑91 (MQ=255)

                  aTTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATc                                                                         <  1:3526936/91‑1 (MQ=255)

                  aTTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATc                                                                         <  1:3044156/91‑1 (MQ=255)

                  aTTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATc                                                                         <  1:3043422/91‑1 (MQ=255)

                       cACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCt                                                                    >  1:104903/1‑91 (MQ=255)

                        aCGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCtt                                                                   <  1:1761355/91‑1 (MQ=255)

                         cGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCttt                                                                  >  1:1557847/1‑91 (MQ=255)

                         cGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCttt                                                                  >  1:4045402/1‑91 (MQ=255)

                          gTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTg                                                                 <  1:1396370/91‑1 (MQ=255)

                           ttCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTgg                                                                >  1:2886528/1‑91 (MQ=255)

                            tCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGa                                                               >  1:2832161/1‑91 (MQ=255)

                             ctaGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGAt                                                              >  1:3932068/3‑91 (MQ=255)

                                     tctcGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGt                                                      >  1:3799127/1‑91 (MQ=255)

                                     tctcGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGt                                                      >  1:3798271/1‑91 (MQ=255)

                                       tcGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCACGCTTTGGATTAACTCGTaa                                                    >  1:2740354/1‑91 (MQ=255)

                                        cGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTaaa                                                   >  1:2871616/1‑91 (MQ=255)

                                        cGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTaaa                                                   >  1:2871024/1‑91 (MQ=255)

                                         gATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTaaaa                                                  >  1:1351257/1‑91 (MQ=255)

                                           tAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAAt                                                >  1:2191872/1‑91 (MQ=255)

                                           tAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCAAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAAt                                                <  1:3315392/91‑1 (MQ=255)

                                              ccAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTaa                                             <  1:2682782/91‑1 (MQ=255)

                                               cAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGCTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAg                                            >  1:320084/1‑91 (MQ=255)

                                                aGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGa                                           <  1:4061961/91‑1 (MQ=255)

                                                aGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGa                                           <  1:1634909/91‑1 (MQ=255)

                                                aGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGa                                           >  1:48559/1‑91 (MQ=255)

                                                  aCTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGaca                                         <  1:1841078/91‑1 (MQ=255)

                                                   cTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGacac                                        <  1:2041507/91‑1 (MQ=255)

                                                   cTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGacac                                        <  1:2041106/91‑1 (MQ=255)

                                                   cTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGacac                                        >  1:4055550/1‑91 (MQ=255)

                                                    ttttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACAct                                       >  1:1083110/1‑91 (MQ=255)

                                                     tttGTAATACTTCATCAACTATTTCATGAATTTGTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActc                                      <  1:3399448/91‑1 (MQ=255)

                                                     tttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActc                                      <  1:1044164/91‑1 (MQ=255)

                                                     tttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActc                                      <  1:3008666/91‑1 (MQ=255)

                                                      ttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGacacaag                                     <  1:1260516/91‑4 (MQ=255)

                                                      ttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGacacaag                                     <  1:2690059/91‑4 (MQ=255)

                                                      ttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActct                                     <  1:768283/91‑1 (MQ=255)

                                                      ttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActct                                     <  1:766674/91‑1 (MQ=255)

                                                      ttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActct                                     <  1:747368/91‑1 (MQ=255)

                                                      ttGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACActct                                     <  1:1186227/91‑1 (MQ=255)

                                                       tGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCtt                                    >  1:1879340/1‑91 (MQ=255)

                                                       tGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCtt                                    >  1:3137936/1‑91 (MQ=255)

                                                        gTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTtg                                   >  1:3553993/1‑91 (MQ=255)

                                                        gTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTtg                                   >  1:3725565/1‑91 (MQ=255)

                                                        gTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTtg                                   <  1:3792322/91‑1 (MQ=255)

                                                        gTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTAGCATTAACTCGTAAAAATTAAGACACTCTtg                                   >  1:3321475/1‑91 (MQ=255)

                                                          aaTACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTtgtg                                 <  1:2751258/91‑1 (MQ=255)

                                                          aaTACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCCTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTtgtg                                 <  1:3294321/91‑1 (MQ=255)

                                                          aaTACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCCTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTtgtg                                 <  1:3294272/91‑1 (MQ=255)

                                                             aCTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACACTTGTATCa                              >  1:3733469/1‑91 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:3837103/91‑1 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:1029302/91‑1 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:2207088/91‑1 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:3836333/91‑1 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:3238813/91‑1 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:2724523/91‑1 (MQ=255)

                                                              cTTCATCAACTATTTCATGAATTTCTAAAAATCCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAc                             <  1:1443454/91‑1 (MQ=255)

                                                               ttCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCAcc                            >  1:305509/1‑91 (MQ=255)

                                                                  atcaACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCttt                         <  1:4025305/91‑1 (MQ=255)

                                                                    caACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCttttt                       <  1:2139331/91‑1 (MQ=255)

                                                                       cTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGa                    <  1:3739102/91‑1 (MQ=255)

                                                                       cTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGa                    <  1:3739024/91‑1 (MQ=255)

                                                                        tATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGAc                   <  1:1188221/91‑1 (MQ=255)

                                                                           ttCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACTCTCTTGTGTCACCTTTTTTCACAAt                >  1:3867253/1‑91 (MQ=255)

                                                                            tCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATc               <  1:39846/91‑1 (MQ=255)

                                                                            tCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATCAAGACACTCTTGTGTCACCTTTTTTGACAATc               >  1:3544080/1‑91 (MQ=255)

                                                                             cATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATcc              <  1:165992/91‑1 (MQ=255)

                                                                             cATGAATTTCTAAAAATTCTTGTTCTTTTAGCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATcc              <  1:3538183/91‑1 (MQ=255)

                                                                               tGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGa            >  1:2311907/1‑91 (MQ=255)

                                                                               tGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGa            <  1:2774966/91‑1 (MQ=255)

                                                                                gAATTTTTAGAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGAt           >  1:3321329/1‑91 (MQ=255)

                                                                                gAATTTTTAGAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGAt           >  1:2271068/1‑91 (MQ=255)

                                                                                gAATTTTTAGAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGAt           >  1:2271224/1‑91 (MQ=255)

                                                                                  aTTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATaa         <  1:541300/91‑1 (MQ=255)

                                                                                    ttCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATaaaa       <  1:2038649/91‑1 (MQ=255)

                                                                                      cTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATc     >  1:1818093/1‑91 (MQ=255)

                                                                                       tAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATCa    >  1:3843985/1‑91 (MQ=255)

                                                                                        aaaaaTTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATCAg   <  1:2918139/91‑1 (MQ=255)

                                                                                        aaaaaTTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATCAg   >  1:498367/1‑91 (MQ=255)

                                                                                         aaaaTTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATCAGc  >  1:327866/1‑91 (MQ=255)

ACTTCGAGATTCTAATGTATTTTCACGTTCAAGCATTTCTCGATAGCCAGACTTTTGTAATACTTCATCAACTATTTCATGAATTTCTAAAAATTCTTGTTCTTTTATCAAGCTTTGGATTAACTCGTAAAAATTAAGACACTCTTGTGTCACCTTTTTTGACAATCCGATAAAATCAGC  >  USA300TCH1516_ALE/2050787‑2050966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`